Why one species in New Zealand, Pugetia delicatissima (Kallymeniaceae, Rhodophyta), should become two new genera, Judithia gen. nov. and Wendya gen. nov.

10.26686/wgtn.12847622.v1 ◽

2020 ◽

Author(s):

R D’Archino ◽

SM Lin ◽

PW Gabrielson ◽

Giuseppe Zuccarello

Keyword(s):

New Zealand ◽

Dna Sequences ◽

Phylogenetic Analyses ◽

Supporting Cell ◽

Rbcl Gene ◽

Cortical Cells ◽

Branch System ◽

The Family ◽

Carpogonial Branch ◽

Subsidiary Cells

© 2015 British Phycological Society. Blade-forming red algae occur worldwide and, prior to DNA sequencing, had been notoriously difficult to identify and classify, especially when lacking critical reproductive features. This, coupled in New Zealand with many longstanding assumptions that taxa were identical to non-New Zealand species or genera, resulted in many misapplied names. Pugetia delicatissima R.E. Norris, an endemic New Zealand blade-forming species of the family Kallymeniaceae, is actually comprised of one existing and one new species belonging to two distinct genera, as established by our phylogenetic analyses of DNA sequences from the rbcL gene. Analyses of combined rbcL and LSU genes showed that neither is closely related to the generitype of Pugetia, the northern-eastern Pacific, P. fragilissima Kylin. We propose the names Judithia and Wendya for these two newly revealed genera. In addition to diagnostic rbcL and LSU sequences, Judithia is morphologically and anatomically characterized by rounded to oblong blades that do not taper basally at the stipe, loosely aggregated surface cortical cells and cystocarps lacking both a pericarp and an ostiole, all features observed in the holotype of P. delicatissima. Wendya, in contrast, is characterized by blades that taper both apically and basally, compactly arranged surface cortical cells and cystocarps that have both a pericarp and a distinct ostiole. The two genera also are distinguished from one other, as well as from Pugetia by features of pre- and post-fertilization development, including the number of subsidiary cells produced on carpogonial and auxiliary branch systems, whether subsidiary cells in the carpogonial branch system fuse with the supporting cell or not, and the site of origin of gonimoblast cells. Although small in area, New Zealand hosts ten of the 27 currently recognized genera in the Kallymeniaceae and is the southern-hemisphere region of greatest generic diversification in this family.

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Studies of Marine algae in the lesser-known families of the Gigartinales (Rhodophyta). II. The Dicranemaceae

Australian Journal of Botany ◽

10.1071/bt9770219 ◽

1977 ◽

Vol 25 (2) ◽

pp. 219 ◽

Cited By ~ 12

Author(s):

GT Kraft

Keyword(s):

Marine Algae ◽

Supporting Cell ◽

Branch System ◽

Auxiliary Cell ◽

Cell Position ◽

The Family ◽

Red Algal ◽

Carpogonial Branch ◽

Advanced Stages ◽

Fusion Cell

The red algal family Dicranemaceae (Gigartinales) has been studied with regard to its vegetative and reproductive morphology, The group is composed of two Dicranema species (D. revolutum (C. Ag.) J. Ag. and D. cincinnalis sp, nov.), Peltasta australis J. Ag., Reptataxis rhizophora (Lucas) gen. et comb. nov., and Tylotus obtusatus (Sond.) J . Ag. All except Reptataxis, from Lord Howe I., are endemic to southern Australia. The last three genera are newly added to the family, which is redefined to embrace their early gonimoblast similarities to Dicranema. The species are all multiaxial, zonately tetrasporangiate and monoecious. Dicranema, Peltasta and Reptataxis have broad, cellular cortexes and filamentous medullas, while Tylotus is pseudoparenchymatous throughout. Tetrasporangia are nemathecial in Dieranem, Reptataxis and Tylotus, but scattered in Peltasta. Spermatangia in Dicranema are formed in deeply buried catenate clusters, and are similarly derived but non-catenate in the other genera. The species are all monocarpogonial, and only Tylotus is procarpic. In none of the genera are sterile cells associated with carpogonial branches. In Tylotus the supporting cell of the carpogonial branch fuses with the presumably fertilized carpogonium and becomes the diploidized auxiliary cell. In Dicranema, Peltasta and Reptataxis, 2- or 3-celled carpogonial branches are directed to the thallus surface and presumably fertilized carpogonia fuse with an auxiliary cell not necessarily on the same cortical branch system as the supporting cell. Diploidized auxiliary cells in all four genera form small, irregular fusion cells and emit multiple, filamentous gonimoblasts. Gonimoblast growth is mostly thallus-inward in Dicranema, initially radial in Peltasta and Reptataxis, and lateral and outward in Tylotus and advanced stages of Peltasta and Reptataxis. In Dicranema, Peltasta and Reptataxis an extensive placenta of mixed and connected gonimoblast and vegetative cells forms between the fusion cell and the peripheral carpospore-producing layers. In Tylotus vegetative/gonimoblast cell connections occur mainly at the floor of the cystocarp. Carposporangia develop singly on elongate penultimate gonimoblasts in Dicranema and Tylotus, but form chains in Peltasta and Reptataxis. Cystocarps in all the genera are surrounded by thick ostiolate pericarps. The genera of the Dicranemaceae are distinct from one another on frond and holdfast habits, vegetative structure, carpogonial branch shape, auxiliary cell position, gonimoblast orientation, carposporangial size, and mature cystocarp location and cross section. None of the four genera seem obviously derived from or particularly closely related to any others outside the family, although both Peltasta and Reptataxis show some features susesting possible links to the Sarcodiaceae.

