scholarly journals Isolation of Candida species in children and their biofilm-forming ability on nano-composite surfaces

2020 ◽  
Vol 1 (1) ◽  
pp. 40-52
Author(s):  
Harina Akila Che Hussin ◽  
Nadiah Arshad ◽  
Siti Nor Humaira Nor Azemi ◽  
Raja Izzatun Nisa Raja Sahrul Hishan ◽  
Wan Nur Fatihah Wan Mohd Kamaluddin ◽  
...  
Keyword(s):  
Dental Caries ◽  
Oral Cavity ◽  
Buccal Mucosa ◽  
Candida Species ◽  
Soft Tissues ◽  
Tooth Surface ◽  
Nano Composites ◽  
Candida Spp ◽  
Nano Composite ◽  
Tooth Surfaces

Candida species including Candida albicans, Candida krusei and Candida glabrata are opportunistic microorganisms that inhabit oral cavity. The objective of this study is to determine the effect of dental caries on Candida spp. biofilm-forming ability on nano- composite with the hypothesis that dental caries enhances the colonization of Candida spp. To assess Candida spp. colonisation in the oral cavity of the paediatric patient, samples were obtained from 30 subjects aged five to six years old from Kuantan, Pahang, Malaysia. The samples were collected from buccal mucosa, palate and tooth surfaces using sterile swabs. 10 mL of patient’s saliva suspension was also collected. Following that, the samples were inoculated on CHROMagar and incubated for 24 h at 37 ºC. Candida biofilm of caries isolate C. albicans (HNFC2), and C. albicans ATCC 32354 were developed on three different types of nano-composites. The study showed that no C. albicans was isolated from the caries-free oral cavity while 76% of children with caries possessed Candida spp. 65% of the yeasts were isolated from the tooth surface. Only 35% of the total isolates were obtained from soft tissues, including palatal and buccal mucosa. C. albicans is the most isolated Candida spp. with 82% and 67% of the yeast were obtained from the tooth surface and buccal mucosa, respectively. Besides, HNFC2 significantly colonised the nano- composites more than the ATCC (P < 0.05). In the comparison of the three types of nano- composites, nano-hybrid-based containing pre-polymerised filler (cB) exhibited the least C. albicans HNFC2 cells colonisation with 7.7 x 10³ cells mL-1. In contrast, the nano-composite that contained bulk-filled nanohybrid (cC) was the most colonised with 14.3 x 10³ cells mL-1. In conclusion, dental caries enhances the colonization of Candida spp. in children's oral cavity, and that caries isolate form more biofilm on nano-composites compared to the lab strain C. albicans.

scholarly journals Candida spp. occurrence in oral cavities of breastfeeding infants and in their mothers' mouths and breasts

2003 ◽  
Vol 17 (2) ◽  
pp. 151-155 ◽  
Author(s):  
Maria Stella Amorim da Costa Zöllner ◽  
Antonio Olavo Cardoso Jorge
Keyword(s):  
Oral Cavity ◽  
Candida Species ◽  
Statistical Difference ◽  
Control Group ◽  
Biochemical Tests ◽  
Candida Spp ◽  
Lactating Women ◽  
Sabouraud Agar ◽  
Breastfed Infants ◽  
Yeast Strains

This study aimed to determine the occurrence of Candida spp. in the oral cavity of predominantly breastfed infants and in their mothers' mouths and breasts, as well as in the oral cavity of bottlefed infants and in non-lactating women. One hundred and sixty nine women and eighty-five milk-fed infants took part in this study and were divided into four groups: 1) infants predominantly on breastfeeding (n = 55) and their mothers (n = 55); 2) infants on bottlefeeding (n = 30); 3) non-lactating women on whom oral collections were performed (n = 80) and, 4) non-lactating women on whom breast collections were performed (n = 34). Oral and mammary swabs were cultured on Sabouraud agar dextrose with chloramphenicol. The Candida yeast strains found were isolated and identified through morphological and biochemical tests. Candida species were much less frequent in infants who were predominantly breastfed than in those who were bottlefed. Yeasts were much more frequent on the breasts of lactating women, with statistical difference in relation to the control group.

scholarly journals Cloning, Expression, Purification, and Preliminary Characterization of Single-Crystal X-Ray Diffraction of Glucosyltransferase B of Streptococcus mutans

