scholarly journals Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE UV-Vis analysis, variable signal analysis, sensor sensitivity and SEM-EDS

2021 ◽  
Vol 7 (SpecialIssue) ◽  
pp. 370-376
Author(s):  
Abd Hakim S
Keyword(s):  
Enzyme Immobilization ◽  
Phosphate Buffer ◽  
Signal Analysis ◽  
Indicator Electrode ◽  
Detection Range ◽  
Eds Analysis ◽  
Urease Enzyme ◽  
Uv Visible ◽  
Absorbance Spectra

This study aims to characterize the phosphate buffer and urease enzymes through UV-Vis and SEM-EDS absorbance spectra using tungsten as an indicator electrode. The method used is a potentiometric biosensor with urease enzyme immobilization technique for urea analyte. A small detection range of 10-5-10-4M has been studied with PVA-enzyme coated indicator electrodes coated with PVC-KTpClPB. On this basis, the researchers increased the detection range by analyzing glutaraldehyde (GA) mixed with PVA-enzyme and o-NPOE mixed with PVC-KTpClPB. The best results of GA mixed PVA-enzyme at GA2.9% UV-Visible analysis. The best results were PVA-enzyme coated indicator electrodes coated with GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS analysis on PVA-enzyme samples 3x coated with GA 1x and PVC-KTpClPB-o-NPOE 1x with o-NPOE variation of 61% and 66%.

scholarly journals The Use of Tungsten in Potentiometry to Detect Pospat Baffer and Urease Enzyme

2021 ◽  
Vol 7 (3) ◽  
pp. 325
Author(s):  
Victor H Sihombing ◽  
Abd Hakim S
Keyword(s):  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Electrolyte Solutions ◽  
Indicator Electrode ◽  
State University ◽  
Buffer Solution ◽  
Urea Content ◽  
Ftir Characterization ◽  
Urease Enzyme

This study aims to characterize phosphate buffer and urease enzymes through the absorbance spectrum of UV-Vis and FTIR using tungsten as the indicator electrode. The method used in this research is the biosensor potentiometric method carried out in the Laboratory of the Faculty of Mathematics and Natural Sciences, State University of Medan and the Beacukai Belawan Medan laboratory. The absorbance characterization of electrolyte solutions in various compositions using UV-Vis showed that phosphate buffer solution 0.001 M pH 7.5+KCl 0.001 M + urea 0.001 M+3 drops urease enzyme had the highest absorbance compared to electrolyte solutions with phosphate buffer and urea content. Likewise, the FTIR results showed the same thing where phosphate buffer solution was 0.001 M pH 7.5 + KCl 0.001 M + urea 0.001 M + 3 drops urease enzyme had the highest% T (transmission) pattern of phosphate buffer solution and urea. The urease enzyme in this study functions as a catalyst. Based on UV-Vis and FTIR characterization, it was concluded that the phosphate buffer solution of 0.001 M pH 7.5+KCl 0.001 M + urea 0.001 M + 3 drops of urease enzyme was the best.

scholarly journals Characterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS, FTIR and XRD analysis

2022 ◽  
Vol 8 (1) ◽  
pp. 103-108
Author(s):  
Abd Hakim S
Keyword(s):  
Enzyme Immobilization ◽  
Xrd Analysis ◽  
Indicator Electrode ◽  
Urease Enzyme

This study aims to characterize the tungsten-urea analyte indicator electrode. The method used is biosensor potentiometry with urease enzyme immobilization technique. This indicator electrode was coated with PVA-enzyme coated with glutaraldehyde (GA) 2.9% coated with PVC-KTpClPB- o-NPOE with o-NPOE variation of 61% and 66%. Characterization of coated indicator electrodes using SEM-EDS, FTIR and XRD analysis. A1-4 61% indicator electrode sample coated PVA-enzyme 1x coated with glutaraldehyde (GA) 2.9% 1x coated PVC-KTpClPB- o-NPOE 1x, with o-NPOE 61%. A3-4 61% indicator electrode sample coated PVA-enzyme 3x coated with glutaraldehyde (GA) 2.9% 1x coated PVC-KTpClPB- o-NPOE 1x, with o-NPOE 61%. Likewise, the reasoning of samples A1-4 66% and A3-4 66%. There are four indicator electrodes made with the notation A1-4 61%, A1-4 66%, A3-4 61% and A3-4 66%. The best results were obtained at the indicator electrode sample A1-4 61%, contributing to the urea sensor of the potentiometer cell

