scholarly journals Sensitive spectrofluorimetric and spectrophotometric methods for determination of sparfloxacin in pharmaceuticals

2017 ◽  
Vol 56 (2) ◽  
Author(s):  
Jasmin Shah ◽  
M. Rasul Jan ◽  
Inayatullah . ◽  
Sultan Shah
Keyword(s):  
Acidic Medium ◽  
Heating Temperature ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision ◽  
Spectrofluorimetric Method ◽  
Recovery Percentage ◽  
The Common

Two simple, sensitive and accurate spectrofluorimetric and spectrophotometric methods have been developed for the determination of sparfloxacin in bulk and pharmaceutical preparations. The proposed methods were based on oxidation of sparfloxacin with Ce (IV) in acidic medium. The spectrophotometric method involved the measurement of unconsumed Ce (IV) concentration at 315 nm. The spectrofluorimetric method based on the measurement of reduced fluorescent Ce (III) at 352 nm after excitation at 250 nm. Different variables affecting the reaction such as concentration and volume of cerium (IV), type and concentration of acidic medium, heating temperature and time were carefully studied and optimized. Under the optimum conditions, linear relationship in the range of 0.02-0.2 μg mL<sup>-1</sup> and 0.02-0.1 μg mL<sup>-1</sup> were obtained using spectrophotometric and spectrofluorimetric methods, respectively. No interferences were observed from the common formulations excipients present in the dosage form of the drug. The proposed methods were successfully applied to the analysis of the investigated drug in pure and pharmaceutical formulations with good accuracy and precision. The recovery percentage ranged from 93-102 ± 1.73-2.66%. The precision of the methods were good; RSD ≤ 2.55%.

scholarly journals Spectrophotometric Investigations of the Assay of Physiologically Active Catecholamines in Pharmaceutical Formulations

2002 ◽  
Vol 85 (6) ◽  
pp. 1288-1292 ◽  
Author(s):  
Basavaraj S Nagaralli ◽  
Jaldappa Seetharamappa ◽  
Mahaveer B Melwanki ◽  
Kunabevu C Ramesh ◽  
Jathi Keshavayya
Keyword(s):  
Statistical Analysis ◽  
Pharmaceutical Preparations ◽  
Correlation Coefficients ◽  
Pharmaceutical Formulations ◽  
Dosage Forms ◽  
Spectrophotometric Methods ◽  
Dopamine Hydrochloride ◽  
Beer’S Law ◽  
The Common

Abstract Two simple, sensitive, and accurate spectrophotometric methods are proposed for the determination of levodopa (LD), methyldopa (MD), dopamine hydrochloride (DP), and pyrocatechol (PC) in pure and pharmaceutical preparations. The methods are based on measurement of the absorbances of tris( o-phenanthroline)iron(II) (method A) and tris(bipyridyl)iron(II) (method B) obtained by the oxidation of the catecholamines by iron(III) in the presence of 1,10-phenanthroline and 2,2′-bipyridyl at 510 and 522 nm, respectively. The absorbances were found to increase linearly with increases in the concentrations of the catecholamines, results which were corroborated by the calculated correlation coefficients (0.9990–0.9996). Beer's law was valid over the concentration ranges of 0.04–0.6, 0.06–0.75, 0.06–0.65, and 0.05–0.70 μg/mL in method A and 0.02–1.0, 0.04–1.3, 0.05–1.0, and 0.06–1.1 μg/mL in method B for PC, MD, LD, and DP, respectively. The common excipients and additives did not interfere in their determinations. The proposed methods were successfully applied to the assay of LD, MD, and DP in various dosage forms. The results were validated by statistical analysis.