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Phylogeography of the New Zealand whelks Cominella maculosa and C. virgata

10.26686/wgtn.17064731.v1 ◽

2021 ◽

Author(s):

◽

Kerry Walton

Keyword(s):

New Zealand ◽

Dna Sequences ◽

High Frequency ◽

Phylogenetic Analyses ◽

Mitochondrial Gene ◽

Nuclear Gene ◽

Disjunct Distribution ◽

Phylogeographic Structure ◽

Interglacial Period ◽

Cook Strait

Cominella maculosa and C. virgata are common rocky shore whelk species from New Zealand. This study used DNA sequences from the mitochondrial gene cytochrome c oxidase subunit 1 (CO1) to expand an earlier unpublished dataset and examine the phylogeographic structure of both species in the Cook Strait region, of C. maculosa in the Chatham Islands, and of C. virgata in the northern North Island. Both species are found to have a considerable degree of phylogeographic structure, concordant with that reported by an earlier study and for other species with direct development. South Island sites sampled for C. maculosa had several private haplotypes and a high frequency haplotype that is shared with populations from the southern North Island. Together, these formed a ‘southern haplogroup’. Low diversity in ‘southern’ populations may reflect founder effects that would have occurred as part of a southward range expansion during the onset of the present interglacial period. The Chatham Islands samples had two haplotypes that formed a separate sub-group to the ‘southern haplogroup’, suggesting Chatham Islands populations are moderately isolated from those on mainland New Zealand but may have been founded from ‘southern’ populations relatively recently. The high frequency haplotype present in South Island samples of C. virgata is absent in Wellington samples but widespread in those from the north-eastern North Island. South Island populations may have been founded from the Hauraki Gulf through human-mediated translocation events. Phylogenetic analyses with a focus on C. virgata were conducted using the mitochondrial genes CO1 and 16SrRNA, and the nuclear gene 18S rRNA, to expand an earlier published dataset. The purported northern subspecies C. virgata brookesi does not form a monophyletic lineage and voucher specimens fluidly intergrade with the nominal subspecies, with which it is synonymised. A lectotype is designated for Buccinum lineolatum Quoy & Gaimard, 1833, for which Cominella virgata is a replacement name. Potential causes of the disjunct distribution patterns of C. virgata and other mollusc taxa are discussed with particular reference to the formation and timing of marine straits through the Auckland Isthmus and Cook Strait.

