Histopathological profile of cervical biopsy specimens at Paropakar Maternity and Women’s Hospital

Aims: The lesions at uterine cervix cannot be always established only with cytology. Thus, it is very important that cytological abnormality be subsequently correlated with biopsy for confirmation of cervical lesion. Thus it is to see histopathological findings of different types of cervical pathology in cervical biopsies.Methods: This is retrospective analysis of histology result of 1184 cervical biopsy specimens from 2011 to 2016.Results: Out of 1108 histologically adequate cervical specimens, benign cervical lesion formed the major part (44.76%)followed by cervical inflammatory lesion (27.43 %). Malignant and borderline cervical lesion constituted 14.35% and 13.44% respectively; 6.4% biopsy samples were inadequate to report.CIN I was common among borderline lesions followed by CIN III. The most common cervical malignancy was squamous cell type and mostly moderately differentiated.Conclusions:Benign cervical lesions were the most common cervical lesions followed by inflammatory conditions. Among borderline cervical lesions CIN I was commonly found followed by CIN III.

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Raman spectroscopic study of cervical precancerous lesions and cervical cancer

Lasers in Medical Science ◽

10.1007/s10103-020-03218-5 ◽

2021 ◽

Author(s):

Jing Wang ◽

Cheng-Xia Zheng ◽

Cai-Ling Ma ◽

Xiang-Xiang Zheng ◽

Xiao-Yi Lv ◽

...

Keyword(s):

Cervical Cancer ◽

Squamous Cell Carcinoma ◽

Raman Spectra ◽

Precancerous Lesions ◽

Cervical Squamous Cell Carcinoma ◽

Cervical Lesions ◽

Cin Iii ◽

Cervical Precancerous Lesions ◽

Biochemical Components ◽

The Difference

AbstractEarly detection of cervical lesions, accurate diagnosis of cervical lesions, and timely and effective therapy can effectively avoid the occurrence of cervical cancer or improve the survival rate of patients. In this paper, the spectra of tissue sections of cervical inflammation (n = 60), CIN (cervical intraepithelial neoplasia) I (n = 30), CIN II (n = 30), CIN III (n = 30), cervical squamous cell carcinoma (n = 30), and cervical adenocarcinoma (n = 30) were collected by a confocal Raman micro-spectrometer (LabRAM HR Evolution, Horiba France SAS, Villeneuve d’Ascq, France). The Raman spectra of six kinds of cervical tissues were analyzed, the dominant Raman peaks of different kinds of tissues were summarized, and the differences in chemical composition between the six tissue samples were compared. An independent sample t test (p ≤ 0.05) was used to analyze the difference of average relative intensity of Raman spectra of six types of cervical tissues. The difference of relative intensity of Raman spectra of six kinds of tissues can reflect the difference of biochemical components in six kinds of tissues and the characteristic of biochemical components in different kinds of tissues. The classification models of cervical inflammation, CIN I, CIN II, CIN III, cervical squamous cell carcinoma, and cervical adenocarcinoma were established by using a support vector machine (SVM) algorithm. Six types of cervical tissues were classified and identified with an overall diagnostic accuracy of 85.7%. This study laid a foundation for the application of Raman spectroscopy in the clinical diagnosis of cervical precancerous lesions and cervical cancer.

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Predictors for progression of two different types of cervical lesions in rheumatoid arthritis treated with biologic agents

Journal of Orthopaedic Science ◽

10.1016/j.jos.2018.09.001 ◽

2019 ◽

Vol 24 (2) ◽

pp. 214-218

Author(s):

Takashi Kaito ◽

Shiro Ohshima ◽

Hiroyasu Fujiwara ◽

Takahiro Makino ◽

Shota Takenaka ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Biologic Agents ◽

Cervical Lesions ◽

Different Types

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Variability of Pathologists' Utilization of p16 and Ki-67 Immunostaining in the Diagnosis of Cervical Biopsies in Routine Pathology Practice and Its Impact on the Frequencies of Cervical Intraepithelial Neoplasia Diagnoses and Cytohistologic Correlations

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2012-0472-oa ◽

2014 ◽

Vol 138 (1) ◽

pp. 76-87 ◽

Cited By ~ 12

Author(s):

Charanjeet Singh ◽

J. Carlos Manivel ◽

Alexander M. Truskinovsky ◽

Kay Savik ◽

Samy Amirouche ◽

...

