scholarly journals Identification of the causative agent of canine babesiosis in the Kirov Region

2021 ◽  
Vol 15 (2) ◽  
pp. 24-28
Author(s):  
O. O. Skornyakova
Keyword(s):  
Image Analysis ◽  
Causative Agent ◽  
Mixed Infection ◽  
Parasite Species ◽  
Digital Camera ◽  
Acute Angle ◽  
Canine Babesiosis ◽  
City Environment ◽  
Binocular Microscope ◽  
Morphometric Assessment

The purpose of the research is identifying a species of the causative agent of canine babesiosis in the Kirov Region.Materials and methods. To determine the parasite species, we microscopically examined smears from the peripheral blood of sick dogs stained by the Romanowsky-Giemsa method and Leucodif. The microscopy was performed with a binocular microscope Micromed-1. For morphometric assessment of the causative agent, we used a digital camera and software for image analysis ToupView.Results and discussion. We found parasites of various shapes and sizes in erythrocytes of the dogs’ blood in an amount from 1 to 4, rarely 8, 16, and 32. Parasites having large paired pear-shaped forms prevailed (100% in Kirov and 92.86% in Vyatskiye Polyany), which were mainly connected by thin ends at an acute angle and located in the center of an erythrocyte. In Vyatskiye Polyany, we found single small pear-shaped parasites in 7.14%, that were also located along the periphery of an erythrocyte. Based on morphological features, the causative agent of “large” Babesia infection (piroplasmosis) of dogs in the Kirov Region is Babesia (Piroplasma) canis (Piana et Galli Walerio, 1895), and of small Babesia infection of dogs is B. (P.) gibsoni (Patton, 1910). In the Kirov City environment, babesiosis proceeds in monoinfection (81.58% prevalence of infection), and the B. canis infection rate is 28.57% in the south of the Region. We recorded a mixed infection of B. canis + Anaplasma sp. (35.71%), B. canis + Anaplasma sp. + Ehrlichia canis (14.29%), B. canis + E. canis (14.29%), and B. gibsoni + Anaplasma sp. (7.14%) in 71.43%. 

Estimation of bodyweight from body measurements and determination of body measurements on Limousin cattle using digital image analysis

2016 ◽  
Vol 56 (12) ◽  
pp. 2060 ◽  
Author(s):  
Serkan Ozkaya ◽  
Wojciech Neja ◽  
Sylwia Krezel-Czopek ◽  
Adam Oler
Keyword(s):  
Image Analysis ◽  
Body Length ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Digital Camera ◽  
Body Measurements ◽  
Analysis Software ◽  
Body Area ◽  
Image Analysis Software

The objective of this study was to predict bodyweight and estimate body measurements of Limousin cattle using digital image analysis (DIA). Body measurements including body length, wither height, chest depth, and hip height of cattle were determined both manually (by measurements stick) and by using DIA. Body area was determined by using DIA. The images of Limousin cattle were taken while cattle were standing in a squeeze chute by a digital camera and analysed by image analysis software to obtain body measurements of each animal. While comparing the actual and predicted body measurements, the accuracy was determined as 98% for wither height, 97% for hip height, 94% for chest depth and 90.6% for body length. Regression analysis between body area and bodyweight yielded an equation with R2 of 61.5%. The regression equation, which included all body traits, resulted in an R2 value of 88.7%. The results indicated that DIA can be used for accurate prediction of body measurements and bodyweight of Limousin cattle.

Optical measurement of voids in situ during infusion of carbon reinforcements

2020 ◽  
pp. 002199832095982
Author(s):  
C Lystrup ◽  
A George ◽  
B Zobell ◽  
K Boster ◽  
C Childs ◽  
...  
Keyword(s):  
Image Analysis ◽  
Carbon Fiber ◽  
High Performance ◽  
Optical Measurement ◽  
Void Formation ◽  
Digital Camera ◽  
Void Content ◽  
Liquid Composite Molding ◽  
Automated Method

Liquid composite molding (LCM) is growing in importance as an alternative to traditional prepreg-autoclave methods for manufacture high-performance composites. The most significant roadblock to industry’s implementation of LCM is the usually higher void content compared with prepreg processing. One tool for reducing void levels in LCM involves optimization of flow velocity, which requires models to be developed to describe void formation at a given velocity. To help solve this problem, the following research illustrates the first known method for optical void measurement in situ during infusion in a carbon fiber reinforcement. Similar to previous studies on glass fiber, this work utilizes fluorescent dye and a digital camera to produce sufficient contrast and resolution for image analysis. Visible bubbles are photographed against the opaque carbon fiber background. An automated method of image analysis is outlined, which was used to analyze 230 images for three different flow orientations of a single fabric, producing the highest amount of experimental data seen so far on in situ void measurement. The resulting data identifies a minimum velocity threshold for minimal macro-void formation. The resultant void characterization framework will better enable optimization of LCM processing for high-performance composites based on carbon reinforcements.

