Determining the effective compounds of Salvia miltiorrhiza through chromatographic fingerprinting coupled with chemometrics

2021 ◽  
Vol 9 (3) ◽  
pp. 48-58
Author(s):  
Yuan-Kai Tu ◽  
◽  
Jui-Sheng Lai ◽  
Bo-Jein Kuo ◽  
Ruo-Han Jian ◽  
...  
Keyword(s):  
High Performance ◽  
Salvia Miltiorrhiza ◽  
Herbal Medicines ◽  
Rapid Identification ◽  
Chemometric Analysis ◽  
Analysis Method ◽  
Active Components ◽  
Hplc Fingerprint ◽  
Multiple Components ◽  
Spectrum Effect

Herbs are rich in the active ingredients of drugs for preventing or treating various disorders. However, conventional bioactivity-guided separation is time and labor-intensive and neglects the additive effect of multiple components. These problems hinder the development of new medicines from natural products. This study established a chemometric analysis method that integrates processes based on the spectrum-effect relationship for the rapid identification of the primary active components of a plant. The high-performance liquid chromatography (HPLC) fingerprints of 171 Salvia miltiorrhiza extracts (SMEs) with varied constituent profiles were analyzed. Chemometric analysis was performed to establish an HPLC fingerprint–bioactivity relationship to explore the components of SMEs that contribute to the antioxidant activity and cytotoxicity effect, respectively. The results indicated that the developed strategy can be used to identify components largely contributing to particular bioactivities and re-evaluate the efficacy of previously neglected components. The present study identified not only the primary active components of S. miltiorrhiza but also the optimal ratios of constituents, validating the method for use in the future investigation and development of herbal medicines. Keywords: Chemometric, herb, antioxidant, cytotoxicity, fingerprint, Salvia miltiorrhiza.

scholarly journals Quality Control Methodology and Their Application in Analysis on HPLC Fingerprint Spectra of Herbal Medicines

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Zou Hua-Bin ◽  
Du Ai-Qin ◽  
Zhang Xin-Ling ◽  
Wei Pei-Hai ◽  
Lu Wei-Jie ◽  
...  
Keyword(s):  
Quality Control ◽  
Significant Variation ◽  
High Performance ◽  
Herbal Medicines ◽  
Principal Component ◽  
Similarity Analysis ◽  
Active Components ◽  
Hplc Fingerprint ◽  
Control Methodology

As traditional Chinese medicine (TCM) is gradually accepted by many countries, people pay much attention to the quality of herbal medicines. Because of the significant variation in active components in them, the quality control of herbal medicines is a very important issue. Nowadays, high-performance liquid chromatography (HPLC) fingerprint spectra (FPS) are widely used in identification and quality control of herbal medicines. This paper will analyze the methodology and their application in identifying and evaluating herbal medicines by means of HPLC FPS, which includes simple comparing, clustering, principal component analysis (PCA), and similarity analysis methods.

scholarly journals Improved Chromatographic Fingerprinting Combined with Multi-components Quantitative Analysis for Quality Evaluation of Penthorum chinense by UHPLC-DAD

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Wangping Deng ◽  
Tongtong Xu ◽  
Min Yang ◽  
Yajun Cui ◽  
De-an Guo
Keyword(s):  
High Performance ◽  
Herbal Medicines ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Array Detector ◽  
Chromatographic Technique ◽  
Hplc Fingerprint ◽  
Solvent Flow Rate ◽  
Principle Components Analysis ◽  
Penthorum Chinense

A high performance liquid chromatographic (HPLC) fingerprint is commonly used for quality consistency evaluation of herbal medicines. Recently, an improved chromatographic technique resulted in ultra high performance liquid chromatography (UHPLC), which could provide higher resolution in less time under higher pressure using finer particles (less than 2μm) of stationary phase. A simple and sensitive method was developed and validated for fingerprint analysis of Penthorum chinense Pursh (PC), with the simultaneous determination of seven components using UPLC coupled with a diode-array detector (DAD). It took less than 20 min for analysis of one sample. Both similarity analysis and principle components analysis (PCA) were employed to evaluate the quality consistency of 17 sample batches. The analysis was performed on a Waters ACQUITY UPLC HSS T3 (2.1 × 150 mm, 1.7 μm) column, which was maintained at 45°C and the eluents were monitored with DAD at 270 nm. A gradient elution with acetonitrile and water containing 0.075% phosphoric acid was used. The solvent flow rate was 0.4 mL/min. Standard calibration curves showed good linear behavior (R2>0.9994) in the range of 0.20-337.05 μg/mL. Acceptable repeatability (RSD<0.61%), reproducibility (RSD<2.72%), stability (RSD<1.59%) and recovery in the range of 94.7%-102.9% were obtained (precision and accuracy). The validated method was successfully applied to evaluate the quality of 21 samples of PC.