Keyword(s):

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Lower Range ◽

Ki 67 ◽

Detection Rates ◽

Cervical Biopsy ◽

Biopsy Specimens ◽

Frequency Of Use ◽

Lower Variability ◽

The Impact

Context.—The use of p16 in cervical biopsies improves the accuracy of cervical intraepithelial neoplasia (CIN) diagnosis and grading and decreases its interpathologist variability. Objective.—To determine the impact of the frequency of use of p16 immunostains in cervical biopsies on pathologists' diagnoses of CIN grade 1 and grade 2 or above (CIN1 and CIN2+) and on cytohistologic correlations. Design.—We identified all cervical biopsy specimens with cytologic correlations subjected or not to p16 staining from January 1, 2005, to September 30, 2010; calculated each pathologist's percentage of p16 use; and correlated it with their major cytohistologic discrepancy rates, CIN2+ diagnoses, and CIN1/CIN2+ ratios. Results.—During the study period, each of the 23 pathologists interpreted 59 to 1811 (mean, 518) of 11 850 cervical biopsy specimens, used p16 for 0% to 21.31% (mean, 10.14%) of these, had CIN2+ detection rates of 9.5% to 24.1% (mean, 18.9%), and CIN1/CIN2+ ratios of 0.7 to 4.5 (mean, 1.5). Compared to the 12 “low users” of p16, who used p16 fewer times than the institution's mean for p16 use, the 11 “high users” of p16 diagnosed more biopsies (8391 versus 3459), had a lower rate of major cytohistologic discrepancies (12.62% versus 14.92%, P < .001), a higher rate of CIN2+ diagnoses (19.9% versus 16.4%, P < .001), a lower range of CIN2+ rates (15.0%–23.1% versus 9.5%–24.1%), and lower CIN1/CIN2+ ratios (1.2 versus 2.3). Conclusions.—We found a high intrainstitutional variability of p16 use in cervical biopsies, CIN2+ rates, and CIN1/CIN2+ ratios. Use of p16 for greater than 10% of cervical biopsies was associated with improved cytohistologic correlation rates and with lower variability in the frequencies of histologic diagnoses.

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Kappa statistics to measure interrater and intrarater agreement for 1790 cervical biopsy specimens among twelve pathologists: Qualitative histopathologic analysis and methodologic issues

Yearbook of Pathology and Laboratory Medicine ◽

10.1016/s1077-9108(08)70532-2 ◽

2008 ◽

Vol 2008 ◽

pp. 13-15

Author(s):

S.A. Raab

Keyword(s):

Kappa Statistics ◽

Cervical Biopsy ◽

Biopsy Specimens ◽

Histopathologic Analysis

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Comparison of Fracture Resistance of Teeth Presenting non-carious Cervical Lesions, Restored with Different Composite Materials

Materiale Plastice ◽

10.37358/mp.20.1.5339 ◽

2020 ◽

Vol 57 (1) ◽

pp. 299-305

Author(s):

Ruxandra Margarit ◽

Livia Alice Tanasescu ◽

Dana Bodnar ◽

Camelia Gabriela Ion ◽

Mihai Burlibasa ◽

...