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DNA sequencing supports the reinstatement of Pycnolejeunea glauca from synonymy of Cheilolejeunea intertexta, as Cheilolejeunea glauca comb. nov. (Lejeuneaceae, Marchantiophyta)

Telopea ◽

10.7751/telopea14692 ◽

2020 ◽

Vol 23 ◽

pp. 169-179

Author(s):

Peter Beveridge ◽

David Glenny ◽

Lara Shepherd

Keyword(s):

New Zealand ◽

Dna Sequencing ◽

Dna Sequences ◽

Phylogenetic Analyses ◽

New Combination ◽

Key Features

Pycnolejeunea glauca, originally described by Stephani based on a collection in New Zealand by William Colenso, was placed by Grolle into the synonymy of the paleotropical species Cheilolejeunea intertexta with a type from Micronesia. In this study, we generated DNA sequences from a recently-collected sample of P. glauca and compared them with published sequences of C. intertexta from China. Pycnolejeunea glauca was recovered in the phylogenetic analyses as sister to C. nipponica, whereas C. intertexta grouped in another clade with C. vittata and C. streimannii. The analysis justifies the reinstatement of P. glauca as the new combination Cheilolejeunea glauca, a New Zealand endemic. Descriptions and illustrations are provided of key features of this species together with data on its position in the phylogeny of the genus.

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Two new asexual genera and six new asexual species in the family Microthyriaceae (Dothideomycetes, Ascomycota) from China

MycoKeys ◽

10.3897/mycokeys.85.70829 ◽

2021 ◽

Vol 85 ◽

pp. 1-30

Author(s):

Min Qiao ◽

Hua Zheng ◽

Ji-Shu Guo ◽

Rafael F. Castañeda-Ruiz ◽

Jian-Ping Xu ◽

...

Keyword(s):

New Taxa ◽

Dna Sequences ◽

Sequence Data ◽

Southern China ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Aquatic Hyphomycetes ◽

Internal Transcribed Spacers ◽

The Family

The family Microthyriaceae is represented by relatively few mycelial cultures and DNA sequences; as a result, the taxonomy and classification of this group of organisms remain poorly understood. During the investigation of the diversity of aquatic hyphomycetes from southern China, several isolates were collected. These isolates were cultured and sequenced and a BLAST search of its LSU sequences against data in GenBank revealed that the closest related taxa are in the genus Microthyrium. Phylogenetic analyses, based on the combined sequence data from the internal transcribed spacers (ITS) and the large subunit (LSU), revealed that these isolates represent eight new taxa in Microthyriaceae, including two new genera, Antidactylariagen. nov. and Isthmomycesgen. nov. and six new species, Antidactylaria minifimbriatasp. nov., Isthmomyces oxysporussp. nov., I. dissimilissp. nov., I. macrosporussp. nov., Triscelophorus anisopterioideussp. nov. and T. sinensissp. nov. These new taxa are described, illustrated for their morphologies and compared with similar taxa. In addition, two new combinations are proposed in this family.

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Phylogeography of the New Zealand whelks Cominella maculosa and C. virgata

10.26686/wgtn.17064731 ◽

2021 ◽

Author(s):

◽

Kerry Walton

Keyword(s):

New Zealand ◽

Dna Sequences ◽

High Frequency ◽

Phylogenetic Analyses ◽

Mitochondrial Gene ◽

Nuclear Gene ◽

Disjunct Distribution ◽

Phylogeographic Structure ◽

Interglacial Period ◽

Cook Strait

Cominella maculosa and C. virgata are common rocky shore whelk species from New Zealand. This study used DNA sequences from the mitochondrial gene cytochrome c oxidase subunit 1 (CO1) to expand an earlier unpublished dataset and examine the phylogeographic structure of both species in the Cook Strait region, of C. maculosa in the Chatham Islands, and of C. virgata in the northern North Island. Both species are found to have a considerable degree of phylogeographic structure, concordant with that reported by an earlier study and for other species with direct development. South Island sites sampled for C. maculosa had several private haplotypes and a high frequency haplotype that is shared with populations from the southern North Island. Together, these formed a ‘southern haplogroup’. Low diversity in ‘southern’ populations may reflect founder effects that would have occurred as part of a southward range expansion during the onset of the present interglacial period. The Chatham Islands samples had two haplotypes that formed a separate sub-group to the ‘southern haplogroup’, suggesting Chatham Islands populations are moderately isolated from those on mainland New Zealand but may have been founded from ‘southern’ populations relatively recently. The high frequency haplotype present in South Island samples of C. virgata is absent in Wellington samples but widespread in those from the north-eastern North Island. South Island populations may have been founded from the Hauraki Gulf through human-mediated translocation events. Phylogenetic analyses with a focus on C. virgata were conducted using the mitochondrial genes CO1 and 16SrRNA, and the nuclear gene 18S rRNA, to expand an earlier published dataset. The purported northern subspecies C. virgata brookesi does not form a monophyletic lineage and voucher specimens fluidly intergrade with the nominal subspecies, with which it is synonymised. A lectotype is designated for Buccinum lineolatum Quoy & Gaimard, 1833, for which Cominella virgata is a replacement name. Potential causes of the disjunct distribution patterns of C. virgata and other mollusc taxa are discussed with particular reference to the formation and timing of marine straits through the Auckland Isthmus and Cook Strait.