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984933
Author(s):  
Joshua L. Mieher ◽  
Norbert Schormann ◽  
Manisha Patel ◽  
Hui Wu ◽  
Champion Deivanayagam
Keyword(s):  
Dental Caries ◽  
Oral Cavity ◽  
Single Crystal ◽  
Streptococcus Mutans ◽  
Tooth Enamel ◽  
Tooth Surface ◽  
X Ray Diffraction ◽  
Persistent Disease ◽  
X Ray

Dental caries characterized by acid damage of tooth enamel is a persistent disease that begins with the formation of biofilms on the tooth surface. The secreted glucosyltransferases enable Streptococcus mutans to synthesize extracellular glucan polymers using ingested starch within the oral cavity, which eventually results in the production of acid, a contributing factor to cariogenesis. In this paper, we report the cloning, expression, purification, crystallization, and preliminary X-ray diffraction characterization of glucosyltransferase B.

scholarly journals Murein Hydrolase LytF of Streptococcus sanguinis and the Ecological Consequences of Competence Development

2017 ◽  
Vol 83 (24) ◽  
Author(s):  
Nyssa Cullin ◽  
Sylvio Redanz ◽  
Kirsten J. Lampi ◽  
Justin Merritt ◽  
Jens Kreth
Keyword(s):  
Dental Caries ◽  
Oral Cavity ◽  
Cell Morphology ◽  
Biofilm Formation ◽  
Oral Bacteria ◽  
Tooth Surface ◽  
Content Type ◽  
Streptococcus Sanguinis ◽  
Murein Hydrolase ◽  
Biofilm Development

ABSTRACT The overall health of the oral cavity is dependent on proper homeostasis between health-associated bacterial colonizers and bacteria known to promote dental caries. Streptococcus sanguinis is a health-associated commensal organism, a known early colonizer of the acquired tooth pellicle, and is naturally competent. We have shown that LytF, a competence-controlled murein hydrolase, is capable of inducing the release of extracellular DNA (eDNA) from oral bacteria. Precipitated LytF and purified LytF were used as treatments against planktonic cultures and biofilms. Larger amounts of eDNA were released from cultures treated with protein samples containing LytF. Additionally, LytF could affect biofilm formation and cellular morphology. Biofilm formation was significantly decreased in the lytF-complemented strain, in which increased amounts of LytF are present. The same strain also exhibited cell morphology defects in both planktonic cultures and biofilms. Furthermore, the LytF cell morphology phenotype was reproducible in wild-type cells using purified LytF protein. In sum, our findings demonstrate that LytF can induce the release of eDNA from oral bacteria, and they suggest that, without proper regulation of LytF, cells display morphological abnormalities that contribute to biofilm malformation. In the context of the oral biofilm, LytF may play important roles as part of the competence and biofilm development programs, as well as increasing the availability of eDNA. IMPORTANCE Streptococcus sanguinis, a commensal organism in the oral cavity and one of the pioneer colonizers of the tooth surface, is associated with the overall health of the oral environment. Our laboratory showed previously that, under aerobic conditions, S. sanguinis can produce H2O2 to inhibit the growth of bacterial species that promote dental caries. This production of H2O2 by S. sanguinis also induces the release of eDNA, which is essential for proper biofilm formation. Under anaerobic conditions, S. sanguinis does not produce H2O2 but DNA is still released. Determining how S. sanguinis releases DNA is thus essential to understand biofilm formation in the oral cavity.

Diagnosis and prevention of dental caries

2018 ◽  
Author(s):  
C. Deery ◽  
K.J. Toumba
Keyword(s):  
Dental Caries ◽  
Dental Plaque ◽  
Enamel Surface ◽  
Tooth Surface ◽  
Surface Phenomenon ◽  
Carious Lesion ◽  
Tooth Surfaces ◽  
Carious Process ◽  
Intact Surface ◽  
Micro Organisms

