scholarly journals Characterisation and Isolation of Candida Species from ICU Patients in Nobel Medical College Teaching Hospital, Biratnagar

2016 ◽  
Vol 5 (1) ◽  
pp. 61-65
Author(s):  
Bigu Kumar Chaudhari ◽  
Ganesh K. Singh ◽  
Kamal Prasad Parajuli ◽  
Kewal Shrestha ◽  
Dharmendra Shah
Keyword(s):  
Candida Albicans ◽  
Teaching Hospital ◽  
Candida Species ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antifungal Drugs ◽  
Clinical Samples ◽  
Care Hospital ◽  
Sensitivity Testing ◽  
Medical College

Background Fungi have emerged as major causes of human diseases. Intensive Care Units (ICU), harbor almost all the risk factors for opportunistic fungal infections. Among these, Candida infections are very common with recent trends being rise in the non-Candida albicans (NCA) species along with an increase in resistance of these species to antifungal drugs. Increament in invasive Candidasis during last three decades have been reported in several studies, among all Candida species the Candida albicans is considered as the most common infectious agent the other non-albicans like C. Tropicalis, C. glabrata, C. parapsilosis C. krusei, and C. dubliniensis were raised as infectious agents. The aim of current study is to characterize the candida species from the clinical specimens of patients admitted in the ICU of Tertiary Care hospital in Eastren Nepal and to perform their antifungal susceptibility.Material and Methods The study was carried out in the tertiary care hospital Nobel Medical College and Teaching Hospital Biratnagar Nepal over a period of 6 monthsbetween September 2015 to February 2016. The following techniques were employed to characterize the isolates in the study – Gram’s stain, culture on Sabouraud’s Dextrose Agar, Germ Tube test, morphology in Cornmeal Agar and chromogenic agar media, sugar fermentation and sugar assimilation tests, and the results were interpreted by using standard protocols.Results Out of 50 candida isolates from different clinical samples including 37 blood samples, 9 urines and 4 Endo Tracheal (ET) tube, the most common species was C.albicans (44%) followed by C.tropicalis (26%), C.Glabrata (18%), C.Parapsilosis (08%), C.Krusei (02%), and C.Dubliniensis(02%).Conclusion The purpose of the study is to show the value of species isolation, identification and antimicrobial sensitivity testing of the ICU & NICUs patients.Journal of Nobel Medical CollegeVolume 5, Number 1, Issue 8, January-July 2016, 61-65

scholarly journals Prevalence of Candidiasis Infection & Antifungal Susceptibility Pattern at Tertiary Care Hospital, Jaipur

2020 ◽  
pp. 8-13
Author(s):  
Falguni Sharma ◽  
Ved Prakash Mamoria ◽  
Ekadashi Rajni Sabharwal ◽  
Richa Sharma
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Fungal Pathogens ◽  
Antifungal Susceptibility ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Medical College ◽  
Number Of Patients ◽  
Gandhi Medical College

Background: Candida species are among the most common fungal pathogens. They are ubiquitous yeasts found on plants and form the microbiota of the alimentary tract of mammals and the mucocutaneous membranes in humans. Methods: A total of 90 clinical samples were collected from Mahatma Gandhi Medical College & Hospital, Jaipur over a period of one year. Primary identification is done by the direct smear examination by Gram’s staining and KOH mount and then further sub-cultured on SDA media.  Results: Out of 90 clinical specimens collected, most common form of Candidiasis seen was Candiduria type followed by blood cultures, swab, Endotracheal tube, sputum and Bronchialveolar lavage. The maximum number of patients were found in the age group of 21-30 years, followed by 51-60 years. Out of 90 patients, 48 (53.3%) were males and 42 (46.6%) were females. C. tropicalis (44.4%) was the predominant species followed by C. albicans and other species of Candida. Among 90 samples, 32% were Candida albicans and remaining 68% were Non albicans Candida species. Conclusion: The study found that C. tropicalis was sensitive against Micafungin & Caspofungin, showing a 100% sensitivity. Candida albicans showed 100% sensitivity against Flucytosine.

