scholarly journals Expression of CyclinD1, CDC25B and p27 protein in gastric carcinoma And it s clinical significance

2021 ◽  
Vol 12 (11) ◽  
pp. 35-39
Author(s):  
Wang Jun
Keyword(s):  
Gastric Cancer ◽  
Gastric Carcinoma ◽  
Western Blot ◽  
Lymph Nodes ◽  
Cyclin D1 ◽  
Suppressor Gene ◽  
Biological Behavior ◽  
High Morbidity ◽  
Gastric Tissue ◽  
Lymph Nodes Metastasis

Background: Gastric cancer is a common digestive tract tumor in our China, with high morbidity and mortality. Similar with other tumors, the occurrence of gastric cancer was also a complex pathophysiological process, regulated by a variety of oncogenes and tumor suppressor gene. Aims and Objectives: This study aims to investigate the expression of cyclin D1, CDC25B, and p27 in gastric carcinoma and to study their relationship with the occurrence and development of gastric cancer. Materials and Methods: SP immunohistochemical and Western blot analysis were used to detect the expression of cyclin D1, CDC25B, and p27 in 42 cases of gastric carcinoma, 42 cases of paracancer, and 42 cases of normal gastric tissue and then their relationship with clinical and pathological factors was analyzed. Results: (1) The expression of cyclin D1 increased gradually in normal gastric tissue, paracancer, and gastric carcinoma (P>0.05). The expression of CDC25B was higher in gastric carcinoma (P<0.05) while p27 was lower (P<0.05). (2) The result of Western blot shows that the expression of cyclin D1 and CDC25B was higher in gastric carcinoma than that in normal gastric tissue while p27 lower in gastric carcinoma (P<0.05), same with immunohistochemical. (3) The expressions of cyclin D1 and CDC25B had correlation with lymph nodes metastasis, and p27 had correlation with degrees of pathological differentiation, invasion depth, and lymph nodes metastasis. (4) There was a positive relationship between the expression of cyclin D1 and CDC25B in same sample (r=−0.392, P<0.05). Conclusion: Expression of cyclin D1, CDC25B, and p27 protein can be helpful in the prediction of the biological behavior and prognosis of gastric carcinoma.

Expression of cyclin D1 and p21(WAF1/CIP1) in human gastric carcinoma and its clinicopathologic significance.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e15114-e15114
Author(s):  
Vasiliki Michalaki ◽  
Theodosios Theodosopoulos ◽  
Agathi Kondi- Pafiti ◽  
Constantine G. Gennatas
Keyword(s):  
Gastric Cancer ◽  
Cell Cycle ◽  
Gastric Carcinoma ◽  
Cyclin D1 ◽  
Gastric Epithelium ◽  
Nuclear Staining ◽  
Cell Cycle Regulators ◽  
Diffuse Type ◽  
Clinicopathological Findings ◽  
P21 Waf1

e15114 Background: The deregulation of cyclin, cyclin-dependent kinases (CDKs) and their inhibitors could have a crucial role in the development of diverse human cancers. Alterations in cell cycle regulators and subsequent deregulation of the cell cycle are frequently involved in tumorigenesis and/or tumor progression. The aim of our study was to detect the abnormal expression of cyclin D1 and p21(WAF1/CIP1)in gastric carcinoma and investigate its clinicopathologic significance. Methods: Proteins of cyclin D1 were detected by immunohistochemistry in 80 cases of advanced gastric carcinoma, and 30 cases of benign gastric diseases (chronic gastritis, atrophic gastritis, gastric metaplasia, and gastric dysplasia). From each patient formaldehyde–fixed paraffin sections were stained and examined by immunohistochemistry using monoclonal antibodies.All tumor cells with distinct nuclear staining were considered positive. Results: Sixty-five patients were male and forty five female. Normal gastric epithelium showed consistently positive immunostain for p21WAF1/CIP1. Loss of p21WAF1/CIP1 expression was noted in 65% of intestinal type adenocarcinoma and in 90% of diffuse type adenocarcinoma. Overexpression of cyclin D1 was detected in 90% of advanced gastric carcinomas. Among the various clinicopathological findings, overexpression of cyclin D1 was associated with lymph-node metastasis (P=0.003) and recurrence (P=0.044). Loss of p21WAF1/CIP1 expression was more frequent in diffuse type cancers (P=0.005) and was correlated with recurrence (P=0.002) and death (P=0.001). Conclusions: These findings suggest that overexpression of cyclin D1 is a frequent finding in gastric cancer and immunohistochemical analysis for cell cycle regulators, might be a useful prognostic indicator in gastric cancer.

