scholarly journals Dengue Virus Detection by Serological and Molecular Method in Different Hospitals of Nepal

2013 ◽  
Vol 11 (2) ◽  
pp. 24-28
Author(s):  
Yogendra Shah ◽  
Govind Prasad Gupta ◽  
Kishor Pandey ◽  
Sher Bahadur Pun ◽  
Krishna Prasad Pant ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Age Groups ◽  
Virus Detection ◽  
Public Health Problem ◽  
Viral Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Rt Pcr ◽  
Serum Samples ◽  
Disease Hospital

Introduction: Dengue is an emerging mosquito-borne viral disease in the world and is the serious public health problem of Nepal. Methods: This study was designed to determine sero-epidemiology of dengue virus infection during the period (June-Nov) of 2010 among suspected patients with fever visiting Koshi Zonal Hospital (KZH), Biratnagar, Narayani sub-regional Hospital (NSH), Birgunj, Sukraraj Tropical and Infectious Disease Hospital (STIDH), Kathmandu and Dhading District Hospital (DDH), Dhadingbeshi. The sero-prevalence of anti-dengue IgM antibody was determined by enzyme linked immunosorbent assay (ELISA). Results: Among 271 serum samples tested, the anti-dengue IgM positivity was 14.4%. Sero-positivity in male was 10.7% of total and that in female was 3.7%.  Among different age groups, the highest positive cases 11.8% were from age group 15-50 years and found least among the age group above 50 years 0.4%. Out of 4 different hospitals, the highest positive positive cases from STIDH with 9.2% and the least positive cases were from DDH (0.4%). RT-PCR showed 4.7% positivity of 21 samples tested. Conclusions: Enzyme immunoassay and RT-PCR serological marker can be used to diagnose the acute patients of dengue during outbreaks.Medical Journal of Shree Birendra Hospital; July-December 2012/vol.11/Issue2/24-27 DOI: http://dx.doi.org/10.3126/mjsbh.v11i2.7905 

scholarly journals Serological and Molecular Study of Dengue Viruses in Different Hospitals of Nepal

2013 ◽  
Vol 2 (1) ◽  
pp. 20-25 ◽  
Author(s):  
GP Gupta ◽  
Y Shah ◽  
A Poudel ◽  
R Pun ◽  
KP Pant ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Public Health Problem ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Serum Samples ◽  
Important Public Health Problem ◽  
Monsoon Period ◽  
Occupation Group ◽  
Hilly Region ◽  
Acute Patients

Background: Dengue Virus (DV) is an emerging mosquito borne viral disease and important public health problem in low land of Terai region which is also expanding to hilly region. Methods: This study was designed to estimate sero-prevalence of dengue virus infection in the post monsoon period (Jun-Dec) of 2010 in Nepalese patients with fever visiting hospitals of Birganj, Damouli, Biratnagar and Dhading Besi. Serum samples were collected from 280 patients visiting hospitals with history of fever & clinically suspected dengue fever. The sero-prevalence of dengue virus specific IgM was determined by enzyme linked immunosorbent assay (ELISA) kit (SD, Korea) Results: The anti-dengue IgM positivity was found to be 8.2%. The positive dengue cases were higher in male (10.5%) as compared to female (6.5%). Among different age groups, the highest positive cases (11.5 %) were from age group below 15 years followed by above 50 years age group with 8.5%. Out of 4 hospitals, the highest positive cases were in Tanahu District Hospital, Damouli (23.8%) followed by Koshi Zonal Hospital, Biratnagar (12.5%). Age and gender were found to be independent predictors. The highest numbers of dengue positive cases were in occupation group business (13.3%) followed by agriculture (11.5%). Conclusion: The dengue positivity was estimated in acute patients from different hospitals of Nepal by enzyme immunoassay and reverse transcriptase polymerase chain reaction. Therefore, the serological marker can be used to diagnose the acute patients of dengue during outbreaks. Nepal Journal of Medical Sciences | Volume 02 | Number 01 | Jan-Jun 2013 | Page 20-25 DOI: http://dx.doi.org/10.3126/njms.v2i1.7646

scholarly journals Performance Evaluation of Commercial Dengue Diagnostic Tests for Early Detection of Dengue in Clinical Samples

2017 ◽  
Vol 2017 ◽  
pp. 1-4 ◽  
Author(s):  
Tuan Nur Akmalina Mat Jusoh ◽  
Rafidah Hanim Shueb
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Dengue Infection ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Serum Samples ◽  
Kappa Agreement

