scholarly journals Effects of Temperature and Nutrient Conditions on Biofilm Formation of Pseudomonas putida

2012 ◽  
Vol 18 (6) ◽  
pp. 879-883 ◽  
Author(s):  
Kazuya MORIMATSU ◽  
Kodai EGUCHI ◽  
Daisuke HAMANAKA ◽  
Fumihiko TANAKA ◽  
Toshitaka UCHINO
Keyword(s):  
Pseudomonas Putida ◽  
Biofilm Formation ◽  
Effects Of Temperature ◽  
Nutrient Conditions

scholarly journals MqsR/MqsA Toxin/Antitoxin System Regulates Persistence and Biofilm Formation in Pseudomonas putida KT2440

2017 ◽  
Vol 8 ◽  
Author(s):  
Chenglong Sun ◽  
Yunxue Guo ◽  
Kaihao Tang ◽  
Zhongling Wen ◽  
Baiyuan Li ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Biofilm Formation ◽  
Pseudomonas Putida Kt2440

scholarly journals Dechlorination of Chloral Hydrate Is Influenced by the Biofilm Adhesin Protein LapA in Pseudomonas putida LF54

2013 ◽  
Vol 79 (13) ◽  
pp. 4166-4169
Author(s):  
Wanjun Zhang ◽  
Huhe ◽  
Yuanbai Pan ◽  
Masanori Toyofuku ◽  
Nobuhiko Nomura ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Biofilm Formation ◽  
Chloral Hydrate ◽  
Insertion Mutagenesis ◽  
Surface Adhesion ◽  
Content Type ◽  
Adhesion Protein ◽  
Transposon Insertion ◽  
Adhesin Protein ◽  
First Time

ABSTRACTLapA is the largest surface adhesion protein ofPseudomonas putidathat initiates biofilm formation. Here, by using transposon insertion mutagenesis and a conditionallapAmutant, we demonstrate for the first time that LapA influences chloral hydrate (CH) dechlorination inP. putidaLF54.

scholarly journals Cellular L-arginine pools modulate c-di-GMP turnover and biofilm formation in Pseudomonas putida

2020 ◽  
Author(s):  
Laura Barrientos-Moreno ◽  
María Antonia Molina-Henares ◽  
María Isabel Ramos-González ◽  
Manuel Espinosa-Urgel
Keyword(s):  
Pseudomonas Putida ◽  
Biofilm Formation ◽  
Response Regulator ◽  
Second Messenger ◽  
Colony Morphology ◽  
Diguanylate Cyclase ◽  
Arginine Biosynthesis ◽  
Amino Acid Transport System ◽  
Metabolic Signal ◽  
Biofilm Development

<p>The intracellular second messenger cyclic diguanylate (c-di-GMP) is broadly conserved in bacteria, where it influences processes such as virulence, stress resistance and biofilm development. In the plant-beneficial bacterium <em>Pseudomonas putida</em> KT2440, the response regulator with diguanylate cyclase activity CfcR is the main contributor to c-di-GMP levels in the stationary phase of growth. When overexpressed, CfcR increases c-di-GMP levels and gives rise to a pleiotropic phenotype that includes enhanced biofilm formation and crinkly colony morphology. Our group has previously reported that insertion mutants in <em>argG</em> and <em>argH</em>, the genes that encode the last two enzymes in the arginine biosynthesis pathway, do not display the crinkly colony morphology phenotype and show decreased c-di-GMP levels even in the presence of <em>cfcR</em> in multicopy (Ramos-González, M.I. <em>et al.</em> 2016. Front. Microbiol. 7, 1093). Here we present results indicating that L-arginine acts both as an environmental and as a metabolic signal that influences the lifestyles of <em>P. putida</em> through the modulation of c-di-GMP levels and changes in the expression of structural elements of biofilms. Exogenous L-arginine partially restores c-di-GMP levels in arginine biosynthesis mutants, a response that is transduced through CfcR and possibly (an)other diguanylate cyclase(s). At least three periplasmic binding proteins, each forming part of an amino acid transport system, contribute in different ways to the response to external L-arginine. We propose that the turnover of the second messenger c-di-GMP is modulated by the state of global arginine pools in the cell resulting both from anabolism and from uptake.</p>

scholarly journals Pseudomonas Putida as a Potential Biocontrol Agent Against Salmonella Java Biofilm Formation in the Drinking Water System of Broiler Houses