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Phylogeny of the fern family Aspleniaceae in Australasia and the south-western Pacific

Australian Systematic Botany ◽

10.1071/sb14043 ◽

2014 ◽

Vol 27 (6) ◽

pp. 355 ◽

Cited By ~ 23

Author(s):

Daniel J. Ohlsen ◽

Leon R. Perrie ◽

Lara D. Shepherd ◽

Patrick J. Brownsey ◽

Michael J. Bayly

Keyword(s):

New Zealand ◽

East Asia ◽

Phylogenetic Analyses ◽

Western Pacific ◽

South East Asia ◽

The South ◽

Widespread Species ◽

Species Complexes ◽

The Family

Aspleniaceae is one of the largest fern families. It is species-rich in Australasia and the south-western Pacific (ASWP), where approximately 115 species occur. In the current study, the chloroplast regions rbcL, trnL–trnF and rps4–trnS were sequenced for 100 Aspleniaceae samples from ASWP. These data were combined with published sequences for species from New Zealand and other regions for phylogenetic analyses. Species of Aspleniaceae from ASWP were placed in six of the eight previously identified inter-continental clades. The majority of species from ASWP were placed in two of these clades, with the remaining four clades each being represented by three or fewer species. Strong biogeographic affinities with South-east Asia were observed and immigration, rather than local radiations of endemic taxa, appears to have made a more important contribution to patterns of diversity in ASWP. This study supports the current taxonomic practice of recognising two genera, Asplenium L. and Hymenasplenium Hayata, in Aspleniaceae, and identifies future taxonomic work required for the family in this region, including potential synonymising of species, and revision of species complexes or widespread species that are demonstrably non-monophyletic.

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First records and three new species of the family Symphytognathidae (Arachnida, Araneae) from Thailand, and the circumscription of the genus Crassignatha Wunderlich, 1995

ZooKeys ◽

10.3897/zookeys.1012.57047 ◽

2021 ◽

Vol 1012 ◽

pp. 21-53

Author(s):

Francisco Andres Rivera-Quiroz ◽

Booppa Petcharad ◽

Jeremy A. Miller

Keyword(s):

New Species ◽

Dna Sequences ◽

Phylogenetic Analyses ◽

Coastal Region ◽

3D Models ◽

Morphological Data ◽

The North ◽

The Family ◽

Ct Analysis ◽

Three New Species

The family Symphytognathidae is reported from Thailand for the first time. Three new species: Anapistula choojaiaesp. nov., Crassignatha seeliamsp. nov., and Crassignatha seedamsp. nov. are described and illustrated. Distribution is expanded and additional morphological data are reported for Patu shiluensis Lin & Li, 2009. Specimens were collected in Thailand between July and August 2018. The newly described species were found in the north mountainous region of Chiang Mai, and Patu shiluensis was collected in the coastal region of Phuket. DNA sequences are provided for all the species here studied. The relations of these symphytognathid species were tested using previously published phylogenetic analyses on micro orb-weavers. Also, we used micro CT analysis to build 3D models of the male genitalia and somatic characters of two species of Crassignatha Wunderlich, 1995. The molecular phylogeny and 3D models were used to discuss the taxonomy and circumscription of the currently valid symphytognathid genera, with focus on Crassignatha and Patu Marples, 1951. Based on this, three new combinations are suggested: Crassignatha bicorniventris (Lin & Li, 2009), comb. nov., Crassignatha quadriventris (Lin & Li, 2009), comb. nov., and Crassignatha spinathoraxi (Lin & Li, 2009), comb. nov. A new record of Crassignatha danaugirangensisMiller et al. 2014 is reported from Brunei.

