CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF TROPICAL BROWN ALGAE Padina australis FROM PRAMUKA ISLAND, DISTRICT OF SERIBU ISLAND, INDONESIA

2013 ◽  
Vol 5 (2) ◽  
Author(s):  
Joko Santoso ◽  
Fitriany Podungge ◽  
Heru Sumaryanto
Keyword(s):  
Antioxidant Activity ◽  
Dietary Fiber ◽  
Proximate Composition ◽  
Nutritional Value ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Ic50 Value ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

The proximate composition, dietary fiber, and total phenol contents as well as antioxidant activity of tropical brown alga Padina australis collected from the Pramuka Island, District of Seribu Island, Indonesia during the rainy season of 2011 were determined in order to evaluate their potential nutritional value and activity of natural antioxidant compound. The content of ash, protein, and fat were 22.26, 10.76, and 4.17 g/100 g dry matter, respectively; whereas the amounts of soluble, insoluble, and total dietary fibers were 8.4, 5.4, and 13.8 g/100 g, respectively. Methanol extract of P. australis contained the highest total phenol of 246.1 mg GAE/1000 g dry sample. The extract also had the highest activity on DPPH radical scavenging, measured by IC50 of 267.1 ppm. Both the total phenol and IC50 value extracts decreased in the following order: methanol > ethyl acetate > n-hexane.Keywords: antioxidant, dietary fiber, DPPH-scavenging, Padina australis, proximate composition

scholarly journals CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF TROPICAL BROWN ALGAE Padina australis FROM PRAMUKA ISLAND, DISTRICT OF SERIBU ISLAND, INDONESIA

2013 ◽  
Vol 5 (2) ◽  
Author(s):  
Joko Santoso ◽  
Fitriany Podungge ◽  
Heru Sumaryanto
Keyword(s):  
Antioxidant Activity ◽  
Dietary Fiber ◽  
Proximate Composition ◽  
Nutritional Value ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Total Phenol ◽  
Ic50 Value ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

<p>The proximate composition, dietary fiber, and total phenol contents as well as antioxidant activity of tropical brown alga Padina australis collected from the Pramuka Island, District of Seribu Island, Indonesia during the rainy season of 2011 were determined in order to evaluate their potential nutritional value and activity of natural antioxidant compound. The content of ash, protein, and fat were 22.26, 10.76, and 4.17 g/100 g dry matter, respectively; whereas the amounts of soluble, insoluble, and total dietary fibers were 8.4, 5.4, and 13.8 g/100 g, respectively. Methanol extract of P. australis contained the highest total phenol of 246.1 mg GAE/1000 g dry sample. The extract also had the highest activity on DPPH radical scavenging, measured by IC50 of 267.1 ppm. Both the total phenol and IC50 value extracts decreased in the following order: methanol &gt; ethyl acetate &gt; n-hexane.</p><p>Keywords: antioxidant, dietary fiber, DPPH-scavenging, Padina australis, proximate composition</p>

scholarly journals EFFECT OF INDOLE -3- ACETIC ACID ON CALLUS INDUCTION FROM LEAF EXPLANT OF OCIMUM BASILICUM LINN.

2015 ◽  
Vol 2 (2) ◽  
pp. 146-150
Author(s):  
Shillpa Sree V.N ◽  
Sirangeevi V ◽  
Deeksha S ◽  
Bhuvaneswari G ◽  
Ramya G ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Ocimum Basilicum ◽  
Dpph Radical ◽  
Ic50 Value ◽  
The Family ◽  
Half Strength ◽  
Dpph Radical Scavenging ◽  
Indole 3 Acetic Acid

Ocimum basilicum L. belongs to the family Lamiaceae; contains plenty of phytochemicals with significant nutritional as well as health benefits. Antioxidant activity of methanol extract of Ocimum basilicum callus was evaluated using various antioxidant assays. Proliferated callus was obtained on half-strength MS media supplemented with 1.40µM IAA. The callus extract showed considerable DPPH radical scavenging, hydroxyl radical scavenging and ferrous ion chelation activity with IC50 value of 16.05, 170.7 and 29.16µg/mL respectively.

