scholarly journals Isolasi Bakteri Selulolitik dari Limbah Kulit Pisang

2018 ◽  
Vol 2 (1) ◽  
pp. 46-52
Author(s):  
Sukmawati Sukmawati
Keyword(s):  
Carboxymethyl Cellulose ◽  
Banana Peel ◽  
Index Test ◽  
Cellulolytic Bacteria ◽  
Purification Method ◽  
Isolation And Purification ◽  
Selective Media ◽  
Morphology Characteristic ◽  
Industry Sector ◽  
West Papua

Cellulose can be found in whole parts of plants.  Cellulose can be degraded by cellulose enzyme. Cellulose enzyme is produced by cellulolytic bacteria which has ability to degrade the cellulose and fiber. Cellulose enzyme is extremely important in industry sector. This research aims to isolate the cellulosic bacteria of peel waste of Banana in Sorong, West Papua. This research used isolation and purification method by using 1 gram of Banana peel. It was isolated in CMC (Carboxymethyl cellulose) selective media and morphology characteristic test using 400X-1000X microscope and zone index test. The result showed that the cellulosic ability was found in isolate B with pure zone index 3mm and the smallest pure zone index was in isolate H with pure zone index 0,8 mm. while, the isolate C had zone pure index 1,5 mm, isolate D 1,625 mm, isolate E 1 mm, isolate F 0,714mm, and isolate G 2 mm. 

scholarly journals Green Route for the Isolation and Purification of Hyrdoxytyrosol, Tyrosol, Oleacein and Oleocanthal from Extra Virgin Olive Oil

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3654
Author(s):  
Antonio Francioso ◽  
Rodolfo Federico ◽  
Anna Maggiore ◽  
Mario Fontana ◽  
Alberto Boffi ◽  
...  
Keyword(s):  
Olive Oil ◽  
High Performance ◽  
Virgin Olive Oil ◽  
Deep Eutectic Solvent ◽  
Extra Virgin Olive Oil ◽  
Hazardous Substances ◽  
Purification Method ◽  
Total Recovery ◽  
Isolation And Purification ◽  
Natural Deep Eutectic Solvent

Extra virgin olive oil (EVOO) phenols represent a significant part of the intake of antioxidants and bioactive compounds in the Mediterranean diet. In particular, hydroxytyrosol (HTyr), tyrosol (Tyr), and the secoiridoids oleacein and oleocanthal play central roles as anti-inflammatory, neuro-protective and anti-cancer agents. These compounds cannot be easily obtained via chemical synthesis, and their isolation and purification from EVOO is cumbersome. Indeed, both processes involve the use of large volumes of organic solvents, hazardous reagents and several chromatographic steps. In this work we propose a novel optimized procedure for the green extraction, isolation and purification of HTyr, Tyr, oleacein and oleocanthal directly from EVOO, by using a Natural Deep Eutectic Solvent (NaDES) as an extracting phase, coupled with preparative high-performance liquid chromatography. This purification method allows the total recovery of the four components as single pure compounds directly from EVOO, in a rapid, economic and ecologically sustainable way, which utilizes biocompatible reagents and strongly limits the use or generation of hazardous substances.

scholarly journals An integrated cell isolation and purification method for rat dorsal root ganglion neurons

2019 ◽  
Vol 47 (7) ◽  
pp. 3253-3260
Author(s):  
Huaishuang Shen ◽  
Minfeng Gan ◽  
Huilin Yang ◽  
Jun Zou
Keyword(s):  
Neuropathic Pain ◽  
Dorsal Root Ganglion ◽  
Primary Culture ◽  
Dorsal Root ◽  
Cell Isolation ◽  
Dorsal Root Ganglion Neurons ◽  
Drg Neurons ◽  
Purification Method ◽  
Isolation And Purification ◽  
Ganglion Neurons

