scholarly journals Characterization of culturable bacterial communities associated with cage cultured fish under different stocking densities in Setiu Wetlands, Terengganu, Malaysia

2021 ◽  
Author(s):  
Mohammed M.Tawfeeq ◽  
Mohd Effendy Abd. Wahid ◽  
Musa Najiah ◽  
Isabelle George
Keyword(s):  
Bacterial Species ◽  
Drug Application ◽  
Stocking Density ◽  
Antibiotic Sensitivity ◽  
Water Clarity ◽  
Quality Parameters ◽  
Study Objective ◽  
Significant Difference ◽  
Fish Samples ◽  
High Fish

The study objective was to evaluate the effects of fish stocking density on the prevalence of pathogens isolated from sixty clinically healthy fishes reared under different densities in floating net-cages in Setiu lagoon, Terengganu state, Malaysia. The water temperature, pH, DO, TDS, salinity, water clarity, depth, and coliform concentration, were all determined within the fish cages. The healthy fish samples were randomly collected from 3 sites in cages with low and high stocking densities at each site. The bacteria were isolated from the skin, gills, kidneys, and liver of each fish sample, followed by identification to species level using the VITEK-2 system.The pathogens with beta-hemolysis characteristics were selected for antibiotic susceptibility against the following drugs: AM-10 μg, P-10 U, CL-30 μg, TC-30 μg, CP-5 μg, GM-10 μg, KM-30μg, and SM-10 μg. Water quality parameters showed no differences between the cages of low and high fish densities except for site C. The total number of isolates, microbial species, and the number of pathogens isolated from fish revealed no significant difference between the fish stocked in low and high densities. A total of 25 bacterial species were isolated, which included 14 gram-positive and 11 gram-negative. The (SM) drug application is suspected on this farm. The highest number of isolates was Staphylococcus warneri (15.90%), and Staphylococcus sciuri (14.77%). Most tested pathogens exhibited susceptibility to the selected drugs, but variation in the antibiotic sensitivity was observed between the isolates from low and high fish densities. The most potent drug was (CP), while (SM) application was suspected on this farm.

scholarly journals Characterization of culturable bacterial communities associated with cage cultured fish under different stocking densities in Setiu Wetlands, Terengganu, Malaysia.

2021 ◽  
Author(s):  
Mohammed Salah M.Tawfeeq
Keyword(s):  
Bacterial Species ◽  
Drug Application ◽  
Stocking Density ◽  
Water Clarity ◽  
Quality Parameters ◽  
Study Objective ◽  
Significant Difference ◽  
Fish Samples ◽  
Cultured Fish ◽  
Net Cages

Abstract The study objective was to evaluate the effects of fish stocking density on the prevalence of pathogens isolated from sixty clinically healthy fishes reared under different densities in floating net-cages in Setiu lagoon. The water temperature, pH, DO, TDS, salinity, water clarity, depth, and coliform concentration, were all determined within the fish cages. The healthy fish samples were randomly collected from 3 sites in cages with low and high stocking densities at each site. The bacteria were isolated from the skin, gills, kidneys, and liver of each fish sample, followed by identification to species level using the VITEK-2 system. The pathogens with beta-hemolysis characteristics were selected for antibiotic susceptibility against the following drugs: AM-10 µg, P-10 U, CL-30 µg, TC-30 µg, CP-5 µg, GM-10 µg, KM-30µg, and SM-10 µg. Water quality parameters showed no differences between the cages of low and high fish densities except for site C. The total number of isolates, microbial species, and the number of pathogens isolated from fish revealed no significant difference between the fish stocked in low and high densities. A total of 25 bacterial species were isolated, which included 14 gram-positive and 11 gram-negative. The (SM) drug application is suspected on this farm.

scholarly journals Comparison of Performance between Organic Farming and Traditional Farming of Freshwater Prawn in Respect of Production and Economics