Detection and molecular characterization of canine babesiosis causative agent Babesia canis in the naturally infected dog in Lithuania

2014 ◽  
Vol 205 (3-4) ◽  
pp. 702-706 ◽  
Author(s):  
Algimantas Paulauskas ◽  
Jana Radzijevskaja ◽  
Birutė Karvelienė ◽  
Aidas Grigonis ◽  
Asta Aleksandravičienė ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Causative Agent ◽  
Babesia Canis ◽  
Canine Babesiosis

scholarly journals Mixed haemosporidian infections are associated with higher fearfulness in Yellow Wagtail (Motacilla flava)

2017 ◽  
Vol 95 (6) ◽  
pp. 405-410 ◽  
Author(s):  
M.P. Marinov ◽  
C. Marchetti ◽  
D. Dimitrov ◽  
M. Ilieva ◽  
P. Zehtindjiev
Keyword(s):  
Mixed Infection ◽  
Parasite Species ◽  
Single Species ◽  
Blood Parasite ◽  
Activity Level ◽  
Single Infection ◽  
Novel Object ◽  
Yellow Wagtail ◽  
Parasite Infections ◽  
Single Genus

Blood parasite infections have been shown to influence behavioural traits of their avian hosts, in particular activity level and boldness. Following the hypothesis that a mixed infection by different parasite species should have higher effects than single-species infections, we analysed activity and boldness in wild-caught Yellow Wagtails (Motacilla flava L., 1758), a trans-Saharan migrant, during the energetically demanding spring migration. Eighty-five percent of the birds were naturally infected with Haemoproteus Kruse, 1890 or Plasmodium Marchiafava and Celli, 1885 (Sporozoa, Haemosporida) and 27% of individuals had parasites from both genera. No differences in activity were found among uninfected, single infection, and mixed infection groups. Birds with infections from both genera appeared to be more fearful when first introduced to a cage. These birds also tended to be less likely to approach a novel object compared with uninfected birds and birds infected by a single genus only.

scholarly journals Optimization of Binocular Microscope with Micro Digital Camera for Measuring Seminiferous Tubules Epithelium Height

2016 ◽  
Vol 5 (2) ◽  
pp. 41
Author(s):  
Sutriyono Sutriyono
Keyword(s):  
Science And Technology ◽  
Descriptive Analysis ◽  
Digital Camera ◽  
Mouse Testis ◽  
Seminiferous Tubules ◽  
Objective Lens ◽  
Ocular Lens ◽  
Binocular Microscope ◽  
The Mean ◽  
Biology Laboratory

Binocular microscope optimization using micro digital camera to measure seminiferous tubules epithelium of mouse testis (<em>Mus muculus</em>) was completed. This research was conducted at Biology Laboratory of Faculty of Science and Technology UIN Sunan Kalijaga. The purpose of this study was to determine the height of the seminiferous tubules epithelium of mouse testis using micro digital camera. A computer was connected with binocular microscope and optilab advance, then calibration of optilab advance were done on objective lens magnification at 4x, 10x and 40x with 10x ocular lens. The analysis used was descriptive analysis. The mean of  the seminiferous tubules epithelium height is 105.6 μm. Optimization on computer and binocular microscope with micro digital camera can be used to measure seminiferous tubules epithelium.

scholarly journals A Rapid and Efficient Method for Determination of Fruit Peel Color

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 842B-842
Author(s):  
Ignazio Infantino ◽  
Riccardo Lo Bianco*
Keyword(s):  
Image Analysis ◽  
Color Index ◽  
Visual Analysis ◽  
Color Image ◽  
Digital Camera ◽  
Critical Index ◽  
Fruit Peel ◽  
Traditional Methods ◽  
Peel Color

Peel color is a critical index of external fruit quality and consumer appreciation level. Traditional methods for determination of peel color are based on visual analysis or punctual measurements by colorimeter. In this study we present a method based on digital image analysis that integrates the accuracy of an interactive measurement and the efficacy of an image analysis that descibes entire sides of the fruit. A sample of apple, mandarin, grape, and peach fruit was photographed (each fruit on two opposite sides) with a digital camera for determination of peel color. Digital images were converted from RGB to CIE L*a*b* format, and color characteristics were indexed and quantified. The implemented method uses a reference color image cropped from the best fruit (interactively chosen) to calculate a color index for each fruit of the image set. The final index is the weighted sum of the number of pixels of the fruit, where pixels closer to the reference color (distance in the CIE L*a*b space) are considered more relevant. This color index gives integrated information on fruit color quantity (% of cover color) and quality (hue and saturation). The method represents a rapid and efficient way of determining color of the entire fruit surface and overcomes difficulties and approximation of traditional methods. A modification of the same method can be used to determine peel rugosity (mandarin) and average size and number of grape berries per bunch.

scholarly journals Image Analysis of Surface Porosity Mortar Containing Processed Spent Bleaching Earth

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1658
Author(s):  
Beng Wei Chong ◽  
Rokiah Othman ◽  
Ramadhansyah Putra Jaya ◽  
Doh Shu Ing ◽  
Xiaofeng Li ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Image Analysis ◽  
Water Absorption ◽  
Image Acquisition ◽  
Bleaching Earth ◽  
Digital Camera ◽  
Surface Porosity ◽  
Image Analysis Technique ◽  
Pozzolanic Material ◽  
Analysis Technique

