scholarly journals Some bioactive component analysis of herbal capsules

2021 ◽  
Vol 17 (2) ◽  
pp. 057-062
Author(s):  
Mustafa Yaşar ◽  
Sibel Bayıl Oğuzkan ◽  
Halil İbrahim Uğraş

Since the beginning of the evaluation of plants in terms of human health, the bioactivity properties of plants have been studied in the laboratory and thus standards have been brought to the treatment methods with plants. For this purpose, some bioactive component analyzes of 18 herbal capsules and 2 liquid herbal syrups produced by Naturin Company were performed in our current study. In this context, the total oxidant and antioxidant status of these plant mixtures were evaluated. The free radical capacities of the samples were determined by 1, 1-diphenyl-2-picrylhydrazil (DPPH) method and their DNA protective activities were determined using pBR322 plasmid DNA. Total antioxidant level (TAL) and Total oxidant level (TOL) activities were performed using Rel Assay diagnostic kit. All 3 of the stinging nettle samples showed DNA protective activity. The clean sample containing milk thistle extract also showed DNA Protective activity. In both the syrup samples (My guard and DTX-19) results were detected in the direction of positive protection on DNA. When the DPPH radical scavenging capacity was examined, it was determined that the best results were in juniper, nettle and thistle thistle samples, and the samples with syrup had a very good radical scavenging effect, and all of the samples showed antioxidant activity. This study is a preliminary and supportive study in order to elucidate the properties that can be used as drug active ingredients in traditional medicine, especially in the field of pharmacology.

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Chai-Hyeon Lee ◽  
Ye-Na Park ◽  
Hyeung-Rak Kim ◽  
Sang Gil Lee ◽  
Bohkyung Kim

Abstract Objectives It has been a long time since seaweeds have been used for food ingredients in Asian countries. Recently, a body of research has revealed the health benefits of bioactive compounds in seaweeds, especially their antioxidant capacities. Although numerous seaweeds inhabit in the ocean, only a small percentage has been explored for functional food. Therefore, the purpose of this study was to analyze the antioxidant capacities of various seaweeds grown off the Korean coast to screen for the potential functional food sources. Methods Ten ethanol extracts of Korean seaweeds were provided by the National Marine Biodiversity Institute of Korea, which included Scytosiphon gracilis, Scytosiphon lomentaria, Sargassum muticum, Sargassum confusum, Petrospongium rugosum, Sargassum fusiforme, Petalonia fascia, Sargassum nigrifolium, Ishige foliacea, and Myelophycus simplex. Hundred mg/L of samples in 80% methanol was used to measure their ABTS, DPPH, and superoxide radical scavenging activities. Mg vitamin C equivalent antioxidant capacity (VCEAC)/100 mg was used to express the ABTS and DPPH radical scavenging capacities. For the superoxide radical scavenging capacity, inhibition rate of superoxide radical generation (%) was calculated. Results Among the ten seaweeds, S. nigrifolium and I. foliacea exhibited the most significant radical scavenging capacities. DPPH radical scavenging capacities of I. foliacea and S. nigrifolium were 122.4 mg VCEAC/100 mg and 95.8 mg VCEAC/100 mg, respectively. For ABTS radical scavenging capacity, I. foliacea exhibited 178.5 mg VCEAC/100 mg, followed by S. nigrifolium as 80.9 mg VCEAC/100 mg. I. foliacea inhibited about 68% of superoxide radical generation followed by S. nigrifolium (40.6%) while vitamin C as positive control inhibited about 28.9% of superoxide radical generation. The antioxidant capacities measured by the three assays were positively correlated with each other. Conclusions The current study explored total antioxidant capacities of various Korean seaweeds and found I. foliacea and S. nigrifolium as the most potential antioxidant-rich food resources. Further research would be warranted to investigate bioactive compounds from S. nigrifolium and I. foliacea. Funding Sources This work was supported by the Pukyong National University Research Fund in 2018.


2020 ◽  
Vol 189 ◽  
pp. 02029
Author(s):  
Zhou Yong Dong ◽  
Hua Hua Wang ◽  
Ming Yue Li ◽  
Wei Liu ◽  
Tie Hua Zhang

Procyanidins (PC) from Vitis amurensis seeds was extracted using high-intensity pulsed electric field (PEF). The process parameters including pulse number, electric field strength, ratio of material to liquid and ethanol concentration were studied using response surface methodology, and extraction yield of the extracts were determined. Antioxidant activity of the extracts by PEF, ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and ethanol extraction (EAE) were compared based on total antioxidant capacity, DPPH radical scavenging capacity and ferrous ion chelating activity. The results revealed that the optimization of conditions of PEF are: pulse number 10; electric field strength 25 kV/cm; ratio of material to liquid 1:21 g/mL; ethanol concentration 63% (v/v), and the PC yields reached 8.23% under this condition. The PEF resulted in a highest extraction yield of PC among the four extraction methods. There was no significant difference in total antioxidant capacity of PC obtained by four methods, the DPPH radical scavenging capacity and ferrous ion chelating activity of PC extracted by PEF were close to that of UAE and MAE, which were superior to EAE. These showed that PEF was an effective method for extracting PC.


2007 ◽  
Vol 62 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Masateru Ono ◽  
Eriko Oda ◽  
Takemi Tanaka ◽  
Yoshihiko Iida ◽  
Toru Yamasaki ◽  
...  

Author(s):  
Elena Andruta MURESAN ◽  
Sevastiţa MUSTE ◽  
Simona Maria MAN ◽  
Romina Alina VLAIC ◽  
Anamaria POP ◽  
...  

The antioxidant capacity of apples peel extracts during the fruit development from three apple varieties (i.e., Ionathan, Golden Delicious and Starkrimson) were analyzed  at 7, 15, 35, 65, 107, 145 days after full bloom (DAFB). The total antioxidant capacities of extracts were evaluated using free radical scavenging effect over 1,1-diphenyl l-2-picrylhydrazyl (DPPH) radical,  being determined by a spectrophotometric method.The maximum antioxidant capacity was recorded in the apples harvested 7 DAFB.


2005 ◽  
Vol 125 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Hitoshi TOMINAGA ◽  
Yuka KOBAYASHI ◽  
Takashi GOTO ◽  
Kazuo KASEMURA ◽  
Masato NOMURA

Antioxidants ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 137 ◽  
Author(s):  
Qamar Ahmed ◽  
Alhassan Alhassan ◽  
Alfi Khatib ◽  
Syed Shah ◽  
Muhammad Hasan ◽  
...  

The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions, namely hexane, chloroform, and n-butanol were evaluated for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of A. bilimbi crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC50 (4.14 ± 0.21 μg/mL) (p < 0.05), as well as xanthine oxidase inhibitory activity with IC50 (64.84 ± 3.93 μg/mL) (p < 0.05). Afzelechin 3-O-alpha-l-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction.


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