scholarly journals Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro

2020 ◽  
Vol 22 (1) ◽  
pp. 39
Author(s):  
Amalina Wahyuni ◽  
Benni Satria ◽  
Aprizal Zainal
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Economic Value ◽  
Market Demand ◽  
Compact Structure ◽  
Randomized Design ◽  
Aquilaria Malaccensis ◽  
Completely Randomized Design ◽  
Range Test

<p>Agarwood has a high economic value because they have many benefits that cause agarwood to be hunted to meet the increasing market demand and its presence in nature is decreasing due to unwise conventional retrieval systems.The research objectives were to study the effect and determine the best concentration of NAA and BAP in callus induction of agarwood (Aquilaria malaccensis Lamk.). This research was conducted in the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University from October 2018 until January 2019. The leaves of Agarwood were used as explants. The method used was completely randomized design (CRD) with different combinations of NAA and BAP (1.5 + 0.5 ppm, 3.0 + 0.5 ppm, 1.5 + 1.0 ppm, and 3.0 + 1.0 ppm). Data were analyzed using the F test and continued with Duncan’s Multiple Range Test at a level of 5%. The results showed that there was the effects of combination of growth regulator substances NAA and BAP on callus induction of agarwood. The concentration of NAA 3.0 ppm + BAP 0.5 ppm was the best in inducing callus with the percentage of explants life and explants form callus with 100%. The color of callus obtained varies, yellow, brownish yellow and brown. All structures callus gained in this research was compact.  Yellow callus and compact structure could be utilized for organogenesis.</p>

scholarly journals Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro

Jurnal BIOMA ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 34
Author(s):  
Febrina Ariyanti ◽  
Christiani Tumilisar ◽  
Rossa Yunita
Keyword(s):  
Tissue Culture ◽  
Economic Value ◽  
Shoot Elongation ◽  
Anacardium Occidentale ◽  
Culture Techniques ◽  
Randomized Design ◽  
Alternative Techniques ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

scholarly journals Pertumbuhan Tanaman Nanas pada Berbagai Konsentrasi IBA Secara In Vitro

2014 ◽  
Vol 4 (2) ◽  
pp. 287-292
Author(s):  
Zarmiyeni Zarmiyeni ◽  
Siti Munawarah Munawarah
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Butyric Acid ◽  
Market Demand ◽  
Indole Butyric Acid ◽  
The People ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
A New Technique

       The people of Hulu Sungai Utara Regency are very fond of pineapple, this can be seen from the continued increase in market demand for pineapple. Pineapple production in Hulu Sungai Utara Regency from 2010-2013 continues to increase. However, the availability of pineapple is still widely supplied from other regions. This is due to limited land and the availability of superior seeds. Therefore we need a new technique to overcome this problem by multiplying it in vitro. This study aims (i) to determine the response of pineapple plant growth to various concentrations of IBA (Indole Butyric Acid) in vitro and (ii) to obtain the best IBA (Indole Butyric Acid) concentration for pineapple plant growth in vitro. The study was conducted in the Amuntai STIPER tissue culture laboratory from March to June 2014, using a Completely Randomized Design (CRD) with 5 treatment levels, 4 replications. The factor tested was the IBA concentration, i1 = 1.5 ppm, i2 = 2 ppm, i3 = 2.5 ppm, i4 = 3 ppm, and i5 = 3.5 ppm. The results showed no known response and the best concentration of IBA on pineapple plant growth.

scholarly journals INDUCTION OF ABACA BANANA ROOTS BY IN VITRO USING KINDS OF MEDIA AND THIAMIN

Agrivet ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 1
Author(s):  
Rina Srilestari ◽  
Suwardi Suwardi
Keyword(s):  
Economic Value ◽  
Dry Weight ◽  
Root Induction ◽  
Market Demand ◽  
Fresh Weight ◽  
Skoog Medium ◽  
Randomized Design ◽  
Murashige And Skoog Medium ◽  
Completely Randomized Design