Colorimetric Determination of Amoxicillin in Pure and some Pharmaceutical Formulations Via Reaction with Potassium Permanganate as Oxidant Reagent

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Abbas Shebeeb Al-kadumi ◽  
Sahar Rihan Fadhel ◽  
Mohammed Abdullah Ahmed ◽  
Luma Amer Musa
Keyword(s):  
Potassium Permanganate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Atomic Emission ◽  
Basic Medium ◽  
Photometric Method ◽  
Colorimetric Determination ◽  
Spectrophotometric Methods ◽  
Label Claim

We proposed two simple, rapid, and convenient spectrophotometric methods are described for the determination of Amoxicillin in bulk and its pharmaceutical preparations. They are based on the measurement of the flame atomic emission of potassium ion (in first method) and colorimetric determination of the green colored solution for manganite ion at 610 nm formed after reaction of Amoxicillin with potassium permanganate as oxidant agent (in the second method) in basic medium. The working conditions of the methods were investigated and optimized. Beer's law plot showed a good correlation in the concentration range of 5-45 μg/ml. The detection limits and relative standared deviations were (2.573, 2.814 μg/ml) (2.137, 2.498) for the flame emission photometric method and (1.844, 2.016 μg/ml) (1.645,1.932) for colorimetric methods for capsules and suspensions respectively. The methods were successfully applied to the determination of Amoxicillin in capsules and suspensions, and the obtained results were in good agreement with the label claim. No interference was observed from the commonly encountered additives and expectancies.

scholarly journals Ion Association Methods for the Determination of Fexofenadine in Pharmaceutical Preparations

2005 ◽  
Vol 2 (3) ◽  
pp. 199-202 ◽  
Author(s):  
L. D. Srinivas ◽  
P. Ravi Kumar ◽  
B. S. Sastry
Keyword(s):  
Pharmaceutical Preparations ◽  
Ion Association ◽  
Pharmaceutical Formulations ◽  
Bulk Sample ◽  
Acid Group ◽  
Carboxylic Acid Group ◽  
Spectrophotometric Methods ◽  
Safranin O ◽  
Association Complex

Two simple and sensitive Visible spectrophotometric methods (A and B) for the determination of Fexofenadine (FEX) in bulk sample and pharmaceutical formulations are described. Methods A and B are based on the formation of ion-association complex involving carboxylic acid group of FEX and the basic dyes, Safranin-O (SFN-O, method A), methylene blue (MB, method B). The results obtained in the above two methods are reproducible and are statistically validated and found to be suitable for the assay of Fexofenadine in bulk and its pharmaceutical formulations.

scholarly journals Simple Methods for the Spectrophotometric Determination of Carvedilol

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Divya N. Shetty ◽  
B. Narayana
Keyword(s):  
Charge Transfer ◽  
Complex Formation ◽  
Confidence Level ◽  
Charge Transfer Complex ◽  
Condensation Reaction ◽  
Pharmaceutical Preparations ◽  
Molar Absorptivity ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision

Two simple spectrophotometric methods are described for the determination of carvedilol (CAR). Method A is the condensation reaction of CAR with p-dimethylaminobenzaldehyde (PDAB), and the reaction mixture exhibits maximum absorbance at 601 nm. Method B is based on the charge transfer complex formation of CAR with p-chloranil; the color developed is measured at 662 nm. The calibration graphs are found to be linear over 50.00–250.00 and 20.00–100.0 μg mL−1 with molar absorptivity values of 0.92×103 and 0.257×104 L mol−1cm−1 for CAR-PDAB and CAR-p-chloranil, respectively. Statistical comparisons of the results are performed with regard to accuracy and precision using Student’s t-test and F-test at 95% confidence level. The methods are successfully employed for the determination of CAR in pharmaceutical preparations, and the results agree favorably with the reference and proposed methods.

scholarly journals Indirect Spectrophotometric Methods for the Determination of Tadalafil

2015 ◽  
Vol 1 (2) ◽  
pp. 11 ◽  
Author(s):  
Safwan Mohammad Fraihat
Keyword(s):  
Oxidation Reaction ◽  
Indigo Carmine ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Blue Dye ◽  
Reaction Products ◽  
Reaction Conditions ◽  
Factors Affecting ◽  
Spectrophotometric Methods

