scholarly journals Comparative analysis of the antimicrobial potential of different intra-canal medication to microorganisms present in the failure of the endodontic therapy: In vitro study

2020 ◽  
Vol 4 (1) ◽  
pp. 01-06
Author(s):  
Marília Alves
Keyword(s):  
In Vitro Study ◽  
Antimicrobial Treatment ◽  
Etiologic Factor ◽  
New Approach ◽  
Root Canals ◽  
Antimicrobial Potential ◽  
Mechanical Preparation ◽  
Petri Plate ◽  
Micro Organisms

Microorganisms that infect the root canals system are the main etiologic factor of the periapical pathologies. Some microorganisms are resistant to the antimicrobial treatment and may survive in the root canal after the chemical mechanical preparation and intra-canal medication, characterizing a persistent infection. In cases of failure of the endodontic treatment, a new approach may be done using additional measures that involve this infectious process control through the elimination or maximum reduction of microorganisms. Therefore, this article aims to evaluate the antimicrobial potential of different formulations of intra-canal medication compared to strains of Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus in Petri plates. It was used diffusion test in agar where each Petri plate with the inoculated bacteria presented 5 wells that were filled with each medication. The diameters of the bacterial inhibition zones were measured and registered to each tested medication at the period of 24 hours, 48 hours, 1 week and 2 weeks. All the medications promoted inhibition halos; however, a higher elimination of micro-organisms can be significantly achieved through the association of different substances in the formulation of an intra-canal medication, with emphasis to HPG and Ca(OH)2 + CHX.

scholarly journals The Antimicrobial Efficacy of four different Intracanal Medication to Microorganisms existing in the Failure of Endodontic Therapy: An in vitro study

2021 ◽  
Vol 8 (3) ◽  
pp. 01-04
Author(s):  
Jorge Paredes Vieyra ◽  
Fabian Ocampo Acosta ◽  
Francisco Javier Jiménez Enriquez ◽  
Alan Hidalgo Vargas ◽  
Ignacio Manriquez Quintana ◽  
...  
Keyword(s):  
Bacterial Load ◽  
In Vitro Study ◽  
Diffusion Test ◽  
Chemical Combination ◽  
Root Canal System ◽  
Inhibition Zones ◽  
Petri Plate ◽  
Micro Organisms ◽  
And Staphylococcus Aureus

Aim.This study aims to identify the efficacy of different intracanal medication formulations existing in end of endodontic therapy’s failure, and related to strains of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus in Petri plates. Materials and methods. It was used diffusion test in agar where each Petri plate with the inoculated bacteria. Perforations of approximately 4 mm deep by 5 mm in diameter were made to prepare where the intracanal drug (25 µl) to be tested. The diameters of the bacterial inhibition zones were measured and registered to each tested medication at the period of 24 hours, 48 hours, 7 and 14 days respectively. Results. All the medications promoted inhibition halos. The inhibition halos were represented in mm. A higher elimination of micro-organisms can be significantly achieved through the association of different substances in the formulation of an intra canal medication, with emphasis to Ca (OH)2 combined with nitrofurazone and magnesium oxide respectively. Conclusion. Cleaning and shaping of the root canal system associated with the chemical combination of Ca (OH) 2 with antiseptic pastes or solutions considerably reduce the bacterial load.

Comparative characteristic of the degree of apical transport and the centering properties of nickel-titanium tools in the austenitic and martensitic phases

2020 ◽  
pp. 31-41
Author(s):  
Bogdan R. SHUMILOVICH ◽  
Stanislav V. MIKLYAEV
Keyword(s):  
In Vitro Study ◽  
Nickel Titanium ◽  
Comparative Characteristic ◽  
Micro Ct ◽  
Significance Level ◽  
Root Canals ◽  
Mechanical Preparation ◽  
Channel Transport ◽  
Tomography Data

Abstract. The purpose of this in vitro study was to compare the degree of root channel transport and the level of centering of the three most practical endodontic nickel-titanium rotary systems HyFlex EDM, Protaper NEXT and M-two using conical beam computed tomography data. In total, we selected 45 removed from periodontological indications of the first upper jaw molars with fully formed apical sections for the study. A prerequisite for the study was the presence of МВ1 and МВ2 in the anterior buccal root. We randomized all teeth into 3 groups (n = 15) and prepared samples using the files HyFlex EDM, Protaper NEXT and M-two. We evaluated the centering capacity and volume of channel transport by comparing preoperative and postoperative micro-CT scans. We used ANOVA tests, the classification trees method, and the forecast deviation calculation for statistical analysis. The significance level was at 0.05. The channels prepared by means of HyFlex EDM showed the minimum values of extent of transportation of root canals at all three levels (3, 5 and 7 mm from an apical konstriktion) that it connects with a unique design of cross section of a working part of the tool. Maximum channel transport was with M-two in the coronary (7 mm) and middle (5 mm) third. When analyzing centering abilities, channels prepared using the HyFlex EDM and Protaper NEXT system showed maximum values at all three levels compared to M-two files. The channels prepared by means of HyFlex EDM had the minimum values of transportation of the channel at all three levels of a root canal. Optimal mechanical preparation of root channels for the parameters studied at all levels was achieved using the tools HyFlex EDM and Protaper NEXT.

