Investigation on the Characteristic Components of Dahuang Zhechong Pill Based on High-Performance Liquid Chromatography (HPLC) Fingerprint

2019 ◽  
Vol 14 (12) ◽  
pp. 1934578X1988807 ◽  
Author(s):  
Li Wu ◽  
Zi-Hui Ni ◽  
Yun-Cong Xu ◽  
Xi-Qiong Zhang ◽  
Sha-Li Du ◽  
...  
Keyword(s):  
High Performance ◽  
Principal Component ◽  
Inhibitory Effect ◽  
Hplc Method ◽  
Cytotoxicity Test ◽  
Active Components ◽  
Hplc Fingerprint ◽  
Hepatocarcinoma Cells ◽  
Aloe Emodin ◽  
Common Components

Dahuang Zhechong Pill (DHZCP) has been widely used in the treatment of hepatocarcinoma in China. The aim of our study was to identify the characteristic components of DHZCP. First, HPLC fingerprint of DHZCP was established to analyze the common components of 14 batches of DHZCP samples, which were purchased from different manufacturers. The results of HPLC fingerprint detected 164 peaks in these 14 batches of DHZCP. Through similarity analysis, cluster analysis, and principal component analysis, we identified 20 common components upon which to conduct quantitative analysis conducted by an HPLC method. After that, a cytotoxicity test was carried out to screen the active components in DHZCP. The results showed that hypoxanthine, rhein, emodin, aloe emodin, and wogonin are the active components of DHZCP for the treatment of hepatocarcinoma, as they have significant inhibitory effect against the activity of drug-resistant hepatocarcinoma cells (SMMC-7721/DOX) than others.

scholarly journals Quality Control Methodology and Their Application in Analysis on HPLC Fingerprint Spectra of Herbal Medicines

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Zou Hua-Bin ◽  
Du Ai-Qin ◽  
Zhang Xin-Ling ◽  
Wei Pei-Hai ◽  
Lu Wei-Jie ◽  
...  
Keyword(s):  
Quality Control ◽  
Significant Variation ◽  
High Performance ◽  
Herbal Medicines ◽  
Principal Component ◽  
Similarity Analysis ◽  
Active Components ◽  
Hplc Fingerprint ◽  
Control Methodology

As traditional Chinese medicine (TCM) is gradually accepted by many countries, people pay much attention to the quality of herbal medicines. Because of the significant variation in active components in them, the quality control of herbal medicines is a very important issue. Nowadays, high-performance liquid chromatography (HPLC) fingerprint spectra (FPS) are widely used in identification and quality control of herbal medicines. This paper will analyze the methodology and their application in identifying and evaluating herbal medicines by means of HPLC FPS, which includes simple comparing, clustering, principal component analysis (PCA), and similarity analysis methods.

Q-marker Prediction Analysis of Rhubarb in Fengyin Decoction Based on Fingerprint and Network Pharmacology

2021 ◽  
Vol 16 (8) ◽  
pp. 1934578X2110387
Author(s):  
Ji-le Lan ◽  
Ye-ping Ruan ◽  
Zhu-jun Mao ◽  
Li-yan You ◽  
Zhong Chen
Keyword(s):  
Chinese Medicine ◽  
High Performance ◽  
Active Component ◽  
Extraction Methods ◽  
Network Pharmacology ◽  
Active Components ◽  
Quality Marker ◽  
Core Components ◽  
Aloe Emodin

Fengyin Decoction (FYD) is a traditional Chinese medicine for the treatment of epilepsy and wind paralysis. However, the potential antiepileptic active component in rhubarb (which is the most effective Chinese medicine in FYD) has not been defined. In this study, we analyzed and predicted the potential quality marker (Q-marker) of rhubarb in FYD based on fingerprint and network pharmacology. The fingerprints of FYD and rhubarb were established to analyze the transmission law of active components. Ultra-high performance liquid chromatography (UPLC) was used to study quantitatively the active components obtained by different extraction methods of FYD. Combined with network pharmacological analysis, a “components-targets-pathways” network was constructed to predict the potential Q-marker. Eight peaks were identified by FYD fingerprint: aloe-emodin, rhein, emodin, chrysophanol, physcion, cinnamaldehyde, 6-gingerol, and glycyrrhizic acid ammonium salt. The determination of the 8 active components in FYD with different extraction methods suggested that rhubarb anthraquinone may be a potential antiepileptic active component. Twelve core components, 19 targets, and 21 pathways of rhubarb were screened by network pharmacology, which further demonstrated that rhubarb played a role mainly through these components, targets, and pathways. We preliminarily predicted that compounds such as rhubarb anthraquinones were a potential Q-marker. The UPLC fingerprint and the content determination method of the 8 components established in this study were effective and feasible. The findings in this study may provide a reference for further study of quality control of FYD and lay a theoretical foundation for the study of its action mechanism. In addition, our study may provide a novel idea for the study of the Q-marker of other classical compound traditional Chinese medicines.

