scholarly journals An In-vitro study to compare the effect of four moisture contaminants on the shear bond strength of composite resin

2021 ◽  
Vol 6 (4) ◽  
pp. 212-216
Author(s):  
Lalit Dattu Darade ◽  
Aditi Subhashchandra Sarda ◽  
Kishore Kumar Sangeri ◽  
Pranjali Naresh Patil ◽  
Ughade Snehal P ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Clinical Analysis ◽  
Conventional Technique ◽  
Careful Examination ◽  
Inert Material ◽  
Chi Square ◽  
Detection Rates ◽  
Root Canals ◽  
Naked Eye

The success of an Endodontic treatment relies on the position and morphology of root canals. It is important for a clinician to be familiar with the canal morphology to negotiate and thereby filling the canals with an inert material. But, the possibility of negotiating the canals with the use of conventional technique alone is difficult. Hence, careful examination with the use of advanced techniques to enhance vision is required to minimize the rate of treatment failures. To compare the efficiency of three methods Cone-Beam computed Tomography (CBCT), clinical analysis, AND Dental Loupes in detecting the MB2 canal in maxillary first molars.One hundred and fifty extracted intact permanent molars were collected. Two examiners i.e., one Endodontist and a Radiologist examined CBCT images of teeth for the number and position of root canals. The first stage included unaided examination using an explorer and K- files. While those teeth that were failed to locate the canals were examined using dental loupes for better magnification. The results were analyzed by McNemar's tests with Bonferroni correction, and Chi-square test using SPSS software v21.Among 150 extracted teeth that were compared,MB2 was detected using CBCT in around 68% of the teeth, while with the use of naked eye and dental loupes, it was found to be around 30% and 66% respectively and the difference in the detection rates was statistically significant.With the view of the parameter under consideration, CBCT has high detection rates followed by dental loupes and naked eye vision. The efficiency of CBCT scans and dental loupes in detecting a second mesio-buccal canal, Thus making it more likely to be treated by the clinician.

scholarly journals Comparative evaluation of the efficiency of cone-beam computed tomography, clinical analysis, and dental loupes in detecting the MB2 canal in maxillary first molars. An in-vitro study

2021 ◽  
Vol 7 (4) ◽  
pp. 271-275
Author(s):  
Pranjali Naresh Patil ◽  
M Robert Justin ◽  
Aditi Subhashchandra Sarda ◽  
Snehal Pundlikrao Ughade ◽  
Lalit Dattu Darade ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Cone Beam Computed Tomography ◽  
Clinical Analysis ◽  
Cone Beam ◽  
Careful Examination ◽  
Inert Material ◽  
Detection Rates ◽  
Root Canals ◽  
Naked Eye

The success of an Endodontic treatment relies on the position and morphology of root canals. It is important for a clinician to be familiar with the canal morphology to negotiate and thereby filling the canals with an inert material. But, the possibility of negotiating the canals with the use of conventional technique alone is difficult. Hence, careful examination with the use of advanced techniques to enhance vision is required to minimize the rate of treatment failures. To compare the efficiency of three methods Cone-Beam computed Tomography (CBCT), clinical analysis, AND Dental Loupes in detecting the MB2 canal in maxillary first molars.One hundred and fifty extracted intact permanent molars were collected. Two examiners i.e., one Endodontist and a Radiologist examined CBCT images of teeth for the number and position of root canals. The first stage included unaided examination using an explorer and K- files. While those teeth that were failed to locate the canals were examined using dental loupes for better magnification. The results were analyzed by McNemar's tests with Bonferroni correction, and Chi-square test using SPSS software v21.Among 150 extracted teeth that were compared,MB2 was detected using CBCT in around 68% of the teeth, while with the use of naked eye and dental loupes, it was found to be around 30% and 66% respectively and the difference in the detection rates was statistically significant.With the view of the parameter under consideration, CBCT has high detection rates followed by dental loupes and naked eye vision. The efficiency of CBCT scans and dental loupes in detecting a second mesio-buccal canal, thus making it more likely to be treated by the clinician.