scholarly journals PREVALENCE AND ANTIBIOTIC SUSCEPTIBILITY OF ACINETOBACTERIN A TERTIARY CARE HOSPITAL IN CHENNAI, INDIA

2021 ◽  
pp. 35-40
Author(s):  
ANGELINE ANJALI A. ◽  
ABIRAMASUNDARI V. K.
Keyword(s):  
Antibiotic Susceptibility ◽  
Tertiary Care Hospital ◽  
Therapeutic Option ◽  
Tertiary Care ◽  
Fourth Generation ◽  
Clinical Samples ◽  
Care Hospital ◽  
Acinetobacter Species ◽  
Medical College ◽  
Acinetobacter Infection

Objective: The present study is to determine the prevalence and antibiotic susceptibility of Acinetobacter species in samples collected from patients in tertiary care hospital in Chennai. Methods: A total of17,827patient’s clinical samples were collected from various wards and ICUs of Saveetha Medical College and Hospital, Chennai, Tamilnadu over a period of 7 mo [between January 2020 and July 2020]. All samples were tested in the microbiology lab of Saveetha Medical College and Hospital using standard operating procedures. Results: Out of 17,827 samples, 2,816 were culture positive. 122 of the isolates tested positive for Acinetobacter spp.and 81.1% of the isolates belonged to Acinetobacterbaumannii. Most of the infection occurred in the age group of 21-40 y and predominantly in female patients (female, male ratio 1.9:1).General wards contributed to 54.9% of the Acinetobacter infection, followed by ICU(27%) and OPD(18%). Maximum isolates were recovered from urine(34.4%) and endotracheal secretions(29.5%).60.7% of the Acinetobacterspp were multidrug-resistant(MDR)i.e. resistant to more than 3 antibiotic group.In our study, most Acinetobactersppwere resistant to penicillin(46-100%), third and fourth generation cephalosporin (36-61.5%), carbapenems (34.4-82.8%)and quinolones(39.3-46.7%). None of the isolates were resistant to colistin. 93.4% ofisolates were sensitive to tigecycline and 87.7% sensitive to amikacin. Conclusion: Our study observed a high incidence of MDR inAcinetobacterspp, which is in line with most of the research findings in recent times. Most of Acinetobacterspp were resistant to penicillin, third and fourth generation cephalosporins, quinolones, carbapenems,which is alarming as it leaves fewer options for the line of treatment. Some strains were sensitive to cefepime, ceftazidime, piperacillin-tazobactam, levofloxacin, imipenem and meropenem. Considering the increasing MDR nature of Acinetobacterspp a combination of the former along with colistin, tigecycline, amikacin(which have shown more than 85% sensitivity) would need to be studied.Also, strict measures to control the spread of Acinetobacter infection, better management of antibiotics usage and newer therapeutic option for treatment need to be looked at.

scholarly journals Prevalence of ocular disorders with seasonal variations in Tertiary Care Hospital in South Punjab.

2020 ◽  
Vol 27 (08) ◽  
pp. 1675-1679
Author(s):  
Jamila Anwar ◽  
Muhammad Luqman Ali Bahoo ◽  
Faisal Ali ◽  
Kanwal Ijaz
Keyword(s):  
Teaching Hospital ◽  
Seasonal Variations ◽  
Tertiary Care Hospital ◽  
Demographic Data ◽  
Tertiary Care ◽  
Care Hospital ◽  
Medical College ◽  
Ocular Disorders ◽  
Ocular Morbidity ◽  
Study Setting

Objectives: To observe the Prevalence of Ocular Disorders with seasonal variations in South Punjab. Study Design: Observational descriptive study. Setting: Ophthalmology Department of Shahida Islam Teaching Hospital affiliated with Shahida Islam Medical College Lodhran, a tertiary care Hospital in south Punjab. Period: January 2018 to December 2018. Material & Method: The case records of all consecutive patients seen at the outpatient department (OPD) of Shahida Islam Teaching Hospital, Lodhran from January 2018 to December 2018 were retrieved. The demographic data such as age and sex of the patients were noted. The patient data were collected from the OPD registers at the clinics and analyzed retrospectively. Results: A total of 14398 patients were examined out of which male were 46.3% and female were 53.7%. Ocular examination revealed that out of 14398 patients most common ocular morbidity is refractory errors followed by conjunctiva diseases and then lenticular disorders. Conclusion: Majority of ocular morbidity were either preventable or treatable. If detection is early in the course, prevalence of ocular morbidity can be greatly reduced.

scholarly journals Speciation of Candida using CHROMagar from various clinical specimens and their antifungal susceptibility pattern at a tertiary care hospital.

