scholarly journals Gross and Histomorphological Study of the Ovary and Oviduct of Turkey Hen with Especial Emphasis on the Sperm-Host Gland

2021 ◽  
Vol 45 (1) ◽  
pp. 1-8
Author(s):  
Mohammad Rafiqul Islam ◽  
Imam Hasan ◽  
Nure Zannat Monisha ◽  
Marya Afrin
Keyword(s):  
Lamina Propria ◽  
Meleagris Gallopavo ◽  
Proximal Part ◽  
Mature Female ◽  
Columnar Epithelium ◽  
Meat Production ◽  
Growth Strategies ◽  
Outer Cortex ◽  
Tissue Samples ◽  
Sperm Storage Tubules

Turkey bird is one of the popular poultry species which is reared primarily for meat production and considered as one of the major sources of animal protein. With such importance of this species, this study was designed to investigate the gross and histomorphology of the ovary and oviduct with especial emphasis on sperm-host glands of the turkey hen involving ten mature female turkeys (Meleagris gallopavo). The present study highlighted the distribution pattern of sperm-host glands (SHGs) in the oviduct of turkey hen that has a potential role in producing a fertile egg in poultry industries. The oviduct of turkey consists of the infundibulum, magnum, isthmus, uterus, and vagina which are sole distributors for making nutrition enriched egg. The tissue samples were collected from the ovary, different segments of the oviduct and especially uterovaginal junction (UVJ) and infundiomagnal junction of the oviduct. The ovaries and the oviducts were dissected and fixed in Bouins solution and processed for a light microscopic study. Histologically, the left ovary of turkey consisted of an outer cortex and inner medulla, with different stages of follicles. In all areas of the oviduct except the infundibulum and vagina, the tunica mucosa epithelium was lined with ciliated pseudo stratified columnar epithelium, and the lamina propria-submucosa contained branched tubular glands. Sperm-storage tubules were observed in the uterovaginal junction and infundibulo-magnum junction. These tubules were mostly branched, slightly coiled and extended into the lamina propria from the bases of the mucosal folds. These glands had proximal and distal parts; the proximal part was lined by pseudostratified columnar epithelium and distal part by non-ciliated simple columnar epithelium. The number of sperm host glands was more at uterovaginal junction than infundibulomagnal junction. The sperm-host glands might play a functional role in the storage and release of spermatozoa from the SHGs in response to oviposition or ovulation. The results would help poultry scientists and farmers in developing effective disease control and growth strategies.

scholarly journals Assessment of the morphological development of the caecal tonsil in turkey (Meleagris gallopavo)

1970 ◽  
Vol 8 (1) ◽  
pp. 1431-1437
Author(s):  
Okechukwu Nnadozie ◽  
Ekele Ikpegbu ◽  
Uchenna Callitus Nlebedum ◽  
Isaiah Agbakwuru
Keyword(s):  
Connective Tissue ◽  
Plasma Cells ◽  
Gradual Increase ◽  
Meleagris Gallopavo ◽  
Proximal Part ◽  
Goblet Cells ◽  
Lymphoid Cells ◽  
Columnar Epithelium ◽  
Morphological Development ◽  
Nodular Structure

The post hatch (PH) development of the caecal tonsil in turkey (Meleagris gallopavo) was studied from day (D) 1 post-hatch to D 140. The tonsils appeared as nodular structure on the surface of the proximal part of the caecal base. The caecal wall of all ages was composed of four histological layers. The mucosa consisted of simple columnar epithelium with goblet cells. At D 1 PH the tonsil was composed of minute aggregates of proliferating lymphoid cells. By D 7, Leiberkuhn glands were observed in the stroma of the tonsil, and at D 28, nodules packed with lymphocytes and some plasma cells were observed. Between D 28 and D 133, there were gradual increase in dimension of the tonsils and proliferation of lymphatic nodules. At D 140, there was depletion of lymphatic nodules especially at the apical regions bordering the caecal lumen, and increase in thickness of the intervening connective tissue was very obvious.Key words: caecal tonsil, turkey (Meleagris gallopavo ), morphology, post-hatch, development

scholarly journals Spermathecae of the mangrove crab Ucides cordatus: a histological and histochemical view