Objective Neurobiology studies are increasingly focused on the dorsal root ganglion (DRG), which plays an important role in neuropathic pain. Existing DRG neuron primary culture methods have considerable limitations, including challenging cell isolation and poor cell yield, which cause difficulty in signaling pathway studies. The present study aimed to establish an integrated primary culture method for DRG neurons. Methods DRGs were obtained from fetal rats by microdissection, and then dissociated with trypsin. The dissociated neurons were treated with 5-fluorouracil to promote growth of neurons from the isolated cells. Then, reverse transcription polymerase chain reaction and immunofluorescence assays were used to identify and purify DRG neurons. Results Isolated DRGs were successfully dissociated and showed robust growth as individual DRG neurons in neurobasal medium. Both mRNA and protein assays confirmed that DRG neurons expressed neurofilament-200 and neuron-specific enolase. Conclusions Highly purified, stable DRG neurons could be easily harvested and grown for extended periods by using this integrated cell isolation and purification method, which may help to elucidate the mechanisms underlying neuropathic pain.

scholarly journals The bacterial population adherent to plant particles in the rumen of reindeer fed lichen, timothy hay or silage

Rangifer ◽  
1998 ◽  
Vol 18 (2) ◽  
pp. 55 ◽  
Author(s):  
Monica Alterskjær Olsen ◽  
Svein Disch Mathiesen
Keyword(s):  
Carboxymethyl Cellulose ◽  
Rangifer Tarandus ◽  
Bacterial Population ◽  
Anaerobic Bacteria ◽  
Negative Influence ◽  
Cellulolytic Bacteria ◽  
Bacterial Strains ◽  
Butyrivibrio Fibrisolvens ◽  
Rangifer Tarandus Tarandus ◽  
Wet Weight

Male reindeer (Rangifer tarandus tarandus) calves taken from a natural winter pasture were given ad lib. access to lichen (n = 3), timothy silage (n = 3) and hay (n = 3) for 7 weeks. Median numbers of viable anaerobic bacteria adherent to the plant particles (cells/g wet weight of rumen solids), growing on a habitat simulating medium (M8V), were significantly higher (P = 0.05) in the rumen of reindeer fed lichen (26.5 x 109- 53.0 x 109) and hay (4.0 x 109- 40.5 x 109), compared to reindeer fed silage (1.15 x 109 - 3.25 x 109). Anaerobic bacterial strains (n = 551) from the plant particles obtained from the rumen of the nine reindeer examined, were isolated using an acid swollen cellulose medium (M8SC) and tested for their ability to hydrolyse carboxymethyl cellulose (CMC). The proportion of CMC hydrolysing adherent bacteria isolated from M8SC was significantly higher in reindeer fed hay (21.5%) compared ro animals fed lichen (5.3%) and silage (2.7%) (P = 0.05). The CMC hydrolysing bacterial srrains (n=42) isolated from reindeer fed hay where characterised as non-cellulolytic Butyrivibrio fibrisolvens (9.5%), cellulolytic B. fibrisolvens (50.0%), Clostridium sp. (2.4%) and unknowns (38.1%), while CMC hydrolysing strains (n=11) isolated from animals fed lichen and strains (n=4) isolated from animals fed silage where all characterised as B. fibrisolvens. None of the bacterial strains isolated from the rumen solids of reindeer fed lichen or silage were found to be cellulolytic. This study suggests that both lichen and timothy silage have a negative influence, compared to hay, on the numbers of cellulolytic bacteria adherent to the plant particles in the rumen of reindeer.

scholarly journals Toxic Indole Diterpenes from Endophyte-Infected Perennial Ryegrass Lolium perenne L.: Isolation and Stability

Toxins ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 16 ◽  
Author(s):  
Priyanka Reddy ◽  
Myrna A. Deseo ◽  
Vilnis Ezernieks ◽  
Kathryn Guthridge ◽  
German Spangenberg ◽  
...  
Keyword(s):  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Rapid Development ◽  
Toxicity Testing ◽  
Flash Chromatography ◽  
Preparative Hplc ◽  
Purification Method ◽  
Isolation And Purification ◽  
Lolium Perenne L ◽  
Lolitrem B

