scholarly journals PENGARUH DOSIS FRUKTOSA TERHADAP INDEKS MASSA TUBUH, PROFIL GLUKOSA DARAH DAN KADAR TRIGLISERID (Studi pada Tikus Wistar yang Diinduksi High Fat Fructose Diet )

Jurnal Gizi ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 53
Author(s):  
- Sunarti 1 ◽  
Fardhiasih Dwi Astuti ◽  
Sufiati Bintanah
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
High Fat ◽  
Glucose Levels ◽  
Food And Beverage ◽  
Male Wistar Rats ◽  
Group 2 ◽  
Simple Carbohydrates

Fructose or often called fruit sugar is one part of simple carbohydrates calledmonosaccharides (Almatsir 2009). Currently, many food and beverage industries usefructose sugar as a sweetener. (Prahstuti 2011). Consumption of high-fructose productsof more than 85 grams per day or more than 25% of caloric needs can lead to increasedtriglyceride levels and insulin resistance (Prahastuti 2011). The purpose of this study wasto evaluate changes in obesity status, fasting blood glucose levels and triglyceride levelsin wistar rats induced with a high fat fructose diet, by giving different doses. Research methods.The design in this study was a Randomized Control Trial(RCT), double bline pre post control design using male wistar rats, aged 6-7 weeksweighing 90-150 grams, totaling 15 individuals divided into 3 groups. Group 1 is a groupof rats fed HFFD with 1% fructose, group 2 is rats fed HFFD with 2% fructose, group 3is rats fed standard feed. Before the treatment, adaptation was carried out for 1 week, afterthat a pre-test was carried out which included measurements of body weight, body length,fasting data glucose levels, and triglyceride levels. The treatment was given for 60 days,in the middle the measurements of body weight, length, fasting data glucose levels, andtriglyceride levels were also carried out. The research location was in the clinicalpathology laboratory, Faculty of Pharmacy, Muhammadiyah University, Surakarta. The results of the study The administration of fructose at a dose of 2% / kg BWincreased fasting blood glucose and triglyceride levels higher than the administration of1%. kgBB. Upgrade has started since day 30 Keywords: Fructose dose, Body Mass Index, fasting blood glucose level, Triglyceride level,Wistar rats, induced High fat fructose diet

scholarly journals HYPOGLYCEMIC EFFECT OF ANDROGRAPHIS ECHIOIDES ON STREPTOZOTOCIN INDUCED EXPERIMENTAL RATS

2015 ◽  
Vol 7 ◽  
pp. 55
Author(s):  
Mani Rupeshkumar ◽  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Hypoglycemic Effect ◽  
Reference Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Single Intraperitoneal Injection

The present study aims to study the hypoglycemic effect of methanol extract of Andrographisechioides (MEAE) in streptozotocin (STZ)-induced diabetic Wistar rats. Hyperglycemia was induced in rats by single intraperitoneal injection of STZ (55 mg/kg bodyweight). Three days after STZ induction, the hyperglycemic rats were treated with MEAE orally at the doses of 200, 500, and 800 mg/kg body weight daily for 21 days. Glibenclamide (1 mg/kg, orally) was used as reference drug. The fasting blood glucose levels were measured on each 7th day during the 21 days of treatment.

scholarly journals Analysis on Soy-Yamgurt Potential as Antidiabetic in Streptozotocin-Nicotinamide Induced Rats

2018 ◽  
Vol 1 (1) ◽  
pp. 58-70
Author(s):  
Jessica Kwanariesta ◽  
Herla Rusmarilin ◽  
Ismed Suhaidi
Keyword(s):  
Body Weight ◽  
Lactic Acid ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Average Weight ◽  
In Vivo Test ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Male Wistar Rats