Almost all research on the process of dental caries supports the chemoparasitic theory proposed by W.D. Miller in 1890. This is now more commonly known as the acidogenic theory of caries aetiology. The main features of the caries process are as follows. 1. Fermentation of carbohydrate to organic acids by micro-organisms in plaque on the tooth surface. 2. Acid production, which lowers the pH at the enamel surface below the level (the critical pH) at which enamel will dissolve. 3. When carbohydrate is no longer available to the plaque microorganisms, the pH within plaque will rise because of the outward diffusion of acids and their metabolism and neutralization in plaque, so that remineralization of enamel can occur; 4. Dental caries progresses only when the balance between demineralization and remineralization favours the former. The realization that demineralization and remineralization are in equilibrium is key to understanding the dynamics of the carious lesion and its prevention. One of the interesting features of an initial carious lesion of the enamel is that the lesion is subsurface, i.e. most of the mineral loss occurs beneath a relatively intact enamel surface. This contrasts strongly with the histological appearance of enamel after a clean tooth surface has been exposed to acid, where the surface is etched and there is no subsurface lesion. This dissolution of the surface of enamel, or etching, is a feature of enamel erosion caused, among other things, by dietary acids. Therefore the carious process and erosion differ completely, as erosion is a surface phenomenon. The explanation for the intact surface layer in enamel caries seems to lie in diffusion dynamics: the layer of dental plaque on the tooth surface acts as a partial barrier to diffusion. Further erosion occurs at much lower pH values (pH <4) than caries. Dental plaque forms on uncleaned tooth surfaces and is readily apparent if toothbrushing is stopped for 2–3 days. Contrary to popular opinion, plaque does not consist of food debris, but is a biofilm; 70% is comprised of micro-organisms—about 100 million organisms per milligram of plaque. When plaque is young cocci predominate, but as plaque ages the proportions of filamentous organisms and veillonellae increase.

scholarly journals Evaluation of the frequency of Candida spp. in hospitalized and non-hospitalized subjects

2018 ◽  
Vol 78 (4) ◽  
pp. 644-652 ◽  
Author(s):  
J. N. Vieira ◽  
A. M. Feijó ◽  
M. E. Bueno ◽  
C. L. Gonçalves ◽  
R. G. Lund ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Teaching Hospital ◽  
Candida Species ◽  
Hospitalized Patients ◽  
The Other ◽  
Medical Clinic ◽  
Candida Spp ◽  
Sabouraud Dextrose Agar ◽  
Peptone Water

Abstract The aim of this study was to evaluate the frequency of Candida species between a non-hospitalized and a hospitalized population. For this purpose, samples of saliva were sampled through sterile swabs, moistened in peptone water and rubbed in the oral cavity of 140 individuals, from which, 70 were hospitalized patients from the Medical Clinic of a Teaching Hospital and the other 70 were non-hospitalized subjects. All saliva samples were plated in Sabouraud Dextrose agar added with Chloramphenicol and incubated at 36 °C for 48 hours. The morphology identification was performed through macroscopic and microscopic characterization, the CHROMagar Candida medium and the VITEK® system Yeast Biochemical Card (bio Mérieux SA, France). The results showed a colonization of Candida spp. in 85.7% the hospitalized individuals, where the species found were C. albicans (60%), C. tropicalis (23.4%), C. krusei (3.3%) and Candida spp. (13.3%). In the non-hospitalized individuals the colonization by Candida spp was 47.1%, and the species found were: C. albicans (45.5%), C.krusei (9.1%), C. guilliermondii (9.1% %), C. tropicalis (3.0%), C. famata (3.0%) and Candida spp. (30.3%). In spite of their presence in oral cavity in both groups, Candida spp. was more frequently isolated in hospitalized individuals, who were 6.73 times more likely to have this fungus in the oral cavity and were 3.88 times more likely to have Candida albicans.

scholarly journals Surgical Removal of Traumatic Herniation of Buccal Fat Pad in Young Children

2015 ◽  
Vol 11 (3) ◽  
pp. 247-249 ◽  
Author(s):  
NK Agrawal ◽  
S Dahal ◽  
R Khadka
Keyword(s):  
Young Children ◽  
Oral Cavity ◽  
Buccal Mucosa ◽  
Soft Tissues ◽  
Surgical Removal ◽  
Minor Trauma ◽  
Fat Pad ◽  
Buccal Fat Pad ◽  
Traumatic Lesion ◽  
Buccinator Muscle