The prognostic VALUE of Ki-67 in gastric cancer.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e15172-e15172
Author(s):  
Antonio Caldeira Fradique ◽  
Ligia Bruno Da Costa ◽  
Alexandra Pupo ◽  
Luisa Quaresma ◽  
Gualdino Silva ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Carcinoma ◽  
Lymph Nodes ◽  
Cancer Mortality ◽  
Prognostic Value ◽  
Prognostic Indicator ◽  
Nutrition Status ◽  
Depth Of Invasion ◽  
Tumor Type ◽  
Ki 67

e15172 Background: Gastric carcinoma is the second cause of cancer mortality worldwide. In Portugal, a country with a high incidence of the disease, it is also the second leading cause of cancer mortality. The gravity of this situation and the advanced stage of disease at presentation warrants a search of a new prognostic indicator. We studied in patients (pts) with gastric cancer, both diffuse and intestinal types, several possible prognostic indicators, such as: weight, nutrition status, Ki-67, p53, Cerb2, and cadherin E. The results showed that Ki-67, an indicator of cell proliferation, may play an important role as a prognostic indicator because it is easily assessed by immunohistochemistry, is observed in both intestinal and in diffuse tumor types, and is related with tumor size, depth of invasion and the pattern of lymphatic spread. Methods: A total of 50 pts with gastric carcinoma (25 diffuse and 25 intestinal types) undergoing radical gastrectomy with extended lymphadenectomy (D2 and a few D3 types ) were prospectively studied by immunohistochemistry for the determination of Ki-67 in biopsied tissue and gastrectomy specimens. A relationship between the Ki-67 and various other prognostic factors, like histological tumor type, tumor location, number of involved lymph nodes, depth of invasion, sex and stage was established. Results: A total of 50 tumor specimens and an equal number of biopsies were evaluated for Ki-67 overexpression. Ten tumors (20%) presented a Ki-67 < 50%, 13 tumors (26%) showed a Ki-67 >50% < 74%, and 27 tumors (54%) had a Ki-67 > 75%. It was found that tumors with a low Ki-67 expression have a more proximal location, the histological type was either mixed or diffuse, tend to invade superficially the gastric wall, they don’t have metastases in distal lymph nodes, and are diagnosed in early stages of the disease. Conclusions: In this study, the Ki-67 overexpression showed prognostic value in both diffuse and intestinal type gastric cancer patients.

scholarly journals A Case of Long-Term Survival after Splenectomy for Solitary Splenic Metastasis from Gastric Cancer

2020 ◽  
Author(s):  
Ayato Obana ◽  
Nobuo Komatsu ◽  
Kazuma Aiba ◽  
Shinya Nakanishi ◽  
Masakazu Abe ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Carcinoma ◽  
Lymph Nodes ◽  
Recurrence Free Survival ◽  
Splenic Metastasis ◽  
Free Survival ◽  
Solitary Metastasis ◽  
Serum Carcinoembryonic Antigen ◽  
Splenic Tumor

Abstract Background: Very rarely does a splenic solitary metastasis arise from a gastric carcinoma because splenic metastasis is usually seen in association with widespread visceral metastasis. Splenectomy is considered to be a curative treatment; however, long-term prognosis after splenectomy has scarcely been reported. We report a case of a metachronous and solitary metastasis to the spleen from gastric cancer in which the patient achieved 5-year recurrence-free survival after splenectomy.Case Presentation: An 84-year-old man underwent an open total gastrectomy involving D1+ lymph nodes dissection for gastric cancer located in the cardia (pT3N1M0, pStage IIB). Eighteen months later, a 2-cm solitary hypodense lesion was detected in the spleen by computed tomography (CT). Twenty-three months later, the serum carcinoembryonic antigen (CEA) value elevated to 19.9 ng/ml, and abdominal CT revealed an increase in tumor size to 5 cm. Positron-emission tomography (PET)-CT revealed intense 18F-2-deoxy-2-fluoro-glucose (FDG) uptake in the spleen without the involvement of other organs and lymph nodes. We diagnosed him with solitary splenic metastasis from gastric cancer and performed a splenectomy 26 months after the first surgery. Histological examination revealed that the splenic tumor was a moderately differentiated adenocarcinoma, which was very similar to the primary gastric tumor; the lesion was diagnosed as a metastatic tumor from the previous gastric carcinoma. The patient remains healthy to date without recurrence, 5 years after the splenectomy. Conclusion: We experienced a case of a solitary splenic metastasis from gastric cancer in which 5-year recurrence-free survival was achieved after splenectomy. To determine the surgical indication in patients with splenic metastasis, it is important to differentiate between a solitary lesion or multiple metastasis. Especially, occult metastasis should be excluded by means of several months of follow-up with imaging tests and systemic FDG-PET surveys before splenectomy.

scholarly journals MicroRNA-200b reduced ZEB1 to inhibit cell proliferation and migration in human gastric cancer

2019 ◽  
Author(s):  
He Chen ◽  
Pengcheng Liu ◽  
Lei Wang ◽  
Yanxia Yu ◽  
Yan Zhao ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Suppressor ◽  
Cyclin D1 ◽  
Luciferase Reporter ◽  
High Morbidity ◽  
Human Gastric Cancer ◽  
Luciferase Reporter Gene ◽  
And Migration ◽  
Tgf Β1 ◽  
Proliferation And Migration

