scholarly journals Effect of Tebuconazole on Growth Processes, Content and Redistribution of Carbohydrates in Horse Bean Seedlings under Scotomorphogenesis

2020 ◽  
Vol 5 (5) ◽  
pp. 332-337
Author(s):  
B. A. Kuts ◽  
◽  
V. G. Kuryata

The article presents a study of changes in the functioning of the source-sink system in the heterotrophic phase of development of horse beans (Vicia faba L.) under the conditions of the artificial combination of external (darkness) factor and internal factor (antigiberelline retardant) during germination. It was found that the action of the drug significantly reduced the length of the epicotyl, root and total seedling. Similarly, the dry matter mass of the seedling organs decreased. The level of using seed spare substances under the influence of retardant decreased, as evidenced by the maximum dry matter of cotyledons in plants of this variant and a decrease in the utilization of reserve substances for root and epicotyl formation during germination. Tebuconazole inhibited the breakdown of starch in the dark. The higher content of sugars in the seeds of scotomorphic plants in comparison with the control is associated with a less intensive outflow for the needs of organogenesis – the formation of root and epicotyl structures. Quantitative changes in nitrogen content in scotomorphic seeds were much smaller than changes in starch content. This indicates that the retardant in the dark inhibits the hydrolysis of the reserve protein of the seed, but the process is started after the hydrolysis of starch. The purpose of the study was to establish the effect of tebuconazole on the processes of germination and redistribution of carbohydrates in seedlings of Vicia faba L. Material and methods. The work was carried out on seedlings of horse beans (Vicia faba L.) of Vivat variety, which is a medium-ripe high-yielding variety with a vegetation period of 100-105 days. The variety is technological, resistant to major diseases, has high resistance to lodging, shedding of beans and their cracking. Results and discussion. The combined effect of darkness and the drug of anti-gibberellin action of tebuconazole were used to regulate the intensity of source-sink relations during the germination of horse bean seeds. The seeds of the experimental variant were soaked for a day in 0.5% solution of the triazole-derived drug tebuconazole. Tebuconazole is a transparent crystalline substance of hazard class 3. The seeds of the control variant were soaked for 24 hours in distilled water and sown in cuvettes with wet sand. The biological replication of the experiments is fivefold. The experiment was performed under the action of light and in dark in order to study the implementation of programs of skoto-and photomorphogenesis. Morpho-biometric parameters (root length, seedling length, dry matter weight of individual organs and the whole plant) were determined on the day of 18 of germination. Determination of nitrogen content was carried out according to Keldal method, starch in the organs of seedlings was performed by iodometric method, to determine the content of total sugar and reducing sugar we used Bertrand method in modification by V. Kosolapov. Conclusion. The combination of exogenous and endogenous factors during seed germination significantly changed the intensity of donor-acceptor relations in horse bean seedlings. Under the action of the drug significantly slowed the growth of seedlings in the dark. There was the decrease in the dry matter of the organs of the seedling in dark and noted lower rates of use of reserve substances for organogenesis during germination

Biologija ◽  
2020 ◽  
Vol 66 (3) ◽  
Author(s):  
Volodymyr Kuryata ◽  
Bohdana Kuts ◽  
Yuriy Prysedsky

Under conditions of a combination of an external (light/dark) factor and a hormonal factor (gibberellic acid) during germination, changes in the functioning of the source-sink system in heterotrophic phase of horse beans development were studied. The increase in the epicotyl, root and seedling length, both in light and dark, as well as in the dry matter of the mass of the seedling organs, was found under drug action. Reserve substances were used more intensively under gibberellin and skotomorphogenesis influence. It is evidenced by the minimum dry matter of cotyledons and higher reserve utilization rates for root and epicotyl formation. Gibberellin stimulated starch breakdown in both, but during germination in dark, the rate of starch use was higher. Other content of sugars in skotomorphic seeds was associated with more intensive outflow for organogenesis needs: formation of root and epicotyl structures. The changes in the content of starch were higher than the changes in nitrogen content in skotomorphic and photomorphic seedlings. This indicates that gibberellin stimulates hydrolysis of reserve protein only after starch hydrolysis in dark. No specific gibberellin regulation of phosphorus and potassium outflow from seeds for organogenesis needs was found, suggesting sufficient mineral nutrients supply to ensure their re-utilization during germination, photo- and skotomorphogenesis processes.