2007 ◽  
Vol 87 (4) ◽  
pp. 903-911 ◽  
Author(s):  
Bruno Sampaio Sant'Anna ◽  
Marcelo Antonio Amaro Pinheiro ◽  
Márcia Mataqueiro ◽  
Fernando José Zara
Keyword(s):  
Seminal Fluid ◽  
Proximal Part ◽  
Economic Importance ◽  
Columnar Epithelium ◽  
Female Reproductive System ◽  
Collagen Fibres ◽  
Ucides Cordatus ◽  
Sudan Black B ◽  
Mangrove Crab ◽  
Commercially Important

Ucides cordatus is the most commercially important mangrove crab in Brazil. In spite of its economic importance, there are few studies of its reproduction, in particular the female reproductive system. The present study describes the histology and histochemistry of the spermathecae of U. cordatus. Adult females were caught monthly from July 2004 through June 2005, at Iguape, State of São Paulo. The crabs were anaesthetized, and their spermathecae removed and fixed in Davidson's fluid, following the histological routine for paraffin. The slides were stained with HE, xylidine Ponceau, PAS, alcian blue (pH 1.0 and 2.5), Sudan black B and picrosirius-haematoxylin. Histologically, the spermathecae possesses a capsule of conjunctive tissue, rich in collagen fibres, which surrounds the secretory columnar epithelium. In the lumen, individual sperm packets are not observed; the spermatophores are intermixed with the seminal fluid and secretions of the spermathecae itself. A large proportion of the free spermatozoids and spermatophores are arranged in homogeneous masses in the proximal part of the spermathecae. The secretion produced by the columnar epithelium appears to promote the movement of the gametes to the fertilization chamber, in a ventral position, allowing fertilization of the oocytes. Histochemically, the secretion produced by the columnar epithelium was strongly positive for neutral polysaccharides, positive for acid polysaccharides, and weakly positive for proteins and lipids. This secretion forms a glycoprotein matrix which is associated with maintenance of the spermatophores, which can remain stored for long periods.

scholarly journals Cathaemasia hians infection in Black stork in Slovakia: morphological and histopathological study

2015 ◽  
Vol 52 (4) ◽  
pp. 316-322 ◽  
Author(s):  
A. Königová ◽  
G. Hrčková ◽  
L. Molnár ◽  
P. Major ◽  
M. Várady
Keyword(s):  
Inflammatory Response ◽  
Lamina Propria ◽  
Histopathological Examination ◽  
Fibrous Tissue ◽  
Mechanical Damage ◽  
Histopathological Study ◽  
Intermediate Hosts ◽  
Tissue Samples ◽  
Morphological Alterations ◽  
Black Stork

SummaryCathaemasia hians is an obligate trematode parasite of Black storks that are on the List of protected birds in Europe. In the present study, adult trematodes were isolated from the Black stork post mortem and morphological study revealed C. hians species. In total, 10 worms were found in the oesophagus and the ventriculus of the bird. Histopathological examination of the tissue samples of oesophagus, proventriculus and ventriculus was performed on paraffin sections using a set of staining procedures. The sporadic lesions were seen in the tela submucosa of oesophagus containing connective tissue mast cells, eosinophils and heterophils and some foci were surrounded by the fibrous tissue. In addition, a few inflammatory nodules had larval-like material inside, probably being of the same species. There were no visible morphological alterations in the epithelial layer of lamina propria mucosae of proventriculus, rich in goblet cells as well as in the tela submucosa. Majority of trematodes were localized in the ventriculus, where the lamina propria mucosae was damaged or disrupted sporadically. In these sites, in the tela submucosa, a various food-originated inorganic/organic material and eggs of C. hians were deposited, stimulating a weak inflammatory response. Nodules containing larvae were not observed in any of ventriculus tissue layers. This study demonstrated, for the first time, infection with adults of C. hians trematode in the Black stork nesting in Slovakia. The presence of larvae and eggs in the tissues of the upper gastrointestinal tract of bird was associated with mild inflammatory response but feeding behaviour of adult worms in the ventriculus probably contributed to the enhanced susceptibility of the lamina propria mucosae to mechanical damage by inorganic material. Although larval stages have not yet been documented in the intermediate hosts in Slovakia, our report indicates that the life cycle of C. hians might occur in Central Europe.