scholarly journals Discrimination of the Geographical Origin of the Lateral Roots of Aconitum carmichaelii Using the Fingerprint, Multicomponent Quantification, and Chemometric Methods

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4124 ◽  
Author(s):  
Lu-Lin Miao ◽  
Qin-Mei Zhou ◽  
Cheng Peng ◽  
Chun-Wang Meng ◽  
Xiao-Ya Wang ◽  
...  
Keyword(s):  
High Performance ◽  
Geographical Origin ◽  
Lateral Roots ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Similarity Analysis ◽  
Diterpenoid Alkaloids ◽  
Linear Discriminant ◽  
Hplc Fingerprint ◽  
Aconitum Carmichaelii

Fuzi is a well-known traditional Chinese medicine developed from the lateral roots of Aconitum carmichaelii Debx. It is rich in alkaloids that display a wide variety of bioactivities, and it has a strong cardiotoxicity and neurotoxicity. In order to discriminate the geographical origin and evaluate the quality of this medicine, a method based on high-performance liquid chromatography (HPLC) was developed for multicomponent quantification and chemical fingerprint analysis. The measured results of 32 batches of Fuzi from three different regions were evaluated by chemometric analysis, including similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA), and linear discriminant analysis (LDA). The content of six representative alkaloids of Fuzi (benzoylmesaconine, benzoylhypaconine, benzoylaconine, mesaconitine, hypaconitine, and aconitine) were varied by geographical origin, and the content ratios of the benzoylmesaconine/mesaconitine and diester-type/monoester-type diterpenoid alkaloids may be potential traits for classifying the geographical origin of the medicine. In the HPLC fingerprint similarity analysis, the Fuzi from Jiangyou, Sichuan, was distinguished from the Fuzi from Butuo, Sichuan, and the Fuzi from Yunnan. Based on the HCA and PCA analyses of the content of the six representative alkaloids, all of the batches were classified into two categories, which were closely related to the plants’ geographical origins. The Fuzi samples from Jiangyou were placed into one category, while the Fuzi samples from Butuo and Yunnan were put into another category. The LDA analysis provided an efficient and satisfactory prediction model for differentiating the Fuzi samples from the above-mentioned three geographical origins. Thus, the content of the six representative alkaloids and the fingerprint similarity values were useful markers for differentiating the geographical origin of the Fuzi samples.

scholarly journals Simultaneous Determination of 6 Antiallergic Components in Asarum sieboldii Using High-Performance Liquid Chromatography

2020 ◽  
Vol 15 (10) ◽  
pp. 1934578X2096619
Author(s):  
Seol Jang ◽  
Sun Haeng Park ◽  
Ho Kyoung Kim
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Inhibitory Effect ◽  
Hplc Method ◽  
Inhibitory Effects ◽  
Traditional Herbal Medicine ◽  
Simultaneous Quantification ◽  
Antiallergic Effect

Owing to the side effects of current drugs for treating atopic dermatitis (AD), a chronic disease in the skin, traditional herbal medicine is receiving much attention as an alternative treatment. Asarum sieboldii has traditionally been used to treat colds, fevers, headaches, coughs, neuralgia, chronic bronchitis, asthma, and allergies. In this study, 6 compounds (echinacoside, vanillic acid, kakuol, methyl eugenol, sesamin, and asarinin) in A. sieboldii were analyzed simultaneously using high-performance liquid chromatography (HPLC), and the proposed analytical method was validated. In addition, the inhibitory effects of the A. sieboldii extract and the 6 analyzed compounds on the expression of chemokine were evaluated in HaCaT cells. The HPLC method showed high linearity, with a correlation coefficient of ≥0.9999. The limits of detection for the 6 compounds ranged from 0.00 to 0.02 µg/mL, and the limits of quantification ranged from 0.01 to 0.05 µg/mL. The intraday and interday precisions were 0.15%-1.90%; the accuracy was 97.36%-103.23%, and the recoveries of the 6 compounds were 97.45%-103.93%. The content of each compound in A. sieboldii, as determined using the corresponding calibration curve, was in the range of 0.380-12.062 mg/g. This optimized simultaneous quantification method will be suitable for improving the quality control of A. sieboldii. Moreover, the 6 compounds in A. sieboldii showed an inhibitory effect on the production of chemokines, which suggests that A. sieboldii has an antiallergic effect.

scholarly journals Determination and Chemometrics-Assisted Comparative Analysis of Active Components in Different Tissue of Rana chensinensis