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A Novel Parasitoid of Marine Dinoflagellates, Pararosarium dinoexitiosum gen. et sp. nov. (Perkinsozoa, Alveolata), Showing Characteristic Beaded Sporocytes

Frontiers in Microbiology ◽

10.3389/fmicb.2021.748092 ◽

2021 ◽

Vol 12 ◽

Author(s):

Boo Seong Jeon ◽

Myung Gil Park

Keyword(s):

Dna Sequences ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Molecular Data ◽

Small Subunit ◽

New Family ◽

The Family ◽

Marine Dinoflagellates ◽

Developmental Characteristics ◽

Intensive Sampling

The phylum Perkinsozoa is known as an exclusively parasitic group within alveolates and is widely distributed in various aquatic environments from marine to freshwater environments. Nonetheless, their morphology, life cycle, the identity of the host, and physiological characteristics remain still poorly understood. During intensive sampling along the west coast of Korea in October and November 2017, a new parasitoid, which shares several characteristics with the extant families Perkinsidae and Parviluciferaceae, was discovered and three strains of the new parasitoid were successfully established in cultures. Cross-infection experiments showed that among the examined planktonic groups, only dinoflagellates were susceptible to the new parasitoid, with infections observed in species belonging to eight genera. Even though the new parasitoid shared many morphological and developmental characteristics with other Perkinsozoan parasites, it differed from them by its densely packed trophocyte structure without a large vacuole or hyaline material during the growth stage. These characteristics are common among Parviluciferaceae members. Furthermore, through palintomic extracellular sporogenesis, it produced characteristic interconnected sporocytes resembling a string of beads. Phylogenetic analyses based on the small subunit and large subunit ribosomal DNA sequences revealed that the new parasitoid was distantly related to the family Parviluciferaceae and was more closely related to the families Perkinsidae and Xcellidae. Morphological, ultrastructural, and molecular data on the new parasitoid raised the need to erect a new family, i.e., Pararosariidae, within the phylum Perkinsozoa with Pararosarium dinoexitiosum gen. et sp. nov. as the type species. The isolation and establishment in culture of the new parasitoid outside the family Parviluciferaceae in the present study would contribute to the better understanding of the diversity of Perkinsozoan parasites and provide useful material for comparisons to other parasite species in the further study.

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A Phylogenetic Investigation of the New Zealand Pteridaceae Ferns

10.26686/wgtn.16934449.v1 ◽

2021 ◽

Author(s):

◽

Whitney L M Bouma

Keyword(s):

New Zealand ◽

Dna Sequences ◽

Phylogenetic Relationships ◽

Native Species ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Morphological Characters ◽

Taxonomic Confusion ◽

Species Specific

The fern family Pteridaceae is among the largest fern families in New Zealand. It comprises 17 native species among five genera. Traditionally the classification of Pteridaceae was based on morphological characters. The advent of molecular technology, now makes is possible to test these morphology-based classifications. The Pteridaceae has previously been subjected to phylogenetic analyses; however representatives from New Zealand and the South Pacific have never been well represented in these studies. This thesis research aimed to investigate the phylogenetic relationships of the New Zealand Pteridaceae, as well as, the phylogenetic relationships of the New Zealand species to their overseas relatives. The DNA sequences of several Chloroplast loci (e.g. trnL-trnF locus, rps4 and rps4-trnS IGS, atpB, and rbcL) were determined and the phylogenetic relationships of the New Zealand Pteridaceae and several species-specific question within the genus Pellaea and Adiantum were investigated. Results presented in this thesis confirm previously published phylogenetics of the Pteridaceae, which show the resolution of five major clades, i.e.,cryptogrammoids, ceratopteridoids, pteridoids, cheilanthoids, and the adiantoids. The addition of the New Zealand species revealed a possible South West Pacific groups formed by the respective genera, where New Zealand species were generally more related to one another than to overseas relatives. Within the New Zealand Pellaea, the analysis of the trnL-trnF locus sequence data showed that the morphologically-intermediate plants P. aff. falcata, responsible for taxonomic confusion, were more closely related to P. rotundifolia than to P. falcata. Furthermore, the species collected on the Kermadec Islands, previously thought to be P. falcata, are genetically distinct from the Australian P. falcata and they could constitute a new species. Adiantum hispidulum, which is polymorphic for two different hair types being used to distinguish them as different species, was also reinvestigated morphologically and molecularly. Morphological inspection of hairs revealed three hair types as opposed to the previous thought two, and furthermore, they correspond to three different trnL-trnF sequences haplotypes.

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