Lysyl oxidase activity and synthesis of desmosines in cultured human aortic cells and skin fibroblasts: Comparison of cell lines from control subjects and patients with the Marfan syndrome or other annulo-aortic ectasia

1986 ◽  
Vol 46 (1) ◽  
pp. 31-37 ◽  
Author(s):  
T. Halme ◽  
T. Vihersaari ◽  
R. Penttinen
Keyword(s):  
Marfan Syndrome ◽  
Cell Lines ◽  
Oxidase Activity ◽  
Lysyl Oxidase ◽  
Skin Fibroblasts ◽  
Control Subjects ◽  
Aortic Ectasia

scholarly journals Reduced lysyl oxidase activity in skin fibroblasts from patients with Menkes' syndrome

1980 ◽  
Vol 192 (2) ◽  
pp. 579-586 ◽  
Author(s):  
P M Royce ◽  
J Camakaris ◽  
D M Danks
Keyword(s):  
Oxidase Activity ◽  
Culture Medium ◽  
Lysyl Oxidase ◽  
Skin Fibroblasts ◽  
Normal Fibroblast ◽  
Collagen Substrate ◽  
Fibroblast Cultures ◽  
Fibroblast Line ◽  
Cell Layers ◽  
Cellular Copper

Lysyl oxidase activity against both collagen and elastin substrates has been examined in the culture medium of skin fibroblasts derived from unrelated patients with Menkes' syndrome and from control subjects. The medium of three Menkes' fibroblast lines showed 3–30% of the activity present in the medium of control fibroblasts, against a purified collagen substrate. Lysyl oxidase activity in the culture medium of two of the Menkes' fibroblast lines was also examined by using a crude aortic-elastin substrate and was similarly decreased in comparison with that in the medium of control fibroblasts. Lysyl oxidase activity in the medium of a fourth fibroblast line, derived from a foetus with Menkes' syndrome, was 42% of that in the medium of control fibroblasts derived from a 1-day-old baby against a collagen substrate, and 26% of that in control fibroblast medium against an elastin substrate. The copper content of the cell layers of the Menkes' fibroblast cultures was elevated in comparison with normal fibroblast cultures, as has previously been reported to be characteristic of such cells. It is suggested that the decrease in lysyl oxidase activity would help to explain the connective tissue defects observed in Menkes' syndrome, and that this reduction, in conjunction with the elevated concentrations of cellular copper, would support the hypothesis that a functional intracellular copper deficiency exists in Menkes' syndrome.

scholarly journals Partial characterization of lysyl oxidase from several human tissues

1985 ◽  
Vol 230 (3) ◽  
pp. 639-643 ◽  
Author(s):  
H Kuivaniemi
Keyword(s):  
Human Skin ◽  
Oxidase Activity ◽  
Lysyl Oxidase ◽  
Sodium Dodecyl ◽  
Deae Cellulose ◽  
Active Species ◽  
Skin Fibroblasts ◽  
Human Tissues ◽  
Human Skin Fibroblasts ◽  
Tissue Samples

Lysyl oxidase activity was assayed in urea extracts of a number of human tissues, proving to be highest in skin. Antibodies to human placental lysyl oxidase completely inhibited the activity of crude lysyl oxidase from all the human tissues studied, with no significant differences in the amounts of antiserum required for 50% inhibition. By contrast, marked differences were found in this value between skin tissue samples from different species. The Mr of lysyl oxidase in crude extracts of human skin and in the medium of cultured human skin fibroblasts was 30 000 by gel filtration, no active species with a higher Mr being detectable. Four forms of lysyl oxidase activity were seen in DEAE-cellulose chromatography of urea extract from human skin, all having Mr 30 000. Antibodies to human placental lysyl oxidase stained a 30 000-Mr protein in urea extracts of all the human tissues studied and in the medium of cultured human skin fibroblasts when examined by immunoblotting after sodium dodecyl sulphate/polyacrylamide-slab-gel electrophoresis, but they also stained high-Mr material. The findings suggest that there are no immunologically distinct lysyl oxidase isoenzymes in the various human tissues and that the true Mr of lysyl oxidase in crude urea extracts is 30 000.