Keyword(s):

Composite Materials ◽

Fracture Resistance ◽

Mean Value ◽

P Value ◽

Cervical Lesions ◽

Nano Composite ◽

Commercial Grade ◽

Different Types ◽

Group A ◽

Group B

The aim of our study was to compare the fracture resistance of teeth presenting non-carious cervical lesions restored with different types of esthetic composite materials. 20 extracted unspoiled maxillary first molars were mechanically cleaned and immersed in saline solution containing 0.1% thymol at 4�C for a period of 48 hours. Cervical cavities with a cervical-occlusal diameter of 2 mm and a mesial-distal diameter of 3mm were filled with ormocer, flow nano-composite, nano-composite and compomer. Fracture resistance was tested with a universal loading machine (Lloyd Instruments), with a maximum force of 5 kN and a crosshead speed of 1.0 mm/min; the authors used NEXYGEN Data Analysis Software and ANOVA method. For the group A (commercial grade ormocer), the smallest load that determined the sample failure was 650 N and the highest load was 1050 N, the mean value being 858 N � 150.89 N. For the group B (commercial grade flow nano-composite), the smallest load is 530 N, the highest load is 800 N, mean value being 654 N � 112.6 N. For the group C (commercial grade nano-composite), the smallest load is 680 N, the highest load is 1200 N, mean value being 926 N � 209.35 N. For the group D (commercial grade compomer), the smallest load is 1100 N, highest load is 1250N, mean value being 1180 N � 62.04 N. A p value of 0.000311 (p[0.05) shows that there are significant differences between the four groups. Conclusions. The best fracture resistance of teeth presenting non-carious cervical lesions, restored with different types of esthetic composite materials is assured by the compomer, followed by the nano-composite, which proved to be superior to ormocer. The flow nano-composite gives the lowest fracture resistance.

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Cytohistological correlation of conventional Papanicolaou smears in cervical neoplasia at a tertiary care hospital of Nepal

Journal of Pathology of Nepal ◽

10.3126/jpn.v9i1.23372 ◽

2019 ◽

Vol 9 (1) ◽

pp. 1475-1479

Author(s):

Anuj Poudel ◽

Prahar Dahal

Keyword(s):

Uterine Cervix ◽

Pap Smear ◽

Cervical Screening ◽

Tertiary Care ◽

Cost Effective ◽

Cervical Cytology ◽

Pap Smears ◽

Care Hospital ◽

Cervical Lesions ◽

Cervical Biopsy

Background: Conventional Cervical Cytology is the most widely used cervical cancer screening test. The incidence of cervical carcinoma is incredibly high in developing countries due to lack of proper knowledge. The extensive use of cervical screening with Papanicolau smears had considerably increased the detection of precancerous and cancerous lesion of uterine cervix. Study was conducted to evaluate cytohistological correlation and to calculate concordance and discordance of Pap smear in detection of precancerous and cancerous lesions of uterine cervix. Materials and Methods: A total of 54 samples reported in Pap smear as cervical intraepithelial lesions and suspicious for invasive malignancies were selected and correlated with corresponding histopathological findings. It was an observational study done for a period of 18 months from November 2016 to May 2018 at UCMS-TH. All the statistical evaluation was done by using SPSS version 20. Results: During the study period 54 cases were evaluated. Atypical Squamous Cell of Undetermined Significance was the common abnormal findings in the Pap smear with frequency of 26 (48.1%). Out of total 54 cases of Pap smears, 30 (55.55%) were concordant while discrepancies occurred in 24(44.44%) cases with cervical biopsy. Conclusions: The study revealed a good correlation between cervical cytology and cervical biopsy. We also concluded that conventional Pap smear is a cost effective test for the early detection of precancerous and cancerous cervical lesions.

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Identification of genital human papillomaviruses in cervical biopsy specimens: Segregation of specific virus types in specific clinicopathologic lesions

International Journal of Cancer ◽

10.1002/ijc.2910610104 ◽

1995 ◽

Vol 61 (1) ◽

pp. 13-22 ◽

Cited By ~ 72

Author(s):

Toshihiko Matsukura ◽

Motoyasu Sugase

Keyword(s):

Human Papillomaviruses ◽

Cervical Biopsy ◽

Biopsy Specimens ◽

Specific Virus

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State of HPV16 integration in Lithuanian women with cervical neoplasia

Open Medicine ◽

10.2478/s11536-011-0004-6 ◽

2011 ◽

Vol 6 (2) ◽

pp. 205-212

Author(s):

Agne Sepetiene ◽

Zivile Gudlevicienė ◽

Zana Bumbuliene ◽

Grazina Drasutiene ◽

Janina Didziapetriene

Keyword(s):