Image analysis techniques are gaining popularity in the studies of civil engineering materials. However, the current established image analysis methods often require advanced machinery and strict image acquisition procedures which may be challenging in actual construction practices. In this study, we develop a simplified image analysis technique that uses images with only a digital camera and does not have a strict image acquisition regime. Mortar with 10%, 20%, 30%, and 40% pozzolanic material as cement replacement are prepared for the study. The properties of mortar are evaluated with flow table test, compressive strength test, water absorption test, and surface porosity based on the proposed image analysis technique. The experimental results show that mortar specimens with 20% processed spent bleaching earth (PSBE) achieve the highest 28-day compressive strength and lowest water absorption. The quantified image analysis results show accurate representation of mortar quality with 20% PSBE mortar having the lowest porosity. The regression analysis found strong correlations between all experimental data and the compressive strength. Hence, the developed technique is verified to be feasible as supplementary mortar properties for the study of mortar with pozzolanic material.

scholarly journals Mobility of nZVI in a Reconstructed Porous Media Monitored by an Image Analysis Procedure

Water ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 2797
Author(s):  
Francesca Andrei ◽  
Giuseppe Sappa ◽  
Maria Rosaria Boni ◽  
Giuseppe Mancini ◽  
Paolo Viotti
Keyword(s):  
Porous Media ◽  
Image Analysis ◽  
Groundwater Flow ◽  
Laboratory Experiment ◽  
Digital Camera ◽  
Calibration Procedure ◽  
Analysis Procedure ◽  
Zero Valent Iron ◽  
Third Dimension ◽  
Zero Valent Iron Nanoparticles

Zero-valent iron nanoparticle (nZVI) technology has been found to be promising and effective for the remediation of soils or groundwater. However, while nanoparticles are traveling through porous media, they can rapidly aggregate, causing their settling and deposition. When nZVI are injected in the groundwater flow, the behavior (mobility, dispersion, distribution) is unknown in groundwater, causing the use of enormous quantities of them if used at the field scale. In this paper, a laboratory experiment was carried out with groundwater flow in a two-dimensional, laboratory-scale tank to assess the nanoparticle behavior by means of an image analysis procedure. A solution of zero-valent iron nanoparticles, Nanofer 25S particles, were used and glass beads were utilized as porous medium. The laboratory experiment included the use of a digital camera for the acquisition of the images. The image analysis procedure was used to assess the behavior of nZVI plume. A calibration procedure and a mass balance were applied to validate the proposed image analysis procedure, with the hypothesis that nanoparticles would be uniformly distributed in the third dimension of the tank (thickness). The results show that the nanoparticles presented small dispersive effects and the motion was strongly influenced from the higher weight of them with respect to the water. Therefore, the results indicate that nanoparticles have an own motion not strongly influenced by the fluid flow but more determined from the injection phase and gravity. The statistical elaborations show that the nZVI plume did not respond to the classical mechanisms of the dispersion.

Image Compression in Morphometry Studies Requiring 21 CFR Part 11 Compliance: Procedure Is Key with TIFFs and Various JPEG Compression Strengths

2004 ◽  
Vol 32 (2) ◽  
pp. 258-263 ◽  
Author(s):  
Mark W. Tengowski
Keyword(s):  
Image Analysis ◽  
Image Compression ◽  
Digital Images ◽  
Digital Camera ◽  
Jpeg Compression ◽  
Work Flow ◽  
Analysis Software ◽  
Compressed Images ◽  
Electron Microscope Grid ◽  
Individual Data Point

This study aims to compare the integrity and reproducibility of measurements created from uncompressed and compressed digital images in order to implement compliance with 21 CFR Part 11 for image analysis studies executed using 21 CFR Part 58 compliant capture systems. Images of a 400-mesh electron microscope grid and H&E stained rat liver tissue were captured on an upright microscope with digital camera using commercially available analysis software. Digital images were stored as either uncompressed TIFFs or in one of five different levels of JPEG compression. The grid images were analyzed with automatic detection of bright objects while the liver images were segmented using color cube-based morphometry techniques, respectively, using commercially-available image analysis software? When comparing the feature-extracted measurements from the TIFF uncompressed to the JPEG compressed images, the data suggest that JPEG compression does not alter the accuracy or reliability to reproduce individual data point measurements in all but the highest compression levels. There is, however, discordance if the initial measure was obtained with a TIFF format and subsequently saved as one of the JPEG levels, suggesting that the use of compression must precede feature extraction. It is a common practice in software packages to work with TIFF uncompressed images. However, this study suggests that the use of JPEG compression as part of the analysis work flow was an acceptable practice for these images and features. Investigators applying image file compression to other organ images will need to validate the utility of image compression in their work flow. A procedure to digitally acquire and JPEG compress images prior to image analysis has the potential to reduce file archiving demands without compromising reproducibility of data.

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