Intraoral herniation of the buccal fat pad is traumatic lesion of oral cavity occurring in young children. It frequently presents as an expanding pedunculated mass of soft tissue emanating from the buccal mucosa following a minor trauma to the buccal soft tissues. This article aims to report an interesting and rare lesion, wherein a tiny traumatic perforation of the buccal mucosa and buccinator muscle forced a large portion of the buccal fat pad to extrude into the oral cavity in a young boy. Such a situation can alarm any clinician and reinforces the importance of careful history taking and thorough examination, before treating patients. DOI: http://dx.doi.org/10.3126/kumj.v11i3.12514 Kathmandu Univ Med J 2013; 43(3):247-249

scholarly journals Combating dental decay

2005 ◽  
Vol 26 (3) ◽  
pp. 107 ◽  
Author(s):  
Stuart Dashper ◽  
Eric Reynolds
Keyword(s):  
Dental Caries ◽  
Dental Plaque ◽  
Health Surveys ◽  
Tooth Surface ◽  
Tooth Decay ◽  
Dental Services ◽  
Total Cost ◽  
Microbial Biofilms ◽  
Tooth Surfaces ◽  
Eventual Loss

Dental caries or tooth decay is one of the most prevalent bacterial infectious diseases of mankind. In recent oral health surveys, more than 60% of Australian teenagers surveyed had experienced the disease and most dentate adults surveyed exhibited multiple teeth affected by caries. Treating the consequences of dental caries accounts for over 50% of the total cost of providing dental services in Australia, which in 1998 was estimated at $2.6 billion. Dental caries is a dynamic process that is initiated by microbial biofilms on the tooth surfaces (dental plaque) resulting in a disturbance of the equilibrium between tooth mineral and the surrounding plaque fluid so that over time there is a net loss of mineral from the tooth surface. This demineralisation of the enamel may ultimately lead to cavitation of the surface of the tooth and once this stage of the disease has been reached only restorative methods (fillings) can be employed to limit the spread of decay and eventual loss of the tooth.

scholarly journals Impacts of sleep on the characteristics of dental biofilm

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maki Sotozono ◽  
Nanako Kuriki ◽  
Yoko Asahi ◽  
Yuichiro Noiri ◽  
Mikako Hayashi ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Dental Caries ◽  
Oral Cavity ◽  
Periodontal Disease ◽  
Oral Care ◽  
Tooth Surface ◽  
Oral Diseases ◽  
Dental Biofilm

AbstractDental biofilm present on the tooth surface is associated with oral diseases, such as dental caries and periodontal disease. Because bacterial numbers rapidly increase in saliva during sleep, oral care before sleeping is recommended for the prevention of chronic oral diseases. However, temporal circadian changes in the quantity and quality of dental biofilms are poorly understood. This study aimed to investigate the impacts of sleeping on dental biofilm amounts and compositions by using an in situ model. The use of this in situ model enabled us to investigate dental biofilm formed in the oral cavity and to perform a quantitative analysis. Subjects began wearing oral splints in the morning or before sleeping, and biofilm samples were collected at 8, 16, and 24 h after the subjects began wearing oral splints; these samples were then used in various experiments. No significant changes in the numbers of biofilm-forming bacteria were caused by sleep. However, the relative abundances of genera related to periodontitis (i.e., Fusobacterium and Prevotella) increased after awakening. In conclusion, the numbers of biofilm-forming bacteria were not affected by sleep, and the abundances of obligate anaerobes increased after sleep. This research may aid in defining efficacious preventive oral care.

scholarly journals Lobular Capillary Haemangioma of the Oral Cavity in a 11-Year-Old Child - A Case Report

2021 ◽  
Vol 10 (28) ◽  
pp. 2132-2134
Author(s):  
Meghana Vasant Phadnis
Keyword(s):  
Young Adults ◽  
Oral Cavity ◽  
Buccal Mucosa ◽  
Soft Tissues ◽  
Vascular Malformations ◽  
Wide Spectrum ◽  
Case Reports ◽  
Pyogenic Granuloma ◽  
Vascular Lesions ◽  
Capillary Haemangioma

