Bedbugs are harmful human blood-sucking ectoparasites. The disadvantages of a bedbug bite cause itching, redness bumps, secondary infections, and anemia in children. Bedbugs controller so far uses harmful chemical pesticides, but reappearance of bedbugs indicates less effective chemical pesticides, and becomes a serious problem. So it needs safer controller for human health. This study aims to analyze the d-Limonene activity of orange peel (Citrus sinensis L.) in controlling the bedbug infestation (Cimex cimicidae). The study was conducted through 5 stages: d-Limonene isolation, GC-MS characterization, bioassay Y test d-Limonene nanoparticles, SEM characterization, and d-Limonene activity test on bedbug. The results showed that the essential oil of orange peel contained the main compounds as d-Limonene (90.018%), 1,6-Octadien-3-ol, 3,7-dimethyl- (3,017%), β-Myrcene (2.071%), Octanal (0.349%), 1-Octanol (0.589%), 1R-α-Pinene (0.688%), 6-Octen-1-ol, 3,7-dimethyl- (0.667%), 3-Cyclohexene-1- methanol, α, α, 4-trimethyl-, (S) - (1.453%), and Bicyclo [3.1.1] heptane, 6.6-dimethyl-2-methylene-, (1S) - (1.147%). The Bioassay Y test shows that d-Limonene was both repellent and fumigant, d-Limonene nanoparticles had uneven and irregular surface morphology, and in the flea activity test showed more than 50% bedbugs death. These research concluded that d-Limonene compounds from sweet orange peel had activity to control the bedbugs.
Safety and efficacy of feed additives consisting of expressed sweet orange peel oil and its fractions from Citrus sinensis (L.) Osbeck for use in all animal species (FEFANA asbl)