scholarly journals ANTIOXIDANT ACTIVITY OF METHYL GALLATE ISOLATED FROM THE LEAVES OF Toona sureni

2010 ◽  
Vol 9 (3) ◽  
pp. 457-460 ◽  
Author(s):  
M. Taufik Ekaprasada ◽  
Hazli Nurdin ◽  
Sanusi Ibrahim ◽  
Dachriyanus Dachriyanus
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Methyl Gallate ◽  
Antioxidant Compound ◽  
Ic50 Value ◽  
Isolated Compound ◽  
Dpph Radical Scavenging

An antioxidant compound has been isolated from the leaves of Toona sureni (Blume) Merr. The structure was determined to be methyl 3,4,5-trihydroxybenzoate (methyl gallate), based on UV-vis, FTIR, NMR and MS spectra. The isolated compound exhibited potent antioxidant activity in the α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging test, with IC50 value 1.02 μg/mL.   Keywords: antioxidant activity, methyl gallate, Toona sureni

scholarly journals ANTIOXIDANT ACTIVITY OF RHIZOME EXTRACTS OF COLEUS FORSKOHLII BRIQ

2018 ◽  
Vol 11 (11) ◽  
pp. 275 ◽  
Author(s):  
Shanmugam S ◽  
Usha Rani V ◽  
Pradeep B V
Keyword(s):  
Antioxidant Activity ◽  
Tamil Nadu ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Coleus Forskohlii ◽  
Phytochemical Constituents ◽  
Polar Extracts ◽  
Dpph Radical Scavenging

Objective: The present study was on the analysis of the phytochemical constituents of Coleus forskohlii which was collected from five different areas in Tamil Nadu, where it is grown for commercial purposes.Methods: C. forskohlii tubers were collected from five areas (Thiruvannamalai, Kanchipuram, Kallakuruchi, Salem, and Vandalur) of Tamil Nadu. Dried tubers were extracted with different solvents such as aqueous, ethanol, and acetone (polar) and petroleum and chloroform (non-polar). Extracts were evaluated for total phenol and flavonoid content and antioxidant activity evaluation by 1,1-diphenyl-2-picrylhydrazyl (DPPH) reduction.Results: The highest DPPH radical scavenging activity (87.6%) was observed in ethanol extract of rhizome collected from Thiruvannamalai area. Total phenol and flavonoid contents were found to be, respectively, 38.82±0.22 mg gallic acid equivalents/g and 21.34±0.32 quercetin equivalent/g.Conclusion: The study clearly indicated the antioxidative potential of different extracts of C. forskohlii and this property may be useful in the preparation of drug formulations, health tonics, and cosmetics.

scholarly journals Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods

2020 ◽  
Vol 8 (12) ◽  
pp. 2749-2755
Author(s):  
Mohammad Afzal Hossain ◽  
Nadia Khan Disha ◽  
Jahid Hasan Shourove ◽  
Pappu Dey
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Tannin Content ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.

scholarly journals Antioxidant activity of extracted green algae silpau (Dyctyosphaeria versluysii)

2019 ◽  
Vol 11 (2) ◽  
Author(s):  
ENDANG S SRIMARIANA ◽  
DAN APITULEY
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Green Algae ◽  
Standard Method ◽  
Nutritional Value ◽  
Methanol Extract ◽  
Traditional Food ◽  
Phytochemical Content ◽  
Comprehensive Information ◽  
Ic50 Value