Synthesis and Characterization of SiO2/ZnO Core-Shell Spheres

2011 ◽  
Vol 320 ◽  
pp. 140-145
Author(s):  
Yung Kuan Tseng ◽  
Pei Han Wang ◽  
Shun Lung Su
Keyword(s):  
Uv Light ◽  
Spherical Surface ◽  
Sol Gel ◽  
Xrd Analysis ◽  
Core Shell ◽  
Visible Absorption ◽  
Stöber Method ◽  
Eds Analysis ◽  
Uv Visible

This Study applies to Stöber method for synthesis an characterization of even-sized SiO2micrometer sphere; and then applies to sol-gel method for encapsulation for one layer of ZnO at SiO2spherical surface after regarded it as core so that synthesis SiO2/ZnO core-shell spheres successfully. And observe morphology by FE-SEM and crossection by TEM. The TEM specimen were cut by two-beam FIB. Besides, EDS, XRD and UV-Visible spectroscopy were using for the analysis of composition, crystallization structure, and absorption spectrum, respectively. Experimental results showed that, SiO2spherical surface became smooth after deposition for zinc oxide. The specimen made by FIB more obviously was observed for core-shell distribution by TEM. EDS analysis made us clearly see shell signal with ZnO content, but core signal only is SiO2content. XRD analysis indicated its core-shell sphere signal possessing ZnO content, but UV-Visible absorption spectrometer displayed ZnO has been absorbed in range of UV light, in contrast, it is not absorbed in range of visible light.

scholarly journals Optical Characterization of NiO Doped Fe2O3

2015 ◽  
Vol 45 ◽  
pp. 50-58 ◽  
Author(s):  
Sami Salmann Chiad
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Energy Gap ◽  
Optical Characterization ◽  
Skin Depth ◽  
Spray Pyrolysis Method ◽  
Pyrolysis Method ◽  
Uv Visible ◽  
Absorbance Spectra

In this paper, the Fe2O3 thin film were prepared with various ratios doping of NiO by spray pyrolysis method on glass substrate temperature 400 °C. The initial solution was including a 0.1 M/L for both NiCl2 and FeCl3 diluted with redistilled water and a few drops of HCl. The effect of NiO-doping on optical properties were studied. UV-Visible spectrophotometer in the range of (300-900) nm used to determine absorbance spectra. The transmittance increased with increasing NiO content in NiO:Fe2O3 thin films, same behavior of extinction coefficient and skin depth. The energy gap increased from 2.45 eV before doping to 2.86 eV after 3% NiO-doping. While the reflectance, absorption coefficient, and refractive index are decreased with increasing NiO content in Fe2O3 thin films.

EPR characterization of cellulose triacetate fibers used for enzyme immobilization

1981 ◽  
Vol 6 (2) ◽  
pp. 129-141 ◽  
Author(s):  
S. Gulinelli ◽  
E. Mantovani ◽  
A. Zanobi
Keyword(s):  
Enzyme Immobilization ◽  
Cellulose Triacetate

pH Influences on Optical Absorption of Anthocyanin from Black Rice as Sensitizer for Dye Sensitized Solar Cell TiO2 Nanoparticles

2016 ◽  
Vol 864 ◽  
pp. 154-158
Author(s):  
Mariya Al Qibtiya ◽  
Eka Cahya Prima ◽  
Brian Yuliarto ◽  
Suyatman
Keyword(s):  
Solar Cell ◽  
Optical Absorption ◽  
Dye Sensitized Solar Cell ◽  
Black Rice ◽  
X Ray ◽  
Dye Sensitized ◽  
Current Voltage ◽  
Average Grain Size ◽  
Uv Visible

