scholarly journals Anti-Proliferative and Pro-Apoptotic Activities of Synthesized 3,4,5 Tri-Methoxy Ciprofloxacin Chalcone Hybrid, through p53 Up-Regulation in HepG2 and MCF7 Cell Lines

2021 ◽  
Vol 22 (10) ◽  
pp. 3393-3404
Author(s):  
Marwa Eisa ◽  
Moustafa Fathy ◽  
Gamal Abuo-Rahma ◽  
Mohamed Abdel-Aziz ◽  
Maiiada Nazmy
Keyword(s):  
Cell Lines ◽  
Mcf7 Cell

scholarly journals Phytochemical Analysis of Anvillea garcinii Leaves: Identification of Garcinamines F–H and Their Antiproliferative Activities

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1130
Author(s):  
Hanan Y. Aati ◽  
Shagufta Perveen ◽  
Raha Orfali ◽  
Areej M. Al-Taweel ◽  
Jiangnan Peng ◽  
...  
Keyword(s):  
Cell Lines ◽  
Liver Diseases ◽  
Mcf7 Cell ◽  
Sesquiterpene Lactones ◽  
2D Nmr ◽  
Phytochemical Analysis ◽  
Antidiabetic Agent ◽  
Arab Region ◽  
New Compounds ◽  
Acid Unit

Anvillea garcinii is a medicinal plant used in the Arab region for intestinal diseases, lung and liver diseases, digestive problems, and as an antidiabetic agent. Repeated chromatographic purifications of A. garcinii leaves led to the isolation of three undescribed guaiane sesquiterpene derivatives, named garcinamines F–H, characterized by the presence of an amino acid unit, along with five known sesquiterpene lactones (garcinamines B–E and 9β-hydroxyparthenolide). The structures of the new compounds were established using spectroscopic (1D and 2D NMR) and spectrometric methods (ESIMS). Garcinamine H possesses a double bond at the Δ1,10 position, a structural feature rarely reported in guaianolide-type sesquiterpenes. The antiproliferative activity of the isolated sesquiterpenes was screened against three different cancer cell lines, and 9β-hydroxyparthenolide and garcinamines C and D displayed significant effects against lung carcinoma (A549), colon carcinoma (LoVo), and breast carcinoma (MCF7) cell lines.

Eryngium billardieri Extract and Fractions Induces Apoptosis in Cancerous Cells

2021 ◽  
Vol 21 ◽  
Author(s):  
Samira Hasanbeiglu ◽  
Kamran Hosseini ◽  
Ommoleila Molavi ◽  
Parina Asgharian ◽  
Vahideh Tarhriz
Keyword(s):  
Cell Lines ◽  
Mcf7 Cell ◽  
Inhibitory Effect ◽  
P Value ◽  
Breast Cancers ◽  
Natural Habitats ◽  
Cytotoxic Effects ◽  
Soxhlet Apparatus ◽  
Mcf7 Cell Line ◽  
Cancerous Cells

Background: Eryngium is genus flowering plants in the Umbelliferae family having pharmacological properties such as anti-inflammatory and anti-diabetic. Given the nature of melanoma and breast cancers in recent years and the fact that the anti-cancer properties of Eryngium billardieri on mentioned cell lines have not been studied, the present study conducted to explore these properties. Objective: The mechanisms of cytotoxicity and apoptosis of aerial parts of various extracts and fractions of E. billardieri on cancerous cells and normal cells were investigated. Methods: Samples were collected from natural habitats, dried and then extracted by Soxhlet apparatus with solvents of n-Hex, DCM and methanol, respectively. The cytotoxic effects of the extracts were investigated by MTT method on MCF7, B16 and HFF-2 classes for 24 and 48 hours. Flowcytometry, was also used to investigate the mechanism of cytotoxicity and confirming by Real-time PCR of p53 and Bax genes, which codes apoptosis regulator proteins. Meanwhile, volatile compounds of extracts were identified by GC-MS method. Results: The obtained data showed that the n-Hex extract of E. billardieri on B-16 and MCF7 cell lines and dichromethane extract on MCF7 cell line had the most significant cytotoxic effect compared to DMSO control (p value <0.001). Our finding showed that the mechanism of n-Hex extract with 80% and 100% vlc fractions on B16 induced apoptotic compared to HFF-2 control cells, moreover, n-Hex extract and 80% vlc fraction on MCF7 was apoptotic. The major compounds of n-Hex, DCM and 80% and 100% fractions of n-Hex extract obtained from GC-MS are non-terpenoid. Conclusion: Non-terpenoids compounds of E. billardieri can be the responsible for showing the most cytotoxic effects on MCF7 and B16 cell lines with apoptotic mechanism and n-Hex extract had the most significant inhibitory effect on cancerous cells compared to the HFF-2 control cells by the mechanism of apoptosis.

