Study of the diagnostic significance of telomere length and telomerase activity in patients with cerebral atherosclerosis and type 2 diabetes mellitus

Aim.To study the association of chronic inflammation, oxidative stress with telomere biology in people with type 2 diabetes mellitus (T2DM).Material and Methods.A total 50 patients with T2D and without cardiovascular disease (CVD) and 139 people from control group were included in the study. All subjects were measured for carbohydrate metabolism; oxidative stress (malondialdehyde (MDA)); inflammation (C-reactive protein — CRP, fibrinogen, interleukin-6); lymphocyte telomere length, telomerase activity.Results.In diabetic patients telomeres were shorter than in controls (9.59±0.54 and 9.76±0.47; p=0.031), telomerase activity was lower (0.47±0.40 and 0.62±0.36; p=0.039), inflammation (CRP, elevated fibrinogen) was higher. All patients were divided by telomere length. In T2DM group CRP was higher in patients with «short» telomeres (7.39±1.47 and 3.59±0.58 mg/L; p=0.02). There were no significant differences in the level of chronic inflammation and oxidative stress in ‘long’ telomeres group: CRP 3.59±0.58 and 3.66±0.50 mg/L (p=0.93), MDA 2.81±0.78 and 3.24±0.78 mmol/l (p=0.08). Diabetic patients in «short» telomeres group had greater chronic inflammation: CRP 7.39±1.47 and 4.03±0.62 mg/L (p=0.046), increased fibrinogen, 0.371 and 0.159 (p=0.022). All patients were divided by telomerase activity. Severity of chronic inflammation was greatest in T2DM and the «low» activity of telomerase. There were relationship between telomere length and CRP in T2DM patients (r=–0.40; p=0.004).Conclusions. Chronic inflammation and cell aging were more pronounced in patients with T2DM. However, despite diabetes, signs of chronic inflammation were minimal in patients with «long» telomeres compared to healthy people. Perhaps long telomeres protect diabetic patients from the damaging effect of chronic inflammation.

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The Changes of Leukocyte Telomere Length after Different Treatments in Patients with Type 2 Diabetes Mellitus (T2DM)

Diabetes ◽

10.2337/db18-2356-pub ◽

2018 ◽

Vol 67 (Supplement 1) ◽

pp. 2356-PUB

Author(s):

JUANHONG LIU ◽

DELIN MA ◽

YAN YANG

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Telomere Length ◽

Leukocyte Telomere Length

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Telomere biology and metabolic disorders: the role of insulin resistance and type 2 diabetes

Problems of Endocrinology ◽

10.14341/probl12510 ◽

2020 ◽

Vol 66 (4) ◽

pp. 35-44

Author(s):

Ekaterina N. Dudinskaya ◽

Olga N. Tkacheva ◽

Natalia V. Brailova ◽

Irina D. Strazhesko ◽

Marina V. Shestakova

Keyword(s):

Diabetes Mellitus ◽

Insulin Resistance ◽

Type 2 Diabetes ◽

Carbohydrate Metabolism ◽

Telomere Length ◽

Telomerase Activity ◽

Cellular Aging ◽

The Mean ◽

Telomere Biology

BACKGROUND: Insulin resistance accelerates the aging process, but its speed depends on the individual characteristics of the metabolism. One of the reasons for the different aging rates in individuals with insulin resistance is the initially different “genetic protection” of cells, which many scientists associate with replicative cellular aging.AIMS: to study the relationship between the state of carbohydrate metabolism and markers of replicative cell aging in individuals with different sensitivity to insulin.MATERIALS AND METHODS: The observation study included 305 patients. The parameters of glucose metabolism and telomere biology were studied.RESULTS: The mean age of the patients was 51.5±13.3 years. Patients were divided into three groups depending on presence of insulin resistance: healthy, with insulin resistance and with type 2 diabetes. The mean age of healthy patients was 48.82±13.87 years, in insulin resistance group — 53.04±12.8, in 2 diabetes mellitus — 58.4±7.90. The median telomere length was 9.76. The median telomerase activity was 0.48. Both telomere length and telomerase activity progressively decrease as insulin resistance increases. In patients with diabetes, short telomere lengths and low telomerase activity predominated. The insulin resistance index has the greatest impact on the risk of detecting “short” telomeres. In patients with insulin resistance, an increase in glycated hemoglobin increases the likelihood of detecting short telomeres by 2.4 times, and in diabetes mellitus by 4.26 times, an increase in fasting plasma glucose by 90%, and an increase in HOMA-IR by 35%. An increase in insulin resistance increases the risk of detecting «low» telomerase activity by 53% and the risk of detecting «very low» telomerase activity by 92%. A decrease in synsulin resistance increases the chance of increasing telomerase activity to «very high» by 51%.CONCLUSION: Shorter telomeres are associated with more pronounced disorders of carbohydrate metabolism and a higher degree of insulin resistance. Further studies of metabolic status are necessary to personalize their lifestyle and treatment goals.