This study was conducted to determine the potential of soy-yamgurt probiotics drink from the mixture of yam and soy extract with a ratio of 1: 1 in reducing the fasting blood glucose level of rats induced by streptozotocin-nicotinamide. Based on the analysis of chemical and phytochemical characteristics of soy-yamgurt, it was found that it has water content (80.97%), total solids (18.85%), protein content (11.95%), total lactic acid (1.03%), viscosity (20.56 Pa.s), lactic acid bacteria (10.22 log CFU/g), fiber content (0.98%), IC50 value (44.99 μg/ml), total phenol (711.90 μgGAE/g) and total flavonoids (1345.73 μgQE/g). The in vivo test used thirty 10-16 week old male Wistar rats with an average weight of 160-200 g divided into 5 groups: P1 (control), P2 (streptozotocin-nicotinamide), P3 (glibenclamide of 0.45 mg/kg body weight), P4 (3 ml of soy-yamgurt) and P5 (3.5 ml of soy-yamgurt).The results showed that the soy-yamgurt at the doses of 3 ml/160 g body weight and 3.5 ml/160 g body weight for 4 weeks in streptozotocin-nicotinamide-induced rats had significantly (P<0.01) lowered blood glucose levels and increased their weight.

scholarly journals THE EFFECT OF SOURSOP LEAF EXTRACT ON PANCREATIC BETA CELL COUNT AND FASTING BLOOD GLUCOSE IN MALE WISTAR RATS EXPOSED TO A HIGH-FAT DIET AND STREPTOZOTOCIN

2017 ◽  
Vol 53 (1) ◽  
pp. 12 ◽  
Author(s):  
Dewa Ayu Agung Alit Suka Astini ◽  
H Ari Gunawan ◽  
R Mochamad Wirono Aman Santoso ◽  
Susilowati Andajani ◽  
Ahmad Basori
Keyword(s):  
Blood Glucose ◽  
Beta Cell ◽  
Beta Cells ◽  
High Fat Diet ◽  
Pancreatic Beta Cells ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Fasting Blood Glucose ◽  
High Fat ◽  
Male Wistar Rats

Based on some researches known that soursop leaf extract can improve beta cell injury. The aims of this study was to analyze the effect of soursop leaf extract on fasting blood glucose (FBG) and pancreatic beta cell number in male Wistar rats wich were exposed to a high-fat diet and streptozotocin. This study design is the only randomized posttest control group design. The total sample size is 50 male Wistar rats. The independent variable: high-fat diet, STZ, and soursop leaf extract; the dependent variable: pancreatic beta cells number, and FBG3. Data tested for normality with Kolmogorov-Smirnov (a=0.05) and tested of homogeneity with Levene (a =0.05). Comparison test between groups with Kruskal-Wallis (a=0.05), followed by Mann Whitney. Correlation test with Pearson (a=0.05) between dose of the soursop leaf extract and FBG3, and between dose and the number of pancreatic beta cells. The results of this study showed that the soursop leaf extract at a dose of 100 mg/kg and 150 mg/kg have an effect on fasting blood glucose levels and panreatic beta cells number;2)There is a significant negative correlation between the orograstric lavage of soursop leaf extract with FBG3 (r=-0.647;p<0.001), the increasing doses of soursop leaf extract, further lowering fasting blood glucose levels;3)There is a significant positive correlation between the orograstric lavage of soursop leaf extract with the number of pancreatic beta cells (r=0,759;p<0,001), the increasing doses of soursop leaf extract, further increasing pancreatic beta cells number. In conclusion, increasing doses of soursop leaf extract, further lowering fasting blood glucose and increasing the number of pancreatic beta cells.

scholarly journals Potency of Okra flour (Abelmoschus esculentus) in improving adiponectin level and total antioxidant capacity of high fat diet streptozotocin rat model

10.5219/1136 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 644-650 ◽  
Author(s):  
Ahdiyatul Fauza ◽  
Ahmad Ni'matullah Al-Baarri ◽  
Kis Djamiatun
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
High Fat Diet ◽  
Total Antioxidant Capacity ◽  
Fasting Blood Glucose ◽  
High Fat ◽  
Post Intervention ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Total Antioxidant

