scholarly journals Effect of combination of cisplatin with brassinosteroids on the growth of cancer cells

2019 ◽  
Vol 63 (4) ◽  
pp. 437-444
Author(s):  
Olesya V. Panibrat ◽  
Vladimir N. Zhabinskii ◽  
Vladimir A. Khripach
Keyword(s):  
Cancer Cells ◽  
Human Lung ◽  
Antitumor Drugs ◽  
Tumor Cell Lines ◽  
Hep G2 ◽  
Negative Effects ◽  
Low Concentrations ◽  
Human Lung Carcinoma ◽  
Effective Doses ◽  
Natural Brassinosteroids

In this work, the effect of brassinosteroids on the antitumor activity of classical cytostatic cisplatin in tumor cell lines A549 (human lung carcinoma) and Hep G2 (human hepatocellular carcinoma) was evaluated. Natural brassinosteroids 24-epibrassinolide and 28-homocastasterone, as well as their synthetic analogues (22S,23S)-24-epibrassinolide and (22S,23S)-28-homocastasterone were used. All four compounds with cisplatin inhibited the growth of cancer cells more effectively than cisplatin alone. Combinations with low concentrations of synthetic brassinosteroids were more effecient, and at 1 µM decreased the IC50 of cisplatin by almost 2 times. The results suggest a possible benefit of combinations of classical antitumor drugs with brassinosteroids in overcoming the negative effects of chemotherapy by reducing their effective doses.

Therapeutically Potential of Medicago sativa Extracts. Chemical and in vitro assessments

2018 ◽  
Vol 69 (1) ◽  
pp. 121-124
Author(s):  
Iulia Pinzaru ◽  
Alina Heghes ◽  
Daniela Marti ◽  
Cristina Dehelean ◽  
Dorina Coricovac ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicago Sativa ◽  
Cytotoxic Effect ◽  
Human Lung ◽  
Polar Solvent ◽  
Total Phenols ◽  
Tumor Cell Lines ◽  
Human Breast ◽  
Human Lung Carcinoma

The present study was aimed to evaluate total phenols, flavonoid and flavonols content and to assess relative cytotoxicity of Medicago sativa hydro-alcoholic and alcoholic leaves and stems extracts on human lung carcinoma (A549) and human breast carcinoma (MDA-MB-231). All extracts tested have proven to be rich in hydroxylated compounds, larger amounts of phenolic compounds were found in extracts obtained using 70% ethanol, a proper polar solvent for this molecule types. Evaluation of antioxidant activity reveals values all most comparable with the ones of ascorbic acid. The extracts induced a cytotoxic effect on both tumor cell lines in a concentration-depend manner.

scholarly journals Cytotoxicity of single-walled carbon nanotubes to human lung carcinoma cells: The influence of N-acetylcysteine

2013 ◽  
Vol 21 (2) ◽  
pp. 59-61
Author(s):  
Nikola Jojic ◽  
Vesna Kojic ◽  
Danijela Kojic ◽  
Karmen Stankov ◽  
Gordana Bogdanovic
Keyword(s):  
Carbon Nanotubes ◽  
Lung Carcinoma ◽  
Human Lung ◽  
Single Walled Carbon Nanotubes ◽  
Carcinoma Cells ◽  
Lung Cells ◽  
Lung Carcinoma Cells ◽  
Low Concentrations ◽  
Human Lung Carcinoma ◽  
Walled Carbon Nanotubes

Background: Single-walled carbon nanotubes (SWCNTs) have been reported to induce cytotoxicity in different cell lines. Although the mechanisms underlying cytotoxicity are not fully understood, accumulation of reactive oxygen species (ROS) and oxidative damage is considered to be a likely contributing factor. Methods: Human lung carcinoma cells, A549, and human fetal lung fibroblasts, MRC-5 were used to assess the cytotoxicity of SWCNT in the presence and absence of a redox status regulator, N-acetylcysteine (NAC), via the MTT assay. Results: SWCNT induced a nearly three-fold greater loss of viability in A594 vs. MRC-5 cells at ?250 ?g/ml. SWCNT cytotoxicity at higher concentrations was similar for both cell lines, while NAC alone was non-toxic. The cytotoxicity of SWCNT (250 ?g/ml) in combination with NAC to A549 cells was significantly decreased at the lowest NAC concentration (1.5 ?g/ml), and was similar to NAC treatment alone at that concentration. Higher concentrations of NAC in combination with SWCNT (250 ?g/ml) resulted in increased cytotoxicity in both A549 and MRC-5 cells. Conclusion: A549 malignant lung cells are more susceptible to low concentrations of SWCNT vs. normal lung cells, and low concentrations of N-acetylcysteine appear to be cytoprotective, possibly due to its antioxidant properties.

