scholarly journals Raman spectroscopic study of cervical precancerous lesions and cervical cancer

2021 ◽  
Author(s):  
Jing Wang ◽  
Cheng-Xia Zheng ◽  
Cai-Ling Ma ◽  
Xiang-Xiang Zheng ◽  
Xiao-Yi Lv ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Raman Spectra ◽  
Precancerous Lesions ◽  
Cervical Squamous Cell Carcinoma ◽  
Cervical Lesions ◽  
Cin Iii ◽  
Cervical Precancerous Lesions ◽  
Biochemical Components ◽  
The Difference

AbstractEarly detection of cervical lesions, accurate diagnosis of cervical lesions, and timely and effective therapy can effectively avoid the occurrence of cervical cancer or improve the survival rate of patients. In this paper, the spectra of tissue sections of cervical inflammation (n = 60), CIN (cervical intraepithelial neoplasia) I (n = 30), CIN II (n = 30), CIN III (n = 30), cervical squamous cell carcinoma (n = 30), and cervical adenocarcinoma (n = 30) were collected by a confocal Raman micro-spectrometer (LabRAM HR Evolution, Horiba France SAS, Villeneuve d’Ascq, France). The Raman spectra of six kinds of cervical tissues were analyzed, the dominant Raman peaks of different kinds of tissues were summarized, and the differences in chemical composition between the six tissue samples were compared. An independent sample t test (p ≤ 0.05) was used to analyze the difference of average relative intensity of Raman spectra of six types of cervical tissues. The difference of relative intensity of Raman spectra of six kinds of tissues can reflect the difference of biochemical components in six kinds of tissues and the characteristic of biochemical components in different kinds of tissues. The classification models of cervical inflammation, CIN I, CIN II, CIN III, cervical squamous cell carcinoma, and cervical adenocarcinoma were established by using a support vector machine (SVM) algorithm. Six types of cervical tissues were classified and identified with an overall diagnostic accuracy of 85.7%. This study laid a foundation for the application of Raman spectroscopy in the clinical diagnosis of cervical precancerous lesions and cervical cancer.

scholarly journals Faktor Risiko yang Berhubungan dengan Kejadian Lesi Prakanker Serviks (IVA+) di Puskesmas Bakunase Kota Kupang Tahun 2019

2020 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Rambu Aji Paremajangga ◽  
Honey I. Ndoen ◽  
Yuliana R. Riwu
Keyword(s):  
Risk Factors ◽  
Cervical Cancer ◽  
Early Detection ◽  
Health Center ◽  
Precancerous Lesions ◽  
Hpv Vaccination ◽  
Cervical Lesions ◽  
First Marriage ◽  
Age At First Marriage ◽  
Cervical Precancerous Lesions

Servical cancer is the most common cancer in women in the world and ranks second most cause of death after cardiovascular disease. Early detection of cervical cancer is done through IVA method. Early detection of servical cancer in Kupang City Health Office in 2017 totaling 1.757 woman and 58 women are positive for cervical precancerous lesions with highest cases in the Bakunase Health Center as many as 38 people. The purpose of this study was to analyze the factors of age at first marriage, habits of changing partners, parity and pathological vaginal history of the occurrence of cervical precancerous lesions (IVA +) in Bakunase Health Center Kupang City in 2019. This type of research is an analytic survey with a case control research design. To analyze the relationship between the variables used the chi square test and the magnitude of risk factors calculated Odds Ratio (OR) values. The results showed that there was a relationship between age at first marriage p = 0.038 (OR = 2.979; 95% CI 1.164-7.622), parity p = 0.021 (OR = 3.322; 95% CI: 1.293-8.538), pathological vaginal discharge p = 0.021 ( OR = 3,375; 95% CI: 1,303-8,744), while the habit of changing sexual partners is not a risk factor associated with the incidence of precancerous cervical lesions p = 0.358 (OR = 4.353; 95% CI 0.463-40.898. Suggestions for Kupang City Bakunase Health Center to conduct counseling about risk factors and dangers of cervical cancer, create an HPV vaccination program and encourage every woman to vaccinate. For women-only communities to avoid risk factors and make periodic early detection for those who have already married.  

scholarly journals HUBUNGAN ANTARA LESI PRAKANKER SERVIKS DENGAN SOSIODEMOGRAFI PEREMPUAN DI KECAMATAN LEGOK KABUPATEN TANGERANG TAHUN 2018

