scholarly journals Use of Particle Agglutination in the Diagnosis of Dengue Virus Infection in Western Nepal

2012 ◽  
Vol 12 ◽  
pp. 286-289 ◽  
Author(s):  
Ramesh Pun ◽  
Krishna P Pant ◽  
Yogendra Shah ◽  
Shardulendra P Sherchand ◽  
Basu D Pandey

Dengue is an emerging vector borne viral disease in Nepal. The study was conducted to detect dengue virus specific IgM and to analyze demographic distribution of positive cases. This is a cross-sectional study carried out in western region of Nepal from August-October 2007. A total of 116 serum samples were collected from suspected dengue virus infected patients visiting hospitals and investigated by particle agglutination. Out of 116 sera, 12 (10.34%) had a positive dengue virus specific IgM. The positivity in age group below 15 years old was 23.08%, 7.32% in 15-50 years age group and 14.29% above 50 years age group. The ratio of positivity in male and female was 1:1. However, there were no statistical significance in the positivity with age (P=0.27) and sex (P=0.91). Thus, dengue has been established in western region of Nepal and diagnosis of the disease can be done by particle agglutination assay. The particle agglutination should be suggested for the diagnosis of dengue in all health centers of Nepal. Awareness programmes on prevention of dengue and surveillance of the disease should be conducted to reduce morbidity and mortality during possible outbreak of it.DOI: http://dx.doi.org/10.3126/njst.v12i0.6514 Nepal Journal of Science and Technology 12 (2011) 286-289 

2020 ◽  
Vol 30 (1) ◽  
pp. 16
Author(s):  
Ovin Nada Saputri ◽  
Viskasari P. Kalanjati ◽  
Mahrus A. Rahman

Background: Malnutrition is a common cause of morbidity and mortality amongst children with TOF. Objective: To analyze the BMI profile of children with TOF age 0 – 18 years old at Pediatric Department, Dr. Soetomo General Hospital, Surabaya. Material and method: A cross-sectional study of the BMI from the medical record of TOF patients age 0 – 18 years old at the Pediatric Department, Dr. Soetomo General Hospital, Surabaya since January 2016 to December 2017 was conducted. The data included gender, age group, demographic distribution, weight, and height. BMI was calculated by WHO formula reference; body weight (kg) divided by height squared body (m2), which was converted into z-score histogram size. BMI classification was guided by the Government’s Anthropometry Standards for Nutritional Status Assessment. Result: From 84 TOF patients, there were 43 males (51%) and 41 females (49%). The most dominant age group is 0 – 4 years old (n=47;56%). The majority of patients were from outside of Surabaya (n=64;76,2%). Based on their BMI, 36 patients (42.9%) have severe underweight, 10 patients (11.9%) were underweight, and 35 patients (41.7%) were normal, whilst the rests (3.6%) were overweight. Conclusion: In this study, the number of male patients was slightly higher than female patients. The majority of TOF patients were 0 – 4 years old and resided out town of Surabaya. The predominant BMI found was the underweight and severe underweight below of normal BMI.


2020 ◽  
Vol 30 (1) ◽  
pp. 14
Author(s):  
Ovin Nada Saputri ◽  
Viskasari P. Kalanjati ◽  
Mahrus A. Rahman

Background: Malnutrition is a common cause of morbidity and mortality amongst children with TOF. Objective: To analyze the BMI profile of children with TOF aged 0 – 18 years at Pediatric Department, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. Materials and Methods: A cross-sectional study of the BMI from the medical record of TOF patients aged 0 – 18 years at the Pediatric Department, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, since January 2016 to December 2017 was conducted. The data included gender, age group, demographic distribution, weight, and height. BMI was calculated by WHO formula reference; body weight (kg) divided by body height squared (m2), which was converted into z-score histogram. BMI classification was guided by the Government’s Anthropometry Standards for Nutritional Status Assessment. Result: From 84 TOF patients, there were 43 males (51%) and 41 females (49%). The most dominant age group was 0 – 4 years old (n=47;56%). The majority of patients were from outside of Surabaya (n=64;76.2%). Based on their BMI, 36 patients (42.9%) had severe underweight, 10 patients (11.9%) were underweight, and 35 patients (41.7%) were normal, whilst the rests (3.6%) were overweight. Conclusion: The number of male patients was slightly higher than female patients. The majority of TOF patients were 0 – 4 years old and resided out town of Surabaya. The predominant BMI found was the underweight and severe underweight below normal BMI.


