scholarly journals Ability of Bacteria Isolated from Black Water Ecosystem of Central Kalimantan in Degrading of Crude Oil and Diesel Oil

2001 ◽  
Vol 2 (2) ◽  
pp. 1-7
Author(s):  
Iswandi Anas ◽  
Noegroho Hadi ◽  
Dwi Andreas Santosa
Keyword(s):  
Crude Oil ◽  
Liquid Medium ◽  
Diesel Oil ◽  
Soil Biology ◽  
Central Kalimantan ◽  
Biology Department ◽  
Black Water ◽  
Water Ecosystem ◽  
Degrading Bacteria ◽  
Minimum Medium

The objective of this experiment was to evaluate the abili!y of bacteria strains isolated from black water ecosystem of Central Kalimantan in degrading crude oil and diesel oil. The experiment was conducted at the Laboratory of Soil Biology, Department of Soil Sciences, Faculty ~f Agriculture, BogorAgricultural University (IPB). The crude oil degrading ability of bacteria was tested on a minimum liquid medium (Gurujeyalakshmi dan Orial, 1989) and soil. Six crude oil and diesel oil degrading bacteria were isolated. The bacteria were identified as Bacillus panthotenticus (2 strains), B. circulars (1 strain), Pssudomonas diminuta (1 strain), P. stufzeri (1strain) and Klebsiella edwardsii (1 strain). These bacteria were able to degrade crude oil or diesel oil whether in a liquid minimum medium or in soil.On a minimum liquid medium, after 10 days incubation, bacteria were able to degrade 20.49% of the crude oil, while in the Entisol soil, after 7 days of incubation, as high as 40.29% of crude oil was degraded and after 28 days incubation, 64.95 of crude oil had been degraded by selected bacteria.

scholarly journals Ability of Bacteria Isolated from Black Water Ecosystem of Central Kalimantan in Degrading of Crude Oil and Diesel Oil

2001 ◽  
Vol 2 (2) ◽  
Author(s):  
Iswandi Anas ◽  
Noegroho Hadi ◽  
Dwi Andreas Santosa
Keyword(s):  
Crude Oil ◽  
Liquid Medium ◽  
Diesel Oil ◽  
Soil Biology ◽  
Central Kalimantan ◽  
Biology Department ◽  
Black Water ◽  
Water Ecosystem ◽  
Degrading Bacteria ◽  
Minimum Medium

The objective of this experiment was to evaluate the abili!y of bacteria strains isolated from black water ecosystem of Central Kalimantan in degrading crude oil and diesel oil. The experiment was conducted at the Laboratory of Soil Biology, Department of Soil Sciences, Faculty ~f Agriculture, BogorAgricultural University (IPB). The crude oil degrading ability of bacteria was tested on a minimum liquid medium (Gurujeyalakshmi dan Orial, 1989) and soil. Six crude oil and diesel oil degrading bacteria were isolated. The bacteria were identified as Bacillus panthotenticus (2 strains), B. circulars (1 strain), Pssudomonas diminuta (1 strain), P. stufzeri (1strain) and Klebsiella edwardsii (1 strain). These bacteria were able to degrade crude oil or diesel oil whether in a liquid minimum medium or in soil.On a minimum liquid medium, after 10 days incubation, bacteria were able to degrade 20.49% of the crude oil, while in the Entisol soil, after 7 days of incubation, as high as 40.29% of crude oil was degraded and after 28 days incubation, 64.95 of crude oil had been degraded by selected bacteria.

scholarly journals Isolation and Activity Test of Acidophilic Iron and Sulfur Oxidizing Bacteria from Black Water Ecosystem of Central Kalimantan

2004 ◽  
Vol 6 (2) ◽  
pp. 51-56
Author(s):  
Nurseha . ◽  
Gunawan Djajakirana
Keyword(s):  
Leaching Tests ◽  
Sulfide Ores ◽  
Central Kalimantan ◽  
Black Water ◽  
Ferrous Salt ◽  
Activity Test ◽  
Water Ecosystem ◽  
Sulfur Oxidizing ◽  
Minimal Media ◽  
Sulfur Oxidizing Bacteria

The acidophilic iron and sulfur oxidizing bacteria were isolatedfrom black water ecosystem, an 'extreme' ecosystemaffected indirect or directly by peat land Isolation and selection were done on minimal media (liquid and solid). All selectedstrain of bacteria (BB 179, OM 349, AH 41, TB 23, TB 27, TP 3, NN 323, and SI 188) were identified as Thiohacillusferrooxidans. Biooxidation and bio-leaching tests were accomplished using the isolated bacteria. The results showed thecapability of the isolated bacteria to oxidize ferrous-salt and to leach the low qualities of sulfide ores.

Novel diesel-oil-degrading bacteria and fungi from the Ecuadorian Amazon rainforest

2015 ◽  
Vol 71 (10) ◽  
pp. 1554-1561 ◽  
Author(s):  
N. R. Maddela ◽  
M. Masabanda ◽  
M. Leiva-Mora
Keyword(s):  
Crude Oil ◽  
Diesel Oil ◽  
Amazon Rainforest ◽  
Total N ◽  
Soil Enrichment ◽  
North East ◽  
Enrichment Technique ◽  
Oil Exploitation ◽  
Degrading Bacteria ◽  
Bacteria And Fungi