The most potent of the indole diterpenes, lolitrem B, is found in perennial ryegrass (Lolium perenne L.) infected with the endophyte Epichloë festucae var. lolii (also termed LpTG-1). Ingestion causes a neurological syndrome in grazing livestock called ryegrass staggers disease. To enable the rapid development of new forage varieties, the toxicity of lolitrem B and its biosynthetic intermediates needs to be established. However, most of these indole diterpenes are not commercially available; thus, isolation of these compounds is paramount. A concentrated endophyte-infected perennial ryegrass seed extract was subjected to silica flash chromatography followed by preparative HPLC and purification by crystallization resulting in lolitrem B and the intermediate compounds lolitrem E, paspaline and terpendole B. The four-step isolation and purification method resulted in a 25% yield of lolitrem B. After isolation, lolitrem B readily degraded to its biosynthetic intermediate, lolitriol. We also found that lolitrem B can readily degrade depending on the solvent and storage conditions. The facile method which takes into consideration the associated instability of lolitrem B, led to the purification of indole diterpenes in quantities sufficient for use as analytical standards for identification in pastures, and/or for toxicity testing in pasture development programs.

scholarly journals Studies on Indigenous Cotton linters for Preparation of Carboxymethyl Cellulose

1970 ◽  
Vol 42 (1) ◽  
pp. 29-36 ◽  
Author(s):  
Ismet Ara Jahan ◽  
Fahmida Sultana ◽  
M Nurul Islam ◽  
M Akram Hossain ◽  
Jainul Abedin
Keyword(s):  
Carboxymethyl Cellulose ◽  
Degree Of Substitution ◽  
Water Soluble ◽  
Suitable Method ◽  
Purification Method ◽  
Cellulosic Materials ◽  
Cotton Linters

Studies were carried out to find out a suitable method for preparation of carboxymethyl cellulose (CMC) from indigenous cotton linters. Special emphasis was given on purification method to remove most of the non-cellulosic materials. A twostep purification method of cotton linters for preparation of water soluble carboxymethyl cellulose (CMC) with degree of substitution (DS) 0.89 and viscosity 235 cp (0.5 % solution) have been described. Bangladesh J. Sci. Ind. Res. 42(1), 29-36, 2007

scholarly journals ISOLASI DAN SKRINING BAKTERI SELULOLITIK DARI BEBERAPA TANAH HUTAN DI BALI

2019 ◽  
Vol 7 (1) ◽  
pp. 11
Author(s):  
Ella Dewi Yusnia ◽  
Ida Bagus Wayan Gunam ◽  
Nyoman Semadi Antara
Keyword(s):  
Filter Paper ◽  
Congo Red ◽  
Carboxymethyl Cellulose ◽  
Soil Samples ◽  
Mangrove Forests ◽  
Decayed Wood ◽  
Bacterial Isolates ◽  
Cellulolytic Bacteria ◽  
Clear Zone ◽  
Solid Media

Soil is one habitats of the cellulolytic bacteria. Soil containing manure and decayed wood is habitation for cellulolytic bacteria.  This research is aimed to isolate and screen cellulolytic bacteria from the soil in Bali that has the potentials to degrade cellulose. The soil samples ware taken from forest in Gunaksa-Klungkung, Telaga-Karangasem, Sukahat-Karangasem, Gilimanuk-Jembrana, and Mangrove forests in Suwung-Denpasar. Each single cellulolytic bacteria colonies which grown on solid media containing carboxymethyl cellulose (CMC) were isolated. Screening of cellulolytic bacteria using congo red 0,1 %, staining bacterial isolates which were created clear zones in around the colony were selected. The isolation result are 67 isolated cellulolytic bacteria covered in five soils sample. Twenty-one bacterial isolates were producing cellulase enzymes with an indication of a clear zone in around the colony. Nine superior isolates have high cellulolytic index (CI), namely: G2-8 (5.41), G1-4 (4.86), G2-10 (4.5), G2-2 (3.64), M1-5 (3.10), G2-5 (3.03), M2-12 (2.72), G1-1 (2.38), and M1-1 (2.21). The obtained highest percentage of filter paper (Whatman No.1) degradation was 8.32% and the lowest was 2.48%. Keywords: forest soil, isolation, cellulolytic bacteria, congo red