1968 ◽  
Vol 70 (3) ◽  
pp. 299-301 ◽  
Author(s):  
A. Eden

SUMMARYStudies were made of the proximate and mineral compositions of modern strains of both spring· and winter-sown beans. The majority of the winter beans were of the Throws M.S. variety, of the spring beans Minors. On the dry-matter basis spring beans averaged 31·4% crude.protein, winter beans 26·5%—a highly significant difference. The true protein values showed a parallel trend. Winter beans averaged 9% crude fibre, spring beans 8%, again a highly significant difference. The strains confirmed the usually accepted levels of oil and of the principal mineral components, except that modern beans appear to be richer in phosphorus.


Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 169-173 ◽  
Author(s):  
H. M. Kao ◽  
P. B. E. McVetty

Hayman's diallel cross analysis was employed to investigate the nature of the genetic control and heritability of yield, yield components, and phenological and agronomic characters in F1 and F2 generations of spring faba beans (Vicia faba L.). High-yielding S4 inbred lines from five open-pollinated faba bean cultivars were used as parents to generate complete F1 and F2 diallels. The S5 inbred line parents and the 20 cross combinations were planted in randomized complete block experiments with six replications. All characters in the F1 diallel and in the F2 diallel with the exception of days from planting to maturity met all of the assumptions required for Hayman's diallel analysis. Yield, total dry matter, harvest index, and pods per plant exhibited significant apparent overdominance in both the F1 and F2 diallels. It is concluded that substantial immediate increases in yield and total dry matter could be expected from exploiting the apparent overdominant gene action found for these characters in these crosses via F1 hybrids or synthetics. Key words: total dry matter, harvest index, diallel crosses, inheritance, Vicia faba L.


1967 ◽  
Vol 68 (1) ◽  
pp. 69-73 ◽  
Author(s):  
E. A. K. El Saeed

1. Emergence and dry-matter production of largeand small seeds of two varieties of broad beans (Beladi and Rebaya 34) were studied.


Biologija ◽  
2021 ◽  
Vol 67 (1) ◽  
Author(s):  
Volodymyr Kuryata ◽  
Bohdana Kuts ◽  
Yuriy Prysedsky

Functional changes in the source-sink system of maize sprouts under combination of external (light/dark) and hormonal (gibberellic acid/retardant) factors during germination were studied. It was found that the phytohormone action significantly increased the coleoptile, root and seedling length, dry matter mass of organs, both in light and in dark. Tebuconazole caused the opposite effect due to its retardant action. The inactivation of endogenous phytohormone was confirmed by less intensive use of seed substances, the minimum dry matter of root and coleoptile and lower reserve utilization rates under tebuconazole influence, in particular under photomorphogenesis condition. The non-maximum mass of cotyledons may indicate a kind of optimization of use of reserves under tebuconazole treatment. Gibberellin stimulated starch breakdown in both light and dark, but starch usage was higher in dark. The lower content of sugar in the seeds of skotomorphic plants in control and under gibberellin action was explained by intense outflow for the organogenesis needs. Gibberellin stimulated the hydrolysis of reserve protein after intensive starch hydrolysis in dark. Specific gibberellin regulation of phosphorus outflow for the organogenesis processes under the photo- and skotomorphogenesis conditions and no regulation for potassium under photomorphogenesis conditions were found.


1983 ◽  
Vol 63 (2) ◽  
pp. 365-370
Author(s):  
RAYNALD DRAPEAU

The interaction of fertilization methods, ridging and the use of a mulch has been established over a 4-yr period on the yield of a local cultivar of broad bean (Vicia faba L. var. major). No significant effect has been obtained on green pod production, average number of pods per hectare, average number of pods per individual plant (three interrelated parameters), contribution of green grain in percent of the whole green pod, green seed yield and dry matter production of the stubble. Yearly variations have been obtained. The differences in dry stubble production and pod number per plant indicate that broad bean yield seems to be related to the prevailing climatic conditions during plant development and particularly at the blooming stage.Key words: Vicia faba L. var. major, broad bean


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