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The shortening of leukocyte telomere length relates to DNA hypermethylation of LINE-1 in type 2 diabetes mellitus

Oncotarget ◽

10.18632/oncotarget.18167 ◽

2017 ◽

Vol 8 (43) ◽

pp. 73964-73973 ◽

Cited By ~ 9

Author(s):

Yue Wu ◽

Wei Cui ◽

Donghong Zhang ◽

Wei Wu ◽

Zhuo Yang

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Telomere Length ◽

Leukocyte Telomere Length ◽

Dna Hypermethylation

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TELOMERES LENGTH IN PATIENTS WITH TYPE 2 DIABETES MELLITUS AND ITS RELATION TO METABOLIC PROFILE

Fiziolohichnyĭ zhurnal ◽

10.15407/fz66.06.049 ◽

2020 ◽

Vol 66 (6) ◽

pp. 49-55

Author(s):

N.V. Kharchenko ◽

◽

M.S. Romanenko ◽

L.L. Sineok ◽

D.S. Krasnienkov ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Waist Circumference ◽

Telomere Length ◽

Metabolic Profile ◽

Leukocyte Telomere Length ◽

C Reactive Protein ◽

Reactive Protein

To study leukocyte telomere length and its relationship with metabolic profile 35 patients with metabolic syndrome and type 2 diabetes mellitus (T2DM) and 21 healthy people of middle age (35-59 years) were examined. The anthropometric characteristics of obesity, indicators of lipid and glucose metabolism, alanin aminotransferase (ALT) and high sensitive C-reactive protein levels were studied. The relative average telomere length was determined by the method of monochrome multiplex quantitative real time polymerase chain reaction. Patients with T2DM had higher BMI, waist circumference, higher high sensitive C-reactive protein, ALT and glucose levels and a worse lipid profile (p <0.05). At the same time, the median telomere length did not differ between groups. Nevertheless, in the T2DM group the telomere length inversely correlated with body weight (r = –0.35; p < 0.05), BMI (r = –0.36; p < 0.05), waist circumference (r = –0.34; p < 0.05) and ALT level (r = –0,44; p<0,05) in contrast to healthy subjects. No relationship was found between the telomere length and the level of fasting glycemia, as well as the age of the participants of both groups. Thus, in T2DM patients increase in BMI, waist circumference and ALT level were associated with a shorter leukocyte telomere length. Despite the worse metabolic profile, the telomere length in middle-aged T2DM patients did not differ from that in the control group. This indicates that the leukocyte telomere length is influenced not only by the presence of T2DM and the metabolic profile indicators, but, obviously, by other factors as well.

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Association of leukocyte telomere length with metabolic syndrome in type 2 diabetes mellitus

Journal of Research in Medical Sciences ◽

10.4103/jrms.jrms_793_20 ◽

2021 ◽

Vol 26 (1) ◽

pp. 43

Author(s):

Kun Dong ◽

Xuemin Peng ◽

Jiaojiao Huang ◽

Sanshan Xia ◽

Yan Yang

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Metabolic Syndrome ◽

Type 2 Diabetes Mellitus ◽

Telomere Length ◽

Leukocyte Telomere Length

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Abstract 12022: Telomere Length, Telomerase Activity and Vascular Aging in Patients With Type 2 Diabetes Mellitus

Circulation ◽

10.1161/circ.132.suppl_3.12022 ◽

2015 ◽

Vol 132 (suppl_3) ◽

Author(s):

Natalia Brailova ◽

Ekaterina Dudinskaya ◽

Valentina Pykhtina ◽

Ekaterina Plochova ◽

Irina Strazhesko ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Telomerase Activity ◽

Cellular Aging ◽

Healthy People ◽

Diabetic Patients ◽

Vascular Aging

Introduction: Type 2 diabetes mellitus (T2DM) contribute to vascular aging. The telomere length (TL) and telomerase activity (TA) are considered as biomarkers of cellular aging. TL and TA are insufficiently studied in diabetic patients. Hypothesis: Telomere biology is associated with vascular aging in diabetic patients. Methods: The study group included 50 patients with T2DM (mean age 58.4±7.83 years) and 139 healthy patients (mean age 57.45±8.14 years). All subjects were measured for TL and TA by quantitative polymerase chain reaction; oxidative stress marked by malondialdehyde (MDA); inflammation marked by C-reactive protein (CRP); arterial stiffness evaluated by pulse wave velocity (PWV); carotid intima-media thickness (IMT), plaque presence (PP) and endothelial dysfunction evaluated by flow-mediated endothelium-dependent vasodilation (FMV). Results: All patients were divided into 4 groups by the median of TL (9.75): «short» telomeres (T2DM+ (n=15) and T2DM- (n=63) and «long» telomeres (T2DM+ (n=35) and T2DM- (n=76)). Patients with T2DM and «long» TL had the state of vessels, oxidative stress, inflammation, TA as similar as in healthy people: PWV 11.54±3.57 (T2DM+) vs 10.98±1.83 m/s (T2DM-) ((=0.58), IMT 0.83±0.13 vs 0.76±0.16 mm (=0.13), PP 1.36±0.33 vs 1.23±0.20 (=0.79); MDA 2.81±0.78 vs 3.24±0.78 mkmol/l (=0.08); CRP 3.59±0.58 vs 3.66±0.50 mg/l (=0.93); TA 0.51±0.09 vs 0.60±0.05 (p=0.36). FMV was higher in diabetic patients: 11.87±3.36 vs 10.18±2.79 % (=0.049). In contrast patients with «short» TL and T2DM had more pronounced vascular aging, inflammation and lower TA than healthy people: PWV 13.48±3.24 vs 11.59±2.03 m/s (=0.003), IMT 0.95±0.17 vs 0.78±0.14 mm (<0.001), PP 2.23±0.27 vs 1.38±0.17 (=0.006), FMV 8.51±3.20 vs 11.04±3.01% (=0.0002; CRP 7.39±1.47 vs 4.03±0.62 mg/l (=0.046); TA 0.47±0.08 vs 0.62±0.07 (p=0.06). Conclusion: In patients with short telomeres and T2DM signs of vascular aging, chronic inflammation and cellular aging were more pronounced than in healthy people. In contrast, in patients with long telomeres and T2DM vascular changes, oxidative stress, chronic inflammation and TA were as similar as in healthy people. Perhaps long telomeres protect patients with T2DM from accelerated vascular aging.

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