scholarly journals A New Triterpene Glucoside from Genista numidica

2018 ◽  
Vol 13 (9) ◽  
pp. 1934578X1801300
Author(s):  
Feryal Benayache ◽  
Massimiliano D'Ambola ◽  
Roberta Cotugno ◽  
Massika Chaouche ◽  
Samir Benayache ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Oleanolic Acid ◽  
Hela Cells ◽  
Antiproliferative Activity ◽  
Mcf7 Cell ◽  
2D Nmr ◽  
Aerial Parts ◽  
Ethyl Acetate Extracts

A new oleanolic acid triterpene glucoside, 3- O-β-D-glucopyranosyl-3β,21β,28-trihydroxy-olean-12-en-27-oic acid (1), has been isolated together with twelve known compounds from the chloroform and ethyl acetate extracts of Genista numidica Spach (Fabaceae) aerial parts. The structures were elucidated by spectroscopic and spectrometric analyses, mainly 1D-, 2D-NMR and MS data, and comparison with the literature. The antiproliferative activity of isolates was investigated on Jurkat, HeLa, and MCF7 cell lines. The most active triterpene, 3- O-β-D-glucopyranosyl-olean-12-en-3β,27,28,29-tetraol, showed activity in all cell lines. Further studies revealed that this compound induced in HeLa cells a cytostatic response.

Synthesis and characterization of ZnO nanoflakes anchored carbon nanoplates for antioxidant and anticancer activity in MCF7 cell lines

2019 ◽  
Vol 102 ◽  
pp. 536-540 ◽  
Author(s):  
Mariadhas Valan Arasu ◽  
A. Madankumar ◽  
J. Theerthagiri ◽  
Sunitha Salla ◽  
S. Prabu ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Cell Lines ◽  
Mcf7 Cell ◽  
Synthesis And Characterization ◽  
Zno Nanoflakes

scholarly journals Styryllactones and Acetogenins from the Fruits of Goniothalamus macrocalyx

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Quy Hung Trieu ◽  
Huong Doan Thi Mai ◽  
Van Cuong Pham ◽  
Marc Litaudon ◽  
Françoise Gueritte ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Cell Lines ◽  
Mass Spectrometer ◽  
Natural Resource ◽  
Absolute Configuration ◽  
Mcf7 Cell ◽  
Crystallographic Analysis ◽  
Kb Cells ◽  
X Ray ◽  
Orbitrap Mass Spectrometer

Two new styryllactones, macrocalactone (1) and 3-deoxycardiobutanolide (2), were isolated from the fruits of Goniothalamus macrocalyx Ban (Annonaceae), together with seven known compounds including four acetogenins, annonacin (3), solamin (4), isoannonacin (5), trans-murisolinone (6), and three other compounds, 7-acetylaltholactone (7), β-caryophyllene-8R,9R-oxide (8) and 2-(2′-hydroxytetracosanoylamino)-octadecane-l,3,4-triol (9). Their structures were determined by spectroscopic and MS analysis. The absolute configuration of I was determined by X-ray crystallographic analysis. The structures of the acetogenins were confirmed by liquid chromatography coupled to a hybrid quadrupole-time of flight mass spectrometer, using post-column lithium infusion. The results were compared with the fragmentation obtained with a hybrid linear trap-orbitrap mass spectrometer. Compound 7 had cytotoxicity against KB, HepG2, Lu, and MCF7 cell lines with IC50 values of 13.1, 23.7, 26.3 and 60.2 μM, respectively, whereas annonacin (3) was selectively active against KB cells (IC50 value of 6.5 μM). The discovery of 3-deoxycardiobutanolide (2) from the fruits of this plant revealed that G. macrocalyx could be a valuable natural resource to obtain this compound as it has been previously reported to have a significant cytotoxicity against different cancer cell lines, especially HL-60 cells.

scholarly journals Synthesis and Cytotoxicity Evaluation of Novel Asymmetrical Mono-Carbonyl Analogs of Curcumin (AMACs) against Vero, HeLa, and MCF7 Cell Lines

2018 ◽  
Vol 86 (2) ◽  
pp. 25 ◽  
Author(s):  
Pekik Wiji Prasetyaningrum ◽  
Anton Bahtiar ◽  
Hayun Hayun
Keyword(s):  
Cell Lines ◽  
Mcf7 Cell

scholarly journals Cytotoxic effect of Spirulina platensis extract and Ulva compressa Linn. on cancer cell lines

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1018-1023
Author(s):  
M. Maryati ◽  
A. Saifudin ◽  
S. Wahyuni ◽  
J. Rahmawati ◽  
A. Arrum ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Spirulina Platensis ◽  
Cytotoxic Effect ◽  
Mcf7 Cell ◽  
Ethanol Extract ◽  
Cancer Cell Lines ◽  
Ulva Compressa