Two spectrophotometric methods were developed for the determination Tadalafil in pharmaceutical preparations. The methods are based on the oxidation reaction with known excess amount of Ce(IV) and estimation of the unreacted amount using Indigo carmine dye (Method A) and in Methylene blue dye  (Method B). the factors affecting the reaction conditions were studied and the absorbance of absorbance of the oxidation reaction products were monitored at 610 and 600 nm for methods A and B respectively. Beer's law is obeyed in the concentration ranges 11 to 50 and 10 to 55 ppm, the limits of detection and quantification are reported. The proposed method was applied to the determination of the drug in pharmaceutical formulations and the results demonstrated that the method is equally accurate, precise and reproducible as the official methods. The validity of method was established by recovery studies with satisfactory results.

scholarly journals Spectrofluorometric Determination of Ketorolac Tromethamine Via Its Oxidation with Cerium(IV) in Pharmaceutical Preparations and Biological Fluids

2007 ◽  
Vol 90 (4) ◽  
pp. 941-947 ◽  
Author(s):  
Manal Eid ◽  
Amina El-Brashy ◽  
Fatma Aly ◽  
Wael Talaat
Keyword(s):  
Biological Fluids ◽  
Sulfuric Acid Concentration ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Official Method ◽  
Eye Drops ◽  
Ketorolac Tromethamine ◽  
Accuracy And Precision ◽  
Spectrofluorometric Determination

Abstract A simple and sensitive fluorometric method for determination of ketorolac tromethamine was studied. The method depends on oxidation of the drug with cerium(IV) and subsequent monitoring of the fluorescence of the induced cerium(III) at em 365 nm after excitation at 255 nm. Different variables affecting the reaction conditions, such as the concentrations of cerium(IV), sulfuric acid concentration, reaction time, and temperature, were carefully studied and optimized. Under the optimum conditions, a linear relationship was found between the relative fluorescence intensity and the concentration of the investigated drug in the range of 0.10.8 g/mL. No interferences could be observed from the excipients commonly present in dosage forms. The proposed method was successfully applied to the analysis of the investigated drug in its pure form, pharmaceutical preparations, and biological fluids with good accuracy and precision. The recoveries for pharmaceutical formulations ranged from 99.8101.0 0.6% for tablets, 98.5101.0 1.0% for ampoules, and 99.0100.5 0.7% for eye drops. The results obtained by the proposed method were satisfactory compared with those obtained by the official method. The recoveries for biological fluids were 99.1100.4 0.7 and 99.0100.0 0.5% for plasma and urine, respectively.

scholarly journals New Application of Chromotropic Acid for the Determination of Some Sulphonamides in their Pharmaceutical Preparations

2002 ◽  
Vol 70 (1) ◽  
pp. 49-55
Author(s):  
Abou-Attia Fekria M. ◽  
Issa Y.M. ◽  
El Reis M.A. ◽  
Aly F.A. ◽  
Abd El- MoetY M.
Keyword(s):  
Azo Dye ◽  
Good Accuracy ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Extraction Process ◽  
Molar Absorptivity ◽  
Chromotropic Acid ◽  
Time Consumption ◽  
Accuracy And Precision

The formation of the azo-dye using chromotropic acid as a coupling agent was applied to the determination of five sulphonamides. The spectrophotometric studies as well as microanalysis of the studied sulphonamides- chromotropic acid azo dyes revealed the existence of the 1:1 coupling product. The absorbance of the formed azo dye is measured at 510 nrn, and has a large molar absorptivity (ε = 2.87 - 3.29 × 104 1 mol−1cm−1). Beer's law was obeyed over the concentration range 0.5-9.0 µg ml−1. The assay results of pharmaceutical formulations showed good accuracy and precision over the concentration range used. The proposed method avoids time-consumption, extraction process and temperature control.

scholarly journals Validated Extractive Spectrophotometric Method for Determination of Domperidone in Pharmaceutical Formulations

2015 ◽  
Vol 17 (1) ◽  
pp. 25-31
Author(s):  
Jasmin Shah ◽  
M Rasul Jan ◽  
Muhammad Tariq Shah
Keyword(s):  
Acidic Medium ◽  
Pharmaceutical Formulations ◽  
Pharmaceutical Journal ◽  
Bromothymol Blue ◽  
Hplc Method ◽  
Ion Pair ◽  
Bromophenol Blue ◽  
Spectrophotometric Methods ◽  
New Methods

