AMELIORATIVE EFFECTS OF GRAPE SEED OIL ON CHROMIUM-INDUCED NEPHROTOXICITY AND OXIDATIVESTRESS IN RATS

The current study focused on investigating the renoprotective effects of grape seed oil (GSO) against hexavalent chromium (Cr (VI))-induced nephrotoxicity. A total of 40 male rats were randomly divided into four groups: group I served as the control group, group II received 1000 mg/L potassium dichromate (353.5 mg/L Cr(VI)) in drinking water for 12 weeks, group III received 3.7 g/kg body weight/day GSO orally for 12 weeks, and group IV received GSO together with potassium dichromate for 12 weeks. Cr(VI) significantly increased serum levels of urea, creatinine, potassium and glucose. In addition, Cr(VI) increased MDA levels and induced renal tissue damage and DNA damage. On the other hand, Cr(VI) decreased serum levels of sodium and antioxidant defence system [reduced glutathione (GSH) and catalase (CAT)]. However, treatment with GSO prevented elevation levels of serum urea, creatinine, potassium and glucose. In addition, GSO enhanced sodium level, renal tissue antioxidant defense system due to its curative effect ameliorated particularly oxidative stress, renal tissue and DNA damage. In conclusion, these results demonstrate that GSO is a promising nephroprotective agent against Cr(VI)-induced nephrotoxicity.Key words: grape seed oil; hexavalent chromium; nephrotoxicity; DNA damage BLAŽILNI UČINKI OLJA GROZDNIH PEŠK PRI TOKSIČNI OBREMENITVI LEDVIC TER VPLIV NA OKSIDATIVNI STRES PODGAN, POVZROČEN S KROMOM Povzetek: Študija je bila osredotočena na proučevanje zaščitnih učinkov olja grozdnih pešk (GSO) pri toksični obremenitvi ledvic, povzročeni s heksavalentnim kromom (Cr (VI)). Štirideset samcev podgan je bilo naključno razdeljenih v štiri skupine: skupina I - kontrolna skupina, skupina II, ki je v pitni vodi 12 tednov prejemala 1000 mg/L kalijevega dikromata (353,5 mg/L Cr (VI)), skupina III, ki je peroralno 12 tednov prejemala 3,7 g/kg telesne mase/dan GSO ter skupina IV, ki je 12 tednov prejemala GSO skupaj s kalijevim dikromatom. Cr(VI) je znatno zvišal serumske ravni sečnine, kreatinina, kalija in glukoze v serumu. Poleg tega je Cr(VI) zvišal raven MDA in povzročil poškodbe ledvičnega tkiva in poškodbe DNK. Po drugi strani je Cr(VI) znižal serumsko raven natrija in antioksidativnega obrambnega sistema, zmanjšal raven glutationske peroksidaze in katalaze. Dodajanje GSO poskusnim živalim je preprečilo zvišanje ravni sečnine v serumu, kreatinina, kalija, natrija in glukoze. Poleg tega je GSO izboljšal obrambni sistem antioksidantov ledvičnega tkiva. Zaradi svojega zdravilnega učinka je izboljšal zlasti oksidativni stres, poškodbe ledvičnega tkiva in DNK. Rezultati kažejo, da je GSO obetavno zaščitno sredstvo za ledvica pri toksični obremenitvi, povzročeni s Cr(VI).Ključne besede: olje grozdnih pešk; heksavalentni krom; nefrotoksičnost; poškodba DNK

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Grape Seed Oil as a Natural Therapy in Male Rats with Alzheimer’s Diseases

Advanced Pharmaceutical Bulletin ◽

10.34172/apb.2020.052 ◽

2020 ◽

Vol 10 (3) ◽

pp. 430-436

Author(s):

Farnoosh Berahmand ◽

Golnoush Anoush ◽

Mir-Jamal Hosseini ◽

Mahdieh Anoush

Keyword(s):