2016 ◽  
Vol 9 (1) ◽  
pp. 109-116
Author(s):  
MR Haque ◽  
N Nahar ◽  
R Pervin ◽  
MA Samad
Keyword(s):  
Organic Farming ◽  
C:N Ratio ◽  
Total Yield ◽  
Stocking Density ◽  
Quality Parameters ◽  
Freshwater Prawn ◽  
Cost Ratio ◽  
Feed Conversion ◽  
Traditional Farming ◽  
Significant Difference

An experiment was conducted to compare the performance between traditional farming and organic farming of freshwater prawn in respect of production and economics. The experiment had two treatments, namely: Traditional (TT) and Organic (TO) with same stocking density (20000 juvenile’s ha-1) in triplicate. In treatment TO, formulated feed with locally available ingredients were used, and maize flour and bamboo branches were provided for maintaining C:N ratio to 20 and for developing periphyton, respectively. In treatment TT, only commercially available prawn feed was applied. No fertilizers or other types of chemicals or antibiotics were used in both treatments. There were no significant differences (P?0.05) of different water quality parameters between both treatments. Weight gain and specific growth rate (% body weight day-1) of prawn was significantly higher (P<0.05) in TO than TT treatment. Survival rate and feed conversion ratio of prawn did not vary significantly between both treatments. Highest total yield was obtained from TO treatment (511.41 kg ha-1 90 d-1) compared to TT treatment (426.57 kg ha-1 90 d-1). Net yield was also significantly higher in treatment TO (428.32 kg ha-1 90 d-1) than TT (347.77 kg ha-1 90-1). Although there was no significant difference in benefit-cost ratio between both treatments, totalreturn was significantly higher in TO than TT treatment.J. Environ. Sci. & Natural Resources, 9(1): 109-116 2016

scholarly journals Optimization of stocking rates of tiger shrimp under modified improved culture system

1970 ◽  
Vol 6 (2) ◽  
pp. 341-348 ◽  
Author(s):  
ML Islam ◽  
MJ Alam
Keyword(s):  
Water Quality ◽  
Bacterial Load ◽  
Penaeus Monodon ◽  
Stocking Density ◽  
Quality Parameters ◽  
Water Quality Parameters ◽  
Shrimp Culture ◽  
Tiger Shrimp ◽  
Significant Difference ◽  
Initial Deposition

With the target of increasing the shrimp (Penaeus monodon) production through farm development and improved grow out management, an experiment was conducted with three different stocking densities viz. 5/m2 (T1), 7/m2 (T2) and 9/m2 (T3). Results of the experiment revealed that, among the water quality parameters, values of dissolved oxygen and transparency were lower in the higher stocking densities with significant difference (p<0.05) among the treatments. Other water quality parameters were similar in all the treatments. Bacterial load in bottom sediment had the trend to increase with increasing culture period and showed significant difference (p<0.05) among the treatments with the highest in the higher stocking density. Soil pH in the end sample reduced a little bit in all the treatments than the initial. Deposition of organic matter, phosphorus and nitrogen was related to the respective stocking densities. Final weight gain and survival rate of shrimp were significantly (p<0.05) higher in T1 than in T2 and T3. Stocking density of 5/m2 showed the highest production of 759.14±19.002 kg/ha, which was significantly (P<0.05) higher than that of 670.77±16.078 kg/ha obtained in T2, but similar to 701.24±33.507 kg/ha obtained in T3. The net economic return and BCR was also significantly higher (p<0.05) in the same stocking density (5/m2) compared to others. Whereas, FCR value was significantly lower (p<0.05) in T1 than the other two treatments. However, pond ecological conditions were more congenial for shrimp culture in lower stocking density (5/m2), which minimized the risk of disease outbreak in the respective stocking density, keeping the culture environment favorable. Key words: Stocking density; Tiger shrimp; Modified improved culture DOI: 10.3329/jbau.v6i2.4832 J. Bangladesh Agril. Univ. 6(2): 341-348, 2008

scholarly journals Bacteriological Quality of Marketed Mola Fish, Amblypharyngodon Mola From Dhaka Metropolis

