ASSESSMENT OF ANTITOXIC AND ANTI-INFECTIOUS ACTIONS OF THE EXPERIMENTAL PREPARATION

In the course of the research, the antitoxic and anti-infectious effects of the developed drug were determined on white mice and white rats. To assess the antitoxic effect in the experiment on white rats, cadmium damage was modeled, followed by taking into account the antidote activity. An increase in the survival rate of animals receiving the drug was found – 60 % versus 40 % in the control, higher hematological indicators: the content of hemoglobin, erythrocytes and leukocytes in the blood of experimental animals was higher than similar control values, respectively, by 3.8, 15.3 and 16,4 %. The anti-infectious effect of the feed additive was determined during the experiment on infecting white mice with the causative agent of Escherichiosis – E. coli (strain "KB-1"). It was found that the introduction of the study drug into the diet of infected animals smoothed the clinical picture of the infectious process, had a hemoprotective effect: the content of erythrocytes, leukocytes, neutrophils, lymphocytes, neutrophils in the blood of experimental animals exceeded the control values, respectively, by 2.5, 13.6, 16, 7, 14.3, 28.6 %. The results of the investigated subject showed that the investigational drug has a well-pronounced antitoxic and anti-infectious effect.

Download Full-text

ASSESSMENT OF RADIO PROTECTIVE AND ADAPTGENIC ACTIONS OF THE EXPERIMENTAL PREPARATION

Scientific Notes Kazan Bauman State Academy of Veterinary Medicine ◽

10.31588/2413-4201-1883-248-4-242-245 ◽

2021 ◽

Vol 248 (4) ◽

pp. 242-245

Author(s):

A.V. Frolov ◽

◽

N.M. Vasilevskiy ◽

F.K. Kalimullin ◽

Z.L. Tuhfatullov ◽

...

Keyword(s):

Survival Rate ◽

Antioxidant Defense ◽

Reduced Glutathione ◽

Lethal Dose ◽

Antioxidant Defense System ◽

Feed Additive ◽

Experimental Animals ◽

White Rats ◽

Adaptogenic Effect ◽

Experimental Preparation

In the course of the research, the radioprotective and adaptogenic effects of the new feed additive were determined in experiments on white mice and white rats. It was found that the inclusion of the studied feed additive in the diet of mice after irradiation provided an increase in the survival rate of animals irradiated with a lethal dose by 50% compared to the control. Feeding the mice with the drug followed by irradiation with a lethal dose increased the survival rate by 42 %. In a similar experiment on white rats, the survival rate of the experimental animals was higher than the control values by 58 and 50 %, respectively. When assessing the adaptogenic effect of the drug, it was found that 12-hour immobilization of rats led to significant changes in the prooxidant-antioxidant defense system. Preliminary feeding of the studied feed additive had a protective effect, which was manifested in a lower content of malondialdehyde in the blood serum of experimental animals, compared with control animals, by 28.0 %, an increased content of catalase by 11.1 %, superoxide dismutase by 43.9 %, reduced glutathione - by 17.6 %. Thus, the investigated drug showed a well-pronounced radioprotective and adaptogenic effect.

Download Full-text

Prevalence of antibiotic-resistant E. coli in broilers challenged with a multi-resistant E. coli strain and received ampicillin, an organic acid-based feed additive or a synbiotic preparation

Poultry Science ◽

10.3382/ps/pez004 ◽

2019 ◽

Vol 98 (6) ◽

pp. 2598-2607 ◽

Cited By ~ 3

Author(s):

Nataliya Roth ◽

Charles Hofacre ◽

Ulrike Zitz ◽

Greg F. Mathis ◽

Karl Moder ◽

...

Keyword(s):

Organic Acid ◽

Coli Strain ◽

Feed Additive ◽

Antibiotic Resistant ◽

E Coli

Download Full-text

Deadly E. Coli Strain May Evade EPA's Test

Chemical & Engineering News ◽

10.1021/cen020811151303 ◽

2011 ◽

pp. 020811151303

Author(s):

Sara Peach

Keyword(s):

Coli Strain ◽

E Coli

Download Full-text

Transport of escherichia coli through soil to groundwater traced by randomly amplified polymorphic DNA (RAPD)

Water Science & Technology ◽

10.2166/wst.1997.0758 ◽

1997 ◽

Vol 35 (11-12) ◽

pp. 351-357 ◽

Cited By ~ 2

Author(s):

R. Rothmaier ◽

A. Weidenmann ◽

K. Botzenhart

Keyword(s):

Negative Impact ◽

Random Amplified Polymorphic Dna ◽

Coli Strain ◽

Soil Samples ◽

Test Field ◽

Liquid Manure ◽

E Coli ◽

Rapd Pattern ◽

Geological Situation ◽

Different Strains

Isolates (50) of E. coli obtained from liquid manure (20 bovine, 20 porcine) were genotyped using random amplified polymorphic DNA (RAPD). Typing revealed 9 and 14 different strains in bovine and porcine liquid manure respectively with no strains in common. One porcine strain, showing a simple RAPD pattern, was subcultured and spread on a test field (1.5l/m2 at 1010 cfu/l) in a drinking water protection zone with loamy to sandy sediments in the Donauried area, Baden-Wurttemberg. Soil samples and groundwaters were collected at monthly intervals October 1994 – June 1995 during which 114 E. coli isolates were recovered. The first occurrence and maximum concentration of E. coli in soil samples taken from more than 20cm depth was in January 1995, declining rapidly with depth and time. All isolates from soil and only one from groundwater showed the RAPD pattern of the spread E. coli strain. The results could not demonstrate a severe negative impact of the spreading of liquid manure on the bacteriological quality of the groundwater in the given geological situation. The distinct strain patterns found in different kinds of liquid manure suggest that genotyping of E. coli by RAPD may be an adequate tool for tracing sources of faecal contamination.