The shattering rise in dengue virus infections globally has created a need for an accurate and validated rapid diagnostic test for this virus. Rapid diagnostic test (RDT) and reverse transcription-polymerase chain reaction (RT-PCR) diagnostic detection are useful tools for diagnosis of early dengue infection. We prospectively evaluated the diagnostic performance of nonstructural 1 (NS1) RDT and real-time RT-PCR diagnostic kits in 86 patient serum samples. Thirty-six samples were positive for dengue NS1 antigen while the remaining 50 were negative when tested with enzyme-linked immunosorbent assay (ELISA). Commercially available RDTs for NS1 detection, RTK ProDetect™, and SD Bioline showed high sensitivity of 94% and 89%, respectively, compared with ELISA. GenoAmp® Trioplex Real-Time RT-PCR and RealStar® Dengue RT-PCR tests presented a comparable kappa agreement with 0.722. The result obtained from GenoAmp® Real-Time RT-PCR Dengue test showed that 14 samples harbored dengue virus type 1 (DENV-1), 8 samples harbored DENV-2, 2 samples harbored DENV-3, and 1 sample harbored DENV-4. 1 sample had a double infection with DENV-1 and DENV-2. The NS1 RDTs and real-time RT-PCR tests were found to be a useful diagnostic for early and rapid diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.

scholarly journals Seroprevalence and Clinical Presentation of Chikungunya Virus Infection among Febrile Outpatients Seeking Health Care in Mzuzu City, Malawi

2021 ◽  
Author(s):  
Flywell Kawonga ◽  
Gerald Misinzo ◽  
Dylo Pemba ◽  
Leonard Mboera ◽  
Isaac Thom Shawa
Keyword(s):  
Chikungunya Virus ◽  
Clinical Presentation ◽  
Age Groups ◽  
Clinical Signs ◽  
Signs And Symptoms ◽  
Viral Disease ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Antibodies

Chikungunya is a mosquito-borne viral disease caused by Chikungunya virus (CHIKV. We conducted this study determine the seroprevalence and clinical presentation of Chikungunya infection among outpatients seeking healthcare in Mzuzu City, Malawi. Blood samples were collected from malaria negative and non-septic febrile outpatients with fevers ≥38 °C, for not more than 5 days. The enzyme- linked immunosorbent assay (ELISA) test was used to detect anti-CHIKV IgM antibodies and its results were used to determine seroprevalence of Chikungunya. A total of 119 serum samples were tested, of these, 73 (61.3%) tested positive for anti-CHIKV IgM antibodies by ELISA. Laboratory requisition forms were used to capture demographic information such as age, sex, clinical signs and symptoms presented by the enrolled patients. Age groups of 1-9, 10- 19, 20- 29, 30- 39, 40- 49, and ≥50 years had 17.8% (n= 13), 12.3 %,( n=9), 15.1%) (n=11), 19.2%; (n=14), 17.8% (n=13) and 17.8% (n=13) proportion of seroprevalence respectively. Most of the CHIKV infected individuals presented with fever (52.05%), joint pain (45.21%) and abdominal pain (42.67%). The presence of anti- CHIKV IgM antibodies suggest the presence of recent CHIKV infection and therefore accurate laboratory assays are highly recommended for CHIKV diagnosis and appropriate management of febrile patients.

scholarly journals Seroepidemiology of Human Group C Rotavirus in Japan Based on a Blocking Enzyme-Linked Immunosorbent Assay

2001 ◽  
Vol 8 (1) ◽  
pp. 161-165 ◽  
Author(s):  
Mitsutaka Kuzuya ◽  
Ritsushi Fujii ◽  
Masako Hamano ◽  
Ritsuko Ohata ◽  
Hajime Ogura ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Age Groups ◽  
Neutralization Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Human Group ◽  
Serum Samples ◽  
Group C Rotavirus ◽  
Group A ◽  
Okayama Prefecture