2012 ◽  
Vol 40 (1) ◽  
pp. 77-88 ◽  
Author(s):  
Saima Sharif Nilla ◽  
Md Anisur Rahman Khan ◽  
Md Mahmudur Rahman Khan ◽  
Dewan Ali Ahsan ◽  
Md Ghulam Mustafa
Keyword(s):  
Antibiotic Sensitivity ◽  
Bacterial Count ◽  
Quality Parameters ◽  
Quality Analysis ◽  
Total Bacterial Count ◽  
Local Market ◽  
Bacteriological Quality ◽  
Sensitivity Pattern ◽  
Fish Samples

The bacteriological quality of mola fish (Amblypharyngodon mola) from three local fish markets as fresh and as frozen from three departmental chain shops of Dhaka metropolis were analyzed. The microbial quality parameters varied with different sources and the quality was found to be poor for local market fish samples. In total 24 samples were considered for bacteriological quality analysis and 10 pathogenic isolates for antibiotic sensitivity test to 12 antibiotics. The total bacterial count ranged from 1.8 ± 0.25×104 to 6.5 ± 0.75 × 106 cfu/g for fresh and 5.5 ± 0.55 × 103 to 7.0 ± 0.80 × 105 cfu/g for frozen mola. The highest total coliform count of mola was 8.0 ± 0.55 × 104 and 6.1 ± 0.40 × 103 cfu/g for local market and departmental chain shop, respectively. All fresh and frozen samples were observed having high quantity of E. coli above 102 cfu/g. Furthermore, Salmonella-Shigella was identified in 67% samples (75% of fresh and 58% of frozen  samples) varied from 0.9 ± 0.00 ×102 to 5.3 ± 0.30 × 103 cfu/g whereas Vibrio spp.was confirmed in 79% samples (83% of fresh and 75% of frozen samples) of which  90% samples exceeded 102 cfu/g. Similar pattern was observed in Staphylococcus spp. with 83% of fresh and 58% of frozen samples (63% of total samples) beyond 103 cfu/g. In case of antibiotic sensitivity pattern of the indicator and pathogenic isolates, all of them were resistant to amoxicillin and penicillin. Most of the isolates were sensitive to bacitracin, ciprofloxacin, erythromycin and streptomycin. The findings proved that mola fish under this study was more or less  contaminated and local fish market samples were highly privileged with food borne pathogens which confirmed the unhygienic condition of the market as well as the  presence of antibiotic resistance bacteria in mola fish may threat to public health safety. DOI: http://dx.doi.org/10.3329/bjz.v40i1.12897 Bangladesh J. Zool. 40(1): 77-88, 2012

scholarly journals Fish Diversity and Water Quality Parameters of Mechi River, Jhapa, Province No. 1, Nepal

2021 ◽  
Vol 11 (1) ◽  
pp. 24-34
Author(s):  
ASHIM ADHIKARI ◽  
JASH HANG LIMBU ◽  
SOMIKA PATHAK
Keyword(s):  
Fish Community ◽  
Mesh Size ◽  
Quality Parameters ◽  
Fish Diversity ◽  
Fish Assemblage Structure ◽  
Fish Community Structure ◽  
Brachydanio Rerio ◽  
Significant Difference ◽  
Fish Samples ◽  
Spatio Temporal