2018 ◽  
pp. 21-25

Evaluación nutricional e indicadores bioquimicos en jóvenes universitarios de la provincia de Sánchez Carrión, región La Libertad  Anabel Doris González-Siccha1, Maria Virginia González Blas1, Roger Antonio Rengifo Penadillos1, Carmen Rosa Silva Correa1, Víctor Eduardo Villarreal La Torre1, George David González González2 1 Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo, Av. Juan Pablo II s/n, Trujillo – Perú 2 Universidad Particular Antenor Orrego, Av. América Sur 3145 Monserrate, Trujillo -Perú Recibido 15 de junio del 2018. Aceptado 21 de junio del 2018 DOI: https://doi.org/10.33017/ECIPeru2018.0003/ Resumen Objetivo: Determinar la evaluación nutricional e indicadores bioquímicos en jóvenes universitarios de la Provincia de Sánchez Carrión, Región La Libertad. Material y Método: Estudio descriptivo, de corte transversal y prospectivo. Participaron 29 jóvenes (26 mujeres y 03 varones), de 17-29 años de ambos sexos, habitantes de Huamachuco. Antes de su inclusión en el estudio, se requirió firma del consentimiento informado. Se determinaron las medidas antropométricas empleando variables: peso (Kg), talla (cm) e Índice de Masa Corporal. Se tomaron muestras de sangre por punción del pulpejo del dedo, se determinó hemoglobina (método Cianometahemoglobina) y hematocrito (microcentrifugación). Resultados: Los valores promedios fueron hemoglobina =15.99±1.74; hematocrito =47,73±3,48, IMC=23,66±3,78; edad 19.07±.2,45. Tuvieron IMC normal 72.42%, sobrepeso 24.14% y obesidad 3.45% que podría deberse a una sobrenutrición y dieta rica en carbohidratos. El 62.07% tuvo hemoglobina elevada con IMC normal (44.83%), sobrepeso (13.79%), obesidad (3.45 %), y el 31.03% con hemoglobina normal, sin significancia estadística (x2=0.684; P>0.05. El grupo de 17-20 años de sexo femenino tuvo 62.07% de Hb elevada (policitemia fisiológica), pero la edad no influye en la concentración de Hb. (x2=5.151; P>0.05) pero si el sexo (x2=5.59; P<0.05). El 68.96% presento hematocrito elevado con IMC normal (55.17%), sobrepeso (13.79%) y hematocrito normal (el 31.03%) sin significancia estadística (x2=3.192; P>0.05). Del 68.96% con hematocrito elevado, la mayoría según grupo etario fue de 17-20 años (62.07%), (x2=0.787; P>0.05) y según el sexo fue 58.62% mujeres y el 10.34% varones (x2=1.487;P>0.05). En las altas altitudes se estimula la eritropoyesis en compensación a la disminución parcial del oxígeno elevando la hemoglobina y hematocrito, denominado policitemia fisiológica. Se concluye que, el mayor porcentaje de jóvenes presentan valores elevados de hemoglobina y hematocrito con IMC normal debido a las altas altitudes que estimulan la eritropoyesis. Descriptores: altas altitudes, hemoglobina, hematocrito, policitemia, IMC. Abstract Objective: To determine the nutritional evaluation and biochemical indicators in university students of the Province of Sánchez Carrión, La Libertad Region. Material and Method: Descriptive, cross-sectional study and  prospective. Participants 29 young people (26 women and 03 men), 17-29 years of both sexes, inhabitants of  Huamachuco. Prior to being included in the study, signed informed consent was required. It was determined the anthropometric measures using variables: weight (Kg), height (cm) and mass index bodily. Blood samples were taken by puncture of the finger pulp, hemoglobin was determined (method Cyanomethamoglobin) and   hematocrit (microcentrifugation). Results: The average values ​​were hemoglobin = 15.99 ± 1.74; hematocrit = 47.73 ± 3.48, BMI = 23.66 ± 3.78; age 19.07 ± .2.45. They had normal BMI 72.42%, overweight 24.14% and  obesity 3.45% that could be due to overnutrition and diet rich in carbohydrates 62.07% had high hemoglobin with normal BMI (44.83%), overweight (13.79%), obesity  (3.45%), and 31.03% with normal hemoglobin,  without statistical significance (x2 = 0.684, P> 0.05, the group of 17-20 years of female sex had 62.07% high Hb (physiological polycythemia), but age does not influence the  Hb concentration. (x2 = 5.151; P> 0.05) but if the sex (x2 = 5.59; P <0.05). 68.96% presented hematocrit  elevated with normal BMI (55.17%), overweight (13.79%) and normal hematocrit (31.03%) without significance statistics (x2 = 3.192; P> 0.05). Of 68.96% with elevated hematocrit, the majority according to age group was 17-20 years (62.07%), (x2 = 0.787, P> 0.05) and according to sex was 58.62% women and 10.34% males  (x2 = 1.487; P> 0.05). At high altitudes erythropoiesis is stimulated in compensation for the partial decrease of oxygen by elevating hemoglobin and hematocrit, called physiological polycythemia. It is concluded that, higher percentage of young people have high hemoglobin and hematocrit values ​​with normal BMI due to at high altitudes that stimulate erythropoiesis. Key words: high altitudes, hemoglobin, hematocrit, polycythemia, BMI, nutritional status.