Spatial Memory ◽

Seed Oil ◽

Neurodegenerative Disorder ◽

Memory Performance ◽

Grape Seed ◽

Treated Group ◽

Post Treatment ◽

Male Rats ◽

Grape Seed Oil ◽

Male Wistar Rats

Purpose: Alzheimer’s disease (AD) is a chronic neurodegenerative disorder, with an increasing prevalence rate, mostly related to cholinergic system. According to the difficulties and complications in management of AD, this study was carried out to evaluate the efficacy of grape seed oil (GSO) on scopolamine (Scop) induced Alzheimer’s in male rats. Methods: 64 healthy male Wistar rats received different treatments such as: normal saline (NS), donepezil (Don), Scop and GSO, according to the previously designed protocol. Morris (MWM) was applied for spatial memory tests. Right after the behavioral tests, the brains were removed and the hippocampus was separated for evaluation of acetylcholine levels as well as cell death and neuro inflammation. Results: The results of the test day indicated that the mean Q2 time was increased in both GSO test groups (P<0.05) and Don treated group (P<0.001).The spectrophotometric findings affirm that both GSO co-treatment and post-treatment were effective in augmenting brain acetylcholine levels (P<0.01 and P<0.05 respectively). The microscopic findings of Hamp;E dyed tissues confirmed the above mentioned results for different treatments except for GSO post treatment, in which the viability of cells were very low. Conclusion: The results implied that supplementation of rats with GSO caused a significant augmentation in spatial memory performance as well as acetylcholine levels and cell viability in the presence of Scop. This effect was comparable to that of Don especially when GSO was used as co-treatment.

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The protective effects of grape seed oil on induced osteoarthritis of the knee in male rat models

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-020-01932-y ◽

2020 ◽

Vol 15 (1) ◽

Author(s):

Nader Tanideh ◽

Soheil Ashkani-Esfahani ◽

Farid Sadeghi ◽

Omid Koohi-Hosseinabadi ◽

Cambyz Irajie ◽

...

Keyword(s):

Seed Oil ◽

Joint Space Width ◽

Sodium Hyaluronate ◽

Grape Seed ◽

Male Rats ◽

Joint Surface ◽

Male Rat ◽

Protective Effects ◽

Rat Models ◽

Grape Seed Oil

Abstract Background Osteoarthritis (OA), though being treated via various methods and medicines, is still a major healthcare concern mostly due to the increase in diagnosis of these age-related diseases. The present study aimed at investigating the effects of oral and intra articular injection of grape seed oil on OA in male rat models. Methods and materials Seventy male rats were selected and their anterior cruciate ligament was cut to induce OA. They were divided into 7 groups (n = 10): C1, no treatment; C2, receiving 300 mg/day of Piascledine per os (PO); C3, 1 mg sodium hyaluronate intra-articularly in days 1, 7, 14; C4, 1 mg methyl-prednisolone acetate intra-articularly; E1, avocado and grape seed oil combination (2:1, 300 mg/day) PO; E2, 500 mg/day of grape seed oil PO; E3, 200 mg/day grape seed oil intra-articularly. After 10 weeks, the rats were anesthetized and evaluated radiologically and histopathologically. P value ≤ 0.05 was considered as statistically significant. Results All the groups made significant differences with C1 regarding all inspected radiological criteria (P ≤ 0.05). E1 and E3 showed significantly better effects on medial femoral condyle, medial tibial condyle, joint space width, total osteophyte, and OA scores (P ≤ 0.04). Joint surface, matrix, cell distribution, cell population viability, calcification, and subchondral bone in treatment groups had significantly better scores versus C1 (P ≤ 0.04). E1 and E3 had significantly superior results regarding joint surface, cell viability, and calcification (P ≤ 0.04). Conclusions Grape seed oil has protective effects, both in injectable form and PO in combination with avocado, on OA in rats. Further clinical trials are necessary.

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Possible action of grape seed oil on brain toxicity induced by methomyl or imidacloprid of male rats

Journal of Scientific Research in Science ◽

10.21608/jsrs.2018.25659 ◽

2018 ◽

Vol 35 (1) ◽

pp. 250-272

Author(s):

Doaa Moeen ◽

Afnan Amer ◽

Nadia Ismail ◽

Elham Ali

Keyword(s):

Seed Oil ◽

Grape Seed ◽

Male Rats ◽

Grape Seed Oil

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Possible action of grape seed oil on brain toxicity induced by methomyl or imidacloprid of male rats.