2020 ◽  
Author(s):  
Sharon Maes ◽  
Koen De Reu ◽  
Stephanie Van Weyenberg ◽  
Bram Lories ◽  
Marc Heyndrickx ◽  
...  
Keyword(s):  
Drinking Water ◽  
Pseudomonas Putida ◽  
Biofilm Formation ◽  
Broiler Chickens ◽  
Water System ◽  
Inoculum Density ◽  
Microtiter Plates ◽  
Drinking Water System ◽  
First Results

Abstract Background: Environmental biofilms can induce attachment and protection of other microorganisms including pathogens, but can also prevent them from invasion and colonization. This opens the possibility for so-called biocontrol strategies, wherein microorganisms are applied to control the presence of other microbes. The potential for both positive and negative interactions between microbes, however, raises the need for in depth characterization of the sociobiology of candidate biocontrol agents (BCAs). The inside of the drinking water system (DWS) of broiler houses is an interesting niche to apply BCAs, because contamination of these systems with pathogens plays an important role in the infection of broiler chickens and consequently humans. In this study, Pseudomonas putida, which is part of the natural microbiota in the DWS of broiler houses, was evaluated as BCA against the broiler pathogen Salmonella Java.Methods: To study the interaction between these species, an in vitro model was developed simulating biofilm formation in the drinking water system of broilers. Mono- and dual-species biofilms were grown in 6-well microtiter plates and quantified based on bacterial counts and social interactions in mixed communities were determined by the cooperation criterion and biodiversity effect.Results: Dual-species biofilms of P. putida strains P1, P2, and P3 with S. Java were characterized by competitive interactions, independent of P. putida strain, S. Java inoculum density and application order. When equal inocula of S. Java and P. putida strains P1 or P3 were simultaneously applied, the interaction was characterized by mutual inhibition, whereas P. putida strain P2 showed an exploitation of S. Java. Lowering the inoculum density of S. Java changed the interaction with P. putida strain P3 also into an exploitation of S. Java. A further increase in S. Java inhibition was established by P. putida strain P3 forming a mature biofilm before applying S. Java.Conclusions: This study provides the first results showing the potential of P. putida as BCA against S. Java in the broiler environment. Future work should include more complex microbial communities residing in the DWS, additional Salmonella strains as well as chemicals typically used to clean and disinfect the system.

Biofilm formation of Pseudomonas putida IsoF: the role of quorum sensing as assessed by proteomics

2005 ◽  
Vol 28 (2) ◽  
pp. 87-114 ◽  
Author(s):  
Catalina Arevalo-Ferro ◽  
Gerold Reil ◽  
Angelika Görg ◽  
Leo Eberl ◽  
Kathrin Riedel
Keyword(s):  
Quorum Sensing ◽  
Pseudomonas Putida ◽  
Biofilm Formation

scholarly journals Self-Regulation and Interplay of Rsm Family Proteins Modulate the Lifestyle of Pseudomonas putida

2016 ◽  
Vol 82 (18) ◽  
pp. 5673-5686 ◽  
Author(s):  
Óscar Huertas-Rosales ◽  
María Isabel Ramos-González ◽  
Manuel Espinosa-Urgel
Keyword(s):  
Pseudomonas Putida ◽  
Biofilm Formation ◽  
Expression Patterns ◽  
Self Regulation ◽  
Bacterial Attachment ◽  
Triple Mutant ◽  
Content Type ◽  
Global Regulators ◽  
Glass Surfaces ◽  
Plastic Surfaces