2020 ◽  
Vol 4 (1) ◽  
pp. 193 ◽  
Author(s):  
Destiyana Cika Claritha ◽  
Chandra Dewi ◽  
Shintia Christina
Keyword(s):  
Breast Cancer ◽  
Cervical Cancer ◽  
Analytical Study ◽  
Precancerous Lesions ◽  
Total Sample ◽  
Cervical Lesions ◽  
Cervical Intraepithelial Lesions ◽  
Cervical Precancerous Lesions ◽  
Precancerous Cervical Lesions ◽  
Intraepithelial Lesions

Cervical and breast cancer are the highest prevalence cancer in Indonesia in 2013 which caused death to Indonesian women, cervical cancer by 0.8‰ and breast cancer by 0.5‰. Precancerous lesions of the cervix also known as cervical intraepithelial lesions (Cervical Intraepithelial Neoplastic) are the beginning of changes to cervical carcinoma. In general, precancerous lesions of the cervix originate from the squamous columnar junction in the uterine cervix, which undergoes the process of metaplasia. This study aims to determine whether there is a relationship between precancerous cervical lesions and sociodemography of women in Kecamatan Legok Kabupaten Tangerang in 2018. This study uses an analytical study with cross sectionaI approach. The population is all women in Kecamatan Legok Kabupaten Tangerang who had sexual relations. Total sample are 47 people. Keywords: cervical cancer, cervical precancerous lesions, parity ABSTRAKPenyakit kanker serviks dan payudara merupakan penyakit kanker dengan prevalensi tertinggi di Indonesia pada tahun 2013 yang menyebabkan kematian pada perempuan Indonesia, yaitu kanker serviks sebesar 0,8‰ dan kanker payudara sebesar 0,5‰. Lesi prakanker pada serviks dikenal juga dengan sebutan lesi intraepitelial serviks (Cervical Intraephitelial Neoplasia) merupakan awal dari perubahan menuju karsinoma serviks. Pada umumnya lesi prakanker serviks ini berawal dari daerah squamocolumnar junction pada serviks uteri yang mengalami proses metaplasia. Penelitian ini bertujuan untuk mengetahui adakah hubungan antara lesi prakanker serviks dengn sosiodemografi perempuan di Kecamatan Legok Kabupaten Tangerang tahun 2018. Penelitian ini merupakan studi analitik dengan pendekatan cross sectionaI. Populasi penelitian adalah semua perempuan di Kecamatan Legok Kabupaten Tangerang yang sudah berhubungan seksual. Total sampel 47 orang.

scholarly journals Prevalence and Risk Factors Associated with Precancerous Cervical Cancer Lesions among HIV-Infected Women in Resource-Limited Settings

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Peter Memiah ◽  
Wangeci Mbuthia ◽  
Grace Kiiru ◽  
Solomon Agbor ◽  
Francesca Odhiambo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cervical Cancer ◽  
Logistic Regression ◽  
Precancerous Lesions ◽  
Multivariate Logistic Regression Model ◽  
Cervical Lesions ◽  
Resource Limited Settings ◽  
Resource Limited ◽  
Cervical Precancerous Lesions ◽  
Associated Risk Factors

Objective. To assess the prevalence and identified associated risk factors for precancerous cervical cancer lesions among HIV-infected women in resource-limited settings in Kenya.Methods. HIV-infected women attending the ART clinic at the Nazareth Hospital ART clinic between June 2009 and September 2010. Multivariate logistic regression model with odds ratios and 95% confidence intervals (CI) were estimated after controlling for important covariates.Result. A total of 715 women were screened for cervical cancer. The median age of the participants was 40 years (range 18–69 years). The prevalence of precancerous lesions (CINI, CINII, CIN III, ICC) was 191 (26.7%). After controlling for other variables in logistic regression analysis, cervical precancerous lesions were associated with not being on ART therapy; whereby non-ART were 2.21 times more likely to have precancerous lesions than ART patients [(aOR)=2.21, 95% CI (1.28–3.83)].Conclusion. The prevalence of precancerous cervical lesions was lower than other similar settings. It is recommended that cancer screening of HIV-infected women should be an established practice. Availability and accessibility of these services can be done through their integration into HIV. Prompt initiation of HAART through an early enrollment into care has an impact on reducing the prevalence and progression of cervical precancerous lesions.

scholarly journals The application of CRISPR/Cas9 system in cervical carcinogenesis

2021 ◽  
Author(s):  
Chun Gao ◽  
Ping Wu ◽  
Lan Yu ◽  
Liting Liu ◽  
Hong Liu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Transgenic Mice ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Precancerous Lesions ◽  
Cancer Cell Lines ◽  
Cervical Carcinogenesis ◽  
Cervical Precancerous Lesions

