scholarly journals Prevalensi Sistiserkosis pada Babi Hutan (Sus scrofa) yang dipotong di Tempat Pemotongan Hewan (TPH) Bengkulu Tengah, Bengkulu

2018 ◽  
Vol 6 (2) ◽  
pp. 24-31
Author(s):  
Noviriliensi Hartika ◽  
Elok Budi Retnani ◽  
Sri Murtini
Keyword(s):  
Immunosorbent Assay ◽  
Sus Scrofa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Post Mortem

Sistiserkosis/taeniasis merupakan penyakit zoonosis terabaikan yang memiliki dampak serius bagi ekonomi dan kesehatan masyarakat. Tujuan dari penelitian ini adalah untuk menentukan prevalensi sistiserkosis pada babi hutan di Bengkulu Tengah, Bengkulu. Penelitian ini dilakukan di tempat pemotongan hewan Bengkulu Tengah, Provinsi Bengkulu Indonesia antara Februari-Mei 2016. Delapan puluh dua ekor babi hutan di koleksi serum dari vena jugularis dan post-mortem karkasnya. Pemeriksaan post-mortem meliputi otot lidah, trisep, bisep, masseter, diafragma, intercostae, jantung, dan pemeriksaan hati untuk menentukan adanya sistiserkus. Serum di pisahkan dari darah masing-masing sampel dan di uji sirkulasi antigen dari sistiserkus menggunakan monoklonal antibodi-sandwich enzyme-linked immunosorbent assay (MOAB-ELISA). Hasil pemeriksaan post-mortem tidak di temukan adanya sistiserkus. Hasil uji ELISA didapatkan 8 sampel (9,8%) terdeteksi adanya antigen sistiserkus. Babi hutan dengan hasil seropositif sistiserkus ditemukan berasal dari kecamatan dengan prevalensi tertinggi kecamatan Pagar Jati (39,0%) diikuti oleh kecamatan Bang Haji (30,5%) dan prevalensi terendah ditemukan di kecamatan Pematang Tiga (30,5%). Penelitian ini menyatakan bahwa terdapat infeksi sistiserkus pada babi hutan dari Bengkulu Tengah, Bengkulu.

Immunodiagnostic potency of homologous antigens for natural Paramphistomum epiclitum infection in small ruminants in plate and paper enzyme linked immunosorbent assay

2017 ◽  
Author(s):  
Niranjan Kumar ◽  
Mehul M. Jadav ◽  
Bhupamani Das ◽  
Jaesh B. Solanki
Keyword(s):  
Molecular Weight ◽  
Immunosorbent Assay ◽  
Predictive Value ◽  
Small Ruminants ◽  
Enzyme Linked Immunosorbent Assay ◽  
Post Mortem ◽  
Secretory Antigen ◽  
Paramphistomum Epiclitum ◽  
Dot Elisa

The objective of the present work was to standardize and evaluate indirect plate and dot- enzyme linked immunosorbent assay (ELISA) using purified Paramphistomum epiclitum homologous antigens in the small ruminants. Electrophoretic separation of somatic antigen (PeSAg) in reducing condition on 15% polyacrylamide gel resolved into 16 proteins of the molecular weight ranging from 14 -100 kDa. Two step ethanolic precipitation of supernatant of in-vitro culture of the fluke yielded P. epiclitum excretory-secretory antigen (PeESAg) of molecular weight 28 kDa. The animals (Goats=123; Sheep=91) were broadly kept into post-mortem and faecal examined groups. At many occasion the PeSAg found to cross reacts with other helminths parasites thus minimizing the specificity of the tests and antigens. There was no any direct correlation between the parasites load and ELISA reactivity pattern. The noted prevalence rate after combining the results of post-mortem examination and PeESAg based ELISA (plate and paper/ dot) was 30.08% (37/123) in goats and 28.57% (26/91) in sheep. While using PeESAg, the calculated overall sensitivity% was 92.86 (goats)/ 100 (sheep) in both plate and dot-ELISA, specificity% was 91.58 (goats)/ 91.55 (sheep) in plate ELISA while 88.42 (goats)/ 92.96 (sheep) in dot-ELISA, positive predictive value% was 76.47 (goats)/ 76.92 (sheep) in plate ELISA while 70.27 (goats)/ 80 (sheep) in dot-ELISA and negative predictive value% was 97.75 (goats)/ 100 (sheep) in plate ELISA while 97.67 (goats)/ 100 (sheep) in dot-ELISA, these values were optimum for the field sera sample so the tests and PeESAg can be recommended for the detection P. epiclitum infection in the small ruminants.

scholarly journals Le diagnostic des encéphalopathies spongiformes chez les ruminants

2004 ◽  
Vol 17 (HS) ◽  
pp. 79-85
Author(s):  
F. LANTIER ◽  
D. MARC ◽  
P. SARRADIN ◽  
P. BERTHON ◽  
R. MERCEY ◽  
...  
Keyword(s):  
Western Blot ◽  
Immunosorbent Assay ◽  
Proteinase K ◽  
Enzyme Linked Immunosorbent Assay ◽  
Post Mortem ◽  
Système Nerveux Central ◽  
Système Nerveux