Cervical Cancer ◽

Hpv Infection ◽

Rapid Progression ◽

The European Union ◽

Cervical Lesions ◽

Hpv 16 ◽

Hpv Dna ◽

Cin Iii ◽

Cell Genome ◽

Virus Integration

AbstractCervical cancer morbidity and mortality in Lithuania is one of the biggest in the European Union. The main risk factor of cervical cancer is human papillomavirus (HPV). The deletion of the HPV E2 gene influences HPV DNA integration into the cell genome, as well as a rapid progression of cervical lesions. The purpose of this study is to determine HPV, its types, and HPV 16 integration in different grades of cervical intraepithelial neoplasias (CIN). 253 women with cytological lesions were involved in the study. After a histology, 31 women were diagnosed with CIN I, 35 with CIN II, and 51 with CIN III. The biggest prevalence of HPV infection was detected in women younger than 25 years old (69.7%) and in women with CIN II (90.9%). HPV 16 was detected in 67.8% of all cases, with the highest prevalence in CIN III (84.4%). A partial integration form was detected in 65.0% of HPV 16 infected women, a complete virus integration in 26.5%, and an episomal form in 8.4% of cases. Our study concludes that in all the cases confirmed using a histology, the partial virus integration form of CIN was identified the most. It was less frequently detected in CIN I cases (60.0%), but more frequently in CIN II and CIN III cases (72.8 and 69.3%, respectively).

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Review of Cervical Carcinoma Screening Program in Tamil Nadu – Current Trend and Recommendations from a Histopathologist’s Viewpoint

The Journal of South Asian Federation of Menopause Societies ◽

10.5005/jp-journals-10032-1095 ◽

2017 ◽

Vol 5 (1) ◽

pp. 1-7

Author(s):

Shifa S Ibrahim ◽

Kamaleshwari Kesavaraj ◽

Muthumani Arun ◽

SA Mohamed Ameen ◽

Raasi Sankar

Keyword(s):

Cervical Carcinoma ◽

Hpv Vaccine ◽

Tamil Nadu ◽

Visual Inspection ◽

Screening Program ◽

Current Trend ◽

High Grade Dysplasia ◽

High Grade ◽

Cervical Biopsy ◽

Biopsy Specimens

ABSTRACT Objectives Cervical carcinoma, the commonest carcinoma affecting Indian females, is caused by human papilloma virus (HPV) infection. Primordial prevention and primary prevention with HPV vaccine and cancer screening respectively, can go a long way in preventing this carcinoma. The health system project in Tamil Nadu has done a commendable job in reducing the disease burden by introducing screening programs for cervical carcinoma at the grassroots level, way back in 2005. This study was done to evaluate the cervical biopsy specimens received as a part of this program to compute its incidence, compare the incidence among various districts, and suggest future directions based on our observations. Materials and methods From visual inspection with acetic acid/visual inspection with Lugol’s iodine positive cervical biopsy specimens, 506 were chosen randomly from various districts. Based on histopathological examination, incidence of individual lesions and district-wise incidence were calculated. Predictive factors that determine the progression of these lesions were analyzed based on the literature. Results Out of the 506 cervical biopsy specimens, 34 were unsatisfactory. The incidence of high-grade dysplasia peaked around 31 to 40 years, and squamous cell carcinoma peaked among 51 to 60 years. Madurai ranked high in the incidence of both high-grade dysplasia and carcinoma. Conclusion Incidence of dysplasia and carcinoma in our study was comparable to those seen in the literature. Integration of HPV deoxyribonucleic acid studies into the program can increase the detection rate, detect the progressors, help to identify the HPV species prevalent in an area, and aid in formulating cost-effective HPV vaccine cocktail. How to cite this article Ibrahim SS, Kesavaraj K, Arun M, Ameen SAM, Sankar R. Review of Cervical Carcinoma Screening Program in Tamil Nadu – Current Trend and Recommendations from a Histopathologist’s Viewpoint. J South Asian Feder Menopause Soc 2017;5(1):1-7.

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