Abaca is a type a banana with high economic value with it stem fiber used in textile and paper industries. As a superior commodity, its number is relatively limited, with the need of a largeplanting area to meet the high market demand. The aim of the research was to observe the abaca banana explants response to various media and Thiamin. The experiment was done at Biotechnology laboratory, UPN “Veteran” Yogyakarta. Treatments were arranged in completely randomized design with 2 factor. The first factor is the growing media: Murashige & Skoog, a half Murashige & Skoog media, Vacint & Went Media and the second factor is the Thiamin concentration: 2 mg/L; 3 mg/L; 4 mg/mL.The results showed there is an interactions on the parameters of planlet height, number of lenghth of root in the combination of Murashige and Skoog and thiamin 3 mg/L medium. Murashige and Skoog medium produced the highest fresh weight and dry weight and Thiamin concentration 3 mg/L produced fresh and dry weight in the highestKey words: abaka, root induction, various media, thiamin

scholarly journals Penggunaan Bahan Organik dan Kombinasinya dalam Formulasi Biofungisida Berbahan Aktif Jamur Trichoderma pseudokoningii Rifai. untuk Menghambat Jamur Ganoderma boninense Pat. secara in vitro

2017 ◽  
Vol 16 (2) ◽  
pp. 79
Author(s):  
Yetti Elfina ◽  
Muhammad Ali ◽  
Rachmad Saputra
Keyword(s):  
Rice Husk ◽  
Ganoderma Boninense ◽  
Organic Matters ◽  
Randomized Design ◽  
Shrimp Shell ◽  
Trichoderma Pseudokoningii ◽  
Completely Randomized Design ◽  
Rot Disease ◽  
Range Test

Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.

scholarly journals Seleksi Toleransi Kekeringan In Vitro terhadap Enam Belas Aksesi Tanaman Terung (Solanum melongena L. ) dengan Polietilena Glikol (PEG)

2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya
Keyword(s):  
Tissue Culture ◽  
Drought Tolerance ◽  
In Vitro Selection ◽  
Solanum Melongena ◽  
Survival Percentage ◽  
Drought Tolerant ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>

scholarly journals THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 39
Author(s):  
Salma Nabila ◽  
Endah Budi Irawati ◽  
Rina Srilestari
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety  of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on  fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

scholarly journals Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations

Akta Agrosia ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 29-35
Author(s):  
Usman Kris Joko Suharjo ◽  
Hasanudin Hasanudin ◽  
Tunjung Pamekas ◽  
Hesti Pujiwati ◽  
Alyi Vanturini
Keyword(s):  
Poor Quality ◽  
Tuber Formation ◽  
Potato Tubers ◽  
Seed Sources ◽  
Randomized Design ◽  
Plant Culture ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Range Test

A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The  research  design  used  was  Completely  Randomized  Design  (CRD)  which  consist  of  18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and  Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato

scholarly journals Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. LUKMAN ◽  
. AGUS-PURWITO
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Randomized Design ◽  
Top Soil ◽  
Completely Randomized Design ◽  
Reduced Light ◽  
In Vitro Micrografting ◽  
Cinchona Ledgeriana

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization  for Cinchona plantlets from  micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of  C. ledgeriana  QRC clone were used as  scions, while of C. succirubra as  rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of  combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were  the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived  plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization.  Acclimatization  consisted  of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC  for two weeks,  followed by aclimatization in a plastic house with  70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan  Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan.  Pada tanaman kina teknik sambung mikro  in vitro belum pernah dilakukan. Tujuan penelitian ini adalah  menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung  mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi  medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet  C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu  kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan  dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup,  jumlah daun,  berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung  mikro  yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro.  Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur  suhu 25 -     27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini  90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal   Cinchona sp..

scholarly journals Growth Induction of Cavendish Buds (Musa Acuminata L.) on Difference Concentration of IBA and BA in Vitro

2019 ◽  
Vol 8 (2) ◽  
pp. 178-187
Author(s):  
Subandi M ◽  
Arkhan Jannata ◽  
Sofiya Hasani
Keyword(s):  
Musa Acuminata ◽  
Laboratory Research ◽  
Independent Effect ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growth Induction

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

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