ABSTRACT A novel blocking enzyme-linked immunosorbent assay (BL-ELISA) was developed for detection of antibodies to human group C rotavirus (CHRV). The specificity of the BL-ELISA was confirmed by using animal sera hyperimmunized to group A and group C rotaviruses and paired sera from five patients with acute CHRV gastroenteritis. Furthermore, there was concordance between the BL-ELISA and a neutralization assay for CHRV in 226 (95%) of 238 samples. By using the BL-ELISA, we determined the seroprevalence of CHRV in 704 serum samples obtained from nine different age groups of inhabitants of Okayama Prefecture, Japan, in 1992, 1994, and 1996. As a result, 211 sera (30%) were found to be positive for CHRV antibodies. The seroprevalence gradually increased with age and reached 52.7% in the oldest individuals. A further analysis of the youngest age group suggested that CHRVs predominantly prevail in persons older than 3 years of age in Japan. When comparing the three sampling years, a larger percentage of antibody-positive sera was detected in 1994 than in either 1992 or 1996 in individuals between 6 and 15 years of age, reflecting the occurrence of a CHRV outbreak among children during the winter of 1992 to 1993 that was previously documented. These results indicate that CHRV infections may occur more frequently in spite of the relatively low detection rate of the virus.

scholarly journals The Seroprevalence of Dengue Virus Infection and Its Association With Iron (Fe) Level in Pregnant Women in Guangzhou, China

2021 ◽  
Vol 8 ◽  
Author(s):  
Jiong Wang ◽  
Jiufeng Sun ◽  
Limei Sun ◽  
Yufeng Ye ◽  
Hanwei Chen ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Pregnant Women ◽  
Inductively Coupled Plasma ◽  
Denv Infection ◽  
Viral Disease ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Air Conditioner ◽  
Serum Samples ◽  
Igg Antibody

Dengue fever is regarded as the most prevalent mosquito-borne viral disease in humans. However, information of dengue virus (DENV) infection in pregnant women and the influence factors remain unclear. In this study, we extracted information of 2,076 pregnant women from the Prenatal Environment and Offspring Health (PEOH) birth cohort conducted since 2016 in Guangzhou, China. Peripheral blood and clean midstream urine samples of participants were collected during their hospitalization for childbirth. Indirect enzyme-linked immunosorbent assay (ELISA) was used to detect immunoglobulin G (IgG) antibodies of DENV in serum samples, and inductively coupled plasma mass spectrometry (ICP-MS) was applied to determine the Fe concentrations in the urine samples, which were then adjusted for by urine creatinine and transformed by natural logarithm (ln-Fe). The seroprevalence of DENV IgG antibody in all included participants was 2.22% (46/2,076). We observed higher seroprevalence of IgG antibody in women aged ≥35 years (2.9%), education ≤ 12 years (2.5%), yearly income per capita <100,000 yuan (2.4%), no use of air-conditioner (2.4%), no use of mosquito coils (2.3%), and no exercise during pregnancy (4.1%). A U-shaped relationship was found between ln-Fe concentration and the risk of positive IgG antibody. Compared with women with ln-Fe concentration of 2.0–2.9 μg/g creatinine, slightly higher risks of positive IgG antibody were found among women with ≤2.0 (RR = 4.16, 95% CI: 0.78, 19.91), 3.0–3.9 (RR = 1.93, 95% CI: 0.65, 7.08), 4.0–4.9 (RR = 2.19, 95% CI: 0.65, 8.51), and ≥5.0 μg/g creatinine of ln-Fe (RR = 2.42, 95% CI: 0.46, 11.33). Our findings suggested that the seroprevalence of dengue IgG antibody in pregnant women was comparable to the general population in Guangzhou, China. The risk of DENV infection may be associated with maternal demographic characteristics and behaviors. Both maternal low and high Fe concentrations may be positively associated with the risk of DENV infection.

scholarly journals Use of Particle Agglutination in the Diagnosis of Dengue Virus Infection in Western Nepal

2012 ◽  
Vol 12 ◽  
pp. 286-289 ◽  
Author(s):  
Ramesh Pun ◽  
Krishna P Pant ◽  
Yogendra Shah ◽  
Shardulendra P Sherchand ◽  
Basu D Pandey
Keyword(s):  
Dengue Virus ◽  
Statistical Significance ◽  
Viral Disease ◽  
Cross Sectional Study ◽  
Western Region ◽  
Age Group ◽  
Serum Samples ◽  
Cross Sectional ◽  
Demographic Distribution ◽  
Particle Agglutination