Download Full-text

Low endotoxin E. coli strain-derived plasmids reduced rAAV vector-mediated immune responses both in vitro and in vivo.

Molecular Therapy — Methods & Clinical Development ◽

10.1016/j.omtm.2021.06.009 ◽

2021 ◽

Author(s):

Qingyun Zheng ◽

Tianyi Wang ◽

Xiangying Zhu ◽

Xiao Tian ◽

Chen Zhong ◽

...

Keyword(s):

Immune Responses ◽

Coli Strain ◽

Raav Vector ◽

E Coli

Download Full-text

A Unique Approach for Quantitative Risk Assessment for Cryptosporidiosis and E. coli Strain O157 from Cattle Feces

Proceedings of the Water Environment Federation ◽

10.2175/193864700784994731 ◽

2000 ◽

Vol 2000 (16) ◽

pp. 615-645

Author(s):

John L. Cicmanec

Keyword(s):

Risk Assessment ◽

Coli Strain ◽

Quantitative Risk Assessment ◽

E Coli ◽

Cattle Feces ◽

Unique Approach

Download Full-text

Regulation of Expression of the TIR-Containing Protein C Gene of the Uropathogenic Escherichia coli Strain CFT073

Pathogens ◽

10.3390/pathogens10050549 ◽

2021 ◽

Vol 10 (5) ◽

pp. 549

Author(s):

Julia Ittensohn ◽

Jacqueline Hemberger ◽

Hannah Griffiths ◽

Maren Keller ◽

Simone Albrecht ◽

...

Keyword(s):

Escherichia Coli ◽

Protein C ◽

Interleukin 1 ◽

Coli Strain ◽

Uropathogenic Escherichia Coli ◽

Reporter Construct ◽

Regulation Of Expression ◽

E Coli ◽

Strain Cft073 ◽

Myeloid Differentiation Factor

The uropathogenic Escherichia coli strain CFT073 causes kidney abscesses in mice Toll/interleukin-1 receptor domain-containing protein C (TcpC) dependently and the corresponding gene is present in around 40% of E. coli isolates of pyelonephritis patients. It impairs the Toll-like receptor (TLR) signaling chain and the NACHT leucin-rich repeat PYD protein 3 inflammasome (NLRP3) by binding to TLR4 and myeloid differentiation factor 88 as well as to NLRP3 and caspase-1, respectively. Overexpression of the tcpC gene stopped replication of CFT073. Overexpression of several tcpC-truncation constructs revealed a transmembrane region, while its TIR domain induced filamentous bacteria. Based on these observations, we hypothesized that tcpC expression is presumably tightly controlled. We tested two putative promoters designated P1 and P2 located at 5′ of the gene c2397 and 5′ of the tcpC gene (c2398), respectively, which may form an operon. High pH and increasing glucose concentrations stimulated a P2 reporter construct that was considerably stronger than a P1 reporter construct, while increasing FeSO4 concentrations suppressed their activity. Human urine activated P2, demonstrating that tcpC might be induced in the urinary tract of infected patients. We conclude that P2, consisting of a 240 bp region 5′ of the tcpC gene, represents the major regulator of tcpC expression.

Download Full-text

Novel Bacterial Production of Two Different Bioactive Forms of Human Stem-Cell Factor

International Journal of Molecular Sciences ◽

10.3390/ijms22126361 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6361

Author(s):

Eunyoung Lee ◽

Michelle Novais de Paula ◽

Sangki Baek ◽

Huynh Kim Khanh Ta ◽

Minh Tan Nguyen ◽

...

Keyword(s):

Stem Cell ◽

Ion Exchange ◽

Stem Cell Factor ◽

Transmembrane Domain ◽

Coli Strain ◽

Exchange Chromatography ◽

Soluble Form ◽

Human Stem Cell ◽

E Coli ◽

Human Stem Cell Factor

Human stem-cell factor (hSCF) stimulates the survival, proliferation, and differentiation of hematopoietic cells by binding to the c-Kit receptor. Various applications of hSCF require the efficient and reliable production of hSCF. hSCF exists in three forms: as two membrane-spanning proteins hSCF248 and hSCF229 and truncated soluble N-terminal protein hSCF164. hSCF164 is known to be insoluble when expressed in Escherichia coli cytoplasm, requiring a complex refolding procedure. The activity of hSCF248 has never been studied. Here, we investigated novel production methods for recombinant hSCF164 and hSCF248 without the refolding process. To increase the solubility of hSCF164, maltose-binding protein (MBP) and protein disulfide isomerase b’a’ domain (PDIb’a’) tags were attached to the N-terminus of hSCF164. These fusion proteins were overexpressed in soluble form in the Origami 2(DE3) E. coli strain. These solubilization effects were enhanced at a low temperature. His-hSCF248, the poly-His tagged form of hSCF248, was expressed in a highly soluble form without a solubilization tag protein, which was unexpected because His-hSCF248 contains a transmembrane domain. hSCF164 was purified using affinity and ion-exchange chromatography, and His-hSCF248 was purified by ion-exchange and gel filtration chromatography. The purified proteins stimulated the proliferation of TF-1 cells. Interestingly, the EC50 value of His-hSCF248 was 1 pg/mL, 100-fold lower than 9 ng/mL hSCF164. Additionally, His-hSCF248 decreased the doubling time, increased the proportion of S and G2/M stages in the cell cycle, and increased the c-Myc expression at a 1000-fold lower concentration than hSCF164. In conclusion, His-hSCF248 was expressed in a soluble form in E. coli and had stronger activity than hSCF164. The molecular chaperone, MBP, enabled the soluble overexpression of hSCF164.

Download Full-text