ABSTRACTIn the plant-beneficial bacteriumPseudomonas putidaKT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles ofP. putidahave been investigated by generating single-, double-, and triple-null mutants and by overexpressing each protein (RsmA, RsmE, and RsmI) in different genetic backgrounds. Thersmtriple mutant shows reduced swimming and swarming motilities and increased biofilm formation, whereas overexpression of RsmE or RsmI results in reduced bacterial attachment. However, biofilms formed on glass surfaces by the triple mutant are more labile than those of the wild-type strain and are easily detached from the surface, a phenomenon that is not observed on plastic surfaces. Analysis of the expression of adhesins and exopolysaccharides in the different genetic backgrounds suggests that the biofilm phenotypes are due to alterations in the composition of the extracellular matrix and in the timing of synthesis of its elements. We have also studied the expression patterns of Rsm proteins and obtained data that indicate the existence of autoregulation mechanisms.IMPORTANCEProteins of the CsrA/RsmA family function as global regulators in different bacteria. More than one of these proteins is present in certain species. In this study, all of the RsmA homologs inP. putidaare characterized and globally taken into account to investigate their roles in controlling bacterial lifestyles and the regulatory interactions among them. The results offer new perspectives on how biofilm formation is modulated in this environmentally relevant bacterium.

scholarly journals Membrane Vesicle Formation as a Multiple-Stress Response Mechanism Enhances Pseudomonas putida DOT-T1E Cell Surface Hydrophobicity and Biofilm Formation

2012 ◽  
Vol 78 (17) ◽  
pp. 6217-6224 ◽  
Author(s):  
Thomas Baumgarten ◽  
Stefanie Sperling ◽  
Jana Seifert ◽  
Martin von Bergen ◽  
Frank Steiniger ◽  
...  
Keyword(s):  
Stress Response ◽  
Cell Surface ◽  
Pseudomonas Putida ◽  
Biofilm Formation ◽  
Membrane Vesicle ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Outer Membrane Vesicles ◽  
Stress Factors ◽  
Content Type

ABSTRACTAmong the adaptive responses of bacteria to rapid changes in environmental conditions, those of the cell envelope are known to be the most crucial. Therefore, several mechanisms with which bacteria change their cell surface and membranes in the presence of different environmental stresses have been elucidated. Among these mechanisms, the release of outer membrane vesicles (MV) in Gram-negative bacteria has attracted particular research interest because of its involvement in pathogenic processes, such as that ofPseudomonas aeruginosabiofilm formation in cystic fibrosis lungs. In this study, we investigated the role of MV formation as an adaptive response ofPseudomonas putidaDOT-T1E to several environmental stress factors and correlated it to the formation of biofilms. In the presence of toxic concentrations of long-chain alcohols, under osmotic stress caused by NaCl, in the presence of EDTA, and after heat shock, cells of this strain released MV within 10 min in the presence of a stressor. The MV formed showed similar size and charge properties, as well as comparable compositions of proteins and fatty acids. MV release caused a significant increase in cell surface hydrophobicity, and an enhanced tendency to form biofilms was demonstrated in this study. Therefore, the release of MV as a stress response could be put in a physiological context.

Biofilm Development and Sanitizer Inactivation of Listeria monocytogenes and Salmonella typhimurium on Stainless Steel and Buna-n Rubber

1993 ◽  
Vol 56 (9) ◽  
pp. 750-758 ◽  
Author(s):  
AMY B. RONNER ◽  
AMY C. L. WONG
Keyword(s):  
Stainless Steel ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Biofilm Formation ◽  
Bacterial Biofilm ◽  
Extracellular Matrices ◽  
Nutrient Level ◽  
Extracellular Materials ◽  
Biofilm Development ◽  
Nutrient Conditions