scholarly journals Phenotypic and functional characterisation of myofibroblasts, macrophages, and lymphocytes migrating out of the human gastric lamina propria following the loss of epithelial cells

Gut ◽  
1999 ◽  
Vol 44 (3) ◽  
pp. 323-330 ◽  
Author(s):  
K C Wu ◽  
L M Jackson ◽  
A M Galvin ◽  
T Gray ◽  
C J Hawkey ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Basement Membrane ◽  
Lamina Propria ◽  
Surface Epithelium ◽  
Prostaglandin E ◽  
Tissue Samples ◽  
Membrane Pores ◽  
Functional Characterisation ◽  
Polymerase Chain ◽  
Cox 1

BACKGROUNDThe basement membrane of human colonic mucosa contains numerous discrete pores. We have recently shown that following loss of the surface epithelium, many cells migrate out of the colonic lamina propria via basement membrane pores.AIMSTo characterise cells migrating out via basement membrane pores of the human gastric lamina propria, following loss of the surface epithelium.METHODSFresh human gastric mucosal samples were completely denuded of epithelial cells and placed in culture. Tissue samples were studied by electron microscopy (EM) and cells by EM, FACS analysis, immunohistochemistry, and reverse transcription polymerase chain reaction (RT-PCR).RESULTSEM showed numerous discrete pores (0.65–8.29 μm in diameter) in the subepithelial basement membrane. During culture of mucosal samples denuded of epithelial cells, lymphocytes, macrophages, and myofibroblasts migrated out of the lamina propria via the basement membrane pores. The lymphocytes were predominantly CD45RO+ and CD69+ T cells. Macrophages were shown to express cyclooxygenase (COX) 1 and 2 enzymes. Myofibroblasts were established in culture and, despite prolonged culture and passage, retained their phenotype. They expressed mRNA and protein for COX 1 and 2 enzymes and their release of prostaglandin E2 was inhibited by selective COX 1 and 2 inhibitors.CONCLUSIONSLamina propria cells migrating out of cultured denuded gastric mucosal samples have been characterised phenotypically and functionally. Such cells would be suitable for studies of their interactions with epithelial cells and also with Helicobacter pylori and its products.

scholarly journals Histochemical investigation of the esophagus of the Wild bronze turkey (Meleagris gallopavo)

2019 ◽  
Vol 17 (4) ◽  
pp. 308-311 ◽  
Author(s):  
D. Yovchev ◽  
G. Penchev
Keyword(s):  
Lamina Propria ◽  
Alcian Blue ◽  
Meleagris Gallopavo ◽  
Histochemical Investigation ◽  
Histological Features ◽  
Pas Reaction ◽  
Glandular Cells ◽  
Pas Staining ◽  
Thoracic Part ◽  
Tunica Muscularis

The aim of the present study was to investigate the thoracic part of the esophagus in the bronze turkey, using Masson’s trichrome stain and Alcian blue-PAS staining. Thirty-six clinically healthy bronze turkeys (eighteen males and eighteen females) were studied. The groups of the birds were at age 1, 7, 14, 28, 35 and 49 days. Each group consisted of three male and three female birds. The histological features of the organ were similar in the studied groups. It consisted of tunica mucosa, tunica submucosa, tunica muscularis, and tunica serosa. There were no specifics in the organ regarding the sex and the age of the birds. The esophageal glands in all of the studied groups, demonstrated intensive PAS and AB reaction, because of the mucous, produced by the epithelial glandular cells. Lamina propria exhibited a weak PAS reaction, visible in all ages, either in males, either in females.