AbstractIntegration of high-risk HPV genomes into cellular chromatin has been confirmed to promote cervical carcinogenesis, with HPV16 being the most prevalent high-risk type. Herein, we evaluated the therapeutic effect of the CRISPR/Cas9 system in cervical carcinogenesis, especially for cervical precancerous lesions. In cervical cancer/pre-cancer cell lines, we transfected the HPV16 E7 targeted CRISPR/Cas9, TALEN, ZFN plasmids, respectively. Compared to previous established ZFN and TALEN systems, CRISPR/Cas9 has shown comparable efficiency and specificity in inhibiting cell growth and colony formation and inducing apoptosis in cervical cancer/pre-cancer cell lines, which seemed to be more pronounced in the S12 cell line derived from the low-grade cervical lesion. Furthermore, in xenograft formation assays, CRISPR/Cas9 inhibited tumor formation of the S12 cell line in vivo and affected the corresponding protein expression. In the K14-HPV16 transgenic mice model of HPV-driven spontaneous cervical carcinogenesis, cervical application of CRISPR/Cas9 treatment caused mutations of the E7 gene and restored the expression of RB, E2F1, and CDK2, thereby reversing the cervical carcinogenesis phenotype. In this study, we have demonstrated that CRISPR/Cas9 targeting HPV16 E7 could effectively revert the HPV-related cervical carcinogenesis in vitro, as well as in K14-HPV16 transgenic mice, which has shown great potential in clinical treatment for cervical precancerous lesions.

scholarly journals Expression of Angiopoietin and VEGF in cervical cancer and its clinical significance

2018 ◽  
Vol 13 (1) ◽  
pp. 527-532
Author(s):  
Qingyuan Lv ◽  
Weijuan Zhong ◽  
Xiabin Ye ◽  
Yang Lv ◽  
Haiying Liu ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Clinical Significance ◽  
Squamous Cell ◽  
Tumor Tissue ◽  
Neoadjuvant Chemoradiotherapy ◽  
Lymphatic Vessels ◽  
Cervical Squamous Cell Carcinoma ◽  
Positive Expression

AbstractObjectiveThe aim of this study was to evaluate the expression of Angiopoietin-1 (Ang-1), Angiopoietin-2 (Ang-2) and vascular endothelial growth factor (VEGF) in cervical cancer and its clinical significance.MethodsImmunohistochemical assay was used to examine the expression of Ang-1/2 and VEGF in tumor tissue from 56 cervical squamous cell carcinoma patients treated with operation only (SCC-O group), as well as 51 subjects with cervical squamous cell carcinoma treated with neoadjuvant radiotherapy (SCC-RCO group, n=28) or neoadjuvant chemotherapy (SCC-CO group, n=23). Both microvessel density (MVD) and lymphatic vessel density (LVD) were examined in the three groups through detection of CD34 and D2-40 expression in respective tissue samples.ResultsWith the progression of cervical cancer, the positive expression scores of Ang-2 and VEGF were significantly increased (p<0.05). Compared with surgical intervention, neoadjuvant chemoradiotherapy significantly reduced the positive expression scores of Ang-1, Ang-2, and VEGF in cervical cancer tissues (p<0.05). The MVD values of the SCC-CO and SCC-RO groups were significantly reduced as compared to the SCC-O group (p<0.05). Similarly, the LVD values of the SCC-CO and SCC-RO groups were also significantly reduced when compared to those of the SCC-O group (p<0.05). However, LVD values of the SCC-CO and SCC-RO groups were not statistical different (p>0.05).ConclusionAng-1, Ang-2 and VEGF may play an important role in the development of cervical cancer. Mutual synergism of Ang-2 and VEGF demonstrated a close relationship with the generation of cervical blood and lymphatic vessels. Cervical cancer radiotherapy and chemotherapy could significantly inhibit the formation of blood vessels and lymphatic vessels in tumor tissue.

State of HPV16 integration in Lithuanian women with cervical neoplasia

Open Medicine ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. 205-212
Author(s):  
Agne Sepetiene ◽  
Zivile Gudlevicienė ◽  
Zana Bumbuliene ◽  
Grazina Drasutiene ◽  
Janina Didziapetriene
Keyword(s):  
Cervical Cancer ◽  
Hpv Infection ◽  
Rapid Progression ◽  
The European Union ◽  
Cervical Lesions ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Cin Iii ◽  
Cell Genome ◽  
Virus Integration