Abstract BACKGROUND Gastric cancer (GC) is one of the most common malignant cancers, with high morbidity and mortality rates worldwide. The present study was to explore whether miR-200b is a tumor suppressor in GC and to unveil the potential mechanisms.METHODS Levels of c-Myc, Cyclin D1, MMP-3 and MMP-9 expression were detected respectively by q-PCR and Western blot assay. BrdU proliferation assay, Cell cycle analysis and Wound-healing were used to study the role of miR-200b with inhibitor, mimics or ZEB1-RNAi in TGF-β1-treated SGC-7901/DDP cells.RESULTS Compared with the paracancerous tissues, miR-200b was decreased in GC patients and SGC-7901/DDP cells. Lower level of miR-200b induced by its inhibitor promoted TGF-β1-treated SGC-7901/DDP cells proliferation and migration, and increased the levels of c-Myc, Cyclin D1, MMP-3, and MMP-9. Interestingly, miR-200b mimics and ZEB1-RNAi were able to reduce the proliferation and migration of TGF-β1-induced SGC-7901/DDP cells as well as their levels of c-Myc, Cyclin D1, MMP-3 and MMP-9. In addition, ZEB1 was indeed the potential target of miR-200b identified by dual luciferase reporter gene assay.CONCLUSION Taken together, our findings suggest that miR-200b be likely to play an important role in activating TGF-β1-induced SGC-7901/DDP cells and perform as a tumor suppressor by targeting ZEB1 in GC.

scholarly journals STUDIES ON LYMPH NODES METASTASIS OF SUBMUCOSAL GASTRIC CANCER-Aspects of tumor characteristics and host responses-

1983 ◽  
Vol 44 (1) ◽  
pp. 8-13
Author(s):  
Kimio IGUCHI ◽  
Tsuguo TANAKA ◽  
Hideaki KURIOKA ◽  
Kazuyo NAITO ◽  
Shinhachiro NOOMI ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Nodes ◽  
Host Responses ◽  
Tumor Characteristics ◽  
Lymph Nodes Metastasis ◽  
Submucosal Gastric Cancer

scholarly journals Clinicopathological Review of Resectable Gastric Cancer at a Tertiary Level Teaching Hospital of Nepal

2020 ◽  
Vol 42 (1) ◽  
pp. 74
Author(s):  
Prasan Kansakar ◽  
Pradeep Vaidya
Keyword(s):  
Gastric Cancer ◽  
Gastric Carcinoma ◽  
Lymph Nodes ◽  
Teaching Hospital ◽  
Curative Resection ◽  
Locally Advanced ◽  
Subtotal Gastrectomy ◽  
Distal Margin ◽  
Tumor Type ◽  
Borrmann Type

Introduction According to available data, gastric carcinoma is the most common malignancy arising from the gastrointestinal tract in Nepal. Our objective was to analyze the pathological features of gastric carcinoma in patients undergoing curative resection. MethodsRetrospective analysis of histopathological reports of patients who underwent curative surgery for gastric cancer was performed at Department of Surgery, Tribhuvan University Teaching Hospital for a period of two years. Demographic characteristics, symptoms, site of tumor, type of surgery performed, macroscopic and microscopic characteristics including number of harvested lymph nodes, number of positive lymph nodes and tumor stage were analyzed. ResultsA total of 36 patients underwent curative resection. Mean age of the patients was 61.6 years with range of 21-82 years. Male:female ratio was 7:4. Pain abdomen was the predominant presenting symptom. Vomiting, hematemesis and malena were the other frequent symptoms. Endoscopy Borrmann type II tumor was seen in 27 (75%) of patients. Most patients underwent subtotal gastrectomy. In 25 patients, tumor was located in antrum. Lymphovascular and perineural invasion was seen in 21 and 20 patients respectively. Sixteen patients had poorly differentiated tumors. All but two patients had microscopic negative distal margin whereas proximal margin was negative in all patients. A mean of 15 lymph nodes were harvested. Twenty three patients had node positive disease. Pathological staging revealed Stage IA in 3 patients, Stage IB in in 5 patients , stage IIA in 4 patients stage IIB in 6 patients, stage IIIA in 6 patients, stage IIIB in 8 patients and IIIC in 4 patients. ConclusionGastric carcinoma was common in seventh and eighth decade of life with male preponderance. Most of the patients presented with locally advanced gastric cancer. Tumors located in antrum requiring subtotal gastrectomy was the predominant finding.

scholarly journals A Case of Mucosal Gastric Cancer with pN3a Lymph Nodes Metastasis

2013 ◽  
Vol 74 (6) ◽  
pp. 1506-1510
Author(s):  
Keiichi YAMADA ◽  
Masayoshi YAMAMOTO ◽  
Youichi OKUDA ◽  
Youhei MORITA ◽  
Kentaro NAGAI ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Lymph Nodes ◽  
Lymph Nodes Metastasis ◽  
Mucosal Gastric Cancer
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