Studies have shown that algae and seaweed have cytotoxic activity. This study was aimed to determine the cytotoxic activity of Spirulina platensis and Ulva compressa Linn. extracts against cancer cell lines. The cytotoxic activity of the extract was carried out using the MTT ((3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. Results showed that the ethanol extract and methanol extract of Spirulina platensis have no cytotoxic effect against HeLa, WiDr, MCF7 and T47D cells. Water extract of Spirulina platensis had no cytotoxic activity on T47D and Vero cells. Water extracts of Spirulina platensis increase MCF7 cell growth. Phycocyanin powder also stimulates MCF7 cell growth. Ethanol extract of Ulva compressa Linn. exhibited potentially cytotoxic activity against MCF7 and moderate cytotoxic against WiDR cells with IC50 values are 31.86 μg/ mL and 104.93 μg/mL, respectively. It can be concluded that extract of Spirulina platensis has no potential to be developed for cancer therapy. Ulva compressa Linn has the potential to be developed as an anti-cancer. Further research for study the mechanism of anticancer of Ulva compressa Linn on MCF7 was needed.

scholarly journals Antioxidant Properties and Cytotoxic Activity of Ethyl Acetate Fraction of Plectranthus amboinicus (Lour.) Spreng. Leaves on HeLa and T47D Cell Lines

2019 ◽  
Vol 10 (1) ◽  
pp. 37
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Panal Sitorus ◽  
Denny Satria ◽  
Rizka Damela Sibuea
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Mcf7 Cell ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
T47d Cell ◽  
Flavonoid Content ◽  
Dpph Method ◽  
Plectranthus Amboinicus

Research into plants with anticancer effects is actively encouraged in orderto discover new drugs with lessertoxicity but more potent effects. The aims of study are to evaluate the antioxidant properties and to investigate the cytotoxic activity of Plectranthus amboinicus (Lour.) Spreng. leaves ethyl acetate fractions on HeLa,T47D and MCF7 cell lines. The extract was prepared by graded maceration using n-hexane and ethyl acetate. The ethyl acetate extract was fractionated in vacuum liquid chromatography with n-hexane: ethyl acetate; and ethyl acetate: methanol as mobile phase. Then, the fractions were analyzed with thin layer chromatography (TLC). The free radical scavenging activity was measured by DPPH method, the total flavonoid content was calculated by quercetin equivalent and the absorbance is measured by using UV-Visible spectrophotometry. The cytotoxic activity were determined using MTT assay. The fractions contained 5 sub fractions with same TLC profile. The fractions showed antioxidant activity by DPPH method with different IC50 values, namely: 130 µg/mL(I), 127 µg/mL(II), 137 µg/mL(III), 129 µg/mL(IV), and 124 µg/ mL(V), respectively. The measurement of total flavonoid content showed 118 mg QE/g (I), 50 mg QE/g (II), 207 mg QE/g (III), 56 mg QE/g (IV), and 55 mg QE/g (V). The IC50 of each sub fractions on HeLa cell were 77 µg/mL, 46 µg/mL, 93 µg/mL, 71 µg/mL and 476 µg/mL; for T47D cell were 1621 µg/mL, 111 µg/mL, 128 µg/mL, 150 µg/mL and 209 µg/mL; and for MCF7 were 259 µg/mL, 343 µg/mL, 575 µg/mL, 408 µg/mL and 250 µg/mL. Based on the results, the fractions derived from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. leaves exhibit antioxidant. The Fraction II from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. was the most cytotoxic on HeLa, T47D and MCF7 cell lines. It is potential to undergo further isolation of its cytotoxic compounds.Keywords : antioxidant, cytotoxic, Plectranthus amboinicul (Lour.) Spreng., ethyl acetate fractions

scholarly journals New Bioactive Sesquiterpeniods From the Plant Endophytic Fungus Pestalotiopsis theae

2021 ◽  
Vol 12 ◽  
Author(s):  
Gaoran Liu ◽  
Ruiyun Huo ◽  
Yanan Zhai ◽  
Ling Liu
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Cell Lines ◽  
Crude Extract ◽  
Endophytic Fungus ◽  
Spectroscopic Data ◽  
Mcf7 Cell ◽  
Cytotoxic Effects ◽  
Electronic Circular Dichroism

Three new secondary metabolites pestalothenins A–C (1–3), including two new humulane-derived sesquiterpeniods (1 and 2) and one new caryophyllene-derived sesquiterpeniod (3), together with five known compounds (4–8) were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae (N635). Their structures were elucidated by the extensive analyses of HRESIMS and NMR spectroscopic data. The absolute configurations of 1–3 were determined by comparison of experimental and calculated electronic circular dichroism (ECD) spectra. The cytotoxic effects of these compounds were evaluated in vitro. Compound 6 showed moderate cytotoxicity against T24 and MCF7 cell lines. In addition, compounds 1–8 were also evaluated for antibacterial activity.

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