Species diversity is widely recognised as an important trait of functioning and resilient ecosystem. Spatio-temporal patterns of fish community structure in the Mechi River was studied based on stratified random survey, conducted between September 2018 and April 2019. The survey was conducted at three different sampling stations in three different seasons. For the fish sampling, two cast nets of different sizes were used, one having large mesh size of 0.5 cm, 3 m diameter and 2 kg weight and another having mesh size of 2 cm, 6 m diameter and 6 kg weight. In addition, drag net and gill net were also used to collect the fish samples. A total of 1,772 fishes belonging to 4 orders, 8 families, 16 genera and 33 species were documented. The results from the similarity percentage analysis indicated that, the species Schistura devdevi, Brachydanio rerio, Puntius sophore, Barilius barila, Schistura beveani and Puntius terio were the most copious fish species recorded from the Mechi River. One-way analysis of similarity testing for space and time variations in fish community indicated that there was a significant difference in space (R = 0.66, p<0.01) but no significant difference in time (R = -0.0123, p>0.433). The Canonical Correspondence Analysis hinted that dissolved oxygen, water velocity and pH have shown to influence the fish assemblage structure in Mechi River.

Assessment of Bacterial Contamination of Orthodontic Arch wire

2019 ◽  
Vol 31 (1) ◽  
pp. 48-51
Author(s):  
Suha S Hassan ◽  
Nidhal H. Ghaib ◽  
Batool H Al-Ghurabi
Keyword(s):  
Bacterial Contamination ◽  
Microbial Contamination ◽  
Ethylenediaminetetraacetic Acid ◽  
Bacterial Species ◽  
Brain Heart Infusion ◽  
Control Groups ◽  
Dental Practitioners ◽  
Significant Difference ◽  
Bacillus Spp ◽  
Droplet Transmission

Background: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manufactures are free from microbial contamination and to determine the bacterial species attached to the arch wires. Materials and Methods: This study involved eighty samples, consisted of two types of arch wires (nitinol and stainless-steel) from four companies (3M, G&H, Jiscop, OrthoTechnology). These wires inserted in a plane tube that contains 10 -ml of (Tris [tris(hydroxymethyl)aminomethane] and EDTA (ethylenediaminetetraacetic acid) tris-EDTA and brain heart infusion (BHI) broth. A 0.1 ml was withdrawn from the tube and spread on agar plates. The control groups consist of 16 plane tube (8 tubes with tris-EDTA and other 8 tubes with (BHI). Results: Microbial sampling yielded growth from 5 of the 80 arch wires. The predominant bacteria that isolated were Bacillus spp. No growth was recovered from 75 of the samples and from controls. The bacteria were isolated by BHI reagent and no growth was observed by tris-EDTA reagent with statistically significant difference (P<0.05). The Bacillus spp. found only in the G&H and Jiscop companies, however, no statistically significant difference was found among them (P>0.05). With regard to the presence and distribution of bacteria according to the types of wires, the present results clarified that cases of contamination with Bacillus spp. were found in the nitinol arch wires with statistically significant difference (P<0.05). Conclusions: The results of the current study revealed low count of bacterial contamination in the two types of companies (G&H and Jiscop). Not all materials that received from the manufactures are free from contamination and an effective sterilization regimen is needed to avoid cross-contamination.

scholarly journals Utility of the optical quality analysis system for decision-making in Nd: YAG laser posterior capsulotomy in patients with light posterior capsule opacity

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Bo Lu ◽  
Weijie Zhu ◽  
Yu Fan ◽  
Dong Shi ◽  
Liwei Ma
Keyword(s):  
Area Under The Curve ◽  
Quality Parameters ◽  
Optical Quality ◽  
Quality Analysis ◽  
Posterior Capsule ◽  
Clinical Trial Registry ◽  
Posterior Capsulotomy ◽  
Significant Difference ◽  
Analysis System ◽  
Scattering Index