scholarly journals Screening and Characterizing Tyrosinase Inhibitors from Salvia miltiorrhiza and Carthamus tinctorius by Spectrum-Effect Relationship Analysis and Molecular Docking

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Ya-Li Wang ◽  
Guang Hu ◽  
Qian Zhang ◽  
Yu-Xiu Yang ◽  
Qiao-Qiao Li ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Amino Acid ◽  
Salvia Miltiorrhiza ◽  
Carthamus Tinctorius ◽  
Amino Acid Residues ◽  
Analysis Method ◽  
Effect Relationship ◽  
Relationship Analysis ◽  
Ms Analysis ◽  
Spectrum Effect

Tyrosinase (TYR) is a rate-limiting enzyme in the synthesis of melanin, while direct TYR inhibitors are a class of important clinical antimelanoma drugs. This study established a spectrum-effect relationship analysis method and high-performance liquid chromatography-mass spectrometry (LC-MS) analysis method to screen and identify the active ingredients that inhibited TYR in Salvia miltiorrhiza–Carthamus tinctorius (Danshen–Honghua, DH) herbal pair. Seventeen potential active compounds (peaks) in the extract of DH herbal pair were predicted, and thirteen of them were tentatively identified by LC-MS analysis. Furthermore, TYR inhibitory activities of five pure compounds obtained from the DH herbal pair were validated in the test in which kojic acid served as a positive control drug. Among them, three compounds including protocatechuic aldehyde, hydroxysafflor yellow A, and tanshinone IIA were verified to have high TYR inhibitory activity (IC50 value of 455, 498, and 1214 μM, resp.) and bind to the same amino acid residues in TYR catalytic pocket according to the results of the molecular docking test. However, the other two compounds lithospermic acid and salvianolic acid A had a weak effect on TYR, as they do not combine with the active amino acid residues or act on the active center of TYR. Therefore, the developed methods (spectrum-effect relationship analysis and molecular docking) could be used to effectively screen TYR inhibitors in complex mixtures such as natural products.

Investigation on the Characteristic Components of Dahuang Zhechong Pill Based on High-Performance Liquid Chromatography (HPLC) Fingerprint

2019 ◽  
Vol 14 (12) ◽  
pp. 1934578X1988807 ◽  
Author(s):  
Li Wu ◽  
Zi-Hui Ni ◽  
Yun-Cong Xu ◽  
Xi-Qiong Zhang ◽  
Sha-Li Du ◽  
...  
Keyword(s):  
High Performance ◽  
Principal Component ◽  
Inhibitory Effect ◽  
Hplc Method ◽  
Cytotoxicity Test ◽  
Active Components ◽  
Hplc Fingerprint ◽  
Hepatocarcinoma Cells ◽  
Aloe Emodin ◽  
Common Components

Dahuang Zhechong Pill (DHZCP) has been widely used in the treatment of hepatocarcinoma in China. The aim of our study was to identify the characteristic components of DHZCP. First, HPLC fingerprint of DHZCP was established to analyze the common components of 14 batches of DHZCP samples, which were purchased from different manufacturers. The results of HPLC fingerprint detected 164 peaks in these 14 batches of DHZCP. Through similarity analysis, cluster analysis, and principal component analysis, we identified 20 common components upon which to conduct quantitative analysis conducted by an HPLC method. After that, a cytotoxicity test was carried out to screen the active components in DHZCP. The results showed that hypoxanthine, rhein, emodin, aloe emodin, and wogonin are the active components of DHZCP for the treatment of hepatocarcinoma, as they have significant inhibitory effect against the activity of drug-resistant hepatocarcinoma cells (SMMC-7721/DOX) than others.

scholarly journals Development of HPLC Fingerprint for Quality Assessment of Bulbus Lilii

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801
Author(s):  
Kunming Qin ◽  
Hao Cai ◽  
Lijuan Zheng ◽  
Miao Zhang ◽  
Xinghai Zhang ◽  
...  
Keyword(s):  
High Performance ◽  
Quality Evaluation ◽  
Quality Analysis ◽  
Analysis Method ◽  
Hplc Fingerprint ◽  
Array Detection ◽  
Different Origins ◽  
First Time ◽  
And Storage

In this study, a simple, sensitive and reliable fingerprint analysis method by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for quality analysis of Bulbus Lilii, which is a famous traditional Chinese medicine and widely used as a restorative food. Then the developed method was subsequently applied to analyse 16 samples collected or purchased from different origins. Twelve common fingerprint peaks, including four definitely identified peaks, were found in total, and were involved for further characterisation and quality evaluation of Bulbus Lilii. The chemical quality of commercial samples collected from different origins varied obviously, indicating that more attention should be paid to the standardisation of the process of harvesting, manufacturing and storage of Bulbus Lilii. This is the first time that a new method has been established to control the quality of Bulbus Lilii through HPLC-DAD.