Separations ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 164
Author(s):  
Jianqiu Zhang ◽  
Zhongyao Wang ◽  
Shihan Wang ◽  
Changli Zhang ◽  
Nan Li ◽  
...  
Keyword(s):  
High Performance ◽  
Rana Temporaria ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Comparative Approach ◽  
Active Components ◽  
Rana Chensinensis ◽  
Development And Utilization ◽  
Further Development ◽  
Different Tissues

In this study, the chemical composition of different tissues of Rana temporaria chensinensis David derived from the same individual was analyzed by comparative approach. First, pre-column derivatization combined with high performance liquid chromatography (HPLC) was established to determine the content of 1-methyl hydantoin in samples, which used S1–S5 samples. The results indicated that 1-methyl hydantoin was determined in Oviductus Ranae (OR), Rana chensinensis ovum (RCO), Rana chensinensis meat (RCM), and Rana chensinensis skin (RCS), except for Rana chensinensis bone (RCB). Moreover, the content of it in RCS was the highest. In addition, the contents of six polyunsaturated fatty acids (PUFAs) in different tissues of Rana chensinensis were measured by HPLC, including eicosapentaenoic acid (EPA), α-linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA). The results indicated that OR, RCO, RCM, RCS, and RCB all contained the above six PUFAs. With the aid of chemometrics methods, the results of principal component analysis (PCA), hierarchical cluster analysis (HCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) combined with the sequencing results of the total PUFAs content of each sample, showed that different tissues of Rana chensinensis could be divided into four categories, and the RCO sample was divided into one category because of the highest PUFAs content, which was a good source of PUFA. For comparison, OR and other tissue from the perspective of PUFAs, we also established OPLS-DA models of them. It could be found that the RCM was the most similar to the OR in the diversity and content of PUFAs. This study provided a theoretical basis for the further development and utilization of RCO, RCM, RCS, and RCB as by-products of OR.

scholarly journals A Strategy for Quality Control of Vespa magnifica (Smith) Venom Based on HPLC Fingerprint Analysis and Multi-Component Separation Combined with Quantitative Analysis

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2920 ◽  
Author(s):  
Si-Tong Zhou ◽  
Kai Luan ◽  
Lian-Li Ni ◽  
Ying Wang ◽  
Shi-Meng Yuan ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
High Performance ◽  
Principal Component ◽  
Good Precision ◽  
Fingerprint Analysis ◽  
Wasp Venom ◽  
Chemical Fingerprint ◽  
Hplc Fingerprint ◽  
Relative Standard

As a folk medicine of the Jingpo minority in Yunnan province, the venom of Vespa magnifica has been commonly used for the treatment of rheumatoid arthritis. Quality standardization of the wasp venom is a necessary step for its pharmaceutical research and development. To control the quality of the wasp venom, a method based on high-performance liquid chromatography (HPLC) was developed for chemical fingerprint analysis. In the chromatographic fingerprinting, chemometrics procedures, including similarity analysis (SA), hierarchical clustering analysis (HCA), and principal component analysis (PCA), were applied to classify 134 batches (S1–S134) of wasp venom from different origins. The HPLC fingerprint method displayed good precision (Relative standard deviation, RSD < 0.27%), stability (in 16 h, RSD < 0.34%), and repeatability (RSD < 1.00%). Simultaneously, four compounds (VMS1, VMS2, VMS3, and VMS4) in the wasp venom were purified and identified. VMS1 was 5-hydroxytryptamine, and the other compounds were three peptides that were sequenced as follows: Gly–Arg–Pro–Hyp–Gly–Phe–Ser–Pro–Phe–Arg–Ile–Asp–NH2 (VMS2), Ile–Asn–Leu–Lys–Ala–Ile–Ala–Ala–Leu–Ala–Lys–Lys–Leu–Leu–NH2 (VMS3), and Phe–Leu–Pro–Ile–Ile–Gly–Lys–Leu–Leu–Ser–Gly–Leu–Leu–NH2 (VMS4). The quantifications for these components were 110.2 mg/g, 26.9 mg/g, 216.3 mg/g, and 58.0 mg/g, respectively. The results of this work indicated that the combination of the chemical fingerprint and quantitative analysis offers a reasonable way to evaluate the quality of wasp venom.

scholarly journals Development and Validation of a Stability-Indicating HPLC Method for the Quantification of α-Mangostin in Dietary Supplements

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986394 ◽  
Author(s):  
Blanca Rivero ◽  
Iliana Garibay
Keyword(s):  
Dietary Supplements ◽  
High Performance ◽  
Degradation Products ◽  
Principal Component ◽  
Hplc Method ◽  
Garcinia Mangostana ◽  
Reverse Phase ◽  
Pharmacological Activities ◽  
Stability Indicating ◽  
Development And Validation

