Diagnostic Value of Serum Biomarkers in Combined Hepatocelluar-Cholangiocarcinoma

AbstractThe aim of this study was to determine whether neutrophil CD64 (nCD64) combined with procalcitonin (PCT), C-reactive protein (CRP) and white blood cell count (WBC) can increase the sensitivity and accuracy of neonatal sepsis diagnosis.The serum levels of nCD64, CRP, PCT and WBC were detected in 60 patients with neonatal sepsis and 60 patients with non-sepsis. Sensitivity, specificity, positive and negative predictive values, receiver operating characteristic (ROC) area under the curve (AUC), and logistic regression analysis were performed to evaluate the diagnostic value of these markers on neonatal sepsis.Serum levels of nCD64, PCT, CRP and WBC were higher in the sepsis group than non-sepsis group (p<0.001). The sensitivities of nCD64, PCT, CRP and WBC at the recommended cut-off level for all infants were 79.5%, 68.2%, 38.6% and 52.3%, respectively. The best combination was nCD64 and PCT, which obtained sensitivity of 90.9%, largest AUC of 0.922, and a negative predictive value of 89.2%. However by using an optimal cut-off value, the sensitivities of all four biomarkers for the diagnosis of neonatal sepsis were increased to 95.5%. Except for WBC, the birth weight and gestational age had no effects on the diagnostic value of these serum biomarkers.nCD64 and PCT are better diagnostic biomarkers for early diagnosis of neonatal sepsis as compared to CRP. With the help of optimal cut-off value based on ROC curve and logistic regression analysis, the combination of these biomarkers could improve the sensitivity for the diagnosis of suspected late-onset neonatal sepsis based on common serum biomarkers.

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The diagnostic accuracy of biomarkers for diagnosis of primary biliary cholangitis (PBC) in anti-mitochondrial antibody (AMA)-negative PBC patients: a review of literature

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2017-0249 ◽

2017 ◽

Vol 56 (1) ◽

Cited By ~ 7

Author(s):

Federica de Liso ◽

Caterina Matinato ◽

Mariangela Ronchi ◽

Rita Maiavacca

Keyword(s):

Diagnostic Value ◽

Serum Biomarkers ◽

Primary Biliary Cholangitis ◽

Nuclear Pore ◽

Biliary Cirrhosis ◽

Mean Values ◽

Nuclear Antigens ◽

Diagnostic Role ◽

New Biomarkers ◽

Mitochondrial Antibody

AbstractPrimary biliary cholangitis (PBC), also known as primary biliary cirrhosis, is an autoimmune disease of the liver characterized by anti-mitochondrial antibodies (AMA) in 90%–95% of patients. The aim of this study was to evaluate the diagnostic value of several serum biomarkers in patients with PBC but negative for AMA. Some antinuclear antibodies (ANA) pattern, detected by indirect immunofluorescence (IIF), such as multiple nuclear dot (MND) and rim-like patterns are well-known to be specific for PBC. The corresponding nuclear antigens are the components of the nuclear pore complex (Gp210 for rim-like pattern) and Sp100, PML proteins (for MND pattern) detectable by immunoblotting and ELISA methods. More recently, new biomarkers have been evaluated in order to improve the diagnostic sensitivity, such as kelch-like 12 (KLHL12) and hexokinase-1. Considering these different serum biomarkers, studies evaluating their diagnostic role in AMA-negative PBC patients compared to AMA-positive ones and controls were included in this review. Pooled sensitivity and specificity were 37% and 85%, respectively. The overall PPV and NPV mean values were 45% and 83%. Even if all biomarkers are very specific for PBC, the overall sensitivity was poor and therefore is necessary to identify a marker with a greater sensitivity for PBC in AMA-negative patients.

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Diagnostic Value of Serum Biomarkers for Patients Undergoing Curative Resection with Non-B, Non-C Hepatocellular Carcinoma

Journal of College of Physicians And Surgeons Pakistan ◽

10.29271/jcpsp.2020.02.134 ◽

2020 ◽

Vol 30 (02) ◽

pp. 134-138

Author(s):

Youdi Li ◽

Yanfei Chen ◽

Jiu Chen

Keyword(s):

Hepatocellular Carcinoma ◽

Curative Resection ◽

Diagnostic Value ◽

Serum Biomarkers

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Diagnostic Value of Multiple Serum Biomarkers for Vancomycin-Induced Kidney Injury

Journal of Clinical Medicine ◽

10.3390/jcm10215005 ◽

2021 ◽

Vol 10 (21) ◽

pp. 5005

Author(s):

Sang-Mi Kim ◽

Hyun-Seung Lee ◽

Min-Ji Kim ◽

Hyung-Doo Park ◽

Soo-Youn Lee

Keyword(s):