Abstract. Srimariana ES, Apituley DAN. 2019. Antioxidant activity of extracted green algae silpau (Dyctyosphaeria versluysii). Nusantara Bioscience 11: 153-156. Silpau (Dictyosphaeria versluysii) is a green algae that is widely available in Southwest Moluccas regency, living on coral reefs and not classified as seasonal plants. Silpau has long been used by local people, generally in the form of processed vegetables or colo-colo (traditional food). Except for its nutritional value, comprehensive information about silpau still unknown. Therefore, this study was carried out to determine its potential as an antioxidant. The phytochemical content of silpau was analyzed according to the standard method. The antioxidant activity of green algae silpau extract was carried out by reducing DPPH (1,1-diphenyl-2-picrylhydrazyl) free radicals. Phytochemical test of silpau revealed that silpau contain terpenoid compounds. The result of the study showed that the IC50 value of methanol extract of silpau was 547.97 ppm, indicated that silpau methanol extract categorized as a weak antioxidant.

scholarly journals Assessment of Total Phenolic, Flavonoid Content and Antioxidant Activity of Ocimum sanctum Linn

2019 ◽  
Vol 40 ◽  
pp. 30-35
Author(s):  
Ishwor Pathak ◽  
Muna Niraula
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Hexane Extract ◽  
Ocimum Sanctum ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Phenolic And Flavonoid

Ocimum sanctum, commonly known as Tulasi in Nepal, is a pharmacologically important plant due to its active constituents. In this work, extraction was carried out in hexane, chloroform and methanol solvents and their phytochemical screening was performed. Total phenolic and flavonoid contents in the plant were measured by Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method respectively. Antioxidant activity of the extracts was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Alkaloids, flavonoids, tannins, glycosides, polyphenols, terpenoids, tannins and steroids are mainly found in the extracts. Based on the result obtained, the plant posses a significant amount of total phenolic and total flavonoid content. Both phenolic and flavonoid contents were highest in methanol extract, followed by chloroform and hexane extract. Antioxidant activity of the extracts as ascorbic acid standard (IC50 value = 41.34 µg/mL) was in the order of methanol extract (IC50 value = 47.73 µg/mL) > chloroform extract (IC50 value = 79.46 µg/mL) > hexane extract (IC50 value = 94.68 µg/mL). The extent of the antioxidant activity of the plant is following the number of total phenolics and flavonoids present in it.

scholarly journals Total Phenolic and Coumarin Content, Antioxidant Activity of Leaves, Fruits, and Stem Barks of Grey Mangrove (Avicennia marina)

2020 ◽  
Vol 23 (2) ◽  
pp. 34-38
Author(s):  
Alik Kandhita Febriani ◽  
Ismiyarto Ismiyarto ◽  
Khairul Anam
Keyword(s):  
Antioxidant Activity ◽  
Skin Diseases ◽  
Radical Scavenging ◽  
Avicennia Marina ◽  
Total Phenolic ◽  
Mangrove Species ◽  
Traditional Medicines ◽  
Ic50 Value ◽  
Grey Mangrove ◽  
Dpph Radical Scavenging

Avicennia marina is one of the mangrove species used for traditional medicines. The leaves, fruits, and stem barks of A. marina are used for treating skin diseases. The stem barks are used for rheumatism, smallpox, and ulcers. The extract of A. marina was also reported to have antioxidant activity and indicates the presence of alkaloid, saponin, flavonoid, tannin, sterol/triterpenoid, and coumarin. However, the comparison of the antioxidant activity of leaves, fruits, and stem barks is not evaluated yet. The purpose of this study is to compare the antioxidant activity, total phenolic and coumarin content of leaves, fruits, and stem barks of A. marina. The antioxidant activity was determined using DPPH radical scavenging assay and was evaluated by spectrophotometric method at 515 nm. Quercetin was used for comparison. The fruits had the highest antioxidant activity with an IC50 value of 85.246 ppm, followed by stem barks and leaves with IC50 of 205.281 ppm and 307.037 ppm, respectively. Although the antioxidant activity of A. marina fruits was far from quercetin (IC50 of 3.789 ppm), it still categorized as a strong antioxidant. The strong antioxidant activity of fruits was followed by higher total phenolic and coumarin content than the stem barks and leaves part. Total phenolic and coumarin content of fruits were 49.119 mg GAE/ g and 8.894 x 10-3 mg CE/g, respectively. The leaves part had total coumarin content of 8.418 x 10-3 mg CE/g, but it had low IC50. It may be caused by the other secondary metabolite compounds that could reduce the antioxidant activity of coumarin.