Natural dyes extracted from black rice are used as sensitizer for dye sensitized solar cell. The anthocyanin extracted with various pH in acidic and neutral coditions. Preparation of fotolectrode TiO2 film using doctor blade method and resulting average grain size 33,9 nm using X-Ray Diffractometer. Characterization of morphology and cross-section film TiO2 is confirmed by Scanning Electron microscopy (SEM). Optical absorption using UV-Visible Spectroscopy to obtain spectrum absorbance of anthocyanin in various pH. The current-voltage (J-V) characterization shows the performance DSSC have a match relation to the optical absorption. The best absorption of anthocyanin obtained at pH 6 as well as conversion efficiency reaches 2.26% at this pH condition.

scholarly journals Identification and Characterization of α-Glucosidase Inhibition Flavonol Glycosides from Jack Bean (Canavalia ensiformis (L.) DC

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2481
Author(s):  
Anita M. Sutedja ◽  
Emiko Yanase ◽  
Irmanida Batubara ◽  
Dedi Fardiaz ◽  
Hanifah N. Lioe
Keyword(s):  
2D Nmr ◽  
The Other ◽  
Inhibition Activity ◽  
Jack Bean ◽  
Canavalia Ensiformis ◽  
Kaempferol Glycosides ◽  
Uv Visible ◽  
First Time ◽  
Identification And Characterization

Although the intake of jack bean (Canavalia ensiformis (L.) DC.), an underutilized tropical legume, can potentially decrease the risk of several chronic diseases, not much effort has been directed at profiling the polyphenolics contained therein. Hence, this work aimed to identify and quantify the dominant jack bean polyphenolics, which are believed to have antioxidant and other bioactivities. Four major compounds were detected and identified as kaempferol glycosides with three or four glycoside units. Their structures were established based on UV-visible, 1D, 2D NMR, and HR-ESI-MS analyses. Specifically, kaempferol 3-O-α-l-rhamnopyranosyl (1→6)- β-d-glucopyranosyl (1→2)-β-d-galactopyranosyl-7-O-[3-O-o-anisoyl]-α-l-rhamnopyranoside was detected for the first time, while the other three compounds have already been described in plants other than jack bean. This new compound was found to have a higher α-glucosidase inhibition activity compared to acarbose.

scholarly journals Isolasi dan Karakterisasi Bakteri Penghasil Etanol dari Lingkungan Industri Arak Bali di Desa Merita dan Tri Eka Buana, Karangasem-Bali

2020 ◽  
Vol 8 (2) ◽  
pp. 279
Author(s):  
Azis Akbar Hakim ◽  
I M. Mahaputra Wijaya ◽  
Ida Bagus Wayan Gunam
Keyword(s):  
Fermentation Process ◽  
Visible Spectrophotometry ◽  
Gram Positive Bacteria ◽  
Production Location ◽  
Coconut Tree ◽  
Isolation And Characterization ◽  
Exposure Method ◽  
Sucrose Medium ◽  
Uv Visible

The purpose of this research is isolation and characterization to find potential bacteria which can produce the most optimal ethanol from the Arak Bali industry in Karangasem Regency, Bali. Bacteria were isolated by exposure method in open air using selective media Zymomonas Sucrose Medium (ZSMA) with the addition of nystatin as much as 0.18 g / L as an antifungal then samples were taken at three different points in one Arak Bali production location, namely the distillation place, the fermentation room for roomie, and the place of taking coconut juice under the coconut tree and the variation of time is 15, 30, and 60 minutes of exposure. Gas checking is done on the bacteria obtained to select its ability to produce ethanol. The results of the scanning of 11 best isolates using UV-visible spectrophotometry were fermented on 500 mL ZSM media for 10 days. BM1-CP14 is the best isolate to produce total ethanol of 15.33 mL through the fermentation process. The results of the characterization of BM1-CP14 isolates were Gram-positive bacteria in the form of bacilli, anaerobic and non-motile bacteria. The results showed that bacteria isolated from open-air also can produce ethanol. Keyword: ethanol, Arak Bali, airborne bacterial exposure, isolation, characterization, UV-Visible spectrophotometry

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