T2DM has increase in global-morbidity and mortality. Oxidative stress and adiponectin-levels are important for insulin-resistance and pancreatic-b-cell-dysfunction in T2DM. Okra fruit is rich of quercetin and phytosterol which have positive-effect for T2DM. Research aimed was to study the effect of okra-flour to adiponectin-levels and total-antioxidant-capacity (TAC) in T2DM. Thirty Wistar-rats were divided randomly in five groups. K1 and (X1, X2 and X3)-treated-groups were in T2DM-condition-induced by high-fat-diet-(HFD)-Streptozotochin-(STZ)-nicotinamid-(NA). Healthy-controls-(K2)-group was also used. Okra-flour was given orally for 28 days at doses of 0.1; 0.2 and 0.3 g/Kg-body-weight/d to X1, X2 and X3-groups, respectively. Statistical program was used to analyse the different between pre-post-intervention, and between groups. Correlations between variables were also analysed. The serum-adiponectin and TAC-levels were measured by ELISA and ABTS-methods, respectively. By comparing pre and post-intervention, adiponectin levels of all-intervention-(X1, X2, X3)-group were  increase (p = 0.027 for X1 and X2; p = 0.028 for X3), while in the same period the decrease were found in group K1 (p = 0.026) and K2 (p = 0.028). Increase-TAC-levels pre-post-intervention was observed in group all-intervention-groups (p = 0.027), while no change in K1 (p = 0.66) and the decrease in group K2 (p = 0.039). Reduce-fasting-blood-glucose-levels pre-post-intervention were shown in the all-intervention-groups (p = 0.028), while for the K1 groups was increase (p = 0.028). There were significant differences between the five-groups on fasting-blood-glucose-levels, adiponectin and TAC-levels, and X3-group showed the highest adiponectin and TAC-levels. Very-strong-correlations were found between glucose-adiponectin-TAC-levels-post-intervention. Okra-flour make better glucose-adiponectin and TAC-levels in T2DM-conditions. Okra dose of 0.30 g/Kg-body-weight/day is the best in increasing adiponectin and TAC-levels.

scholarly journals The Hypoglycaemic, Antihyperlipidemic and Antioxidative Effects of Anacardium Occidentale Methanolic Nut Extract in Streptozotocin-Induced Diabetic Male Wistar Rats

2021 ◽  
Author(s):  
Folasade O. AJAO ◽  
Michael A. Olamoyegun ◽  
Marcus O. Iyedupe
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Wistar Rats ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Anacardium Occidentale ◽  
Low Density ◽  
Reference Drug ◽  
Antioxidative Effects ◽  
Group 2

Abstract Background: This research work investigated the antidiabetic, anti-hyperlipidemic, and antioxidative effects of Anacardium occidentale methanolic nut extract in Streptozotocin (STZ)-induced diabetic Wistar rats. Methods: Forty (40) Wistar rats weighing 250±30g were randomly divided into five groups of 8 rats each. Group 1 served as the control; Group 2-5 were induced with diabetes with a single dose of 50mg/kg bw of streptozotocin intraperitoneally. After diabetes induction, Group 2 served as the STZ-only group, Groups 3 and 4 were administered 100mg/kg bw and 200mg/kg bw p.o Anacardium occidentale nut extract, respectively, while Group 5 was administered 2mg/kg bw of glimepiride as a reference drug for a period of 4 weeks. Food and water intake were monitored daily, body weight, and blood glucose levels weekly throughout the experiment. On day 29, the animals were sacrificed, and blood samples were collected through cardiac puncture for biochemical studies. Results: Administration of the nut extract significantly (p<0.05) increased the food intake and body weight of diabetic treated rats, fasting blood glucose level and oral glucose tolerance test (OGTT) decreased significantly (p<0.05) in treated rats. On lipid profile, administration of nut extract significantly decreased (p<0.05) triglyceride, low-density lipoprotein, total cholesterol, and very-low-density lipoprotein concentrations while it significantly increases (p<0.05) the high-density lipoprotein. Anacardium occidentale nut extract caused significantly (p<0.05) increases in SOD, GPx, GSH, and CAT levels with a decrease MDA level in diabetic treated rats. Markers of liver and kidney functions were also improved in diabetic treated rats.Conclusion: Treatment with Anacardium occidentale methanolic nut extract has hypoglycemic, hypolipidemic, and hepato-protective effects in diabetic rats. It also alleviates oxidative stress activity and restoration of markers of kidney function, and may be useful as alternative therapy in the management of diabetes and its related complications.