Xanthones are the main active constituents isolated from Garcinia mangostana L. (Hypericaceae). Among these, α-mangostin (1) is reported as the principal component of the species, with remarkable pharmacological activities. In this work, a reverse-phase high-performance liquid chromatography (HPLC) method was developed as a suitable stability-indicating procedure for the quantification of 1 in the presence of degradation products. Chromatographic analysis was performed on a Purospher STAR C18 column with a mobile phase containing 0.1% trifluoroacetic acid in water and ACN (20:80) at a flow rate of 1.0 mL/min. The method was linear over the concentration range of 10 to 100 μg/mL. The limits of detection and quantification were 0.13 and 0.4 μg/mL, respectively. Stability studies were carried out at temperature/relative humidity of 30°C/60% and 40°C/75% during 6 months; and no significant changes were observed. According to the global results, a simple HPLC method was developed and fully validated for quantifying 1 in mangosteen dietary supplements.

scholarly journals HPLC Fingerprint Combined With Multicomponent Quantification as an Efficient Method for Quality Evaluation of Pharbitidis Semen

2020 ◽  
Vol 15 (6) ◽  
pp. 1934578X2093164
Author(s):  
Huiwei Bao ◽  
Huailei Yang ◽  
Feng Wang ◽  
Kaixuan Zhou ◽  
Yanan Yang ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Caffeic Acid ◽  
Evaluation System ◽  
High Performance ◽  
Quality Evaluation ◽  
Hplc Method ◽  
Hplc Fingerprint ◽  
Protocatechuic Aldehyde ◽  
Effective Components

Pharbitidis Semen is a traditional Chinese medicine(TCM) with a long history for treatment of edema and fullness, fecal and urinary retention, phlegm and retained fluid, and abdominal pain due to parasitic infestation. Since Pharbitidis Semen is distributed throughout the country, the quality of the medicine from different origins may be varied. Moreover, the reported method could not control the quality comprehensively. In this article, a fingerprint of Pharbitidis Semen has been established based on a high-performance liquid chromatography (HPLC) method. In addition, the contents of the 2 main effective components were determined simultaneously. The reference HPLC fingerprint was obtained according to the chromatograms of test samples. The similarity values were calculated by the Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 A edition). Cluster analysis of 10 batches of samples was performed using statistical software (SPSS 20.0). The HPLC fingerprints of 10 batches of Pharbitidis Semen showed 25 well-resolved common peaks in each chromatogram. Two of these peaks were assigned to protocatechuic aldehyde and caffeic acid. As a result, HPLC fingerprint similarities of 10 batches of samples were more than 0.99. Pharbitidis Semen from different habitats could be divided into 3 or 2 groups. The results of cluster analysis showed that samples classified into 1 group were associated with their habitats and breeds. At the same time, quantification results showed that the contents of protocatechuic aldehyde and caffeic acid were in the range of 0.026-0.088 and 0.019-0.053 mg/g−1 respectively. HPLC fingerprint combined with multicomponent quantification and data analysis techniques can be an efficient and useful method for monitoring the quality of Pharbitidis Semen. This study also provides a practical strategy for overall quality evaluation and control of traditional Chinese medicines.

Determining the effective compounds of Salvia miltiorrhiza through chromatographic fingerprinting coupled with chemometrics

2021 ◽  
Vol 9 (3) ◽  
pp. 48-58
Author(s):  
Yuan-Kai Tu ◽  
◽  
Jui-Sheng Lai ◽  
Bo-Jein Kuo ◽  
Ruo-Han Jian ◽  
...  
Keyword(s):  
High Performance ◽  
Salvia Miltiorrhiza ◽  
Herbal Medicines ◽  
Rapid Identification ◽  
Chemometric Analysis ◽  
Analysis Method ◽  
Active Components ◽  
Hplc Fingerprint ◽  
Multiple Components ◽  
Spectrum Effect

Herbs are rich in the active ingredients of drugs for preventing or treating various disorders. However, conventional bioactivity-guided separation is time and labor-intensive and neglects the additive effect of multiple components. These problems hinder the development of new medicines from natural products. This study established a chemometric analysis method that integrates processes based on the spectrum-effect relationship for the rapid identification of the primary active components of a plant. The high-performance liquid chromatography (HPLC) fingerprints of 171 Salvia miltiorrhiza extracts (SMEs) with varied constituent profiles were analyzed. Chemometric analysis was performed to establish an HPLC fingerprint–bioactivity relationship to explore the components of SMEs that contribute to the antioxidant activity and cytotoxicity effect, respectively. The results indicated that the developed strategy can be used to identify components largely contributing to particular bioactivities and re-evaluate the efficacy of previously neglected components. The present study identified not only the primary active components of S. miltiorrhiza but also the optimal ratios of constituents, validating the method for use in the future investigation and development of herbal medicines. Keywords: Chemometric, herb, antioxidant, cytotoxicity, fingerprint, Salvia miltiorrhiza.

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