The Antimicrobial Efficacy of four different Intracanal Medication to Microorganisms existing in the Failure of Endodontic Therapy: An in vitro study

2021 ◽  
Vol 8 (3) ◽  
pp. 01-04
Author(s):  
Jorge Vieyra
Keyword(s):  
Bacterial Load ◽  
In Vitro Study ◽  
Diffusion Test ◽  
Chemical Combination ◽  
Root Canal System ◽  
Inhibition Zones ◽  
Petri Plate ◽  
Micro Organisms ◽  
And Staphylococcus Aureus

Aim.This study aims to identify the efficacy of different intracanal medication formulations existing in end of endodontic therapy’s failure, and related to strains of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus in Petri plates. Materials and methods. It was used diffusion test in agar where each Petri plate with the inoculated bacteria. Perforations of approximately 4 mm deep by 5 mm in diameter were made to prepare where the intracanal drug (25 µl) to be tested. The diameters of the bacterial inhibition zones were measured and registered to each tested medication at the period of 24 hours, 48 hours, 7 and 14 days respectively. Results. All the medications promoted inhibition halos. The inhibition halos were represented in mm. A higher elimination of micro-organisms can be significantly achieved through the association of different substances in the formulation of an intra canal medication, with emphasis to Ca (OH)2 combined with nitrofurazone and magnesium oxide respectively. Conclusion. Cleaning and shaping of the root canal system associated with the chemical combination of Ca (OH) 2 with antiseptic pastes or solutions considerably reduce the bacterial load.

scholarly journals Efficiency of a digital electrofulguration system in contaminated root canals in vitro

2021 ◽  
Vol 32 (3) ◽  
pp. 1-9
Author(s):  
Cimara B. B. Silva ◽  
Juliana D. Bronzato ◽  
Daniel R. Herrera ◽  
Francisco Montagner ◽  
Elifas L. Nunes ◽  
...  
Keyword(s):  
Saline Solution ◽  
In Vitro Study ◽  
Bacterial Reduction ◽  
Root Canals ◽  
Solid Media ◽  
Mechanical Preparation ◽  
Colony Forming Units ◽  
Baseline Levels ◽  
Chlorhexidine Gel

Abstract Decontamination of the root canal (RC) system is essential for successful endodontic therapy. In this in vitro study, it was evaluated the disinfection potential of an electrofulguration device, the Endox® Endodontic System (EES), in RC infected with Enterococcus faecalis. Sixty-five human lower premolars were instrumented with MTwo® system. The specimens were distributed into six experimental groups (n = 10), according to the irrigation protocol: 2% chlorhexidine gel (CHX); CHX + EES; 5.25% sodium hypochlorite (NaOCl); NaOCl + EES; saline solution (SS); and SS + EES. Five specimens were untreated (control). RC samples were collected before (C1) and after EES treatment (C2), after chemo-mechanical preparation (CMP) (C3), and after final EES treatment (C4). All samples were plated for colony forming units (CFU/mL) onto solid media. The data were analyzed by Wilcoxon and Friedman tests for intragroup comparisons and by Kruskal Wallis test followed by Dunn’s test for intergroup comparisons (α = 0.05). Treatment with the EES did not significantly reduce the number of CFU/mL as compared to baseline levels (C1 vs. C2, p> 0.05). After CMP (C3), all groups showed a significantly reduced amount of CFU/mL (p <0.05), with no difference between CHX- and NaOCl-treated samples (p >0.05). Lastly, treatment with the EES following CMP (C4) did not significantly reduce the amount of CFU/mL (C3 vs. C4, p> 0.05). To conclude, the use of the Endox® Endodontic System did not result in considerable bacterial reduction at all operative times, while treatment with NaOCl and CHX was equally efficient for this purpose.

scholarly journals The Antimicrobial Efficacy of four different Intracanal Medication to Microorganisms existing in the Failure of Endodontic Therapy: An in vitro study

2021 ◽  
Vol 8 (3) ◽  
pp. 01-04
Author(s):  
Jorge Paredes Vieyra
Keyword(s):  
Bacterial Load ◽  
In Vitro Study ◽  
Diffusion Test ◽  
Chemical Combination ◽  
Root Canal System ◽  
Inhibition Zones ◽  
Petri Plate ◽  
Micro Organisms ◽  
And Staphylococcus Aureus