Abstract Background A prospective cohort study was performed to evaluate whether the Optical Quality Analysis System (OQAS) can serve as a valuable additional indicator for appropriate posterior capsulotomy referral. Methods One hundred and five eyes from 96 patients undergoing capsulotomy were divided into precapsulotomy logMAR CDVA ≤0.1 group and logMAR CDVA > 0.1 group. CDVA, and the Visual Function 14 index (VF-14) score were estimated before and 1 month after capsulotomy. The objective scattering index (OSI) value was measured by using the OQAS. Posterior capsule opacification (PCO) severity was assessed with Evaluation of PCO 2000 (EPCO 2000) software. Results In logMAR CDVA > 0.1 group, the correlations of OSI, logMAR CDVA, EPCO score and VF-14 score were very strong preoperatively. In logMAR CDVA ≤0.1 group, preoperatively, OSI was correlated with logMAR CDVA (r = 0.451), EPCO score (r = 0.789), and VF-14 score (r = 0.852). LogMAR CDVA has weak correlation with VF-14 score (r = − 0.384) and EPCO score (r = 0.566). VF-14 score was correlated with EPCO score (r = − 0.669). In the logMAR CDVA ≤0.1 group, there was no significant difference in logMAR CDVA between precapsulotomy and postcapsulotomy (P > 0.05). In the two groups, all the other optical quality parameters were significantly improved after capsulotomy (P < 0.05). In logMAR CDVA > 0.1 group, the area under the curve of the ROC of the OSI was 0.996 (P = 0.000). In logMAR CDVA ≤0.1 group, the area under the curve of the ROC of the OSI was 0.943 (P = 0.000). Conclusions The OSI was useful for evaluating of PCO and prediction of beneficial capsulotomy. Especially for patients with slight PCO and better visual acuity, OSI is more valuable than CDVA and completely objective examination. Trial registration The study protocol was registered at the Chinese Clinical Trial Registry. Register: ChiCTR1800018842 (Registered Date: October 13th, 2018).

scholarly journals Evaluation of Minimum Preparation Sampling Strategies for Sugarcane Quality Prediction by vis-NIR Spectroscopy

Sensors ◽  
2021 ◽  
Vol 21 (6) ◽  
pp. 2195
Author(s):  
Lucas de Paula Corrêdo ◽  
Leonardo Felipe Maldaner ◽  
Helizani Couto Bazame ◽  
José Paulo Molin
Keyword(s):  
Sample Preparation ◽  
Prediction Models ◽  
Spectral Response ◽  
Nir Spectroscopy ◽  
Quality Parameters ◽  
Partial Least Square ◽  
Least Square ◽  
Sample Type ◽  
Sampling Strategies ◽  
Significant Difference

Proximal sensing for assessing sugarcane quality information during harvest can be affected by various factors, including the type of sample preparation. The objective of this study was to determine the best sugarcane sample type and analyze the spectral response for the prediction of quality parameters of sugarcane from visible and near-infrared (vis-NIR) spectroscopy. The sampling and spectral data acquisition were performed during the analysis of samples by conventional methods in a sugar mill laboratory. Samples of billets were collected and four modes of scanning and sample preparation were evaluated: outer-surface (‘skin’) (SS), cross-sectional scanning (CSS), defibrated cane (DF), and raw juice (RJ) to analyze the parameters soluble solids content (Brix), saccharose (Pol), fibre, pol of cane and total recoverable sugars (TRS). Predictive models based on Partial Least Square Regression (PLSR) were built with the vis-NIR spectral measurements. There was no significant difference (p-value > 0.05) between the accuracy SS and CSS samples compared to DF and RJ samples for all prediction models. However, DF samples presented the best predictive performance values for the main sugarcane quality parameters, and required only minimal sample preparation. The results contribute to advancing the development of on-board quality monitoring in sugarcane, indicating better sampling strategies.