Dengue is an emerging vector borne viral disease in Nepal. The study was conducted to detect dengue virus specific IgM and to analyze demographic distribution of positive cases. This is a cross-sectional study carried out in western region of Nepal from August-October 2007. A total of 116 serum samples were collected from suspected dengue virus infected patients visiting hospitals and investigated by particle agglutination. Out of 116 sera, 12 (10.34%) had a positive dengue virus specific IgM. The positivity in age group below 15 years old was 23.08%, 7.32% in 15-50 years age group and 14.29% above 50 years age group. The ratio of positivity in male and female was 1:1. However, there were no statistical significance in the positivity with age (P=0.27) and sex (P=0.91). Thus, dengue has been established in western region of Nepal and diagnosis of the disease can be done by particle agglutination assay. The particle agglutination should be suggested for the diagnosis of dengue in all health centers of Nepal. Awareness programmes on prevention of dengue and surveillance of the disease should be conducted to reduce morbidity and mortality during possible outbreak of it.DOI: http://dx.doi.org/10.3126/njst.v12i0.6514 Nepal Journal of Science and Technology 12 (2011) 286-289 

scholarly journals Co-Circulation of Dengue Serotypes in Coastal Andhra Pradesh, India - A Descriptive Study

2020 ◽  
Vol 9 (40) ◽  
pp. 2965-2969
Author(s):  
Suryamani Chintapalli ◽  
Apparao Peddepalli ◽  
Sivajyothi Pilli ◽  
Monika Deepthi Pilli ◽  
Kanaka Mahalakshmi Yandra
Keyword(s):  
Dengue Virus ◽  
Andhra Pradesh ◽  
Febrile Illness ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Serum Samples ◽  
Medical College ◽  
Igm Antibodies ◽  
Dengue Outbreaks ◽  
Ns1 Antigen

BACKGROUND Dengue is an acute febrile illness caused by mosquito-borne dengue viruses (DENV S) consisting of four serotypes (DENV 1 - 4) from flaviviridae family, genus flavivirus. These four are antigenically related serotypes designated as DEN V - 1, DEN V - 2, DEN V - 3 and DEN V – 4. In this context, the present study focuses on the circulating serotypes of dengue in coastal Andhra Pradesh. METHODS Study was done at Andhra Medical College, Visakhapatnam, teaching hospital in Andhra Pradesh. Acute phase dengue serum samples were collected and tested for NS1 antigen and antihuman IgM antibodies by enzyme linked –immunosorbent assay (ELISA). NS1 positive samples were further serotyped by reverse transcriptase real time polymerase chain reaction (R RT - PCR). RESULTS A total of 796 serum samples were included in the study. 300 (37.7 % ) samples were positive for NS1 and IgM antibodies. 192 NS1 antigen positive samples were further processed for serotyping by r RT PCR. Among these samples 72 were negative by r RT PCR. DENV-2 (41 %) was the predominant serotype followed by DENV-4 (37 %), DENV-3 (12 %) and DENV-1 (10 %) in the descending order. CONCLUSIONS All the four dengue serotypes are in co-circulation. Among all the four types, DENV-2 was predominant, followed by DENV-4. By knowing the predominant serotype in circulation, we can forecast dengue outbreaks and take necessary measures like control of vectors. KEY WORDS Andhra Pradesh, Dengue Virus, Dengue Virus - 2, Dengue Virus - 4, Outbreak, Serotypes

scholarly journals Generation and Characterization of Monoclonal Antibodies against Dengue Virus Type 1 for Epitope Mapping and Serological Detection by Epitope-Based Peptide Antigens

2007 ◽  
Vol 14 (4) ◽  
pp. 404-411 ◽  
Author(s):  
Yun-Ching Chen ◽  
Hsien-Neng Huang ◽  
Chin-Tarng Lin ◽  
Yi-Fang Chen ◽  
Chwan-Chuen King ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Dengue Virus ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Mouse Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Random Peptide Library ◽  
Peptide Antigens ◽  
Neutralizing Epitopes

ABSTRACT Dengue virus (DEN), the pathogen behind dengue hemorrhagic fever, remains a public health problem in Asia and South America. In this study, monoclonal antibodies (MAbs) against DEN serotype 1 (DEN-1) were generated by fusing NSI/1-Ag4-1 mouse myeloma cells with lymphocytes from BALB/c mice immunized with DEN-1. Twelve MAbs were found to react specifically to the DENs by enzyme-linked immunosorbent assay, immunofluorescence analysis, and immunoblotting analysis. Five MAbs, namely, DA4-7, DA6-7, DA9-5, DA10-2, and DA11-13, were found to react with envelope proteins of DEN-1. Two serotype-specific MAbs of DEN-1, DA6-7 and DA11-13, were further shown to neutralize DEN-1 infection by a plaque reduction neutralization test. The neutralizing epitopes of these MAbs were further identified from a random peptide library displayed on phage. Immunopositive phage clones reacted specifically with these MAbs and did not react with normal mouse serum. Epitope-based peptide antigens were proved able to detect antibodies in serum samples collected from DEN-1-infected patients but not in those taken from DEN-2-infected patients or healthy controls. We believe that these MAbs and neutralizing epitopes will provide information that will lead to the development of DEN-1 serotype-specific diagnostic reagents and vaccines.