Aim.This study aims to identify the efficacy of different intracanal medication formulations existing in end of endodontic therapy’s failure, and related to strains of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus in Petri plates. Materials and methods. It was used diffusion test in agar where each Petri plate with the inoculated bacteria. Perforations of approximately 4 mm deep by 5 mm in diameter were made to prepare where the intracanal drug (25 µl) to be tested. The diameters of the bacterial inhibition zones were measured and registered to each tested medication at the period of 24 hours, 48 hours, 7 and 14 days respectively. Results. All the medications promoted inhibition halos. The inhibition halos were represented in mm. A higher elimination of micro-organisms can be significantly achieved through the association of different substances in the formulation of an intra canal medication, with emphasis to Ca (OH)2 combined with nitrofurazone and magnesium oxide respectively. Conclusion. Cleaning and shaping of the root canal system associated with the chemical combination of Ca (OH) 2 with antiseptic pastes or solutions considerably reduce the bacterial load.

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals The IG- file use to Gauge the Apical Diameter in Endodontics: An In Vitro Study

2018 ◽  
Vol 12 (1) ◽  
pp. 638-646 ◽  
Author(s):  
Massimo Amato ◽  
Alfredo Iandolo ◽  
Giuseppe Pantaleo ◽  
Dina Abtellatif ◽  
Michele Simeone ◽  
...  
Keyword(s):  
Measurement Accuracy ◽  
Reference Value ◽  
In Vitro Study ◽  
Vitro Study ◽  
Root Canals ◽  
New Instrument ◽  
Clinician Experience ◽  
Incorrect Measurement ◽  
The Difference

Aim: The aim of this study was to evaluate the efficacy of the IG-file, a new instrument designed for apical diameter gauging. Materials and Methods: After shaping with F1 Universal Protaper, 60 roots were randomly divided into two groups and assigned to two operators, One Expert in Endodontics (EO) and One Unexpert (UO). In each sample, after canal curvatures have been detected, the apical diameters were measured with the IG-file and the K-NiTi. The results were compared with the reference value obtained by retrograde apical gauging. The data were statistically analyzed. Results: Among 60 samples, 10% of errors were recorded when the IG-files were used; in the K-NiTi group the incorrect measurements were 70%. In both groups (expert and unexpert) the IG-file measurements were more accurate than the K-NiTi (90 vs 33 and 90 vs 26,7). The differences were statistically significant. In curved canals, the difference between measurement rates performed with both instruments was statistically significant (85,7% IG-file vs 28,6% K-NiTi) as well as for the samples without curvatures (92,3% IG file vs 30,8% NiTi file). In root canals without curvatures overestimation errors in K-NiTi file group are more frequent than underestimation errors. This difference was statistically significant. Conclusion: A proper gauging of the apical diameter has a key role in endodontic therapy; an incorrect measurement can lead to clinical failures. This “in vitro” study highlights that IG-file improves measurement accuracy independently from clinician experience. Furthermore, in curved canals, the IG-file is more accurate than K-NiTi.

scholarly journals An In-vitro study to compare the effect of four moisture contaminants on the shear bond strength of composite resin

2021 ◽  
Vol 6 (4) ◽  
pp. 212-216
Author(s):  
Lalit Dattu Darade ◽  
Aditi Subhashchandra Sarda ◽  
Kishore Kumar Sangeri ◽  
Pranjali Naresh Patil ◽  
Ughade Snehal P ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Clinical Analysis ◽  
Conventional Technique ◽  
Careful Examination ◽  
Inert Material ◽  
Chi Square ◽  
Detection Rates ◽  
Root Canals ◽  
Naked Eye

The success of an Endodontic treatment relies on the position and morphology of root canals. It is important for a clinician to be familiar with the canal morphology to negotiate and thereby filling the canals with an inert material. But, the possibility of negotiating the canals with the use of conventional technique alone is difficult. Hence, careful examination with the use of advanced techniques to enhance vision is required to minimize the rate of treatment failures. To compare the efficiency of three methods Cone-Beam computed Tomography (CBCT), clinical analysis, AND Dental Loupes in detecting the MB2 canal in maxillary first molars.One hundred and fifty extracted intact permanent molars were collected. Two examiners i.e., one Endodontist and a Radiologist examined CBCT images of teeth for the number and position of root canals. The first stage included unaided examination using an explorer and K- files. While those teeth that were failed to locate the canals were examined using dental loupes for better magnification. The results were analyzed by McNemar's tests with Bonferroni correction, and Chi-square test using SPSS software v21.Among 150 extracted teeth that were compared,MB2 was detected using CBCT in around 68% of the teeth, while with the use of naked eye and dental loupes, it was found to be around 30% and 66% respectively and the difference in the detection rates was statistically significant.With the view of the parameter under consideration, CBCT has high detection rates followed by dental loupes and naked eye vision. The efficiency of CBCT scans and dental loupes in detecting a second mesio-buccal canal, Thus making it more likely to be treated by the clinician.

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