scholarly journals The role of p16 / Ki-67 dual staining in HPV positive and negative women in the early diagnosis of cervical precancerous lesions: Cytology, colposcopy and conization protocol

2020 ◽  
Vol 41 (4) ◽  
pp. 569
Keyword(s):  
Early Diagnosis ◽  
Precancerous Lesions ◽  
Ki 67 ◽  
Cervical Precancerous Lesions ◽  
Dual Staining

scholarly journals Preliminary Study on the Expression of Testin, p16 and Ki-67 in the Cervical Intraepithelial Neoplasia

Biomedicines ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1010
Author(s):  
Aneta Popiel ◽  
Aleksandra Piotrowska ◽  
Patrycja Sputa-Grzegrzolka ◽  
Beata Smolarz ◽  
Hanna Romanowicz ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Precancerous Lesions ◽  
Clinical Stage ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Control Group ◽  
Ki 67 ◽  
Cervical Precancerous Lesions ◽  
P16 Expression

Cervical cancer is one of the most common malignant cancers in women worldwide. The 5-year survival rate is 65%; nevertheless, it depends on race, age, and clinical stage. In the oncogenesis of cervical cancer, persistent HPV infection plays a pivotal role. It disrupts the expression of key proteins as Ki-67, p16, involved in regulating the cell cycle. This study aimed to identify the potential role of testin in the diagnosis of cervical precancerous lesions (CIN). The study was performed on selected archival paraffin-embedded specimens of CIN1 (31), CIN2 (75), and CIN3 (123). Moderate positive correlation was observed between testin and Ki-67 as well as testin and p16 expression in all dysplastic lesions (r = 0.4209, r = 0.5681; p < 0.0001 for both). Statistical analysis showed stronger expression of the testin in dysplastic lesions vs. control group (p < 0.0001); moreover, expression was significantly higher in HSIL than LSIL group (p < 0.0024). In addition, a significantly stronger expression of testin was observed in CIN3 vs. CIN1 and CIN3 vs. CIN2. In our study, expression of Ki-67, p16, and testin increased gradually as the lesion progressed from LSIL to HSIL. The three markers complemented each other effectively, which may improve test sensitivity and specificity when used jointly.

Imperative role of early diagnosis in precancerous lesions: Oldideas, new findings, yet more questions

2012 ◽  
Vol 1 (4) ◽  
pp. 184 ◽  
Author(s):  
Prince Kumar ◽  
HarkanwalP Singh ◽  
Shallu Tyagi ◽  
Suresh Yadav ◽  
Roshni Dupare ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Precancerous Lesions ◽  
New Findings

scholarly journals Weipiling ameliorates gastric precancerous lesions in Atp4a−/− mice

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Wei Liu ◽  
Zi-ming Zhao ◽  
Yuan-liang Liu ◽  
Hua-feng Pan ◽  
Li-zhu Lin
Keyword(s):  
Gastric Mucosa ◽  
Precancerous Lesions ◽  
Tunel Staining ◽  
Model Group ◽  
Ki 67 ◽  
Protein Levels ◽  
Chinese Medicine Formula ◽  
Pas Staining ◽  
Different Doses

Abstract Background Altered cellular metabolism is considered to be one of the hallmarks of cancer (Coller, Am J Pathol 184:4–17, 2014; Kim and Bae, Curr Opin Hematol 25:52–59, 2018). However, few studies have investigated the role of metabolism in the development of gastric precancerous lesions (GPLs). Weipiling (WPL), a traditional Chinese medicine formula for treatment of GPLs. In this study, we evaluated the amelioration of GPLs by WPL and investigated the possible role of WPL in regulating glucose metabolism. Methods Firstly, the major components of WPL are chemically characterized by HPLC analytical method. In this study, we chose the Atp4a−/− mouse model (Spicer etal., J Biol Chem 275:21555–21565, 2000) for GPL analysis. Different doses of WPL were administered orally to mice for 10 weeks. Next, the pathological changes of gastric mucosa were assessed by the H&E staining and AB-PAS staining. In addition, TUNEL staining was used to evaluate apoptosis, and we further used immunohistochemically labelled CDX2, MUC2, ki-67, PTEN, and p53 proteins to assess the characteristic changes of gastric mucosa in precancerous lesions. The levels of such transporters as HK-II, PKM2, ENO1, MPC1, and LDHA were determined by Western blot analysis. Finally, we assessed the expression of mTOR, HIF-1α, AMPK, Rheb, TSC1 and TSC2 protein in the gastric mucosa of Atp4a−/−mice. Results In this work, we evaluated the protective effect of WPL on gastric mucosa in mice with precancerous lesions. The aberrant apoptosis in gastric mucosa of gastric pre-cancerous lesions was controlled by WPL (P<0.05). Furthermore, WPL suppressed the expression of CDX2, MUC2, ki-67, PTEN and p53, as the levels of these proteins decreased significantly compared with the model group (P<0.05). In parallel, WPL significantly suppressed the expression of transporters, such as HK-II, PKM2, ENO1, MPC1 and LDHA (P<0.05). In addition, mTOR, HIF-1a, AMPK, Rheb, TSC1 and TSC2 protein levels in gastric mucosa of Atp4a−/− mice in the high- and low-dose WPL groups were significantly lower than those in the model group (P<0.05), while the expression of TSC1 and TSC2 protein was significantly higher (P<0.05). Conclusions Conclusively, WPL could ameliorate GPLs in Atp4a−/− mice by inhibiting the expression of transporters and suppressing the aberrant activation of mTOR/HIF-1α.