Lobular capillary haemangiomas are benign vascular neoplasms that typically affect the skin or mucous membrane. Majority of the cases reporting lobular capillary haemangioma commonly involve the gingiva. There are rare cases presenting involvement of other parts of the oral cavity like buccal mucosa. The management of vascular lesions is more complex in children due to fear of uncontrollable bleeding. The case presented here reports a successful management of lobular capillary haemangioma of the buccal mucosa in an 11 - year - old child. Vascular lesions are one of the rare disorders affecting the overall quality of life of a child. American Academy of Paediatric Dentistry (AAPD) has presented numerous lesions, masses, or tumour-like conditions of hard and soft tissues within the oral and maxillofacial regions of children and young adults; most of these lesions being mucosal conditions. Oral vascular tumours encompass a wide spectrum of anomalies in children. In spite of the fact that a few cases are congenital, most of them arise during childhood.1 The vascular lesions of childhood are classified into two categories: haemangiomas (proliferating or involuting) and vascular malformations. Haemangioma is a term that envelops a heterogeneous gathering of clinical benign vascular lesions that share similar histologic features and presents as a proliferating mass of blood vessels. Haemangiomas are classified on the basis of their histological1 appearance as capillary, mixed or cavernous haemangiomas depending on the size of the vascular spaces or a sclerosing variety that tends to undergo fibrosis. Capillary haemangiomas are of two types; lobular capillary haemangioma and non-lobular capillary haemangioma depending on their histological features. Lobular capillary haemangioma (LCH) is a common reactive angiomatous proliferation of skin and mucous membranes.2,3 LCH (Mills et al. 1980 ) represents an essential, underlying lesion of pyogenic granuloma (PG).4,5 Based on the available literature and case reports, several clinical and histological differences were seen between PG and LCH, yet there is no clear distinction between the two and still remains a topic of debate. The peak prevalence of LCH is in paediatric population and young adults, with a female predilection. Although LCH is one of the most common soft tissue tumours of head and neck accounting for almost 6 % of all cases, there is a striking predilection for lesions on the gingiva. The occurrence of intraoral haemangiomas at sites such as lips, tongue, buccal mucosa, and palate are extremely rare. LCH is a tumour-like growth of the oral cavity or skin that is considered to be non-neoplastic in nature. The purpose of this paper is to report a case of lobular capillary haemangioma in a paediatric patient and describe the successful treatment of this case.

RELATIONSHIP BETWEEN EDUCATION LEVEL AND KNOWLEDGE OF DENTAL CARIES AND TOOTH BRUSHING SKILLS IN PREGNANT WOMEN IN SOUTH DENPASAR DISTRICT 2021

2021 ◽  
Vol 8 (2) ◽  
pp. 41-50
Author(s):  
I Gede Surya Kencana
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Oral Cavity ◽  
Periodontal Disease ◽  
Pregnant Women ◽  
Tooth Loss ◽  
Soft Tissues ◽  
Education Level ◽  
Tooth Brushing ◽  
Knowledge Level

Dental and oral health is a healthy condition of the hard and soft tissues of the teeth and related elements in the oral cavity that allow individuals to eat, talk and interact socially without dysfunction, esthetic disturbances, and discomfort due to disease, occlusion irregularities and tooth loss so able to live productively socially and economically. The main problems of dental and oral health that are most commonly found in the community are dental caries and periodontal disease. One of the causes of dental caries is the behavior when brushing teeth is wrong because it is done in the morning shower and evening shower and not after breakfast and before bed at night. The purpose of this study was to describe knowledge about dental caries and tooth brushing skills in pregnant women in South Denpasar sub- district in 2021. This study used 40 pregnant women as respondents. The results of this study indicated that the knowledge of pregnant women about dental caries was mostly in the sufficient category, namely 19 people (47.50%). The average knowledge about dental caries in pregnant women was 73.75 with sufficient category. The majority of pregnant women brushing skills in the category of needing guidance were 17 people (42.50%). The average tooth brushing skill among pregnant women was 59.25 with the category of needing guidance. The results of this research on brushing skills based on knowledge of dental caries were that seven pregnant women (36.84%) had the skills to brush their teeth in the category of need guidance with sufficient knowledge of caries category. The Conclusion There is a relationship between education level and knowledge about dental caries in pregnant women and there is a relationship between knowledge level and tooth brushing skills in pregnant women in South Denpasar District.

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