Screening of Vietnamese soybean genotypes for Agrobacterium-mediated transgenic transformation

Soybean [Glycine max (L) Merr.] is one of the most important crops used for human food and animal feed globally. Transgenic soybean covers more than 74% of the global soybean production area, which is an achievement of genetically modified programs. The Agrobacterium-mediated method is commonly used for soybean transformation, but the efficiency of this method is affected by various factors including genotypes. Screening of the soybean genotypes suitable for Agrobacterium-infection and plant regeneration is the most important step to establish an efficient genetic transformation system. In this study, we screened thirty Vietnamese soybean genotypes including seventeen cultivated soybean genotypes (CSG) and thirteen local soybean genotypes (LCG) for shoot regeneration ability and transient infection via Agrobacterium tumefaciens method. Two CSG cultivars, DT22 and VX93, had significantly high efficiencies for shoot regeneration and transient infection compared with the control genotypes Jack and William 82. The shoot regeneration of DT22 and VX93 was 92.32% with 5.75 shoots/explant and 93.35% with 5.92 shoots/explant, respectively, whereas the control genotypes Jack and William 82 had 91.35% with 4.6 shoots/explant and 82.64% with 5.7 shoots/explant. Similarly, the transient infection of DT22 and VX93 was 84% and 86%, respectively, which was comparable with that of Jack (86%) William (82%). The success of transgenic development was confirmed by the β-Glucuronidase staining, PCR, and Basta leaf painting. The results indicated that cultivars DT22 and VX93 could be used for stable Agrobacterium-media transformation. Keywords: Soybean, Agrobacterium, transformation, transient infection, transgenic.

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Genotypic and environmental variation in seed nutraceutical and industrial composition of non-transgenic soybean (Glycine max) genotypes

Crop and Pasture Science ◽

10.1071/cp14114 ◽

2014 ◽

Vol 65 (12) ◽

pp. 1311 ◽

Cited By ~ 9

Author(s):

Constanza S. Carrera ◽

Julio L. Dardanelli ◽

Diego O. Soldini

Keyword(s):

Glycine Max ◽

Environmental Conditions ◽

Raw Material ◽

Superior Performance ◽

Chemical Components ◽

Transgenic Soybean ◽

Wide Range ◽

Soybean Genotypes ◽

Glycine Max L ◽

Design Production

Genotype × environment interactions (G × E) induce differential response of soybean (Glycine max (L.) Merr.) genotypes to variable environmental conditions with respect to seed composition, and this may hinder breeding progress. The objectives of this study were to estimate the contribution of genotype, environment and G × E to seed chemical composition variability, and to identify the most stable non-transgenic genotypes for several chemical components. Seeds from six non-transgenic soybean genotypes that were grown in 23 environments in Argentina (24–38°S) were analysed. Although environment was the most important source affecting variation for most of the analysed chemical components, genotype and G × E also had a significant effect (P < 0.001). Stable genotypes with superior performance across a wide range of environments were ALIM3.20 for protein, linolenic acid (Len), Len : linoleic acid (LA) ratio (Len/LA), δ-tocopherol (δT) and total isoflavones (TI); ALIM4.13 for protein, oleic acid, α-tocopherol (αT) and δT; ALIM3.14 for Len, αT and TI; Ac0124-1 for Len and Len/LA; and Ac0730-3 for αT. Non-transgenic genotypes with stable chemical profile across environments would perform well under a wide range of environmental conditions for any chemical compound. This study contributes knowledge for breeders to use these genotypes to broaden the genetic backgrounds of currently available commercial cultivars, or to design production strategies that employ the genotypes directly as raw material.

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Identification of novel haplotype of a cyst nematode resistance gene, GmSNAP18 in soybean [Glycine max (L.) Merr.]

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.80.3.5 ◽

2020 ◽

Vol 80 (03) ◽

Author(s):

Ik-Young Choi ◽

Prakash Basnet ◽

Hana Yoo ◽

Neha Samir Roy ◽

Rahul Vasudeo Ramekar ◽

...

