Immunohistochemical analysis of abscisic acid (ABA), indolylacetic acid(IAA) and HvPIP2 aquaporins in the embryos of an ABA-deficient barley mutant and its original variety during seed germination

In this work we investigated whether priming with auxin, cytokinin, gibberellin, abscisic acid and ethylene, alters the physiological responses of seeds of pigeon pea germinated under water and cadmium stress. Seeds treated with water or non-treated seeds were used as control. Although compared to non-treated seeds we found that the hormone treatments improve the germination of pigeon pea under cadmium stress, however, these treatments did not differ from water. However, we also observed a trend of tolerance to the effects of cadmium in the presence of ethylene, suggesting that the use of this hormone may be an efficient method to overcome seed germination under metal stress.

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Seed Dormancy: The Complex Process Regulated by Abscisic Acid, Gibberellins, and Other Phytohormones that Makes Seed Germination Work

Phytohormones - Signaling Mechanisms and Crosstalk in Plant Development and Stress Responses ◽

10.5772/intechopen.68735 ◽

2017 ◽

Cited By ~ 5

Author(s):

Anna Skubacz ◽

Agata Daszkowska‐Golec

Keyword(s):

Abscisic Acid ◽

Seed Germination ◽

Seed Dormancy ◽

Complex Process

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Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 inhibit WRKY75 function in abscisic acid-mediated leaf senescence and seed germination

Journal of Experimental Botany ◽

10.1093/jxb/erab391 ◽

2021 ◽

Cited By ~ 1

Author(s):

Haiyan Zhang ◽

Liping Zhang ◽

Yunrui Ji ◽

Yifen Jing ◽

Lanxin Li ◽

...

Keyword(s):

Abscisic Acid ◽

Seed Germination ◽

Leaf Senescence ◽

Chromatin Immunoprecipitation ◽

Sigma Factor ◽

Dependent Manner ◽

Negative Regulators ◽

Factor Binding ◽

Physiological Processes ◽

Increased Sensitivity

Abstract The plant-specific VQ gene family participates in diverse physiological processes but little information is available on their role in leaf senescence. Here, we show that the VQ motif-containing proteins, Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 are negative regulators of abscisic acid (ABA)-mediated leaf senescence. Loss of SIB1 and SIB2 function resulted in increased sensitivity of ABA-induced leaf senescence. In contrast, overexpression of SIB1 significantly delayed this process. Moreover, biochemical studies revealed that SIBs interact with WRKY75 transcription factor. Loss of WRKY75 function decreased sensitivity to ABA-induced leaf senescence, while overexpression of WRKY75 significantly accelerated this process. Chromatin immunoprecipitation assays revealed that WRKY75 directly binds to the promoters of GOLDEN 2-LIKE1(GLK1) and GLK2, to repress their expression. SIBs repress the transcriptional function of WRKY75 and negatively regulate ABA-induced leaf senescence in a WRKY75-dependent manner. In contrast, WRKY75 positively modulates ABA-mediated leaf senescence in a GLK-dependent manner. In addition, SIBs inhibit WRKY75 function in ABA-mediated seed germination. These results demonstrate that SIBs can form a complex with WRKY75 to regulate ABA-mediated leaf senescence and seed germination.

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