scholarly journals Peyer's patch eosinophils: identification, characterization, and regulation by mucosal allergen exposure, interleukin-5, and eotaxin

Blood ◽  
2000 ◽  
Vol 96 (4) ◽  
pp. 1538-1544
Author(s):  
Anil Mishra ◽  
Simon P. Hogan ◽  
Eric B. Brandt ◽  
Marc E. Rothenberg
Keyword(s):  
Gastrointestinal Tract ◽  
Lamina Propria ◽  
Allergen Exposure ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Outer Cortex ◽  
Interleukin 5 ◽  
Marked Accumulation ◽  
Low Levels

The gastrointestinal immune system is traditionally thought to be composed of lymphocytes located within Peyer's patches and the lamina propria. We have recently reported that eosinophils also reside in the gastrointestinal tract during healthy states, in particular, within the lamina propria, and that these cells substantially increase after oral allergen exposure. We now demonstrate the presence of eosinophils in Peyer's patches and characterize the signals that regulate the accumulation of eosinophils in Peyer's patches. In contrast to the lamina propria, intestinal Peyer's patches have very low levels of eosinophils under healthy states. However, elevated levels of interleukin-5 (IL-5), generated by transgenic or pharmacologic approaches, result in a dramatic increase in eosinophil levels in Peyer's patches. Most eosinophils are located in the outer cortex and interfollicular regions of the Peyer's patches. To dissect the mechanism of eosinophil trafficking to Peyer's patches, the role of eotaxin was examined. Mice transgenic for IL-5 and genetically deficient in eotaxin were found to have reduced levels of eosinophils in Peyer's patches compared with IL-5-transgenic mice. To prove that eosinophils also traffic to Peyer's patches in wild-type mice, allergic hypersensitivity was induced and Peyer's patches were examined. Exposure to mucosal allergen promoted marked accumulation of eosinophils in Peyer's patches and this process was attenuated in eotaxin-deficient mice. In summary, these data demonstrate that elevated levels of IL-5 and mucosal allergen exposure promote eotaxin-dependent eosinophil trafficking to Peyer's patches. These studies suggest that eosinophils may cooperate with lymphocytes in the development of mucosal immune responses in the gastrointestinal tract.

scholarly journals Peyer's patch eosinophils: identification, characterization, and regulation by mucosal allergen exposure, interleukin-5, and eotaxin

Blood ◽  
2000 ◽  
Vol 96 (4) ◽  
pp. 1538-1544 ◽  
Author(s):  
Anil Mishra ◽  
Simon P. Hogan ◽  
Eric B. Brandt ◽  
Marc E. Rothenberg
Keyword(s):  
Gastrointestinal Tract ◽  
Lamina Propria ◽  
Allergen Exposure ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Outer Cortex ◽  
Interleukin 5 ◽  
Marked Accumulation ◽  
Low Levels

Abstract The gastrointestinal immune system is traditionally thought to be composed of lymphocytes located within Peyer's patches and the lamina propria. We have recently reported that eosinophils also reside in the gastrointestinal tract during healthy states, in particular, within the lamina propria, and that these cells substantially increase after oral allergen exposure. We now demonstrate the presence of eosinophils in Peyer's patches and characterize the signals that regulate the accumulation of eosinophils in Peyer's patches. In contrast to the lamina propria, intestinal Peyer's patches have very low levels of eosinophils under healthy states. However, elevated levels of interleukin-5 (IL-5), generated by transgenic or pharmacologic approaches, result in a dramatic increase in eosinophil levels in Peyer's patches. Most eosinophils are located in the outer cortex and interfollicular regions of the Peyer's patches. To dissect the mechanism of eosinophil trafficking to Peyer's patches, the role of eotaxin was examined. Mice transgenic for IL-5 and genetically deficient in eotaxin were found to have reduced levels of eosinophils in Peyer's patches compared with IL-5-transgenic mice. To prove that eosinophils also traffic to Peyer's patches in wild-type mice, allergic hypersensitivity was induced and Peyer's patches were examined. Exposure to mucosal allergen promoted marked accumulation of eosinophils in Peyer's patches and this process was attenuated in eotaxin-deficient mice. In summary, these data demonstrate that elevated levels of IL-5 and mucosal allergen exposure promote eotaxin-dependent eosinophil trafficking to Peyer's patches. These studies suggest that eosinophils may cooperate with lymphocytes in the development of mucosal immune responses in the gastrointestinal tract.