scholarly journals Seroprevalence of Hepatitis E Virus (HEV) Infection Among Patients Attending BSMMU, Dhaka

2016 ◽  
Vol 1 (2) ◽  
pp. 52-55
Author(s):  
Ayesha Begum ◽  
Shahina Tabassum ◽  
Md. Nazrul Islam ◽  
Ms Afzalunnessa
Keyword(s):  
Urban Areas ◽  
Hepatitis E Virus ◽  
Public Health Problem ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Age Group ◽  
Serum Samples ◽  
Rural And Urban Areas ◽  
Rural And Urban ◽  
Study Population

The present study was done to determine the seroprevalence of the infection by Hepatitis E virus (HEV) among patients attending Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka. Serum samples from 408 subjects, aged between 0-69 years, attending BSMMU from different rural and urban areas of Bangladesh were tested for HEV IgG by enzyme linked immunosorbent assay (ELISA). Anti-HEV IgG was found among 20.6% of the study population. A higher rate of seropositivity (26.1%) was found among patients attending from urban than those from rural (15.1%) areas which is statistically significant (P < 0.01). A gradual rise in prevalence of anti-HEV IgG was observed among the study population according to age, starting from 10.6% in the 0-9 years age group and increasing with higher age thereafter. The HEV IgG prevalence reached its highest (28.3%) in the 30-39 years, which remained at around the same level in the 40-49 years (25.0%) and 50-59 years (26.0%), with a drop of incidence in the 60-69 years age group (17.8%). The study indicates HEV infection as a public health problem in Bangladesh and suggests to ensure increased awareness to prevent andcontrol future outbreaks.Bangladesh J Med Microbiol 2007; 01 (02): 52-55

scholarly journals Diagnosis of Dengue Virus Infection by Detection of Specific Immunoglobulin M (IgM) and IgA Antibodies in Serum and Saliva

2003 ◽  
Vol 10 (2) ◽  
pp. 317-322 ◽  
Author(s):  
Angel Balmaseda ◽  
María G. Guzmán ◽  
Samantha Hammond ◽  
Guillermo Robleto ◽  
Carolina Flores ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Immunoglobulin M ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Samples ◽  
Diagnostic Modality ◽  
Iga Antibodies ◽  
Specific Immunoglobulin ◽  
Feasible Alternative

ABSTRACT To evaluate alternative approaches to the serological diagnosis of dengue virus (DEN) infection, the detection of DEN-specific immunoglobulin M (IgM) and IgA antibodies in serum and saliva specimens was assessed in 147 patients with symptoms of DEN infection seen at the Ministry of Health in Nicaragua. Seventy-two serum samples were determined to be positive for anti-DEN antibodies by IgM capture enzyme-linked immunosorbent assay, the routine diagnostic procedure. Serum and saliva specimens were obtained from 50 healthy adults as additional controls. IgM was detected in the saliva of 65 of the 72 serum IgM-positive cases, 6 of the 75 serum IgM-negative cases, and none of the control group, resulting in a sensitivity of 90.3% and a specificity of 92.0% and demonstrating that salivary IgM is a useful diagnostic marker for DEN infection. Detection of IgA in serum may be another feasible alternative for the diagnosis of DEN infection, with serum IgA found in 68 (94.4%) of the IgM-positive cases. In contrast, detection of IgA in saliva was not found to be a useful tool for DEN diagnosis in the present study. Further studies of the kinetics of antibody detection in another set of 151 paired acute- and convalescent-phase serum samples showed that DEN-specific IgA antibodies were detected in more acute-phase samples than were IgM antibodies. Thus, we conclude that DEN-specific IgA in serum is a potential diagnostic target. Furthermore, given that saliva is a readily obtainable, noninvasive specimen, detection of DEN-specific salivary IgM should be considered a useful, cheaper diagnostic modality with similar sensitivity and specificity to IgM detection in serum.

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