Role of human papillomavirus (HPV) testing in the follow-up of patients after treatment for cervical precancerous lesions

2005 ◽  
Vol 118 (2) ◽  
pp. 229-234 ◽  
Author(s):  
Zoltán Hernádi ◽  
Krisztina Szőke ◽  
Tamás Sápy ◽  
Zoárd T. Krasznai ◽  
Györgyike Soós ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Precancerous Lesions ◽  
Hpv Testing ◽  
Cervical Precancerous Lesions

Role of Ancillary Techniques in Gynecologic Cytopathology Specimens

2019 ◽  
Vol 64 (1-2) ◽  
pp. 63-70
Author(s):  
Andrea D. Olivas ◽  
Julieta E. Barroeta ◽  
Ricardo R. Lastra
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cancer Screening ◽  
High Risk ◽  
Cervical Cancer Screening ◽  
Hpv Testing ◽  
Invasive Procedures ◽  
Ki 67 ◽  
Dual Staining

The association between high-risk genotypes of human papillomavirus (hr-HPV) and cervical cancer is well established. As hr-HPV testing is rapidly becoming a part of routine cervical cancer screening, either in conjunction with cytology or as primary testing, the management of hr-HPV-positive women has to be tailored in a way that increases the detection of cervical abnormalities while decreasing unnecessary colposcopic biopsies or other invasive procedures. In this review, we discuss the overall utility and strategies of hr-HPV testing, as well as the advantages and limitations of potential triage strategies for hr-HPV-positive women, including HPV genotyping, p16/Ki-67 dual staining, and methylation assays.

scholarly journals Diagnostic value of p16inkα/Ki-67 Dual-stained cytology in HPV-associated cervical diseases

2015 ◽  
Vol 64 (3) ◽  
pp. 47-51
Author(s):  
Armine Robertovna Khachaturyan ◽  
Ekaterina Konstantinovna Orekhova ◽  
Gulrukhsor Khaybulloevna Tolibova ◽  
Tatyana Georgiyevna Tral
Keyword(s):  
Human Papillomavirus ◽  
Early Diagnosis ◽  
Precancerous Lesions ◽  
Diagnostic Value ◽  
Hpv Infection ◽  
Ki 67 ◽  
Sexually Transmitted ◽  
Neoplastic Process ◽  
Process Detection ◽  
Cervical Diseases

Human papillomavirus (HPV) infection is currently considered the most common of sexually transmitted infections. However, only 8-20 % of cases of infection with human papillomavirus oncogenic risk has the potential to transform to a neoplastic process. Detection of productive forms of HPV infection is the basis of early diagnosis of precancerous lesions. In order to clarify the severity of neoplasia and choose a sufficient way of treatment we investigated the diagnostic value of p16inkα/Ki-67 Dual-stained cytology method for assessing the presence and degree of expression of p16inkα protein and Ki-67, biomarkers of virus-induced proliferation, the sensitivity and positive predictive value of co-expression degree of which can increase the diagnostic value of cervical cytology.