Abstract 404: NLR Family, Pyrin Domain-containing 3 Knockout Rescues Cardiac Dysfunction Induced by High-fat Diet Feeding

2017 ◽  
Vol 121 (suppl_1) ◽  
Author(s):  
Matthew R Peterson ◽  
Samantha Haller ◽  
Tracy Ta ◽  
Luiza Bosch ◽  
Aspen Smith ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Inflammatory Response ◽  
Glucose Tolerance ◽  
High Fat Diet ◽  
Fasting Blood Glucose ◽  
Left Ventricular ◽  
Normal Diet ◽  
High Fat ◽  
Pyrin Domain

NLR family, pyrin domain-containing 3 (NLRP3) is a pattern recognition receptor responsible for perpetuating an inflammatory response through production of pro-inflammatory cytokines IL-1β and IL-18. It has been implicated in the sustained inflammatory response in obesity and multiple cardiovascular disease conditions. In order to investigate NLRP3 as a potential therapeutic target in metabolic syndrome, C57BL/6 wild-type (WT) and NLRP3 knockout (NLRP3-\-) mice were fed a normal diet (ND; 12% fat chow) or a high fat diet (HFD; 45% fat chow) for 5 months. At 5 months, echocardiography and glucose tolerance tests (GTTs) were performed. Cardiac function assessed by fractional shortening (FS) was significantly impaired by HFD feeding in the WT group (0.335 HFD vs. 0.456 ND; p<0.05) but not in the NLRP3-\- (0.449 HFD vs. 0.492 ND; p>0.05). FS was higher in NLRP3-\-HFD than in WT-HFD (p<0.05). Two-dimensional analysis shows the FS difference between NLRP3-\-HFD and WT-HFD was primarily explained by the difference in left ventricular end-systolic dimension (0.2716 cm WT vs. 0.1883 cm NLRP3-\-; p<0.05). Glucose tolerance measured by area under the curve (AUC) was significantly impaired by HFD feeding for both WT (23183 ND vs. 57298 HFD; p<0.001) and NLRP3-\- (23197 ND vs. 44626 HFD; p<0.001), but significantly better in the NLRP3-\-HFD than in WT-HFD (p<0.01). HFD feeding increased fasting blood glucose (FBG) for both WT (97.7 mg . dl -1 ND vs. 164.7 mg . dl -1 HFD; p<0.01) and NLRP3-\- (80.50 mg . dl -1 ND vs. 108.8 mg . dl -1 HFD; p<0.05), but significantly less in NLRP3-\- mice (NLRP3-\- vs. WT; p<0.05). For GTTs, body weight was significantly higher in the WT than NLRP3-\- fed HFD (47.93 g vs. 36.5 g; p<0.001). Body weight explained 92% of variation in glucose tolerance (p<0.0001) and 69% of variation in fasting blood glucose (p<0.0001). WT-HFD averaged 1.31X heavier than NLRP3-\-HFD, while the AUC for the IGTT was 1.28X larger for the WT-HFD than NLRP3-\-HFD. Body weights were not significantly different between genotypes at the time of echo. The results suggest that knockout of NLRP3 may be protective against HFD induced cardiovascular dysfunction. A protective effect on glucose tolerance is not strongly supported.

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