An ecotype collection of 98 populations of white clover (Trifolium repens L.) from pastures in the eastern USA together with five USA Ladino and five New Zealand cultivars were grown in grass swards at Raleigh, North Carolina; Palmerston North and Lincoln, New Zealand. The material was compared for leaf size, cyanogenesis, seasonal growth, % clover cover and persistence. When examined as a single group, the USA ecotypes consisted of a wide range of plant types from small-leaved acyanogenic to large-leaved cyanogenic types. This contrasted with the uniformly large-leaved acyanogenic USA Ladino cultivars which have been the principal cultivars sown in eastern USA. Various selection pressures over time together with introgression between Ladino and resident wild clover types has resulted in a wide array of plant types. At the North Carolina site, USA ecotype material generally demonstrated better growth and persistence compared to the USA Ladino and New Zealand cultivars. The best ecotype plots from the Piedmont (inland region) had 55% clover cover by the third spring compared to 12% cover from the USA cultivar, SRVR and 2% cover from the NZ cultivar, Huia. Selective pressures such as hot summers, viruses, root-feeding pests and other stresses on the local clover types have resulted in ecotype material with improved adaptive features. The USA ecotype collection is an important source of germplasm for development of improved white clovers for the eastern USA. At the New Zealand sites, the USA material demonstrated pooraverage yields compared to NZ cultivars. However, a small set of USA ecotypes showed good recovery following the dry 1995 summer at Lincoln and this material warrants closer examination to determine the adaptive mechanisms involved. As the USA ecotypes show a general lack of adaptation to New Zealand pastures, any desirable features such as heat tolerance, deeper nodal roots or virus resistance uncovered in this material will require hybridisation and backcrossing with selected elite New Zealand material to capture the benefits. Keywords: adaptation, eastern USA, ecotype populations,