scholarly journals Effectiveness of hand and Mtwo™ instruments in preparing oval-shaped root canals: in vitro study

2016 ◽  
Vol 21 (2) ◽  
Author(s):  
Fernanda Diederichs Coutinho ◽  
Renata Dornelles Morgental ◽  
Simone Bonato Luisi ◽  
Fabiana Vieira Vier-Pelisser ◽  
Patricia Maria Poli Kopper
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Root Apex ◽  
Conventional Technique ◽  
Basic Sequence ◽  
Preparation Technique ◽  
Root Canals ◽  
Canal Area ◽  
Significant Difference

Objective: This study evaluated through stereomicroscopy the effectiveness of hand and rotary instrumentation techniques, either isolated or combined, in the preparation of oval-shaped root canals. Materials and method: Thirty single-rooted human mandibular incisors were selected. After endodontic access and coronal preflaring, teeth were mounted in a modified Bramante muffle and then sectioned transversely at 3 and 6 mm from the root apex. Images of each section were made under a stereomicroscope at 30× magnification. Teeth were reassembled in the muffle and divided into three groups (n=10) according to the root canal preparation technique: GI - rotary preparation with Mtwo™ basic sequence (10/.04; 15/.05; 20/.06; 25/.06), followed by Mtwo™ complementary instruments (30/.05; 35/.04; 40/.04); GII - rotary preparation with Mtwo™ basic sequence, complemented by hand instruments (#30, #35 and #40); GIII - hand instrumentation using the conventional technique (#10 to #40). All instruments were used in brushing motion. The muffles were separated again so that new images of each section could be obtained. The following parameters were evaluated in pre- and postoperative images: root canal area; perimeter; mesiodistal (MD) and buccolingual (BL) diameters; and mesial (M) and distal (D) wall thickness. Next, the values measured in post- and preoperative images were subtracted forcomparison of experimental groups. Data were analyzed using the Kruskal-Wallis test (α=0.05). Results: No significant difference between groups was observed for all parameters. Conclusion: There was no difference among hand, rotary, or combined instrumentation; all techniques were able to increase root canal area, perimeter, and diameter in MD and BL directions. Moreover, the amount of dentin wearon proximal root canal walls was similar for all tested groups.

scholarly journals Effect of Citric Acid on Color Changes of Calcium Silicate-Based Cements an In Vitro Study

2021 ◽  
Vol 11 (5) ◽  
pp. 2339
Author(s):  
Joanna Metlerska ◽  
Till Dammaschke ◽  
Mariusz Lipski ◽  
Irini Fagogeni ◽  
Anna Machoy-Mokrzyńska ◽  
...  
Keyword(s):  
Citric Acid ◽  
Calcium Silicate ◽  
In Vitro Study ◽  
Vitro Study ◽  
Color Measurement ◽  
Color Changes ◽  
Naked Eye ◽  
Root Canal Irrigants ◽  
The Difference

The aim of the present in vitro study was to investigate the effects of 10% and 40% citric acid (CA) on the color of calcium silicate–based cements (CSCs) in comparison to the effects of common root canal irrigants. Samples of six CSCs (n = 6)—ProRoot MTA (Dentsply, Tulsa, OK, USA), Biodentine (Septodont, Saint-Maur-des-Fossés, France), MTA Plus (Avalon Biomed Inc, by Prevest Denpro Limited, Jammu, India), MTA Repair HP (Angelus, Londrina, PR, Brazil), Ortho MTA (BioMTA, Seoul, Korea), and Retro MTA (BioMTA, Seoul, Korea)—were immersed in 10% and 40% CA as well as 15% EDTA, 2% NaOCl, 2% CHX, and 0.9% NaCl for 15 min, 1 h, and 24 h. ΔE values, representing the difference between the final and baseline values of the color components, were then determined using a VITA Easyshade Compact 5.0 spectrophotometer. Naked-eye evaluation of the changes in color and structures of the materials was performed using our own scale. Upon immersion of the materials in both 10% and 40% CA, there were statistically significant differences between spectrophotometric color measurement results for all CSCs (P < 0.05). However, CA does not cause dark discoloration, observable with the naked eye, of any of the materials, such as NaOCl and CHX. Significant statistical differences were also found between all CSCs in terms of submersion duration (P < 0.05). CA, which could be an alternative to EDTA use, caused greater CSCs discoloration and changed some of their structures. Unless required by the therapeutic procedure, clinicians should pay attention to the fact that the irrigant may affect the CSCs discoloration and minimize the contact time of irrigant with CSCs.

