Effect of successive administration of zopiclone, a new class of minor tranquillizer, on mouse liver microsomal mono-oxygenase system

Bismuth (Bi) stains nucleoproteins (NPs) by interacting with available amino and primary phosphate groups. These two staining mechanisms are distinguishable by glutaraldehyde crosslinking (Fig. 1,2).Isolated mouse liver nuclei, extracted with salt and acid solutions, fixed in either formaldehyde (form.) or gl utaraldehyde (glut.) and stained with Bi, were viewed to determine the effect of the extractions on Bi stainina. Solubilized NPs were analyzed by SDS-polyacrylamide gel electrophoresis.Extraction with 0.14 M salt does not change the Bi staining characteristics (Fig. 3). 0.34 M salt reduces nucleolar (Nu) staining but has no effect on interchromatinic (IC) staining (Fig. 4). Proteins responsible for Nu and glut.- insensitive IC staining are removed when nuclei are extracted with 0.6 M salt (Fig. 5, 6). Low salt and acid extraction prevents Bi-Nu staining but has no effect on IC staining (Fig. 7). When nuclei are extracted with 0.6 M salt followed by low salt and acid, all Bi-staining components are removed (Fig. 8).

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Site of Lysosome Production During Hepatic Injury

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100063652 ◽

1969 ◽

Vol 27 ◽

pp. 348-349

Author(s):

Nalin J. Unakar

Keyword(s):

Electron Microscopy ◽

Golgi Apparatus ◽

Mouse Liver ◽

Hepatic Injury ◽

Close Approximation ◽

Experimental Conditions ◽

Toxic Injury

The increased number of lysosomes as well as the close approximation of lysosomes to the Golgi apparatus in tissue under variety of experimental conditions is commonly observed. These observations suggest Golgi involvement in lysosomal production. The role of the Golgi apparatus in the production of lysosomes in mouse liver was studied by electron microscopy of liver following toxic injury by CCI4.

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In Situ microscopy of the anodic etching of silicon

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100137537 ◽

1995 ◽

Vol 53 ◽

pp. 232-233

Author(s):

Frances M. Ross ◽

Peter C. Searson

Keyword(s):

Composite Structures ◽

High Surface ◽

High Surface Area ◽

Chemical Properties ◽

Selective Etching ◽

Silicon On Insulator ◽

Second Phase ◽

Porous Layers ◽

New Class ◽

Porous Semiconductors

Porous semiconductors represent a relatively new class of materials formed by the selective etching of a single or polycrystalline substrate. Although porous silicon has received considerable attention due to its novel optical properties1, porous layers can be formed in other semiconductors such as GaAs and GaP. These materials are characterised by very high surface area and by electrical, optical and chemical properties that may differ considerably from bulk. The properties depend on the pore morphology, which can be controlled by adjusting the processing conditions and the dopant concentration. A number of novel structures can be fabricated using selective etching. For example, self-supporting membranes can be made by growing pores through a wafer, films with modulated pore structure can be fabricated by varying the applied potential during growth, composite structures can be prepared by depositing a second phase into the pores and silicon-on-insulator structures can be formed by oxidising a buried porous layer. In all these applications the ability to grow nanostructures controllably is critical.

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The microtubule associated protein (MAP) tau forms a new class of triple-stranded left-hand helical fibrous protein polymer

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123106 ◽

1992 ◽

Vol 50 (1) ◽

pp. 540-541

Author(s):

G. C. Ruben ◽

K. Iqbal ◽

I. Grundke-Iqbal ◽

H. Wisniewski ◽

T. L. Ciardelli ◽

...

Keyword(s):

Axial Ratio ◽

Normal Structure ◽

Paired Helical Filaments ◽

Left Hand ◽

New Class ◽

Protein Polymer ◽

Fibrous Protein ◽

Protein Map ◽

Neurotransmitter Transport ◽

Alzheimer Neurofibrillary Tangles

In neurons, the microtubule associated protein, tau, is found in the axons. Tau stabilizes the microtubules required for neurotransmitter transport to the axonal terminal. Since tau has been found in both Alzheimer neurofibrillary tangles (NFT) and in paired helical filaments (PHF), the study of tau's normal structure had to preceed TEM studies of NFT and PHF. The structure of tau was first studied by ultracentrifugation. This work suggested that it was a rod shaped molecule with an axial ratio of 20:1. More recently, paraciystals of phosphorylated and nonphosphoiylated tau have been reported. Phosphorylated tau was 90-95 nm in length and 3-6 nm in diameter where as nonphosphorylated tau was 69-75 nm in length. A shorter length of 30 nm was reported for undamaged tau indicating that it is an extremely flexible molecule. Tau was also studied in relation to microtubules, and its length was found to be 56.1±14.1 nm.