2017 ◽  
Vol 2 (2) ◽  
pp. 150-155
Author(s):  
Samyog Uprety Uprety ◽  
NPM Limbu ◽  
M Poudel ◽  
A Ghimire ◽  
VK Khanal ◽  
...  

Introduction Swine flu is an acute respiratory viral disease, caused by a strain of the influenza type A virus known as H1N1. Currently swine flu is spreading rapidly in India with a regular threat of it being spread to Nepal owing to the open borders.ObjeciveThe objective  of this study was to assess the knowledge, attiude and practice on different aspects of Swine Flu.MethodologyThis is cross-sectional study conducted in Inaruwa municipality of Sunsari district from 1st March to 10th April, 2015. A total  of 31 schools (21 private and 10 public) were identified in the municipality out of which 16 were randomly chosen for the study.ResultsTotal sample size was 260 school teachers. Majority of the respondents belong to the age group 30-39 years, about 72 % were male and 86% thought that it is a preventable disease. It was observed that females had a better attitude towards preventive measures of swine flu as compared to the males (p<0.023), but males had beertter practices as compared to the females (p<0.001).ConclusionThe preventation on practice for swine flu was not adequate. Despite having acceptable knowledge and attitude positive practices needs to be encouraged. The teachers should be well informed about the earliest appearing symptoms of swine flu. So that they can closely monitor the students and can recommend the students for medical checkup on appearance of the symptoms. Birat Journal of Health SciencesVol.2/No.1/Issue 2/ Jan - April 2017, Page: 150-155


2013 ◽  
Vol 1 (4) ◽  
pp. 224-227 ◽  
Author(s):  
B P Gupta ◽  
S K Mishra ◽  
K D Manandhar ◽  
R Malla ◽  
C S Tamarakar ◽  
...  

Dengue Virus infection is an emerging mosquito-borne disease. It is a global health problem and its expanding endemicity towards new territories is a serious concern. Relatively a new disease in Nepalese context, dengue abruptly appeared as massive outbreak in 2010, merely four years after its first introduction. It is a nagging public health problem in the low lands of Terai, expanding to new areas of Nepal in recent years.  A cross-sectional study was conducted to determine anti-Dengue IgM positive rate in Lumbini, Dhading and Chitwan district. The study was carried from June 2012 to November 2012. The total number of Serum samples was collected from 275 patients visiting hospitals with history of fever, headache and suspected DF. The samples were examined by ELISA. The anti-Dengue IgM positivity was found to be 29.09 %. The positive rate was highest in Dhading (70.37%) followed by Bharatpur (37.6%) and Lumbini (11.38%). The Dengue positive cases were higher in males (32.5 %) than female (24.8 %). The highest positive cases (41.6%) were from age group less than 15 years. Dengue has substantial expansion in Western and Far Western Terai region of Nepal which was limited to the middle Terai region in the past and mostly infects older people.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9135  Int J Appl Sci Biotechnol, Vol. 1(4): 224-227