scholarly journals Histomorphological Description of the Digestive System of Pebbly Fish, Alestes baremoze (Joannis, 1835)

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Nasser Kasozi ◽  
Gerald Iwe Degu ◽  
Julius Mukalazi ◽  
Charles Drago Kato ◽  
Majid Kisekka ◽  
...  
Keyword(s):  
Lamina Propria ◽  
Digestive System ◽  
Anterior Part ◽  
Stomach Content Analysis ◽  
Columnar Epithelium ◽  
Central Vein ◽  
Anterior Portion ◽  
Standard Procedures ◽  
Stratified Squamous Epithelium ◽  
Small Mouth

Histomorphological studies of the digestive system of Alestes baremoze captured from Lake Albert, Uganda, were done using standard procedures. These revealed that A. baremoze has a fleshy-lipped terminal small mouth, large molar, short oesophagus, a three-lobed liver, pouch-like stomach, a nine-fingered caeca, and a long tubular intestine. A stratified squamous epithelium with numerous mucus-secreting cells lined the lips with no taste buds. Stratified squamous epithelia lined the oesophagus in the anterior portion which turned into a columnar epithelium towards the stomach. The lamina propria had numerous tubular glands throughout the entire oesophageal length. The stomach consisted of three distinct regions (cardiac, fundic, and pyloric) with distinguished lamina propria glands. The intestinal mucosa was thrown into villi of varying heights, with the tallest in the anterior part, lined with a simple columnar epithelium with numerous lymphocytes-like infiltrations. Numerous goblet cells appeared in the intestinal lamina epithelialis; these increased uniformly towards the anal opening. The liver was divided into lobules, with a central vein. Hepatocytes were visibly arranged closely, forming irregular cords, and the scattered tubular acinar glands formed the exocrine pancreas (hepatopancreas). Stomach content analysis indicated that the fish eats plankton, mollusks, crustaceans, and insects as the main proportion of its diet.

scholarly journals Morphology of the esophageal mucosa in long-eared owl and eurasian jay

2018 ◽  
Vol 20 (83) ◽  
pp. 200-203
Author(s):  
N. Dyshlyuk
Keyword(s):  
Connective Tissue ◽  
Lamina Propria ◽  
Elastic Fibers ◽  
Esophageal Mucosa ◽  
Muscularis Mucosae ◽  
Loose Connective Tissue ◽  
Smooth Muscle Tissue ◽  
Tissue Samples ◽  
Paraffin Block ◽  
Mucosal Folds