2020 ◽  
Vol 16 (2) ◽  
pp. 107-111
Author(s):  
Sanjana Raj Kumari ◽  
Neetu Adhikaree
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Tertiary Care ◽  
Epidemiological Data ◽  
Antifungal Susceptibility Testing ◽  
Antifungal Drugs ◽  
Care Hospital ◽  
Clinical Specimens

Background: Candida albicans remains the most common and are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases. But recent epidemiological data shift from C.albicans to non albicans Candida species and also increased resistance to antifungal drugs made the scenario a serious concern. Methods: A total of 156 Candida isolates from various clinical specimens received in the department of Microbiology were taken up for the study over a period of one year i.e. from March 2019 to February 2020. The Candida were grown on Sabouraud dextrose agar to be  evaluated for colony  appearance, macroscopic examination, Gram staining, germ tube, urea hydrolysis etc. The Candida isolates were speciated by using CHROMagar medium. Antifungal susceptibility testing was performed as recommended by Clinical and Laboratory Standards Institute (CLSI) M44-A document. Results: The isolation of non albicans Candida (54.5%) predominated over Candida albicans (45.5%). Non albicans Candida isolated were Candida tropcalis 40(25.6%), Candida krusei 21(13.4%), Candida glabrata 17(10.8%) and Candida dublinensis 07(4.4%) each. Candida species were all susceptible to Amphotericin B, followed by fluconazole (67.4%), miconazole (51.9%) and ketoconazole (22.5%). Conclusions: The accurate species identification of Candida is important for the treatment because not all species respond to the same treatment and also because of the increasing antifungal resistance. CHROMagar is a convenient and rapid method of identification of Candida species specially in resource limited poor settings.   Keywords: antifungal susceptibility testing; Candida albicans; CHROMagar; non albicans Candida

scholarly journals Prevalence of ESBL and MBL Producing Acinetobacter species Isolated from Various Clinical Samples in Tertiary Care Hospital in North-West Region of Rajasthan

2021 ◽  
Vol 10 (10) ◽  
pp. 524-532
Author(s):  
Sumitra Kumari Sanju Pannu ◽  
Anjli Gupta Geeta Tinna ◽  
B.P. Sharma
Keyword(s):  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Care Hospital ◽  
Sensitivity Testing ◽  
Acinetobacter Species ◽  
North West ◽  
Medical College ◽  
Vaginal Swabs

This study was conducted with an objective to find the prevalence of extended spectrum betalactamase (ESBL) and metallo betalactamase (MBL) in Acinetobacter species. It was conducted in the Department of Microbiology, Sardar Patel Medical college, Bikaner from Feb 2018 to Feb.2019. in various clinical specimens including urine, pus, blood, vaginal swabs, respiratory samples, and various body fluids were processed Acinetobacter species isolates were identified by standard protocols. Antibiotic sensitivity testing for all isolates was done using Kirby-Bauer disc diffusion method. Disc potentiation test was performed to check ESBL and MBL production in these bacteria. Maximum ESBL and MBL positive isolates of Acinetobacter species were observed among E.T. tube samples. Early detection, stringent antibiotic policies, and compliance towards infection control practices are the best defenses against this organisms.

scholarly journals Rise in antifungal resistance of Candida species from clinical samples in a tertiary care hospital, Kolkata.