2018 ◽  
pp. 19-23

Evaluación nutricional e indicadores bioquimicos en jóvenes universitarios de la provincia de Sánchez Carrión, región La Libertad Anabel Doris González-Siccha1, Maria Virginia González Blas1, Roger Antonio Rengifo Penadillos1, Carmen Rosa Silva Correa1, Víctor Eduardo Villarreal La Torre1, George David González González2 1 Facultad de Farmacia y Bioquímica, Universidad Nacional de Trujillo, Av. Juan Pablo II s/n, Trujillo - Perú 2 Universidad Particular Antenor Orrego, Av. América Sur 3145 Monserrate, Trujillo -Perú Recibido 15 de junio del 2018. Aceptado 21 de junio del 2018 Resumen Objetivo: Determinar la evaluación nutricional e indicadores bioquímicos en jóvenes universitarios de la Provincia de Sánchez Carrión, Región La Libertad. Material y Método: Estudio descriptivo, de corte transversal y prospectivo. Participaron 29 jóvenes (26 mujeres y 03 varones), de 17-29 años de ambos sexos, habitantes de Huamachuco. Antes de su inclusión en el estudio, se requirió firma del consentimiento informado. Se determinaron las medidas antropométricas empleando variables: peso (Kg), talla (cm) e Índice de Masa Corporal. Se tomaron muestras de sangre por punción del pulpejo del dedo, se determinó hemoglobina (método Cianometahemoglobina) y hematocrito (microcentrifugación). Resultados: Los valores promedios fueron hemoglobina =15.99±1.74; hematocrito =47,73±3,48, IMC=23,66±3,78; edad 19.07±.2,45. Tuvieron IMC normal 72.42%, sobrepeso 24.14% y obesidad 3.45% que podría deberse a una sobrenutrición y dieta rica en carbohidratos. El 62.07% tuvo hemoglobina elevada con IMC normal (44.83%), sobrepeso (13.79%), obesidad (3.45 %), y el 31.03% con hemoglobina normal, sin significancia estadística (x2=0.684; P>0.05. El grupo de 17-20 años de sexo femenino tuvo 62.07% de Hb elevada (policitemia fisiológica), pero la edad no influye en la concentración de Hb. (x2=5.151; P>0.05) pero si el sexo (x2=5.59; P<0.05). El 68.96% presento hematocrito elevado con IMC normal (55.17%), sobrepeso (13.79%) y hematocrito normal (el 31.03%) sin significancia estadística (x2=3.192; P>0.05). Del 68.96% con hematocrito elevado, la mayoría según grupo etario fue de 17-20 años (62.07%), (x2=0.787; P>0.05) y según el sexo fue 58.62% mujeres y el 10.34% varones (x2=1.487;P>0.05). En las altas altitudes se estimula la eritropoyesis en compensación a la disminución parcial del oxígeno elevando la hemoglobina y hematocrito, denominado policitemia fisiológica. Se concluye que, el mayor porcentaje de jóvenes presentan valores elevados de hemoglobina y hematocrito con IMC normal debido a las altas altitudes que estimulan la eritropoyesis. Descriptores: altas altitudes, hemoglobina, hematocrito, policitemia, IMC. Abstract Objective: To determine the nutritional evaluation and biochemical indicators in university students of the Province of Sánchez Carrión, La Libertad Region. Material and Method: Descriptive, cross-sectional study and prospective. Participants 29 young people (26 women and 03 men), 17-29 years of both sexes, inhabitants of Huamachuco. Prior to being included in the study, signed informed consent was required. It was determined the anthropometric measures using variables: weight (Kg), height (cm) and mass index bodily. Blood samples were taken by puncture of the finger pulp, hemoglobin was determined (method Cyanomethamoglobin) and hematocrit (microcentrifugation). Results: The average values were hemoglobin = 15.99 ± 1.74; hematocrit = 47.73 ± 3.48, BMI = 23.66 ± 3.78; age 19.07 ± .2.45. They had normal BMI 72.42%, overweight 24.14% and obesity 3.45% that could be due to overnutrition and diet rich in carbohydrates 62.07% had high hemoglobin with normal BMI (44.83%), overweight (13.79%), obesity (3.45%), and 31.03% with normal hemoglobin, without statistical significance (x2 = 0.684, P> 0.05, the group of 17-20 years of female sex had 62.07% high Hb (physiological polycythemia), but age does not influence the Hb concentration. (x2 = 5.151; P> 0.05) but if the sex (x2 = 5.59; P <0.05). 68.96% presented hematocrit elevated with normal BMI (55.17%), overweight (13.79%) and normal hematocrit (31.03%) without significance statistics (x2 = 3.192; P> 0.05). Of 68.96% with elevated hematocrit, the majority according to age group was 17-20 years (62.07%), (x2 = 0.787, P> 0.05) and according to sex was 58.62% women and 10.34% males (x2 = 1.487; P> 0.05). At high altitudes erythropoiesis is stimulated in compensation for the partial decrease of oxygen by elevating hemoglobin and hematocrit, called physiological polycythemia. It is concluded that, higher percentage of young people have high hemoglobin and hematocrit values with normal BMI due to at high altitudes that stimulate erythropoiesis. Key words: high altitudes, hemoglobin, hematocrit, polycythemia, BMI, nutritional status.