scholarly journals Stemofurans X-Y from the Roots of Stemona Species from Laos

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901
Author(s):  
Dang Ngoc Quang ◽  
Vong Anatha Khamko ◽  
Nguyen Thi Trang ◽  
Lam Thi Hai Yen ◽  
Pham Huu Dien
Keyword(s):  
Hepatocellular Carcinoma ◽  
Breast Carcinoma ◽  
Cell Lines ◽  
Cancer Cell ◽  
Lung Carcinoma ◽  
Human Lung ◽  
Human Breast ◽  
Hep G2 ◽  
Human Lung Carcinoma ◽  
Epidermal Carcinoma

Two new phenylbenzofuran-type stilbenoids named stemofurans X and Y (1, 2) were isolated from the roots of Stemona pierrei and S. tuberosa, respectively, together with ten known compounds. These compounds were stemanthrenes B-C (3, 4), (+)-syringaresinol (5), maistemonine (6), isomaistemonine (7) and sesamin (8) from S. pierrei, and stemophenanthrenes A-C (9–11) and isopinosylvin A (12) from S. tuberosa. Stemofurans X-Y (1, 2) showed moderate cytotoxicity against the four cancer cell lines KB (human epidermal carcinoma), MCF7 (human breast carcinoma), SK-LU-1 (human lung carcinoma), and Hep-G2 (hepatocellular carcinoma).

scholarly journals Synthesis of New Biscoumarin Derivatives, In Vitro Cholinesterase Inhibition, Molecular Modelling and Antiproliferative Effect in A549 Human Lung Carcinoma Cells

2021 ◽  
Vol 22 (8) ◽  
pp. 3830
Author(s):  
Monika Hudáčová ◽  
Slávka Hamuľaková ◽  
Eva Konkoľová ◽  
Rastislav Jendželovský ◽  
Jana Vargová ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Line ◽  
Lung Carcinoma ◽  
Molecular Modelling ◽  
Antiproliferative Activity ◽  
Human Lung ◽  
A549 Cells ◽  
Cholinesterase Inhibition ◽  
Human Lung Carcinoma

A series of novel C4-C7-tethered biscoumarin derivatives (12a–e) linked through piperazine moiety was designed, synthesized, and evaluated biological/therapeutic potential. Biscoumarin 12d was found to be the most effective inhibitor of both acetylcholinesterase (AChE, IC50 = 6.30 µM) and butyrylcholinesterase (BChE, IC50 = 49 µM). Detailed molecular modelling studies compared the accommodation of ensaculin (well-established coumarin derivative tested in phase I of clinical trials) and 12d in the human recombinant AChE (hAChE) active site. The ability of novel compounds to cross the blood–brain barrier (BBB) was predicted with a positive outcome for compound 12e. The antiproliferative effects of newly synthesized biscoumarin derivatives were tested in vitro on human lung carcinoma cell line (A549) and normal colon fibroblast cell line (CCD-18Co). The effect of derivatives on cell proliferation was evaluated by MTT assay, quantification of cell numbers and viability, colony-forming assay, analysis of cell cycle distribution and mitotic activity. Intracellular localization of used derivatives in A549 cells was confirmed by confocal microscopy. Derivatives 12d and 12e showed significant antiproliferative activity in A549 cancer cells without a significant effect on normal CCD-18Co cells. The inhibition of hAChE/human recombinant BChE (hBChE), the antiproliferative activity on cancer cells, and the ability to cross the BBB suggest the high potential of biscoumarin derivatives. Beside the treatment of cancer, 12e might be applicable against disorders such as schizophrenia, and 12d could serve future development as therapeutic agents in the prevention and/or treatment of Alzheimer’s disease.

Inhibitory effects of Actinidia Chinensis planch root extracts (acRoots) on human lung cancer cells through retinoic acid receptor beta

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Lingyan Wang ◽  
Jiayun Hou ◽  
Minghuan Zheng ◽  
Lin Shi
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Human Lung ◽  
Retinoic Acid Receptor ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Inhibitory Effects ◽  
The Core ◽  
Human Lung Cancer Cells ◽  
Receptor Beta

Actinidia Chinensis Planch roots (acRoots) are used to treat many cancers, although the anti-tumor mechanism by which acRoots inhibit cancer cell growth remains unclear. The present study aims at investigating inhibitory effects of acRoots on human lung cancer cells and potential mechanisms. Our data demonstrate that the inhibitory effects of acRoots on lung cancer cells depend on genetic backgrounds and phenotypes of cells. We furthermore found the expression of metabolism-associated gene profiles varied between acRoots-hypersensitive (H460) or hyposensitive lung cancer cells (H1299) after screening lung cancer cells with different genetic backgrounds. We selected retinoic acid receptor beta (RARB) as the core target within metabolism-associated core gene networks and evaluated RARB changes and roles in cells treated with acRoots at different concentrations and timeframes. Hypersensitive cancer cells with the deletion of RARB expression did not response to the treatment with acRoots, while RARB deletion did not change effects of acRoots on hyposensitive cells. Thus, it seems that RARB as the core target within metabolism-associated networks plays important roles in the regulation of lung cancer cell sensitivity to acRoots.

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