scholarly journals Comparison of bacterial microleakage of three bioactive endodontic sealers in simulated underwater diving and aviation conditions

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mehdi Dastorani ◽  
Behnam Malekpour ◽  
Mohsen AminSobhani ◽  
Mohammadsadegh Alemrajabi ◽  
Arezoo Mahdian ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Chi Square ◽  
Custom Made ◽  
Single Cone ◽  
Registration Number ◽  
Chi Square Test ◽  
Pressure Changes

Abstract Background Bacterial microleakage is an important cause of apical periodontitis and endodontic treatment failure. This study aimed to assess the bacterial microleakage of nano-mineral trioxide aggregate (nano-MTA) as a sealer, Endoseal MTA, and GuttaFlow Bioseal sealers in atmospheric pressure, and simulated underwater diving and aviation conditions. Methods In this in vitro, experimental study, 180 extracted single-rooted teeth were cleaned and shaped, and were then randomly divided into three groups for single-cone obturation using Endoseal MTA, GuttaFlow Bioseal, or nano-MTA as a sealer. Each group was then randomly divided into three subgroups, and subjected to ambient atmospheric pressure, 2 atm pressure (to simulate underwater diving), and 0.5 atm pressure (to simulate aviation) using a custom-made pressure chamber. The teeth then underwent microbial leakage test using Streptococcus mutans (S. mutans), and the percentage of samples showing microleakage was recorded for up to 1 month, and analyzed using the Chi-square test. Results The three sealer groups were significantly different regarding bacterial microleakage (P < 0.05). The nano-MTA group showed significantly higher microleakage after 15 days than the other two groups (P = 0.006). The effect of pressure on bacterial microleakage was not significant in any sealer group (P > 0.05). Conclusion Within the limitations of this in vitro study, it may be concluded that single-cone obturation technique using nano-MTA as a sealer results in lower resistance to bacterial microleakage compared with the use of GuttaFlow Bioseal, and Endoseal MTA. Pressure changes in simulated underwater diving and aviation conditions had no significant effect on bacterial microleakage. Trial Registration Number This is not a human subject research.

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals The IG- file use to Gauge the Apical Diameter in Endodontics: An In Vitro Study

2018 ◽  
Vol 12 (1) ◽  
pp. 638-646 ◽  
Author(s):  
Massimo Amato ◽  
Alfredo Iandolo ◽  
Giuseppe Pantaleo ◽  
Dina Abtellatif ◽  
Michele Simeone ◽  
...  
Keyword(s):  
Measurement Accuracy ◽  
Reference Value ◽  
In Vitro Study ◽  
Vitro Study ◽  
Root Canals ◽  
New Instrument ◽  
Clinician Experience ◽  
Incorrect Measurement ◽  
The Difference

Aim: The aim of this study was to evaluate the efficacy of the IG-file, a new instrument designed for apical diameter gauging. Materials and Methods: After shaping with F1 Universal Protaper, 60 roots were randomly divided into two groups and assigned to two operators, One Expert in Endodontics (EO) and One Unexpert (UO). In each sample, after canal curvatures have been detected, the apical diameters were measured with the IG-file and the K-NiTi. The results were compared with the reference value obtained by retrograde apical gauging. The data were statistically analyzed. Results: Among 60 samples, 10% of errors were recorded when the IG-files were used; in the K-NiTi group the incorrect measurements were 70%. In both groups (expert and unexpert) the IG-file measurements were more accurate than the K-NiTi (90 vs 33 and 90 vs 26,7). The differences were statistically significant. In curved canals, the difference between measurement rates performed with both instruments was statistically significant (85,7% IG-file vs 28,6% K-NiTi) as well as for the samples without curvatures (92,3% IG file vs 30,8% NiTi file). In root canals without curvatures overestimation errors in K-NiTi file group are more frequent than underestimation errors. This difference was statistically significant. Conclusion: A proper gauging of the apical diameter has a key role in endodontic therapy; an incorrect measurement can lead to clinical failures. This “in vitro” study highlights that IG-file improves measurement accuracy independently from clinician experience. Furthermore, in curved canals, the IG-file is more accurate than K-NiTi.