Novel isolation and purification method permitting functional cytotoxicity studies of macrophages from milky spots in the greater omentum

1995 ◽  
Vol 184 (2) ◽  
pp. 253-261 ◽  
Author(s):  
Lambert F.G. Krist ◽  
Miranda Kerremans ◽  
Hans Koenen ◽  
Nico Blijleven ◽  
Inge L. Eestermans ◽  
...  
Keyword(s):  
Greater Omentum ◽  
Purification Method ◽  
Isolation And Purification ◽  
Cytotoxicity Studies ◽  
Milky Spots

scholarly journals Isolation and purification ofent-pimara-8(14),15-diene from engineeredAspergillus nidulansby accelerated solvent extraction combined with HPLC

2014 ◽  
Vol 6 (4) ◽  
pp. 1227-1234 ◽  
Author(s):  
K. Bromann ◽  
K. Viljanen ◽  
V. M. Moreira ◽  
J. Yli-Kauhaluoma ◽  
L. Ruohonen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Solvent Extraction ◽  
Aspergillus Nidulans ◽  
Accelerated Solvent Extraction ◽  
Purification Method ◽  
Isolation And Purification ◽  
Diterpene Hydrocarbon

This paper describes a purification method for tricyclic diterpene hydrocarbonent-pimara-8(14),15-diene produced inAspergillus nidulansand reports an antioxidant activity for this compound.

scholarly journals Preparative isolation and purification of seven compounds from Hibiscus mutabilis L. leaves by two-step high-speed counter-current chromatography

2015 ◽  
Vol 21 (2) ◽  
pp. 331-341 ◽  
Author(s):  
Zhuoni Hou ◽  
Xianrui Liang ◽  
Feng Su ◽  
Weike Su
Keyword(s):  
Ethyl Acetate ◽  
High Speed ◽  
Solvent System ◽  
Ionization Mass ◽  
Two Phase ◽  
Purification Method ◽  
Isolation And Purification ◽  
Chemical Structures ◽  
13C Nuclear Magnetic Resonance ◽  
Counter Current

Seven compounds from Hibiscus mutabilis L. leaves were first successfully achieved by two-step high-speed counter-current chromatography with two-phase solvent system composed of n-butanol-ethyl acetate-water (1:6:9, v/v/v) and n-hexane-ethyl acetate-methanol-water (3:5:3:5, v/v/v/v/). The critical experimental parameters of first-step separation were optimized with response surface methodology as follows: flow rate was 1.1 mL/min, revolution speed was 800 rpm and temperature was 30?C. Under the optimal conditions, around 5.0 mg of salicylic acid, 13.6 mg of rutin, 5.5 mg of genistein were obtained in 100 mg crude sample. Then, 9.2 mg of potengriffioside A, 4.7 mg of kaempferol 3-O-rutinoside, 3.0 mg of steppogenin and 2.5 mg of emodin were obtained by second-step separation. The purities of the seven compounds determined by UPLC were 96.2%, 93.8%, 95.4%, 94.3%, 98.0%, 94.1% and 90.8%, respectively. Their chemical structures were identified by electron spray ionization mass spectroscopy (ESI-MS) and 1H, 13C nuclear magnetic resonance (NMR). Furthermore, compound steppogenin and genistein were first reported from Hibiscus mutabilis L. The purification method was simple, efficient and evaded tedious separation process.

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