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Study of segregation in La1.8Sr0.2CuO4 superconductor by STEM-EDS microanalysis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100125294 ◽

1987 ◽

Vol 45 ◽

pp. 54-55

Author(s):

T. F. Kelly ◽

P. J. Lee ◽

E. E. Hellstrom ◽

D. C. Larbalestier

Keyword(s):

Rare Earth ◽

High Field ◽

Transport Current ◽

Total Thickness ◽

New Class ◽

Ceramic Superconductors ◽

Current Densities ◽

Group Ii ◽

Eds Microanalysis

Recently there has been much excitement over a new class of high Tc (>30 K) ceramic superconductors of the form A1-xBxCuO4-x, where A is a rare earth and B is from Group II. Unfortunately these materials have only been able to support small transport current densities 1-10 A/cm2. It is very desirable to increase these values by 2 to 3 orders of magnitude for useful high field applications. The reason for these small transport currents is as yet unknown. Evidence has, however, been presented for superconducting clusters on a 50-100 nm scale and on a 1-3 μm scale. We therefore planned a detailed TEM and STEM microanalysis study in order to see whether any evidence for the clusters could be seen.A La1.8Sr0.2Cu04 pellet was cut into 1 mm thick slices from which 3 mm discs were cut. The discs were subsequently mechanically ground to 100 μm total thickness and dimpled to 20 μm thickness at the center.

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Nuclear coiled bodies and the nucleolus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100167834 ◽

1994 ◽

Vol 52 ◽

pp. 20-21

Author(s):

K. Brasch ◽

J. Williams ◽

D. Gallo ◽

T. Lee ◽

R. L. Ochs

Keyword(s):

Mouse Liver ◽

Nuclear Body ◽

Nuclear Bodies ◽

Model Systems ◽

Coiled Body ◽

Rrna Processing ◽

Malignant Cells ◽

Sm Proteins ◽

Coiled Bodies ◽

Unique Protein

Though first described in 1903 by Ramon-y-Cajal as silver-staining “accessory bodies” to nucleoli, nuclear bodies were subsequently rediscovered by electron microscopy about 30 years ago. Nuclear bodies are ubiquitous, but seem most abundant in hyperactive and malignant cells. The best studied type of nuclear body is the coiled body (CB), so termed due to characteristic morphology and content of a unique protein, p80-coilin (Fig.1). While no specific functions have as yet been assigned to CBs, they contain spliceosome snRNAs and proteins, and also the nucleolar protein fibrillarin. In addition, there is mounting evidence that CBs arise from or are generated near the nucleolus and then migrate into the nucleoplasm. This suggests that as yet undefined links may exist, between nucleolar pre-rRNA processing events and the spliceosome-associated Sm proteins in CBs.We are examining CB and nucleolar changes in three diverse model systems: (1) estrogen stimulated chick liver, (2) normal and neoplastic cells, and (3) polyploid mouse liver.

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Electronic structure studies of conducting polymers by electron energy-loss spectroscopy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100163265 ◽

1996 ◽

Vol 54 ◽

pp. 160-161

Author(s):

J. Fink

Keyword(s):

Electronic Structure ◽

Conducting Polymers ◽

Conjugated Polymers ◽

Electron Acceptors ◽

Electron Donors ◽

Light Emitting ◽

One Dimensional ◽

New Class ◽

Electrical Conductivities ◽

Electron Energy Loss

Conducting polymers comprises a new class of materials achieving electrical conductivities which rival those of the best metals. The parent compounds (conjugated polymers) are quasi-one-dimensional semiconductors. These polymers can be doped by electron acceptors or electron donors. The prototype of these materials is polyacetylene (PA). There are various other conjugated polymers such as polyparaphenylene, polyphenylenevinylene, polypoyrrole or polythiophene. The doped systems, i.e. the conducting polymers, have intersting potential technological applications such as replacement of conventional metals in electronic shielding and antistatic equipment, rechargable batteries, and flexible light emitting diodes.Although these systems have been investigated almost 20 years, the electronic structure of the doped metallic systems is not clear and even the reason for the gap in undoped semiconducting systems is under discussion.

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Mechanochemical changes on cyclometalated Ir(iii) acyclic carbene complexes – design and tuning of luminescent mechanochromic transition metal complexes

Inorganic Chemistry Frontiers ◽

10.1039/c9qi01278h ◽

2020 ◽

Vol 7 (3) ◽

pp. 786-794 ◽

Cited By ~ 3

Author(s):

Jingqi Han ◽

Kin-Man Tang ◽

Shun-Cheung Cheng ◽

Chi-On Ng ◽

Yuen-Kiu Chun ◽

...

Keyword(s):

Transition Metal ◽

Metal Complexes ◽

Transition Metal Complexes ◽

Carbene Complexes ◽

New Class

A new class of luminescent cyclometalated Ir(iii) complexes with readily tunable mechanochromic properties derived from the mechanically induced trans-to-cis isomerization have been developed.

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