scholarly journals Evaluation of Apical Sealing Ability of Four Different Sealers using Centrifuging Dye Penetration Method: An in vitro Study

2012 ◽  
Vol 13 (6) ◽  
pp. 830-833
Author(s):  
Romel Joseph
Keyword(s):  
In Vitro Study ◽  
Blue Dye ◽  
Control Groups ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Sealing Ability ◽  
Ah Plus ◽  
And Control

ABSTRACT Aim The aim of this in vitro study was to evaluate the apical seal obtained with four root canal sealers AH 26, Sealapex, Endoflas FS and AH Plus, with lateral condensation. Materials and methods Sixty root canals were prepared using the step-back technique. The specimens were divided into four experimental groups of 12 teeth and two control groups of 12 teeth. The experimental groups were obturated by laterally condensed gutta-percha with one of the tested sealers and control groups were obturated without any sealer. Methylene blue dye penetration with centrifuging method was used to evaluate the apical sealing ability. The quantitative apical leakage of each specimen was measured after 2 weeks. Results The results showed no significant differences between all groups except between AH Plus and Endoflas FS (<0.05). AH Plus showed significantly less leakage than Endoflas FS. Conclusion AH Plus showed the least leakage compared to AH 26, Sealapex and Endoflas FS. How to cite this article Joseph R, Singh S. Evaluation of Apical Sealing Ability of Four Different Sealers using Centrifuging Dye Penetration Method: An in vitro Study. J Contemp Dent Pract 2012;13(6):830-833.

scholarly journals Microleakage of Packable Composites Used in Post Spaces Condensed Using Different Methods

2002 ◽  
Vol 3 (2) ◽  
pp. 23-30 ◽  
Author(s):  
Güliz Görgül ◽  
A. Cemal Tinaz ◽  
Tayfun Alaçam ◽  
Bağdagül H. Kivanç ◽  
Özgür Uzun
Keyword(s):  
Composite Resin ◽  
In Vitro Study ◽  
Root Canals ◽  
Dye Penetration ◽  
Anterior Teeth ◽  
Mechanical Hand ◽  
Dentin Bonding Agent ◽  
Etched Surface ◽  
Clinical Problems

Abstract The aim of this in vitro study was to evaluate the apical and coronal microleakage of a packable composite material compacted by either hand or ultrasonic condensers when placed in post spaces. Fortytwo extracted anterior teeth were used. After conventional obturation of the root canals, parallel post spaces 5 mm in depth and 2 mm in diameter were prepared using Gates Glidden and diamond burs. A primer and a dentin bonding agent (Prime & Bond NT) was applied to the etched surface for 30 seconds and light polymerized for 40 seconds. Surefil packable composite resin was inserted in four equal increments and each polymerized for 40 seconds. Half of the specimens were compacted with an ultrasonic tip and the other half with a mechanical hand compactor. After immersion in the dye, the teeth were sectioned longitudinally and dye penetration was evaluated using a stereomicroscope and microleakage recorded in mm. A One-Way Analysis of Variance indicated the two condensing methods were not statistically different (p>0.05). When microleakage was compared within groups, there was also no statistical difference between coronal or apical microleakage (p>0.05). In an in vitro environment, ultrasonic condensers do not offer any advantage over hand condensers. Both the coronal and apical portions of the root had microleakage indicating that regardless of the condensation method and the use of a packable composite, there is still microleakage that could potentially create further clinical problems. Citation Görgül G, Alaçam T, Kivanç BH, et. al. Microleakage of Packable Composites Used in Post Spaces Condensed Using Different Methods. J Contemp Dent Pract 2002 May;(3)2: 023-030.

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