scholarly journals The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3928
Author(s):  
Abdul Rohman ◽  
Theresia Wijayanti ◽  
Anjar Windarsih ◽  
Sugeng Riyanto
Keyword(s):  
Multivariate Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
1H Nmr ◽  
Proton Nuclear Magnetic Resonance ◽  
Orthogonal Projections ◽  
Data Set ◽  
Metabolite Fingerprinting ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography

The identification of adulteration practices of medicinal plants used as herbal medicine is very important to ensure the quality, safety, and efficacy. In this study, thin layer chromatography (TLC) and proton nuclear magnetic resonance (1H-NMR)-based metabolite fingerprinting coupled with multivariate analysis were used for authentication of Curcuma xanthorrhiza extract from Curcuma aeruginosa. Curcumin contents obtained from C. xanthorrhiza extract from various regions were in the range of 0.74%–1.23%. Meanwhile, curcumin contents obtained from C. xanthorrhiza extract adulterated with 0%, 10%, 25%, 40%, 50%, and 75% of C. aeruginosa were 1.02%, 0.96%, 0.86%, 0.69%, 0.43%, and 0.27%, respectively. The decreasing of curcumin contents in adulterant concentrations of 40% and more in C. xanthorrhiza rhizome could indicate the adulteration with other rhizomes. Multivariate analysis of PCA (principal component analysis) using data set obtained from 1H-NMR spectra clearly discriminated pure and adulterated C. xanthorrhiza with C. aeruginosa. OPLS-DA (orthogonal projections to latent structures-discriminant analysis) successfully classified pure and adulterated C. xanthorrhiza with higher R2X (0.965), R2Y (0.958), and Q2(cum) (0.93). It can be concluded that 1H-NMR-based metabolite fingerprinting coupled with PCA and OPLS-DA offers an adequate method to assess adulteration practice and to evaluate the authentication of C. xanthorrhiza extracts.

scholarly journals Deteksi Adulteran Pada Bahan Baku Sediaan Temulawak (Curcuma xanthorrhiza ROXB) Instan Secara Tlc Fingerprint Analysis

2018 ◽  
Vol 15 (1) ◽  
pp. 38
Author(s):  
Fauzan Zein Muttaqin ◽  
Nurul Aida ◽  
Aiyi Asnawi
Keyword(s):  
Principal Component Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Principal Component ◽  
Component Analysis ◽  
Fingerprint Analysis ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography

Temulawak (Curcuma xanthorrhiza Roxb) merupakan salah satu jenis tanaman unggulan yang banyak dimanfaatkan masyarakat. Pencampuran adulteran pada bahan baku sediaan temulawak dapat membahayakan kesehatan. Penelitian ini bertujuan untuk mendeteksi adulteran pada bahan baku sediaan temulawak instan. Metode yang digunakan adalah Thin Layer Chromatography (TLC) fingerprint analysis. Sidik jari KLT temulawak dibuat menggunakan rimpang temulawak yang berasal dari Cianjur, Semarang, dan Nusa Tenggara Timur. Sementara sidik jadi kunyit (Curcuma longa)sebagai adulteran utama dibuat menggunakan rimpang kunyi dari Cianjur. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 96%. Analisis kromatogram secara kemometrik menggunakan metode Principal Component Analysis (PCA). Nilai loadings Principal Component 1 (PC1) menunjukkan kurva yang linier dan data hasil scores PC1 tersebut dapat membedakan dengan baik sidik jari temulawak dari kunyit dengan nilai scores temulawak dan kunyit berada pada kuadran yang berbeda. Hasil menunjukkan bahwa nilai scores ketiga sampel temulawak instan berada di antara kuadran temulawak dan kunyit (Curcuma Longa L). Dapat disimpulkan bahwa semua sampel positif mengandung adulteran pada temulawak instan.

scholarly journals DIFFERENTIATION OF Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar BY THIN LAYER CHROMATOGRAPHY FINGERPRINT ANALYSIS

2011 ◽  
Vol 11 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Mohamad Rafi ◽  
Eti Rohaeti ◽  
Ali Miftahudin ◽  
Latifah K. Darusman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Chemical Compounds ◽  
Specific Marker ◽  
Fingerprint Analysis ◽  
Fingerprint Pattern ◽  
Curcuma Xanthorrhiza ◽  
Zingiber Cassumunar ◽  
Layer Chromatography

Turmeric (Curcuma longa), java turmeric (Curcuma xanthorrhiza) and cassumunar ginger (Zingiber cassumunar) are widely used in traditional Indonesian medicine. These three herbs have relatively similar rhizomes colour so it is difficult to be differentiated especially if they are in powder form. A rapid and reliable method, thin layer chromatography (TLC) fingerprint, has been developed in order to identify, authenticate and differentiate these three herbs through fingerprint profile of chemical compounds. TLC fingerprints of the three herbs were obtained by visualization of separate zones with visible and UV (254 and 366 nm) light. The TLC fingerprint pattern is different each other and showed a specific marker zones respectively. Therefore, TLC fingerprint can be utilized for identification, authentication and differentiation method in quality control of the three herbs tested.