Isolating new diesel-oil-degrading microorganisms from crude-oil contaminated sites and evaluating their degradation capacities are vitally important in the remediation of oil-polluted environments and crude-oil exploitation. In this research, new hydrocarbon-degrading bacteria and fungi were isolated from the crude-oil contaminated soil of the oil-fields in the Amazon rainforest of north-east Ecuador by using a soil enrichment technique. Degradation analysis was tracked by gas chromatography and a flame ionization detector. Under laboratory conditions, maximum degradability of the total n-alkanes reached up to 77.34 and 62.62 removal ratios after 30 days of incubation for the evaporated diesel oil by fungi (isolate-1) and bacteria (isolate-1), respectively. The 16S/18S rDNA sequence analysis indicated that the microorganisms were most closely (99–100%) related to Bacillus cereus (isolate-1), Bacillus thuringiensis (isolate-2), Geomyces pannorum (isolate-1), and Geomyces sp. (isolate-2). Therefore, these strains enable the degradation of hydrocarbons as the sole carbon source, and these findings will benefit these strains in the remediation of oil-polluted environments and oil exploitation.

scholarly journals Effects of Dispersants and Biosurfactants on Crude-Oil Biodegradation and Bacterial Community Succession

2021 ◽  
Vol 9 (6) ◽  
pp. 1200
Author(s):  
Gareth E. Thomas ◽  
Jan L. Brant ◽  
Pablo Campo ◽  
Dave R. Clark ◽  
Frederic Coulon ◽  
...  
Keyword(s):  
Crude Oil ◽  
Early Stage ◽  
Niche Partitioning ◽  
Hydrocarbon Biodegradation ◽  
Hydrocarbonoclastic Bacteria ◽  
Bacterial Response ◽  
Oil Biodegradation ◽  
Interfacial Surface ◽  
Degrading Bacteria ◽  
Oil Spill Response

This study evaluated the effects of three commercial dispersants (Finasol OSR 52, Slickgone NS, Superdispersant 25) and three biosurfactants (rhamnolipid, trehalolipid, sophorolipid) in crude-oil seawater microcosms. We analysed the crucial early bacterial response (1 and 3 days). In contrast, most analyses miss this key period and instead focus on later time points after oil and dispersant addition. By focusing on the early stage, we show that dispersants and biosurfactants, which reduce the interfacial surface tension of oil and water, significantly increase the abundance of hydrocarbon-degrading bacteria, and the rate of hydrocarbon biodegradation, within 24 h. A succession of obligate hydrocarbonoclastic bacteria (OHCB), driven by metabolite niche partitioning, is demonstrated. Importantly, this succession has revealed how the OHCB Oleispira, hitherto considered to be a psychrophile, can dominate in the early stages of oil-spill response (1 and 3 days), outcompeting all other OHCB, at the relatively high temperature of 16 °C. Additionally, we demonstrate how some dispersants or biosurfactants can select for specific bacterial genera, especially the biosurfactant rhamnolipid, which appears to provide an advantageous compatibility with Pseudomonas, a genus in which some species synthesize rhamnolipid in the presence of hydrocarbons.

scholarly journals CRUDE OIL BIOREMEDIATION BY INDIGENOUS BACTERIA ISOLATED FROM OILY SLUDGE

2016 ◽  
Vol 78 (11-2) ◽  
Author(s):  
Nur Hafizah Azizan ◽  
Kasing Ak Apun ◽  
Lesley Maurice Bilung ◽  
Micky Vincent ◽  
Hairul Azman Roslan ◽  
...  
Keyword(s):  
Crude Oil ◽  
Enrichment Culture ◽  
Molecular Techniques ◽  
Oily Sludge ◽  
Minimal Salt Medium ◽  
Alkane Degradation ◽  
Growth Study ◽  
The Third ◽  
Cell Counts ◽  
Degrading Bacteria

Enrichment culture technique leads to the discovery of six presumptive TPH-degrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques have successfully isolated Pseudomonas aeruginosa (UMAS1PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF). All strains were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt medium supplemented with 1% (v/v) crude oil. Growth study showed that they produced the highest cell counts on the third or fourth day by 108 – 1011 CFU/ml. Six artificial consortium inoculums have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 – C24. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader with degradation percentage between 55 – 90% of n-C14 – C18 in 14 days. This was followed by UMAS1PF and UMAS3KF with 11 – 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2:B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, A1:B2, B+NPK and A2:B3. Result showed that UMAS1PF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2:B3 showed the highest PAHs degradation, followed by plots A1:B2, A3:B1:C2 and A1:C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.

scholarly journals Preparation of Sulfur-Free Exfoliated Graphite by a Two-Step Intercalation Process and Its Application for Adsorption of Oils

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Jun He ◽  
Laizhou Song ◽  
Hongxia Yang ◽  
Xiaohui Ren ◽  
Lifei Xing
Keyword(s):  
Crude Oil ◽  
Perchloric Acid ◽  
Weight Ratio ◽  
Intercalation Compound ◽  
Diesel Oil ◽  
Chemical Oxidation ◽  
Exfoliated Graphite ◽  
Bet Surface Area ◽  
Graphite Flake ◽  
Intercalation Process

The sulfur-free exfoliated graphite (EG) was prepared by a two-step chemical oxidation process, using natural flake graphite (NFG) as the precursor. The first chemical intercalation process was carried out at a temperature of 30°C for 50 min, with the optimum addition of NFG, potassium permanganate, and perchloric acid in a weight ratio of 1 : 0.4 : 10.56. Then, in the secondary intercalation step, dipotassium phosphate was employed as the intercalating agent to further increase the exfoliated volume (EV) of EG. NFG, graphite intercalation compound (GIC), and EG were characterized by scanning electron microscope (SEM), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), Fourier transform infrared spectrometer (FTIR), BET surface area, and porosity analyzer. Also, the uptakes of crude oil, diesel oil, and gasoline by EG were determined. Results show that perchloric acid and hydrogen phosphate are validated to enter into the interlayer of graphite flake. The obtained EG possesses a large exfoliated volume (EV) and has an excellent affinity to oils; thus, the material has rapid adsorption rates and high adsorption capacities for crude oil, diesel oil, and gasoline.

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