SOME MOLECULAR ASPECTS OF PLANT PEROXIDASE BIOSYNTHETIC STUDIES
The early history of research on peroxidase is most capably reviewed bySaunders et al (67). The first color reaction of biological material withguaiacum as substrate was noted in 1809, but the term peroxidase was usedfor the first time nearly a century later for an enzyme isolated from horseradish.In fact, peroxidase is widely distributed in the plant kingdom and also occurs in the animal kingdom. Many of the spectral aspects of this hemoproteinand its reaction products have been described in the above-mentioned textand elsewhere (e.g. 23). Different types of peroxidases were recognized, andan ever-increasing number of functions were ascribed to them, particularly inplant physiology (31). Therefore, while the basic chemical reactions ofperoxidase (E. C . 1.1 1.1. 7) are well established, namely that of the peroxidaticcycle involving H202 and a large array of hydrogen donors (23), the questionsof how its many isozymes function (31) and how its action in differentiation iscontrolled (68) remain largely unanswered. I do not review all publications onthese topics here. A comprehensive tabulation has appeared recently (31).Instead, I offer a critical review of certain aspects of peroxidase in terms ofstructure and biosynthesis.