scholarly journals Studies on the Isolation of Parahydroxy Benzoic Acid from the Leaves of Cassia Alata

1970 ◽  
Vol 41 (1) ◽  
pp. 89-92 ◽  
Author(s):  
MS Rahaman ◽  
AJM Moynul Hasan ◽  
MY Ali ◽  
MU Ali
Keyword(s):  
Mass Spectrometry ◽  
Benzoic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Organic Solvents ◽  
1H Nmr ◽  
Cassia Alata ◽  
Layer Chromatography

Studies were carried out on the leaves of Cassia alata. Parahydroxy benzoic acid was isolated from the leaves of Cassia alata with the help of column and thin layer chromatography using a gradient of organic solvents with increasing polarity. The compound was characterized on the basis of UV, IR, 1H-NMR and Mass spectrometry. Bangladesh J. Sci. Ind. Res. 41(1-2), 89-92, 2006

scholarly journals Synthesis and transformation in the series of 2-((5-(2,4- and 3,4-dimethoxyphenyl)-3H-1,2,4-triazole-3-yl)thio)acetic acids

2021 ◽  
Vol 14 (1) ◽  
pp. 12-16
Author(s):  
D. V. Dovbnia ◽  
A. H. Kaplaushenko ◽  
Yu. S. Frolova
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
1H Nmr ◽  
Elemental Analysis ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Physical And Chemical ◽  
Layer Chromatography ◽  
And Chemical Properties ◽  
Acetic Acids

The aim of the work is to develop preparative methods for the synthesis of 2-((5-(2,4- and 3,4-dimethoxyphenyl)-3H-1,2,4-triazole-3-yl)thio)acetic acids, to study the esterification reaction in this regard, to study physical and chemical properties of the obtained substances, and to predict their toxicity. Materials and methods. Compounds were synthesized using reagents and solvents qualified as “ch.p.”. The IUPAC nomenclature as supplemented was used during the preparation. The melting temperature was determined with the capillary method according to HFC (2.2.14) on the device PTP (M). Elemental analysis was determined with the ELEMENTAR vario EL cube analyzer (manufactured in Germany) (standard – sulfonamide). IR spectra were recorded using spectrophotometer Specord M-80 (manufactured in Germany) within the range of 4000–500 cm-1 (scanning was performed under the following conditions: slot program 3.0, time constant – τ = 3 s, scanning time 34 min, samples were analyzed in the form of tablets with potassium bromide). 1H NMR spectra were recorded using Varian VXR-300 spectrophotometer (manufactured in the USA), dimethyl sulfoxide-D6 solvent, and tetramethylsilane was used as an internal standard. The spectra were decoded using the computer program ADVASP 1.43. Thin layer chromatography was performed using Sorbfil plates (analytical, size 10 × 15 cm, base: polymer substrate, sorbent: silica gel STX-1A, grain: 5–17 μm, layer thickness: 110 m combination – silicazole). Results. The synthesis of new 2-((5-(2,4- and 3,4-dimethoxyphenyl)-3H-1,2,4-triazole-3-yl)thio)acetic acids was carried out. These products became a basis for synthesis of a number of relevant esters. Physical and chemical properties were investigated for the synthesized compounds. The structure of the obtained substances was confirmed by elemental analysis, IR-spectroscopy, 1H NMR-spectrometry, and their individuality were established by thin-layer chromatography. Computer GUSAR-online prediction of acute toxicity of 2-((5-(2,4- and 3,4-dimethoxyphenyl)-3H-1,2,4-triazole-3-yl)thio)acetic acids and their esters was performed. Conclusions. Preparative methods for the synthesis of 2-((5-(2,4- and 3,4-dimethoxyphenyl)-3H-1,2,4-triazole-3-yl)thio)acetic acids have been developed, for which esterification reactions have been studied. Thus, physical and chemical properties of the received substances were investigated, and indicators of their toxicity were predicted.

scholarly journals Synthesis, characterization and thrombolytic activity of n-benzyl piperidin 4-one phenyl hydrazone

2019 ◽  
Vol 9 (2) ◽  
pp. 180-182
Author(s):  
K. Sundaresan ◽  
V. Priyadarshini ◽  
K. Tharini
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
1H Nmr ◽  
Thrombolytic Agent ◽  
Therapeutic Use ◽  
Spectral Studies ◽  
Thrombolytic Activity ◽  
Layer Chromatography ◽  
Hydrazone Derivative

The aim of this study was to synthesize, characterization and thrombolytic activity of    N-Benzyl piperidin 4-one phenyl hydrazone derivative. Check the purity of all the synthesized compounds using thin layer chromatography. The synthesized compound was characterized by IR, 13C and 1H NMR spectral studies. The synthesized compound was subjected to thrombolytic activity. The thrombolytic activity was observed in 2 different concentrations of N-Benzyl piperidin 4-one phenyl hydrazone. Our findings support the reported therapeutic use of this compound as a thrombolytic agent in the Indian system of medicine. Keywords: N-Benzyl piperidin 4-one phenyl hydrazone, thrombolytic activity, streptokinase.

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