Keyword(s):

Soybean Cyst Nematode ◽

Gene Families ◽

Nematode Resistance ◽

Cyst Nematode ◽

Soybean Breeding ◽

Resistant Varieties ◽

Soybean Genotypes ◽

Glycine Max L ◽

Scn Resistance

Soybean cyst nematode (SCN) is one of the most damaging pest of soybean. Discovery and characterization of the genes involved in SCN resistance are important in soybean breeding. Soluble NSF attachment protein (SNAP) genes are related to SCN resistance in soybean. SNAP genes include five gene families, and 2 haplotypes of exons 6 and 9 of SNAP18 are considered resistant to the SCN. In present study the haplotypes of GmSNAP18 were surveyed and chacterized in a total of 60 diverse soybean genotypes including Korean cultivars, landraces, and wild-types. The target region of exons 6 and 9 in GmSNAP18 region was amplified and sequenced to examine nucleotide variation. Characterization of 5 haplotypes identified in present study for the GmSNAP18 gene revealed two haplotypes as resistant, 1 as susceptible and two as novel. A total of twelve genotypes showed resistant haplotypes, and 45 cultivars were found susceptible. Interestingly, the two novel haplotypes were present in 3 soybean lines. The information provided here about the haplotypic variation of GmSNAP18 gene can be further explored for soybean breeding to develop resistant varieties.

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Economic optimum plant density of irrigated early-maturity soybean in southern Alberta

Canadian Journal of Plant Science ◽

10.1139/cjps-2021-0026 ◽

2021 ◽

Author(s):

Tram T.N. Thai ◽

Danny G. Le Roy ◽

Manjula S. Bandara ◽

James E. Thomas ◽

Francis J Larney

Keyword(s):

Plant Density ◽

Profit Maximization ◽

Seeding Density ◽

Early Maturity ◽

Growing Seasons ◽

Southern Alberta ◽

Soybean Genotypes ◽

Glycine Max L ◽

The Difference ◽

Lower Plant

With soybean [Glycine max (L.) Merr.] seed cost increasing in Alberta, understanding economic optimum plant density (EOPD) could help growers save on input expenses. A study was conducted at two irrigated locations in southern Alberta (Bow Island and Lethbridge), in three growing seasons (2014–16), using two maturity group (MG) 00 soybean genotypes, two row spacings (RS; narrow, 17.5 cm; wide, 35 cm), and three seeding densities (SD; 30, 50 and 80 seeds m-2). Exponential plant density-yield relationships were used to estimate EOPD. The earlier MG 00.4 genotype compensated yield at lower plant density (39 vs. 43 plants m-2) and emergence (74 vs. 80%) than the later MG 00.8 genotype. The EOPD gaps between environments, genotypes, and RS were minimal (from 1–3 plants m-2), resulting in only 1.3–2.0% differences in grain yield (37–56 kg ha-1), and gross revenue at EOPD ($16–24 ha-1). The overall EOPD estimate was 46 plants m-2, regardless of environment, genotype or RS. The study highlighted the difference between agronomic production and profit maximization in choosing an optimum plant density, and the need to establish a seeding density calculator for irrigated soybean in southern Alberta.

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Assessment of Yield and Yield Component of Soybean Genotypes (Glycine Max L.) in North of Khuzestan

Journal of Crop Science and Biotechnology ◽

10.1007/s12892-018-0023-0 ◽

2018 ◽

Vol 21 (5) ◽

pp. 435-441 ◽

Cited By ~ 1

Author(s):

Sina Ghanbari ◽

Ahmad Nooshkam ◽

Barat Ali Fakheri ◽

Nafiseh Mahdinezhad

Keyword(s):

Glycine Max ◽

Yield Component ◽

Soybean Genotypes ◽

Glycine Max L

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MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds

International Journal of Molecular Sciences ◽

10.3390/ijms222112017 ◽

2021 ◽

Vol 22 (21) ◽

pp. 12017

Author(s):

Ming-Sin Ng ◽

Yee-Shan Ku ◽

Wai-Shing Yung ◽

Sau-Shan Cheng ◽

Chun-Kuen Man ◽

...