Dietary Copper, Vanadate, Lysyl Oxidase Activity, and Lysine Tyrosyl Quinone Formation

2002 ◽  
pp. 929-932
Author(s):  
Robert B. Rucker ◽  
Changtai Cui ◽  
Eskouhie H. Tchaparian ◽  
Alyson E. Mitchell ◽  
Michael Clegg ◽  
...  
Keyword(s):  
Oxidase Activity ◽  
Lysyl Oxidase ◽  
Dietary Copper ◽  
Copper Vanadate

Generation of Fluorescently Labeled Cell Lines, C3A Hepatoma Cells, and Human Adult Skin Fibroblasts to Study Coculture Models

2013 ◽  
Vol 37 (7) ◽  
pp. E123-E130 ◽  
Author(s):  
Anna Samluk ◽  
Karolina Ewa Zakrzewska ◽  
Krzysztof Dariusz Pluta
Keyword(s):  
Cell Lines ◽  
Hepatoma Cells ◽  
Skin Fibroblasts ◽  
Human Adult ◽  
Adult Skin

scholarly journals N-Acetylcysteine Downregulation of Lysyl Oxidase Activity Alleviating Bleomycin-Induced Pulmonary Fibrosis in Rats

Respiration ◽  
2012 ◽  
Vol 84 (6) ◽  
pp. 509-517 ◽  
Author(s):  
Shifeng Li ◽  
Xiaoxiao Yang ◽  
Wande Li ◽  
Jingjie Li ◽  
Xingwen Su ◽  
...  
Keyword(s):  
Pulmonary Fibrosis ◽  
Oxidase Activity ◽  
Lysyl Oxidase

scholarly journals Mutual Expression of ALDH1A1, LOX, and Collagens in Ovarian Cancer Cell Lines as Combined CSCs- and ECM-Related Models of Drug Resistance Development

2018 ◽  
Vol 20 (1) ◽  
pp. 54 ◽  
Author(s):  
Karolina Sterzyńska ◽  
Andrzej Klejewski ◽  
Karolina Wojtowicz ◽  
Monika Świerczewska ◽  
Marta Nowacka ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Extracellular Matrix ◽  
Drug Resistance ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Lysyl Oxidase ◽  
Resistant Cell ◽  
Pcr Analysis ◽  
Ovarian Cancer Cells

A major contributor leading to treatment failure of ovarian cancer patients is the drug resistance of cancer cell. CSCs- (cancer stem cells) and ECM (extracellular matrix)-related models of drug resistance are described as independently occurring in cancer cells. Lysyl oxidase (LOX) is another extracellular protein involved in collagen cross-linking and remodeling of extracellular matrix and has been correlated with tumor progression. The expression of LOX, COL1A2, COL3A1, and ALDH1A1 was performed in sensitive (A2780, W1) and resistant to paclitaxel (PAC) (A2780PR1 and W1PR2) and topotecan (TOP) (W1TR) cell lines at the mRNA (real-time PCR analysis) and protein level (Western blot and immunofluorescence analysis). The ALDH1A1 activity was measured with the ALDEFLUOR test and flow cytometry analysis. The protein expression in ovarian cancer tissues was determined by immunohistochemistry. We observed an increased expression of LOX and collagens in PAC and TOP resistant cell lines. Subpopulations of ALDH1A1 positive and negative cells were also noted for examined cell lines. Additionally, the coexpression of LOX with ALDH1A1 and COL1A2 with ALDH1A1 was observed. The expression of LOX, collagens, and ALDH1A1 was also detected in ovarian cancer lesions. In our study LOX, ALDH1A1 and collagens were found to be coordinately expressed by cells resistant to PAC (LOX, ALDH1A1, and COL1A2) or to TOP (LOX and ALDH1A1). This represents the study where molecules related with CSCs (ALDH1A1) and ECM (LOX, collagens) models of drug resistance are described as occurring simultaneously in ovarian cancer cells treated with PAC and TOP.

Sign in / Sign up

Export Citation Format

Share Document