Simple, precise and sensitive extractive spectrophotometric methods have been developed for the determination of domperidone in pharmaceutical formulations. The new methods involve the formation of colored extractable ion pair complexes of the drug with bromothymol blue (BTB) and bromophenol blue (BPB) in acidic medium. The effects of various parameters like pH, reagent concentration and shaking time were studied. The extracted complexes of domperidone showed maximum absorbance at 410 nm with BTB and at 415 nm with BPB dye. The stiochiometry of the reaction between domperidone, BTB and BPB was found to be 1: 4. Domperidone was found to obey Beer’s law in the concentration ranges of 0.6-35 ?g/ml, 1-30 ?g/ml with BTB and BPB, respectively. The method has been applied successfully for the determination of domperidone in commercial tablets and suspension samples. The results obtained by the proposed methods were validated statistically and compared with the official HPLC method. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22310 Bangladesh Pharmaceutical Journal 17(1): 25-31, 2014

scholarly journals Optimization of Two Flow-Injection Spectrophotometric Methods for the Determination of Indapamide in Pharmaceutical Dosage Forms

2005 ◽  
Vol 88 (4) ◽  
pp. 1148-1154 ◽  
Author(s):  
Juan C Rodríguez ◽  
Julia Barciela ◽  
Sagrario García ◽  
Carlos Herrero ◽  
Rosa M Peña
Keyword(s):  
Flow Injection ◽  
Screening Method ◽  
Pharmaceutical Preparations ◽  
Dosage Forms ◽  
Response Surfaces ◽  
Subsequent Formation ◽  
Pharmaceutical Dosage Forms ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision

Abstract Multivariate experimental design has been used to optimize 2 flow-injection spectrophotometric methods for the determination of indapamide in pharmaceutical dosage forms, both pure and commercial tablets. The methods are based on the oxidation of this drug with iron (III) in acidic medium and the subsequent formation of an intensive orange-red complex between the liberated iron (II) and 2,2′-bipyridyl or 1,10-phenanthroline reagents. Plackett-Burman designs were applied as a screening method to evaluate the most significant factors with few experiments. Central composite 23+ star designs were performed to evaluate the response surfaces. The methods have been fully validated and were applied successfully to the determination of indapamide in pure and pharmaceutical forms with good accuracy and precision. Therefore, the 2 proposed procedures are simple, inexpensive, and rapid flow methods for the routine determination of indapamide in pharmaceutical preparations.

scholarly journals Simple Spectrophotometric Methods for the Determination of Sulfamethaxazole in Pharmaceuticals Using Folinciocalteau and Orcinol as Reagents

2009 ◽  
Vol 6 (2) ◽  
pp. 357-360 ◽  
Author(s):  
G. Vijaya Raja ◽  
C. Bala Sekaran ◽  
D. Winnie Teja ◽  
B. Madhuri ◽  
B. Jayasree
Keyword(s):  
Sodium Carbonate ◽  
Sodium Nitrite ◽  
Acidic Medium ◽  
Biological Fluids ◽  
Pharmaceutical Preparations ◽  
Phosphomolybdic Acid ◽  
Dosage Forms ◽  
Maximum Absorption ◽  
Spectrophotometric Methods

Two simple, sensitive and reproducible spectrophotometric methods (Method A and Method B) were developed for the determination of sulfamethaxazole in bulk and in dosage forms. Method A is based reduction of phosphomolybdic acid present in Folin Ciocalteau reagent by the drug sulfamethaxazole in the presence of sodium carbonate to form a blue colored chromogen having maximum absorption at 760 nm. Method B is based on the diazotization of the drug by sodium nitrite in acidic medium at 5°C followed by coupling with orcinol to form yellow colored species (λmax390 nm). Beer’s law is obeyed in the range of 5-25 μg/mL for method A and 2-10 μg/mL for method B. Results of analysis were validated statistically and by recovery studies. These methods are successfully employed for the determination of sulfamethaxazole in various pharmaceutical preparations and biological fluids.

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