Anti-Adipogenic Activity of Berberine in 3T3-L1 Pre-Adipocytes Cultured in High-Glucose Medium

Type 2 diabetes mellitus (T2DM) is considered to compromise implant osseointegration. Protein tyrosine phosphatase non-receptor type 2 (PTPN2) regulates glucose metabolism, systemic inflammation, and bone regeneration. This study aimed to investigate the role of PTPN2 in implant osseointegration in T2DM and explore the potential mechanisms. Streptozotocin-induced diabetic rats received implant surgery, with or without local overexpression of PTPN2 for three months, and implant osseointegration was examined by histological evaluation, micro-CT analysis, pull-out test, and scanning electron microscope. Rat bone marrow stem cells (RBMSCs) were isolated and exposed to high glucose, and osteogenic differentiation was evaluated by alizarin red staining, ALP assay, and Western blot analysis. Overexpression of PTPN2 could improve impaired implant osseointegration in T2DM rats and promote osteogenic differentiation of RBMSCs in high glucose. In addition, p-ERK level in RBMSCs was increased in high glucose and decreased after PTPN2 overexpression. These results suggest that PTPN2 promotes implant osseointegration in T2DM rats and enhances osteogenesis of RBMSCs in high glucose medium via inducing the dephosphorylation of ERK. PTPN2 may be a novel target for the therapy of impaired implant osseointegration in T2DM patients. Impact statement Using both in vivo and in vitro approaches, we made important findings that PTPN2 promoted implant osseointegration in T2DM rats and enhanced osteogenesis of RBMSCs in high glucose medium. The positive effects of PTPN2 on osteogenesis are related to the dephosphorylation of ERK and the inhibition of MAPK/ERK pathway. PTPN2 may be a novel target for the therapy of impaired implant osseointegration in T2DM patients.

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Nutrient Utilization by Bovine Articular Chondrocytes: A Combined Experimental and Theoretical Approach

Journal of Biomechanical Engineering ◽

10.1115/1.1993664 ◽

2005 ◽

Vol 127 (5) ◽

pp. 758-766 ◽

Cited By ~ 45

Author(s):

Bram G. Sengers ◽

Hannah K. Heywood ◽

David A. Lee ◽

Cees W. J. Oomens ◽

Dan L. Bader

Keyword(s):

Oxygen Uptake ◽

High Glucose ◽

Articular Chondrocytes ◽

Lactate Production ◽

Glucose Medium ◽

Numerical Predictions ◽

High Glucose Medium ◽

Biosensor System ◽

Wide Range ◽

Bovine Articular Chondrocytes

A combined experimental-numerical approach was adopted to characterize glucose and oxygen uptake and lactate production by bovine articular chondrocytes in a model system. For a wide range of cell concentrations, cells in agarose were supplemented with either low or high glucose medium. During an initial culture phase of 48h, oxygen was monitored noninvasively using a biosensor system. Glucose and lactate were determined by medium sampling. In order to quantify glucose and oxygen uptake, a finite element approach was adopted to describe diffusion and uptake in the experimental model. Numerical predictions of lactate, based on simple relations for cell metabolism, were found to agree well for low glucose, but not for high glucose medium. Oxygen did not play a role in either case. Given the close association between chondrocyte energy metabolism and matrix synthesis, a quantifiable prediction of utilization can present a valuable contribution in the optimization of tissue engineering conditions.

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Effect of Jinmaitong (筋脉通) serum on the proliferation of rat Schwann cells cultured in high glucose medium

Chinese Journal of Integrative Medicine ◽

10.1007/s11655-008-0293-z ◽

2008 ◽

Vol 14 (4) ◽

pp. 293-297 ◽

Cited By ~ 12

Author(s):

Ling Qu ◽

Xiao-chun Liang ◽

Hong Zhang ◽

Qun-li Wu ◽

Lian-qing Sun ◽

...

Keyword(s):

Schwann Cells ◽

High Glucose ◽

Glucose Medium ◽

High Glucose Medium ◽

Cells Cultured

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Increased endocytosis in retinal vascular endothelial cells grown in high glucose medium is modulated by inhibitors of nonenzymatic glycosylation

Diabetologia ◽

10.1007/s001250050423 ◽

1995 ◽

Vol 38 (11) ◽

pp. 1271-1275 ◽

Cited By ~ 1

Author(s):

A. W. Stitt ◽

U. Chakravarthy ◽

D. B. Archer ◽

T. A. Gardiner

Keyword(s):

Endothelial Cells ◽

High Glucose ◽

Vascular Endothelial Cells ◽

Vascular Endothelial ◽

Glucose Medium ◽

Nonenzymatic Glycosylation ◽

High Glucose Medium

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CircRNA-DAPK1 promotes vascular cell pyroptosis in diabetes by regulating the circ-DAPK1/miR-4454/TXNIP axis.

10.21203/rs.3.rs-218972/v1 ◽

2021 ◽

Author(s):

Tianchi Chen ◽

Xiangtao Zheng ◽

Yangyan He ◽

Chenyang Qiu ◽

Xiaohui Wang ◽

...

Keyword(s):

High Glucose ◽

Luciferase Reporter ◽

Glucose Medium ◽

Vascular Cell ◽

Normal Medium ◽

High Glucose Medium ◽

Signaling Axis

Abstract Background Circular RNAs have been demonstrated to play an important role in the development of vascular diseases. However, little is known about the role of circ-021774, also named circ-DAPK1, in vascular cell pyroptosis. Methods Circ-DAPK1 was selected from circular RNA sequencing data of HUVECs treated with high glucose medium and normal medium. RT-qPCR was used to determine the expression of circ-DAPK1 in vivo and in vitro. Dual luciferase reporter assay, fluorescence in situ hybridization (FISH) and RNA immunoprecipitation (RIP) were performed to prove the interaction of circ-DAPK1, miRNA-4454 and thioredoxin-interactingprotein (TXNIP). Adeno-associated virus (AAV) was injected intravenously to establish mouse models. PI staining, western-blot and transmission electron microscopy (TEM) analyses were performed to identify the role of circ-DAPK1 in promoting pyroptosis. Results We found that circ-DAPK1 was highly expressed in high glucose medium cultured HUVECs and db/db mice. In vitro and in vivo experiments demonstrated that circ-DAPK1 knockdown decreased the number of PI+ cells, the expression of ASC, NLRP3, GSDMD-N, cleaved caspase-1, IL-18 and IL-1β. In a mechanistic study, the circ-DAPK1/miRNA-4454/TXNIP signaling axis was demonstrated to promote vascular cell pyroptosis in diabetes. Conclusions Circ-DAPK1 functions as a promoter of vascular cell pyroptosis in diabetes via the circ-DAPK1/miRNA-4454/TXNIP signaling axis.

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