Cystatin C ◽

Tumor Necrosis Factor Receptor ◽

Area Under The Curve ◽

Kidney Injury ◽

Diagnostic Value ◽

Serum Biomarkers ◽

Retinol Binding Protein ◽

Control Group ◽

Strong Negative Correlation ◽

Retinol Binding Protein 4

Acute kidney injury (AKI) is a major contributor to in-hospital morbidity and mortality. Vancomycin, one of the most commonly used antibiotics in a clinical setting, is associated with AKI, with its incidence ranging up to 43%. Despite the high demand, few studies have investigated serum biomarkers to detect vancomycin-induced kidney injury (VIKI). Here, we evaluated the diagnostic value of nine candidate serum biomarkers for VIKI. A total of 23,182 cases referred for vancomycin concentration measurement from January 2018 to December 2019 were screened and 28 subjects with confirmed VIKI were enrolled (VIKI group). Age- and sex- matched control group consisted of 21 subjects who underwent vancomycin therapy without developing VIKI (non-VIKI group), and 23 healthy controls (HC group). The serum concentrations of clusterin, retinol binding protein 4 (RBP4), interleukin-18 (IL-18), tumor necrosis factor receptor 1 (TNF-R1), C-X-C motif chemokine ligand 10 (CXCL10), neutrophil gelatinase-associated lipocalin (NGAL), osteopontin, trefoil factor-3 (TFF3), and cystatin C were compared among the three groups, and their correlations with estimated glomerular filtration rate (eGFR) and diagnostic values for VIKI were assessed. All of the biomarkers except clusterin and RBP4 exhibited significant elevation in the VIKI group. Serum TFF3, cystatin C, TNF-R1, and osteopontin demonstrated an excellent diagnostic value for VIKI (TFF3, area under the curve (AUC) 0.932; cystatin C, AUC 0.917; TNF-R1, AUC 0.866; osteopontin, AUC 0.787); and except osteopontin, a strong negative correlation with eGFR (TFF3, r = −0.71; cystatin C, r = −0.70; TNF-R1, r = −0.60). IL-18, CXCL10, and NGAL showed weak correlation with eGFR and moderate diagnostic value for VIKI. This study tested multiple serum biomarkers for VIKI and showed that serum TFF3, cystatin C, TNF-R1, and osteopontin could efficiently discriminate VIKI patients. Further studies are warranted to clarify the diagnostic value of these biomarkers in VIKI.

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Serum Biomarkers for Noninvasive Diagnosis of Liver Diseases: How laudable are these tools?

Current Chemical Biology ◽

10.2174/2212796814999201111204639 ◽

2020 ◽

Vol 14 ◽

Author(s):

Ankita Singh ◽

Vipul Ranjan ◽

Rina Das ◽

Karun Bhatti ◽

Dinesh Kumar Mehta ◽

...

Keyword(s):

Liver Biopsy ◽

Liver Diseases ◽

Liver Parenchyma ◽

Diagnostic Value ◽

Minimal Invasive ◽

Serum Biomarkers ◽

Diagnostic Tools ◽

Geographic Population ◽

Monitoring Therapy ◽

Noninvasive Biomarkers

Abstract:: Innumerable reasons have been reported to affect and infect the liver and cause liver diseases. The evaluation and follow up of liver fibrosis and cirrhosis have been traditionally performed by liver biopsy. However, it has become evident that this once defined as “gold-standard” is now not the best method as it involves many limitations. Attempts to reveal non-invasive diagnostic tools have generated serum biomarkers, multiple scores, formulae, and imaging modalities. All are better tolerated, safer, more acceptable to the patient, and are less expensive than liver biopsy. Biomarkers have various advantages like minimal invasive, easy to apply with great availability and easier reproducibility, useful for monitoring therapy and less expensive. But then, direct biomarkers involved in extra cellular matrix turnover, need further validation in different geographic population and indirect biomarkers may not predict early pathophysiological changes in liver parenchyma. The accuracy and diagnostic value of most, if not all, of these biomarkers remains controversial. Hence, there is a need for biomarker which is specific for liver and can identify magnitude of clinical outcome of the disease. In this review, we discuss the clinical utility, limitations, and development of noninvasive biomarkers in their use as diagnostic and prognostic tests and analyze whether the present known serum biomarkers are laudable and accurate tools for diagnosis of liver diseases.

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Comparison of serum biomarkers for the early diagnosis of patients with liver cirrhosis and systemic inflammatory response syndrome

10.21203/rs.2.10101/v1 ◽

2019 ◽

Author(s):

Danhong Yang ◽

Yuanjun Xie ◽

Qiang Zhang ◽

Yicheng Huang ◽

Yining Dai ◽

...