2021 ◽  
Vol 39 ◽  
pp. S114
Author(s):  
C.H.A.Y.A.N.I.K.A. BANERJEE ◽  
Swagata Ganguly Bhattacharjee ◽  
Soma Sarkar ◽  
Soumodip Dutta
Keyword(s):  
Candida Species ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antifungal Resistance ◽  
Clinical Samples ◽  
Care Hospital

CHARACTERISATION AND ANTIFUNGAL SUSCEPTIBILITY PATTERNS OF CANDIDA SPECIES ISOLATED IN TERTIARY CARE HOSPITAL IN NORTH INDIA.

2021 ◽  
pp. 7-8
Author(s):  
Meenakshi Sharma ◽  
Chaudhary BL
Keyword(s):  
Candida Albicans ◽  
Candida Species ◽  
Tertiary Care Hospital ◽  
Antifungal Susceptibility ◽  
Tertiary Care ◽  
Diffusion Method ◽  
Individual Species ◽  
Care Hospital ◽  
Clinical Specimens ◽  
Susceptibility Patterns

Candida is usually considered a commensal with the potential to cause opportunistic infections ranging from supercial mucosal infections to lifethreatening invasive infections. This study was aimed to determine antifungal susceptibility patterns of Candida species isolated from various clinical specimens from a tertiary care hospital. Isolation and identic Material and Methods: ation of Candida species were done by morphological examination, pseudohyphae, and chlamydospore production on cornmeal agar, germ tube test, and HiCrome Candida differential agar. The antifungal susceptibility of Candida strains was performed by the disk diffusion method as per the CLSI M44-A2 protocol. A Result: total of 96 Candida species were isolated from various clinical specimens. The isolation rate was more in the older age group and female patients(52%). Most frequently isolated from urine 41.67% followed by sputum 19.79%, Blood 16.67%, and least from vaginal swab 2.08%. Four species of Candida were isolated among them Candida albicans was most common 59.37% followed by Candida prapsilosis and Candida krusei 15.62%, and Candida tropicalis 9.37%. In antifungal susceptibility testing, Itraconazole was the most sensitive drug and Fluconazole was the least sensitive one with only 58.33% susceptibility among individual species. Concussion: Urinary tract infection was common due to Candida albicans, and Itraconazole was the most susceptible drug.

scholarly journals Rapid identification and susceptibility pattern of various Candida isolates from different clinical specimens in a tertiary care hospital in Western Uttar Pradesh

2017 ◽  
Vol 5 (8) ◽  
pp. 3466
Author(s):  
Rajani Singh ◽  
Rajesh K. Verma ◽  
Sunita Kumari ◽  
Amit Singh ◽  
Dharmendra P. Singh
Keyword(s):  
Candida Species ◽  
Antifungal Susceptibility ◽  
Tertiary Care ◽  
Underlying Disease ◽  
Vaginal Swab ◽  
The Body ◽  
Clinical Samples ◽  
Care Hospital ◽  
Human Beings ◽  
Sensitivity Testing

Background: Candida species are component of normal flora of human beings. Candidiasis is the commonest fungal disease affecting mucosa, skin, nails and internal organs. A variety of predisposing factors are known to cause candidiasis either by altering balance of normal microbial flora of the body or by lowering the host defence.Methods: A total of 90 specimens submitted in the department of microbiology were included in this study.  Identification of Candida species as well as antifungal sensitivity testing was performed with Vitek®2 compact (Biomerieux France) using Vitek2 cards for identification of yeast and yeast like organisms (ID-YST cards). Antifungal susceptibility testing was performed using Vitek2 fungal susceptibility card (AST YS01) kits respectively.Results: The distribution of the clinical samples were urine 53 (58.9%), sputum 14 (15.5%), blood 10 (11.1%), nail 6 (6.7%) and high vaginal swab 7 (7.8%). Among 90 clinical isolates, species obtained were C. tropicalis 53 (59%), C. albicans 23 (25.5%), C. glabrata 6 (6.7%), C. parapsilosis 4 (4.4%), C. krusei 2 (2.2%), C. pelliculosa 1 (1.1%), C. famata 1 (1.1%).Conclusions: Infections caused by non-candida albicans species have increased. Identification of Candida species and their antifungal susceptibility are important for the treatment of hospitalized patients with serious underlying disease.

Sign in / Sign up

Export Citation Format

Share Document