Development of HPLC Fingerprint Analysis of Traditional Diabetes Herbal Jamu Diabetes Plant Materials

2014 ◽  
Vol 68 (1) ◽  
Author(s):  
Gholamhossein Shams ◽  
Hassan Fahmi Ismail ◽  
Teh Liam Chee ◽  
M Abbas Ali ◽  
Fadzilah Adibah Abdul Majid ◽  
...  
Keyword(s):  
High Performance ◽  
Herbal Medicines ◽  
Fingerprint Analysis ◽  
Chromatographic Fingerprint ◽  
Plant Materials ◽  
Hplc Fingerprint ◽  
Herbal Formulation ◽  
Marker Compounds ◽  
Herbal Formulations

Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. In the present study, high-performance liquid chromatography (HPLC) detection was developed for chromatographic fingerprint analysis of the extract from grounded plant materials of Jamu Diabetes (JD), a well known herbal formulation in Malaysia. The bioactive markers rosmarinic acid, curcumin, cinnamaldehyde and andrographolide were identified from the methanol/water (80:20) extract of JD materials. The data presented in this study showed that the developed method is simple, sensitive and specific for simultaneous determination of the indicated marker compounds either qualitatively or quantitatively, and may be used as a fingerprint profile for the standardization of extractives or herbal medicines from herbal formulation JD materials.

High-Performance Thin-Layer Chromatographic Fingerprints of Triterpenoids for Distinguishing Between Isodon lophanthoides and Isodon lophanthoides var. gerardianus

2019 ◽  
Vol 102 (3) ◽  
pp. 714-719 ◽  
Author(s):  
Chaozhan Lin ◽  
Fangle Liu ◽  
Runjing Zhang ◽  
Meiting Liu ◽  
Chenchen Zhu ◽  
...  
Keyword(s):  
High Performance ◽  
Herbal Medicines ◽  
Morphological Characters ◽  
Rapid Identification ◽  
Specific Method ◽  
Chinese Herbal ◽  
Aerial Parts ◽  
Chromatographic Fingerprints ◽  
The Difference ◽  
Chemical Distribution

Abstract Background: The aerial parts of Isodon lophanthoides (Buch. Ham. ex D. Don) Hara (IL) has been officially recorded as Isodonis lophanthoidis herba by many provincial quality control standards for Chinese herbal medicines in China. Recently, it has been found that one of its varieties, I. lophanthoides var. gerardianus (Benth.) Hara (ILVG) was pretended to be I. lophanthoidis herba in herbal material markets or cultivated bases. Because of the similarity on appearance, these two close-related species were difficult to be identified by morphological characters, especially when they are dried and sliced. Objective: To establish a rapid and specific method for identification of the two herbal medicines. Method: In this paper, a fingerprint of triterpenoids by HPTLC coupled with a digital profiling was established to identify IL and distinguish it from its substitute, ILVG. The specific HPTLC fingerprints constructed by determining twelve batches of IL samples and thirteen batches of ILVG samples, intuitionally reflected the difference between the two species on HPTLC image and the peak-peak rations of chemical distribution. Results: Authentication results of nine batches of commercial samples by the above established HPTLC fingerprints exhibited coincident conclusion with that by morphological means. Conclusions: The HPTLC fingerprint is proven to be simple, repeatable, specific, and suitable for rapid identification of I. lophanthoidis herba. Highlights: An efficient method for identification and distinguishing Isodon lophanthoides from its substitute, I. lophanthoides var. gerardianus, was established. HPTLC fingerprints of ursane-type triterpenoides were constructed and validated by determining IL and ILVG samples.

scholarly journals Quality assessment of Moringa seed shells based on fingerprinting using HPLC-DAD

2020 ◽  
Vol 32 (1) ◽  
pp. 28-33
Author(s):  
Yanqin Zhu ◽  
Ping Du ◽  
Shaojun Huang ◽  
Qinhong Yin ◽  
Yaling Yang
Keyword(s):  
Quality Control ◽  
Clustering Analysis ◽  
High Performance ◽  
Principal Component ◽  
Gradient Elution ◽  
Good Precision ◽  
Analysis Method ◽  
Hplc Fingerprint ◽  
Array Detection ◽  
Comprehensive Reference

A fingerprint analysis method was established for the quality control of Moringa seed shells by high-performance liquid chromatography with diode array detection (HPLC–DAD). The HPLC–DAD separation was performed on a Thermo Hypersil Gold C18 (4.6 mm × 250 mm, 5 μm) column by gradient elution with acetonitrile–water as mobile phase. The fingerprint of Moringa seed shells was established with good precision, reproducibility, and stability obtaining within 60 min, and 13 common peaks in the fingerprint were designed. Similarity analysis, principal component analysis (PCA), and hierarchical clustering analysis (HCA) were carried out to analyze the obtained fingerprints. The similarity among 11 batches of samples in addition to No. 5 and 6 was no less than 0.92. Eleven samples could be classified into 2 clusters. The HPLC fingerprint technology and application of chemical pattern recognition can provide a more comprehensive reference for the quality control of medicinal plants.

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