Le diagnostic des Encéphalopathies Spongiformes Transmissibles (EST) des ruminants est aujourd’hui basé sur la mise en évidence de l’isoforme pathologique, résistante à la protéinase K, de la protéine PrP, la PrPres. La PrPres peut être mise en évidence in situ, à l’aide de techniques immunohistochimiques ou, bien après extraction et traitement à la protéinase K, par des techniques immunochimiques (western blot, Enzyme Linked ImmunoSorbent Assay). Si le diagnostic de routine des encéphalopathies spongiformes animales a fait de gros progrès ces dernières années, il demeure un diagnostic post-mortem qui n’est applicable qu’aux tissus nerveux d’animaux suffisamment âgés pour permettre la formation de dépôts de PrPres dans le système nerveux central. La biopsie d’amygdales offre une possibilité de diagnostic ante-mortem chez les seuls animaux dont le tissu lymphoïde présente une accumulation de PrPres, les ovins génétiquement sensibles et la chèvre. Deux voies de développement des tests sont poursuivies actuellement : d’une part la mise au point d’un diagnostic sanguin précoce basé sur la détection de PrPres ou d’un autre marqueur spécifique de l’incubation d’une EST, et d’autre part des tests permettant de distinguer entre elles les souches de prion, notamment la souche Encéphalopathie Spongiforme Bovine (ESB). Ces évolutions constitueraient un progrès considérable pour notre compréhension des modes de transmission des EST et pour l’efficacité de la prophylaxie sanitaire.

scholarly journals Exames complementares no diagnóstico da tuberculose em bovinos reagentes à tuberculinização comparada

2016 ◽  
Vol 83 (0) ◽  
Author(s):  
Mariana Assunção de Souza ◽  
Nadia Grandi Bombonato ◽  
Pollyanna Mafra Soares ◽  
Gabriela Bim Ramos ◽  
Igor Paula Castro ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Post Mortem

RESUMO: Objetivou-se com este estudo comparar diferentes métodos complementares de diagnóstico (macroscópico, histopatológico, sorológico e molecular) da tuberculose, em bovinos naturalmente infectados. O trabalho deu-se por meio de amostras colhidas em abate sanitário de 40 bovinos reagentes no teste cervical comparativo (TCC) para tuberculose. A inspeção macroscópica post mortem das carcaças foi acompanhada de colheita de amostras de muco nasal, sangue e tecido (fígado, pulmão e linfonodo mediastínico) para realização do exame de reação em cadeia pela polimerase (PCR), de Enzyme-Linked Immunosorbent Assay (ELISA) e histopatológico com colorações de hematoxilina-eosina (HE) e Ziehl-Neelsen (ZN), respectivamente. Dos 40 bovinos inspecionados no abate, 22 (55%) animais apresentaram algum tipo de lesão macroscópica sugestiva de tuberculose. Nos achados histopatológicos visualizados em HE, 13 (32,5%) carcaças apontaram alterações histológicas, sendo 6 (15%) nos linfonodos mediastínicos, 5 (12,5%) no fígado e 3 (7,5%) no pulmão. Não foi observada a presença de bacilos álcool-ácido resistentes em nenhuma das amostras avaliadas. O ensaio sorológico de ELISA/IDEXX(r) identificou um (2,5%) animal reagente, e o teste de PCR detectou DNA de Mycobacterium bovis em uma (2,5%) amostra. Concluiu-se que entre os exames complementares de diagnóstico avaliados nenhum foi capaz de detectar todos os animais que estavam positivos na tuberculinização, porém a associação de diferentes métodos pode garantir a confiabilidade do diagnóstico.

scholarly journals Konsentrasi IL-6 Serum terhadap Penyembuhan Luka Pasca Pemasangan Implan Paduan Logam pada Babi (Sus scrofa)

2021 ◽  
Vol 9 (1) ◽  
pp. 21-29
Author(s):  
Nabila Latifa Hafizsha ◽  
Gunanti Gunanti ◽  
Deni Noviana ◽  
Sus Derthi Widhyari
Keyword(s):  
Analysis Of Variance ◽  
Immunosorbent Assay ◽  
Sus Scrofa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Data

Penelitian ini bertujuan untuk mengetahui gambaran konsentrasi IL-6 serum pada fase akut dan kronis selama proses penyembuhan luka pasca pemasangan implan paduan logam pada vesika urinaria babi. Penelitian ini menggunakan 6 ekor babi jantan dan betina, usia 2-3 bulan, dengan bobot badan berkisar 25-30 kg yang dibagi ke dalam dua kelompok implan dan tiga waktu pengamatan. Implan logam yang digunakan adalah Zn-0.5Al sebagai kelompok I dan ZnMg(4x) sebagai kelompok II, sedangkan waktu pengamatan dilakukan pada hari ke-0, 14, dan 28. Pemasangan implan dilakukan pada vesika urinaria (VU) menggunakan teknik cystotomi. Pengukuran konsentrasi IL-6 serum menggunakan metode enzyme-linked immunosorbent assay (ELISA). Data dianalasis menggunakan analysis of variance (ANOVA). Hasil penelitian menunjukkan pada hari ke-0, implan Zn-0.5Al dan ZnMg(4x) berturut-turut adalah 1.38±2.40 pg/mL dan 0.10±0.17 pg/mL. Konsentrasi IL-6 hari ke-14 pada setiap implan terlihat mengalami penurunan dibandingkan pada hari ke-0, yaitu 0.74±1.29 pg/mL dan 0 pg/mL. Selanjutnya hari ke-28 konsentrasi IL-6 kembali mengalami penurunan, yaitu 0.32±0.35 pg/mL dan 0 pg/mL. Penurunan konsentrasi IL-6 dari fase akut ke fase kronis proses penyembuhan luka pada setiap kelompok implan tidak berbeda signifikan (P>0,05). Pemeriksaan serum pada babi (Sus scrofa) terhadap konsentrasi IL-6 kelompok perlakuan implan Zn-0,5Al dan ZnMg(4x) tidak menunjukkan perbedaan nyata (P>0,05) pada hari ke-0, 14, dan 28.

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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