Journal of Scientific Research in Science ◽

10.21608/jsrs.2018.12365 ◽

2018 ◽

Vol 35 (1) ◽

pp. 128-149 ◽

Cited By ~ 1

Author(s):

Afnan Amer ◽

Nadia Ismail ◽

Elham Ali ◽

Doaa Moeen

Keyword(s):

Seed Oil ◽

Grape Seed ◽

Male Rats ◽

Grape Seed Oil

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Study of antidepressant effects of grape seed oil in male mice using tail suspension and forced swim tests

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v12i4.33520 ◽

2017 ◽

Vol 12 (4) ◽

pp. 397 ◽

Cited By ~ 4

Author(s):

Zahra Rabiei ◽

Sajad Naderi ◽

Mahmoud Rafieian-Kopaei

Keyword(s):

Chronic Stress ◽

Normal Saline ◽

Seed Oil ◽

Grape Seed ◽

Male Mice ◽

Tail Suspension ◽

Forced Swim ◽

Group I ◽

Antidepressant Effects ◽

Grape Seed Oil

The aim of this study was to investigate the antidepressant effects of grape seed oil in male mice (n=60) using tail suspension and forced swim tests. Mice were divided to six groups of 10 in each group. Group I: Intraperitoneally receiving normal saline; Group II: Received normal saline & stress; Group III-V: Injected (i.p.) with different (60, 120, and 240 mg/kg) doses of grape seed oil; and Group VI: Received fluoxetine (10 mg/kg). Grape seed oil in 60 mg/kg significantly decreased immobility time in tail suspension and forced swim tests under chronic unpredictable stress compared to groups under chronic stress receiving normal saline and grape seed oil (120 and 240 mg/kg). Grape seed oil (60 mg/kg) significantly improved the motor balance in mice under chronic stress. It had no significant effect on serum corticosterone level. Grape seed oil can improve depression symptoms in mice.Video Clip of Methodology:0 min 14 sec <a href="https://youtube.com/v/7JgyR4mTUOw">Full Screen</a> <a href="https://youtube.com/watch?v=7JgyR4mTUOw">Alternate</a>

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Possible action of grape seed oil on brain toxicity induced by methomyl or imidacloprid of male rats.

Journal of Scientific Research in Science ◽

10.21608/jsrs.2018.25535 ◽

2018 ◽

Vol 35 (1) ◽

pp. 250-272

Author(s):

Doaa Moeen ◽

Afnan Amer ◽

Nadia Ismail ◽

Elham Ali

Keyword(s):

Seed Oil ◽

Grape Seed ◽

Male Rats ◽

Grape Seed Oil

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Effects of virgin olive oil and grape seed oil on physicochemical and antimicrobial properties of pectin-gelatin blend emulsified films

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2021.01.020 ◽

2021 ◽

Author(s):

Mehran Dovlat Khah ◽

Babak Ghanbarzadeh ◽

Leila Rufegari Nezhad ◽

Alireza Ostadrahimi

Keyword(s):

Olive Oil ◽

Seed Oil ◽

Virgin Olive Oil ◽

Antimicrobial Properties ◽

Grape Seed ◽

Grape Seed Oil

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Characterization of fatty acid, antioxidant, and polyphenol content of grape seed oil from different Vitis vinifera L. varieties

OCL ◽

10.1051/ocl/2021017 ◽

2021 ◽

Vol 28 ◽

pp. 30

Author(s):

Viktória Kapcsándi ◽

Erika Hanczné Lakatos ◽

Beatrix Sik ◽

László Ádám Linka ◽

Rita Székelyhidi

Keyword(s):

Seed Oil ◽

Grape Seed ◽

Seed Oils ◽

Grape Seeds ◽

Polyphenol Content ◽

Total Polyphenol ◽

Total Polyphenol Content ◽

Grape Seed Oil ◽

Total Antioxidant ◽

Grape Varieties

In this study, we examined the yield and oil quality of eight different grape varieties. For the experiments, the grape seeds were obtained from the Benedictine Pannonhalma Archabbey in the northwestern region of Hungary. The aim of the studies was to determine the oil yield obtained by extraction and to examine the differences between the fatty acid composition, antioxidant capacity, and total polyphenol content of the oils of different grape varieties. Based on the results, the oil content of the grape seeds varied between 99.91 g/kg and 126.74 g/kg. The grape seed oils analysed contained significant amounts of stearic acid (3.42–9.93%), palmitic acid (7.81–10.66%), oleic acid (14.29–19.92%), and linoleic acid (66.85–72.47%). Besides, the grape seed oils tested contained several other fatty acids in small amounts. There were significant differences in the total antioxidant and total polyphenol content of the oils. Total polyphenol content ranged from 0.24 to 1.13 mg GAE/g, while the total antioxidant content changed between 0.12 and 0.78 μg TEAC/g. The results show that the studied varieties are suitable for the production of table grape seed oil based on their oil yield, and the oils have favourable, health-protecting properties in terms of their quality.

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