Keyword(s):

Animal Feed ◽

Cell Model ◽

Vacuolar Membrane ◽

Food Crops ◽

Soybean Seeds ◽

Transgenic Soybean ◽

Bona Fide ◽

Isoflavone Biosynthesis ◽

Seed Isoflavone ◽

Total Seed

Soybeans are nutritionally important as human food and animal feed. Apart from the macronutrients such as proteins and oils, soybeans are also high in health-beneficial secondary metabolites and are uniquely enriched in isoflavones among food crops. Isoflavone biosynthesis has been relatively well characterized, but the mechanism of their transportation in soybean cells is largely unknown. Using the yeast model, we showed that GmMATE1 and GmMATE2 promoted the accumulation of isoflavones, mainly in the aglycone forms. Using the tobacco BrightYellow-2 (BY-2) cell model, GmMATE1 and GmMATE2 were found to be localized in the vacuolar membrane. Such subcellular localization supports the notion that GmMATE1 and GmMATE2 function by compartmentalizing isoflavones in the vacuole. Expression analyses showed that GmMATE1 was mainly expressed in the developing soybean pod. Soybean mutants defective in GmMATE1 had significantly reduced total seed isoflavone contents, whereas the overexpression of GmMATE1 in transgenic soybean promoted the accumulation of seed isoflavones. Our results showed that GmMATE1, and possibly also GmMATE2, are bona fide isoflavone transporters that promote the accumulation of isoflavones in soybean seeds.

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Proteomic and genomic characterization of Kunitz trypsin inhibitors in wild and cultivated soybean genotypes

Journal of Plant Physiology ◽

10.1016/j.jplph.2006.05.014 ◽

2007 ◽

Vol 164 (6) ◽

pp. 756-763 ◽

Cited By ~ 22

Author(s):

Savithiry Natarajan ◽

Chenping Xu ◽

Hanhong Bae ◽

Bryan A. Bailey

Keyword(s):

Trypsin Inhibitors ◽

Soybean Genotypes ◽

Genomic Characterization ◽

Cultivated Soybean

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Dissimilarity in low molecular weight carbohydrate composition of the seeds of cultivated soybean (Glycine max (L.) MERRILL subsp. max) and wild soybean(G. max subsp. soja (SIEB. et ZUCC.) OHASHI).

Agricultural and Biological Chemistry ◽

10.1271/bbb1961.49.933 ◽

1985 ◽

Vol 49 (4) ◽

pp. 933-937 ◽

Cited By ~ 3

Author(s):

Takeshi YASUI

Keyword(s):

Molecular Weight ◽

Glycine Max ◽

Wild Soybean ◽

Low Molecular Weight ◽

Carbohydrate Composition ◽

Glycine Max L ◽

Cultivated Soybean

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Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes

Czech Journal of Genetics and Plant Breeding ◽

10.17221/6114-cjgpb ◽

2012 ◽

Vol 38 (No. 2) ◽

pp. 69-74 ◽

Cited By ~ 2

Author(s):

M. Baránek ◽

M. Kadlec ◽

J. Raddová ◽

M. Vachůn ◽

M. Pidra

Keyword(s):

Genetic Diversity ◽

Glycine Max ◽

Random Amplified Polymorphic Dna ◽

Breeding Programs ◽

Low Level ◽

Low Degree ◽

Soybean Genotypes ◽

Glycine Max L ◽

Rapd Polymorphism ◽

Selection Of

The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19 soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD polymorphism in the case of Glycine max, fragments with low level of informativeness were evaluated, too. Presented results enable the selection of genetically distinct individuals. Such information may be useful to breeders willing to use genetically diverse introductions in soybean improvement process. 

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