Author(s):  
Ravi Sankar Deekala ◽  
Ravi Prabhu G. ◽  
Altaf Hussain R. ◽  
Saritha Kadari

Background: Some of the community surveys have documented that between three and six decades, prevalence of hypertension has increased by about 30 times in urban people and by about 10 times among the rural area people. Urban population of developing countries is showing similar prevalence to that of developed countries. Treating hypertension has been associated with 15% reduction in incidence of myocardial infarction and 40% reduction of stroke. The objectives of the study were to estimate the prevalence of hypertension among adults aged between 30-50 years in selected urban slum areas of Tirupati and to know the associated important risk factors with hypertension in the study subjects.Methods: A community based cross sectional study was conducted in urban slum community of Tirupati, to estimate the prevalence and its risk factors of Hypertension by using the JNC VII criteria. This study was conducted during January 2012 to February 2013, among 2136 subjects of 30-50 years age group. Results: The prevalence of hypertension was found 481 (22.5%), majority of them belongs to the age group of 45-50 years 212 (44.0%) and lowest in 30-35 years 45 (9.3%). Hypertension was predominant in males 186 (30.1%) than females 295 (19.4%). Conclusions: This study found that there is increasing trend of prevalence of hypertension in the urban community. This study suggests to health planners to take remedial measures to address growing hypertension in the community through health education about lifestyle changes, dietary modification, and avoidance of stress. 


2019 ◽  
Vol 9 (1) ◽  
pp. 348-350
Author(s):  
ANUP SINGH

Objective: To correlate the level of Dickkopf-1 (DKK-1) and its role in rheumatoid arthritis (RA) patients of elderly age group. Methods:  Serum samples were collected elderly RA patients fulfilling the inclusion criteria coming to Geriatric outdoor clinic between June 2018 to September 2018. DKK-1 was detected by ELISA. Serum C-reactive protein (CRP) levels, erythrocyte sedimentation rates (ESR), rheumatoid factor (RF) titers, and anti-cyclic citrullinated peptide antibody were also measured in patients with RA. Results: Twenty one patients were enrolled in study period. The serum level of DKK-1 was significantly higher in patients with severe RA with high DAS score (p < 0.01); The serum DKK-1 level was correlated with T-score (r= -0.588; p = 0.005), Z-Score(r= -0.458, p = 0.037) and the larson score of radiologic change (r = +0.673, p = 0.001) in RA, however not correlated significantly with ESR, CRP. Conclusion: DKK-1 may serve as a biomarker of bone erosion and correlates with the disease activity in RA patients of elderly age group. Keyword: DKK 1, Rheumatoid arthritis, Geriatric, Osteoporosis.