Keyword(s):

Liver Cirrhosis ◽

Systemic Inflammatory Response Syndrome ◽

Early Diagnosis ◽

Inflammatory Response ◽

Diagnostic Value ◽

Serum Biomarkers ◽

Systemic Inflammatory Response ◽

Neutrophil Percentage ◽

Tnf Α ◽

Wbc Count

Abstract Background Systemic inflammatory response syndrome (SIRS) can cause serious negative effects among patients with liver cirrhosis (LC). . It is very important to finding methods for early diagnose and intervene early in these patients.This study was to assess the accuracy of early diagnostic value of serum biomarkers in patients with LC and SIRS. Methods A total of 123 LC patients were enrolled, 64 of whom were diagnosed with SIRS and 59 patients without SIRS. Various biomarkers and cytokines were measured in two groups of patients: LC+SIRS and LC−SIRS. Receiver operating characteristic curves (ROCs) were used to assess the ability of tested biomarkers to diagnoseLC with SIRS. Results White blood cell (WBC) count, neutrophil percentage(N%), as well as levels of C-reactive protein (CRP), procalcitonin(PCT), soluble triggering receptor expressed on myeloid cells-1 (sTREM-1), interleukin (IL)-6, IL-10 and tumor necrosis factor (TNF)-α, were significantly higher in the LC+SIRS group than in the LC−SIRS group. But only sTREM-1 had high accuracy of the early diagnosis for LC+SIRS. The WBC count, N% as well as levels of CRP, PCT, IL-6, andTNF-α had moderate diagnostic value, and IL-10 had low diagnostic value.The cutoff value of sTREM-1was 179.23 pg/mL and showed 84.4% sensitivity and 93.2% specificity.The AUC of sTREM-1 was 0.904(95%CI 0.899–0.982) and higher than that of the WBC count as well as levels of CRP, PCT, IL-6, IL-10 and TNF-α. In addition, 24 (37.50%) LC+SIRS cases died during 90-day follow-up. Neutrophil percentage as well as levels of CRP, PCT, sTREM-1, IL-6 and TNF-α were significantly higher those who died than in those who survived. Conclusion The WBC count,N% and levels of CRP, PCT, sTREM-1, IL-6, IL-10 and TNF-α are helpful for the early diagnosis of LC+SIRS.Serum sTREM-1 cut-off levels provide better accuracy than customary levels for cirrhosis with SIRS and appears to be a useful early marker to discriminate between SIRS and no-SIRS.It may also be helpful for implications in the prevention and treatment of cirrhosis and SIRS/sepsis.

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The informative value of assessment of direct serum biomarkers for diagnosis of various stages of liver fibrosis in children

Российский педиатрический журнал ◽

10.46563/1560-9561-2021-24-1-4-11 ◽

2021 ◽

Vol 24 (1) ◽

pp. 4-11

Author(s):

Elena A. Kulebina ◽

Andrey N. Surkov ◽

Andrey P. Fisenko ◽

Alexander S. Potapov ◽

Natalya M. Alyabeva ◽

...

Keyword(s):

Liver Fibrosis ◽

Diagnostic Value ◽

Serum Biomarkers ◽

Curve Analysis ◽

Serum Concentrations ◽

Chronic Liver Diseases ◽

Roc Curve Analysis ◽

Diagnostic Significance ◽

Single Center Study ◽

Specific Outcome

Introduction. Serum concentrations of collagens I, III, IV types (K-I, K-III, K-IV) and hyaluronic acid (HA) are reported to be informative in terms of noninvasive diagnosis liver fibrosis stages. In pediatrics, there is not enough data on this. Purpose was to assess the diagnostic value of direct biomarkers in predicting the stage of fibrosis in children with chronic liver diseases (CLD). Materials and methods. A prospective single-center study included 80 children with CLD . All patients underwent marginal resection of liver tissue under laparoscopic control. The serum K-I, K-IV, and HA concentration were measured by enzyme immunoassay. The authors applied ROC curve analysis to assess quantitative signs’ diagnostic significance in predicting a specific outcome. Results.The optimal KI value for the diagnosis of cirrhosis was 144.24 ng/ml, with AUROC: 0.758 ± 0.101 with 95% CI: 0.560-0.957, sensitivity and specificity 65.2% and 77.8%, respectively. The optimal K-IV values for the diagnosis of moderate fibrosis and cirrhosis were 11.29 ng/ml and 27.40 ng/ml, respectively, with AUROC 0.807 ± 0.092 with 95% CI: 0.627-0.987, 0.685 ± 0.062 with 95% CI: 0.567-0.810, sensitivity 82.4% and 61.15%, specificity 66.7%, and 64.7%, respectively. The optimal BG values for the diagnosis of weak and moderate fibrosis were 34.9 ng/ml and 36.5 ng/ml, for cirrhosis 38.3 ng/ml, with AUROC 0.912 ± 0.050 with 95% CI: 0.815-1.00; 0.849 ± 0.064 with 95% CI: 0.723-0.974, and 0.825 ± 0.048 with 95% CI: 0.730-0.920, respectively. Sensitivity was 84.6% at all stages, specificity - 77.8%, 61.5% and 70.6%, respectively. Conclusions. LF biomarkers have diagnostic significance in the detection of the stages of liver fibrosis. LF biomarkers are informative, reproducible noninvasive indices in the diagnosis of liver fibrosis in children.

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