The morphology of the esophageal mucosa in long-eared owl (Asio otus) and Eurasian jay (Garrulus glandarius) has been studied. Samples with exception of the region of the esophageal tonsil were obtained from 3 adult birds of each species. They were studied by light microscopy using classical methods of morphological research. The tissue samples were fixed in 10% neutral buffered formalin and embedded in paraffin block and finally the section were cut at 5–10 µm thickness using sliding microtome and stained with hematoxylin and eosin, according to Van Gizon and Weigert and impregnated with silver nitrate according to Kelemen. The esophageal mucosa consisted of four layers: epithelium, lamina propria, muscularis mucosae, and submucosa. The epithelium was stratified squamous and better developed in the cranial part of the esophagus. The lamina propria was represented by a loose connective tissue with small amount of elastic fibers. In the long-eared owl tightly located numerous small secretory sections of the esophagus glands organised in 1–3, and in branches of large folds up to 6 layers, were found. Their short excretory ducts opened to the surface of the mucosa. The muscularis mucosae was represented by a smooth muscle tissue with bundles of cells directed longitudinally and in some areas appeared to be absent. The submucosa was well developed, and as well as lamina propria was formed by a loose connective tissue with a large number of elastic fibers. In the submucosa of the Eurasian jay secretory departments of large, not tightly located, esophageal mucous glands were found. The esophageal mucosa of this birds formed well-developed folds, which were composed of  all its layers. Their number in the long-eared owl was 20–25 and in the Eurasian jay – 5–9. The shape of folds on the transverse section is not the same: in the long-eared owl it was finger-shaped and leaf-shaped, but in the Eurasian jay it was wedge-shaped. Some large folds in the long-eared owl branched into the secondary mucosal folds. The height of folds in cranial part of the esophagus was larger than in caudal part. In both parts of the esophagus of long-eared owl  the height of the large and middle folds was larger than that in the Eurasian jay. The height of small folds in the Eurasian jay was greater than that in the long-eared owl. In both parts of the esophagus of this birds, the fold’s width at the base exceeded its width at the top. In the Eurasian jay the width of base and top of large, medium and small folds was larger than that in the long-eared owl. The esophageal mucosa was poor on immune formations. They were represented by single accumulations of diffuse lymphoid tissue, and in the Eurasian jay – even by secondary lymphoid nodules.

Assessment of the histological quality of endoscopic biopsies obtained from the canine gastro-esophageal junction

2012 ◽  
Vol 40 (05) ◽  
pp. 318-324 ◽  
Author(s):  
T. Bilzer ◽  
K. Dettmann ◽  
M. Vieth ◽  
M. Münster ◽  
A. Hörauf
Keyword(s):  
Pilot Study ◽  
Lamina Propria ◽  
Squamous Epithelium ◽  
Columnar Epithelium ◽  
Study Endpoint ◽  
Biopsy Specimens ◽  
Endoscopic Biopsies ◽  
Single Use ◽  
A Prospective Study

Summary Objective: In the dog biopsy samples from the gastro-esophageal junction (GEJ) are rarely obtained during routine gastroscopy. The aim of this pilot study was to assess the histological quality of endoscopic biopsies sampled from the canine esophagus and cardia. It was hypothesised that it is possible to sample adequate specimens from these sites. Materials and methods: For this purpose 10 dogs with an indication for gastroscopy were enrolled in a prospective study. Biopsy samples were obtained with standard biopsy forceps for single use exactly from the GEJ thus containing preferably columnar epithelium from the cardia and squamous epithelium from the esophagus, respectively. In every dog the specimens were examined for size, layers and site, respectively. Study endpoint was reached when specimens originated from cardia and esophagus, showing at least epithelium and lamina propria mucosae, and a diameter > 2 mm on the slide, respectively. Results: 72 biopsy specimens (median 7, range 5–10) obtained from the GEJ were examined in 10 dogs. Specimens from the esophagus containing squamous epithelium with lamina propria mucosae were found in 5 of 10 (50.0%) dogs. Specimens from the cardia containing columnar epithelium with lamina propria mucosae were found in 10 of 10 (100.0%) dogs. Four of 10 (40.0%), and 10 of 10 (100.0%) dogs showed at least one specimen > 2 mm on the slide originating from the esophagus, and from the cardia, respectively. Histological quality was found to be adequate in 4 of 10 (40.0%) dogs, showing specimens of adequate size, originating from both esophagus and cardia, and containing at least epithelium and lamina propria mucosae. Conclusion and clinical relevance: The pilot study provides evidence that during routine gastroscopy it is possible to sample endoscopic biopsies from the cardia and with limitations from the esophagus showing a quality adequate for histological examination of the epithelium and the lamina propria mucosae.

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