scholarly journals Inhibition of Fe2+ Induced Lipid Peroxidation in Rats Brain by Extract of Euphorbia heterophylla in vitro

2019 ◽  
pp. 1-8
Author(s):  
Leye Jonathan Babatola ◽  
Oluwakemisola B. Oshanimi ◽  
Olanrewaju M. Oluba ◽  
Lawrence Okoror ◽  
Adewale Agboola Odutuga
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenol ◽  
Dependent Manner ◽  
Significant Difference

This study is sought to determine the antioxidant activity and protective ability of aqueous and methanol extractible phytochemicals from Euphorbia heterophylla leaves on lipid peroxidation induced in rat brain by pro-oxidant, in vitro. The extracts of the leaves were prepared, and the ability of the extracts is to inhibit 25 µM FeSO4 induced lipid peroxidation in isolated rats’ brain, were determined. Thereafter, total phenol content, reducing power (FRAP), Fe (II) chelating, and DPPH* free radical scavenging ability of the extracts was determined and considered as an index of antioxidant activity. The results revealed that the extracts inhibit malondialdehyde (MDA) production in the basal and pro-oxidant induced lipid peroxidised rats in a dose-dependent manner, [methanol 80.11%, aqueous 70.3%] with the methanol extract (MEE) significantly (P< 0.05) than that of aqueous extract (AEE). The methanol extract (0.74 ± 0.6 mg/g) had higher total phenol contents than the aqueous (0.57 ± 1.2 mg/g); likewise the methanol extract had higher reducing power (0.08 ± 0.2, 0.03 ± 0.1 mg/g), but had no significant difference in Fe (II) chelating ability (EC50= 0.34, 0.36) with DPPH* scavenging ability (EC50=0.075, 0.075). This antioxidant properties and the protective effect of this leaf could be harnessed in the management and prevention of degenerative diseases in association with oxidative stress.

scholarly journals Phytochemical Investigation and In Vitro Antioxidant Activities of Tetracarpidium conophorum (African Walnut) Seeds

2018 ◽  
Vol 6 (2) ◽  
pp. 56-61
Author(s):  
Kelly Oriakhi ◽  
Kissinger Orumwensodia ◽  
Patrick Uadia
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Crude Methanol Extract

Background: Tetracarpidium conophorum (African walnut) is an African plant with ethnobotanical uses. Objectives: The purpose of this study was to evaluate the phytochemical screening and in vitro antioxidant activities of methanol extract and fractions (F) [n-hexane (HEX-F), dichloromethane (DCM-F) and, ethyl acetate (EA-F)] of T. conophorum seeds. Methods: Phytochemical screening and in vitro antioxidant activity study were carried out using DPPH, ABTs radical scavenging assays, nitric oxide inhibitory and reducing potential assays. Results: Methanol extract and its fractions contain phenols, flavonoids, saponins, tannins, terpenoids, and alkaloids. The concentrations of total phenols and flavonoids content were significantly higher in EA-F and crude methanol extract compared to other fractions. Crude methanol and EA-F contain higher concentrations of tannin while hexane fraction had the lowest tannin content but relatively higher proanthocyanidin content compared to other fractions. The antioxidant activity study showed that both methanol crude extract and fractions of T. conophorum seeds have significant activities for DPPH radical scavenging, reducing power, ferric reducing antioxidant potential, nitric oxide inhibitory activities, ABTS and hydroxyl radical scavenging for. DPPH radical scavenging activities of EA-F showed the lowest IC50 of 33.11 µg/mL, followed by Hex-F, DCM-F and crude methanol extract with IC50 of 33.43, 42.09 and 45.44 µg/mL, respectively, when compared to ascorbic acid with IC50 of 17.08 µg/mL. Conclusion: The study showed that T. conophorum seed is a rich source of secondary metabolites, which may be responsible for its antioxidant activities.

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