Human Papillomavirus Genotyping to Predict the Risk of Cervical Precancerous Lesions or Cancer in Women with Minor Abnormal Cytology in China

2015 ◽  
Vol 59 (5) ◽  
pp. 405-411 ◽  
Author(s):  
Chun-Qing Lin ◽  
Jian-Feng Cui ◽  
Xun Zhang ◽  
Qin-Jing Pan ◽  
Wen Chen ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Precancerous Lesions ◽  
Low Grade ◽  
Abnormal Cytology ◽  
Cervical Intraepithelial Neoplasia Grade ◽  
Cross Sectional ◽  
Hpv 16 ◽  
Hpv Genotyping ◽  
Cervical Precancerous Lesions

Objective: To evaluate the role of human papillomavirus (HPV) genotyping in predicting the risk of cervical precancerous lesions or cancer in women with minor abnormal cytology. Methods and Materials: This study was conducted on 329 women with atypical squamous cells of undetermined significance (ASC-US) and 77 women with low-grade squamous intraepithelial lesions (LSIL) out of a total of 4,215 participants in a multicenter, cross-sectional study. Liquid-based cytology and the Hybrid Capture 2 test (HC2) were used to screen eligible women, and a Linear Array HPV genotyping test was performed on women with positive HC2 results. Results: The sensitivity and specificity for detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2+) based on HPV 16/18 were 82% [95% confidence interval (CI): 52-95%] and 91% (95% CI: 87-94%) in women with ASC-US and 67% (95% CI: 35-88%) and 84% (95% CI: 73-91%) in women with LSIL. The women infected with HPV 16/18 had a significantly higher risk of developing CIN2+ than those infected with other high-risk HPV types in both the ASC-US (OR 9.93, 95% CI: 2.02-48.88) and LSIL (OR 7.45, 95% CI: 1.60-34.68) arms. Conclusions: Genotyping for HPV 16/18 greatly improves specificity, but at the expense of potential sensitivity in the triage of minor cytology abnormalities. The role of genotyping for HPV 16/18 in order to triage women with minor abnormal cytology should be further evaluated in future studies.

The role of immunohistochemistry in the diagnosis of cervical intraepithelial neoplasia of different severity

2016 ◽  
pp. 138-140
Author(s):  
S.I. Zhuk ◽  
◽  
O.A. Taran ◽  
A.N. Koshmienskaya ◽  
T.V. Lobastova ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cervical Intraepithelial Neoplasia ◽  
Precancerous Lesions ◽  
Molecular Genetic ◽  
Intraepithelial Neoplasia ◽  
Reproductive Age ◽  
Moderate Degree ◽  
Ki 67 ◽  
The Third ◽  
Diagnosis And Prognosis

The objective: the finding of protein expression of apoptosis regulator BCL-2, Smooth Muscule Actin and the antigen Ki-67 in cervical intraepithelial neoplasia of different severity to optimize the diagnosis and prognosis of the disease. Patients and methods. The study involved 42 women of reproductive age with cervical intraepithelial the neoplasia of the cervix varying degrees applied to the doctor of cervical pathology Zhitomir regional oncologic dispensary. All women (n=42) were divided into groups. The first group included 15 patients (35.7%) with cervical intraepithelial neoplasia with mild. The second group included 13 women (31%) with cervical intraepithelial neoplasia a moderate degree. The third group was represented by patients with cervical intraepithelial neoplasia with severe – 14 respondents (33.3 per cent). Results. Marker BCL-2 in patients of the first group was positive in 7 patients (46.7%), Smooth Muscule Actin was positive in 9 patients (60%) and Ki-67 was diagnosed in 8 of the surveyed women (53.3%). In the second group of BCL-2 was positive in 8 patients (61.5%), Clone 124, Smooth Muscule Actin, Clone 1A4 was positive in 9 patients (69.2%), and Ki-67 was diagnosed in 12 of the surveyed women (92.3%). Marker BCL-2 in patients of the third group was positive in 12 patients (85.7%), Smooth Muscule Actin was positive in 10 patients (71.4%) and Ki-67 was diagnosed in 13 of the surveyed women (92.9% ). Conclusion. Carcinogenesis is associated with molecular genetic damage to the cervix. Some of the products of this process can be used as prognostic and diagnostic markers of tumor progression. Determination of protein expression of apoptosis regulator BCL-2, Smooth Muscule Actin and the antigen Ki-67 in cervical intraepithelial neoplasia makes it possible to accurately verify the diagnosis and to predict the course of pathological changes in the flat epithelium of the cervix. Key words: cervical intraepithelial neoplasia, cervical cancer, morphological diagnostics of precancerous lesions, BCL-2, Smooth Muscule Actin, Ki-67.

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