AbstractCervical cancer morbidity and mortality in Lithuania is one of the biggest in the European Union. The main risk factor of cervical cancer is human papillomavirus (HPV). The deletion of the HPV E2 gene influences HPV DNA integration into the cell genome, as well as a rapid progression of cervical lesions. The purpose of this study is to determine HPV, its types, and HPV 16 integration in different grades of cervical intraepithelial neoplasias (CIN). 253 women with cytological lesions were involved in the study. After a histology, 31 women were diagnosed with CIN I, 35 with CIN II, and 51 with CIN III. The biggest prevalence of HPV infection was detected in women younger than 25 years old (69.7%) and in women with CIN II (90.9%). HPV 16 was detected in 67.8% of all cases, with the highest prevalence in CIN III (84.4%). A partial integration form was detected in 65.0% of HPV 16 infected women, a complete virus integration in 26.5%, and an episomal form in 8.4% of cases. Our study concludes that in all the cases confirmed using a histology, the partial virus integration form of CIN was identified the most. It was less frequently detected in CIN I cases (60.0%), but more frequently in CIN II and CIN III cases (72.8 and 69.3%, respectively).

scholarly journals Significance of the Viral Load of High-risk Hpv in the Diagnosis and Prediction of Cervical Lesions: A Retrospective Study

2020 ◽  
Author(s):  
Yang Liu ◽  
Changjun Xu ◽  
Jing Pan ◽  
Chunyi Sun ◽  
Honglin Zhou
Keyword(s):  
Cervical Cancer ◽  
Viral Load ◽  
Retrospective Study ◽  
High Risk ◽  
High Viral Load ◽  
Control Group ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Cin Iii ◽  
Hpv Viral Load

Abstract Background: The significance of HPV viral load in the detection of cervical lesions is still controversial. This study analyzed the correlation between the high-risk (HR)-HPV viral load and different cervical lesion degrees.Methods: This was a retrospective study of the patients who first visited the hospital between January 2015 and June 2018. Patients with positive HR-HPV were screening for cervical cancer. The HR-HPV DNA load was measured by the second generation hybrid capture (HC2) technology. The patients grouped as normal, CIN I, CIN II, CIN III, and cervical cancer. Multivariable logistic regression was performed to explore the association between HR-HPV DNA load and cervical lesions.Results: Finally, 265 patients were grouped as normal (n=125), CIN I (n=51), CIN II (n=23), CIN III (n=46), and cervical cancer (n=20). Among them, 139 (52.5%) had a low viral load, 90 (34.0) had a moderate viral load, and 36 (13.4%) had a high viral load. Taking the normal control group as a reference, a high viral load was an independent factor for CIN I (CIN I: OR=3.959, 95%CI: 1.300-12.059, P=0.015) CIN II (OR=6.211, 95%CI: 1.641-23.513, P=0.007), CIN III (OR=7.002, 95%CI: 2.308-21.244, P=0.001), and cervical cancer (OR=9.439, 95%CI: 2.394-37.22, P=0.001).Conclusion: Cervical lesions are closely related to HR-HPV infection. Higher HR-HPV viral load in cervical lesions was associated with a higher risk of high-grade cervical lesions.

scholarly journals ThinPrep cytology combined with HPV detection in the diagnosis of cervical lesions in 1622 patients

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260915
Author(s):  
Sulaiya Husaiyin ◽  
Zhen Jiao ◽  
Kailibinuer Yimamu ◽  
Reyilanmu Maisaidi ◽  
Lili Han ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Precancerous Lesions ◽  
Screening Method ◽  
Diagnostic Value ◽  
Youden Index ◽  
Hpv Testing ◽  
Combined Method ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Diagnostic Values

The timely detection of precancerous lesions and early intervention can greatly reduce cervical cancer occurrence. The current study aimed to assess the diagnostic value and accuracy of different methods of cervical lesion screening. A total of 1622 females who visited the Outpatient Department of Xinjiang Uyghur Autonomous Region People’s Hospital between January and December 2018 were consecutively enrolled. All participants underwent separate high-risk human papilloma virus (HR-HPV) DNA detection, ThinPrep cytology testing (TCT) and colposcopic biopsy. Their medical records were retrospectively analyzed. While considering biopsy outcomes as the gold standard, the diagnostic values of TCT, HR-HPV testing, and TCT+HR-HPV testing for cervical cancer screening were compared. The sensitivity, specificity and Youden index of each method were calculated. Among the different methods, TCT+HR-HPV testing had the highest sensitivity (89.8%), followed by TCT (79.9%) and HR-HPV testing (49.2%). The combined method also had the highest Youden value, and its screening outcomes exhibited the highest consistency with those of biopsy. In addition, the combined method had the largest area under the receiver operating characteristic (ROC) curve, which was 0.673 (0.647, 0.699), compared with any other screening method. Compared with TCT or HR-HPV testing alone, TCT+HR-HPV testing serves as a better screening method for cervical cancer and precancerous lesions.

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