Effects of Different Stocking Densities on Growth, Serum Steroid Hormone Concentrations, Gonadosomatic Index and Sex Reversal in the Rice Field Eel, Monopterus albus (Zuiew)

2011 ◽  
Vol 142 ◽  
pp. 233-237
Author(s):  
Han Wen Yuan ◽  
Fang Chen ◽  
Qiao Qing Xu ◽  
Shi Yuan Gong ◽  
Zhang Jie Chu ◽  
...  
Keyword(s):  
Rice Field ◽  
Steroid Hormone ◽  
Gonadosomatic Index ◽  
Stocking Density ◽  
Sex Reversal ◽  
The Other ◽  
Daily Weight Gain ◽  
Monopterus Albus ◽  
Rice Field Eel ◽  
Significant Difference

The effects of stocking density on various growth parameters and sex reversal in the rice field eel (Monopterus albus) were investigated by evaluating steroid hormone, the gonadosomatic index (GSI) and sex ratio. Fish (mean initial weight of 9.88 ± 0.70 g) were stocked to densities of 4, 20, 36, 52, 68, 84 or 100 fish/m2 in cages (2 m × 1 m × 1.5 m) in a pond, with five replicate cages for each density. Fish were fed for 420 days on a formulated isocaloric diet containing 40.22% crude protein and 12.86 MJ/kg. Serum E2 and T concentrations were determined by radioimmunoassays. There were no significant differences in final bodyweight (FBW) and daily weight gain (DWG) for fish in the groups containing less than 52 fish/m2, while FBW and DWG were significant lower in groups at 68 fish/m2 or more. Specific growth rate (SGR) differed significantly between the 84 and 100 fish/m2 groups compared with the 4, 20 and 36 fish/m2 groups. There was no significant difference in SGR in groups containing less than 84 fish/m2. There were no significant difference between the 4 and 20 fish/m2 groups in net yield (NY), but these groups did differ significantly from the other groups. The final condition factors of fish stocked at 84 or 100 fish/m2 were significantly lower than fish stocked at the other densities. E2 and T hormone concentrations declined as stocking density increased, while GSI values increased initially but then reduced with increasing stocking density. Female ratio and survival decreased with increasing stocking density. The proportion of male fish was significantly greater in the three highest stocking density groups. Therefore, high stocking density may promote sex change from female to male in M. albus.

scholarly journals Antimicrobial Resistance among Neonates with Bacterial Sepsis and Their Clinical Outcomes in a Tertiary Hospital in Kathmandu Valley, Nepal

2021 ◽  
Vol 6 (2) ◽  
pp. 56
Author(s):  
Bijendra Raj Raghubanshi ◽  
Karuna D. Sagili ◽  
Wai Wai Han ◽  
Henish Shakya ◽  
Priyanka Shrestha ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Neonatal Sepsis ◽  
Bacterial Culture ◽  
Bacterial Species ◽  
Antibiotic Sensitivity ◽  
College Teaching ◽  
Hospital Treatment ◽  
Resistant Bacteria ◽  
Coagulase Negative Staphylococcus ◽  
Cross Sectional

Globally, antibiotic resistance in bacteria isolated from neonatal sepsis is increasing. In this cross-sectional study conducted at a medical college teaching hospital in Nepal, we assessed the antibiotic resistance levels in bacteria cultured from neonates with sepsis and their in-hospital treatment outcomes. We extracted data of neonates with sepsis admitted for in-patient care from June 2018 to December 2019 by reviewing hospital records of the neonatal intensive care unit and microbiology department. A total of 308 neonates with sepsis were admitted of which, blood bacterial culture antibiotic sensitivity reports were available for 298 neonates. Twenty neonates (7%) had bacteriologic culture-confirmed neonatal sepsis. The most common bacterial species isolated were Staphylococcus aureus (8), followed by coagulase-negative Staphylococcus (5). Most of these bacteria were resistant to at least one first-line antibiotic used to manage neonatal sepsis. Overall, there were 7 (2%) deaths among the 308 neonates (none of them from the bacterial culture-positive group), and 53 (17%) neonates had left the hospital against medical advice (LAMA). Improving hospital procedures to isolate bacteria in neonates with sepsis, undertaking measures to prevent the spread of antibiotic-resistant bacteria, and addressing LAMA’s reasons are urgently needed.

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