Biofilm formation by seven strains of Listeria monocytogenes and one strain of Salmonella typhimurium on stainless steel and Buna-n rubber was examined under two nutrient conditions. The type of surface, nutrient level, and organism influenced biofilm development and production of extracellular materials. Buna-n had a strong bacteriostatic effect on L. monocytogenes, and biofilm formation on Buna-n under low nutrient conditions was reduced for four of the seven strains tested. Buna-n was less bacteriostatic toward S. typhimurium. It inhibited the growth of several other pathogens to varying degrees. An ethylene propylene diamine monomer rubber was less inhibitory than Buna-n, and Viton rubber had no effect. The effectiveness of sanitizers on biofilm bacteria was examined. Biofilms were challenged with four types of detergent and nondetergent sanitizers. Resistance to sanitizers was strongly influenced by the type of surface. Bacterial biofilm populations on stainless steel were reduced 3–5 log by all the sanitizers, but those on Buna-n were resistant to these sanitizers and were reduced less than 1–2 log. In contrast, planktonic (suspended) bacteria were reduced 7–8 log by these sanitizers. Chlorine and anionic acid sanitizers generally removed extracellular materials from biofilms better than iodine and quaternary ammonium detergent sanitizers. Scanning electron microscopy demonstrated that biofilm cells and extracellular matrices could remain on sanitized biofilm cells and extracellular matrices could remain surfaces from which no viable cells were recovered.

scholarly journals Pseudomonas putida as a potential biocontrol agent against Salmonella Java biofilm formation in the drinking water system of broiler houses

2020 ◽  
Author(s):  
Sharon Maes ◽  
Koen De Reu ◽  
Stephanie Van Weyenberg ◽  
Bram Lories ◽  
Marc Heyndrickx ◽  
...  
Keyword(s):  
Drinking Water ◽  
Pseudomonas Putida ◽  
Biofilm Formation ◽  
Broiler Chickens ◽  
Water System ◽  
Inoculum Density ◽  
Microtiter Plates ◽  
Drinking Water System ◽  
First Results

Abstract Background: Environmental biofilms can induce attachment and protection of other microorganisms including pathogens, but can also prevent them from invasion and colonization. This opens the possibility for so-called biocontrol strategies, wherein microorganisms are applied to control the presence of other microbes. The potential for both positive and negative interactions between microbes, however, raises the need for in depth characterization of the sociobiology of candidate biocontrol agents (BCAs). The inside of the drinking water system (DWS) of broiler houses is an interesting niche to apply BCAs, because contamination of these systems with pathogens plays an important role in the infection of broiler chickens and consequently humans. In this study, Pseudomonas putida, which is part of the natural microbiota in the DWS of broiler houses, was evaluated as BCA against the broiler pathogen Salmonella Java.Methods: To study the interaction between these species, an in vitro model was developed simulating biofilm formation in the drinking water system of broilers. Mono- and dual-species biofilms were grown in 6-well microtiter plates and quantified based on bacterial counts and social interactions in mixed communities were determined by the cooperation criterion and biodiversity effect.Results: Dual-species biofilms of P. putida strains P1, P2, and P3 with S. Java were characterized by competitive interactions, independent of P. putida strain, S. Java inoculum density and application order. When equal inocula of S. Java and P. putida strains P1 or P3 were simultaneously applied, the interaction was characterized by mutual inhibition, whereas P. putida strain P2 showed an exploitation of S. Java. Lowering the inoculum density of S. Java changed the interaction with P. putida strain P3 also into an exploitation of S. Java. A further increase in S. Java inhibition was established by P. putida strain P3 forming a mature biofilm before applying S. Java.Conclusions: This study provides the first results showing the potential of P. putida as BCA against S. Java in the broiler environment. Future work should include more complex microbial communities residing in the DWS, additional Salmonella strains as well as chemicals typically used to clean and disinfect the system.

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