2019 ◽  
Vol 6 (1) ◽  
pp. 18-23
Author(s):  
Letícia Cardoso Lucena ◽  
Alexandre Arguelio Souto ◽  
Larissa Cardoso Lucena ◽  
Tathiana Nascimento Marques

Introdução – A dengue é uma doença viral infecciosa, transmitida principalmente pelo mosquito Aedes aegypt e de grande incidência no Brasil. É uma enfermidade de grande potencial epidêmico, representando um grave problema de saúde pública. Objetivo – avaliar o perfil dos casos de dengue no município de Porto Nacional - TO no período de janeiro de 2010 a outubro de 2018. Metodologia – Este é um estudo descritivo, quantitativo e transversal realizado através da avaliação de dados do SINAN sobre a dengue no período de 2010-2018 e de levantamento bibliográfico em bases de dados eletrônicas. Resultados – Com a análise dos dados observou-se a notificação de 5.085 casos de dengue no período, a maioria no ano de 2010. A prevalência foi no sexo feminino (53,9%) e na faixa etária de 20-29 anos (22%), com maior ocorrência no período de janeiro a abril, época de índices pluviométricos mais altos, facilitando a procriação do vetor. Outrossim, as localidades com os maiores números de notificação são áreas mais abastadas e com menos condições sanitárias.    Conclusão – Ainda há ocorrência de subnotificação, além de problemas nos serviços de saúde, sendo necessária adoção de medidas de prevenção e combate, e melhorias nos serviços de saúde. Palavras-chave: Dengue; Arboviroses; Saúde pública; Epidemia. ABSTRACT Introduction – Dengue is an infectious viral disease, transmitted mainly by the Aedes aegypt mosquito and of great incidence in Brazil. It is a disease of great epidemic potential, representing a serious public health problem. Objective – To evaluate the profile of dengue cases in the municipality of Porto Nacional - TO from January 2010 to October 2018. Methodology – This is a descriptive, quantitative and cross - sectional study carried out through the evaluation of SINAN data on dengue in period of 2010-2018 and a bibliographic survey in electronic databases. Results – Data were analyzed in a study of 5,085 dengue cases in the period, most of them in 2010. The prevalence was in the female sex (53.9%) and in the age group of 20-29 years (22 %), with higher occurrence in the period from January to April, season of higher rainfall indexes, facilitating the procreation of the vector. In addition, the locations with the highest reporting numbers are more affluent areas with less sanitary conditions. Conclusion – There is still an underreporting, besides problems in the health services, being necessary to adopt measures of prevention and combat, and improvements in health services. Keywords: Dengue; Arboviruses; Public health; Epidemic.


2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Hatim H. Abraheem ◽  
Amira M. Elhassan ◽  
Mohammed O. Hussien ◽  
Khalid A. Enan ◽  
Azza B. Musa ◽  
...  

Bluetongue (BT) is an infectious, noncontagious, vector-borne viral disease that affects wild and domestic ruminants transmitted by Culicoides spp. A cross-sectional study was carried out during the period 2016-2017 in Gadarif state. A total of 276 sera samples were collected from camels in six localities of Gadarif state, eastern Sudan, to investigate bluetongue virus (BTV) seroprevalence and associated risk factors of BTV infection including age, sex, breed, locality, and ecology of the region. Enzyme-linked immunosorbent assay (ELISA) was used for estimation of BTV seroprevalence rate. The overall BTV seroprevalence rate was 96.7% in the study area ranging from 93.5% to 100% in six screened localities with no significant differences. The findings revealed similar BTV seroprevalence rates in both males and females, but high rates were found in age group of less than one year and two to three years with estimated 100%. However, the lowest seroprevalence was found in the age group of five to four years with estimated BTV to be 92.3%. BTV seropositivity was not found to be statistically associated with examined different camel breeds which revealed 93%, 94.4%, 97.6%, and 97.8% seroprevalence in Bushari, Rashide, Arabi, and Anafi, breeds, respectively. Epidemiology of BTV assessment according to the ecology of the area showed high BTV seroprevalence in desert and savanna with estimated 100% and lower BTV seroprevalence in arid and rich savanna with estimated 94.8% and 95.7%, respectively. There was no significant association between BTV ELISA positivity and sex, breed, and ecology of the area.


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