scholarly journals A comparative study of the inhibitory effects by caffeic acid, catechins and their related compounds on the generation of radicals in the reaction mixture of linoleic acid with iron ions

2017 ◽  
Vol 60 (3) ◽  
pp. 162-168 ◽  
Author(s):  
Yuji Matsui ◽  
Yoshie Tanaka ◽  
Hideo Iwahashi
Keyword(s):  
Linoleic Acid ◽  
Comparative Study ◽  
Caffeic Acid ◽  
Iron Ions ◽  
Inhibitory Effects ◽  
Related Compounds

scholarly journals Some polyphenols inhibit the formation of pentyl radical and octanoic acid radical in the reaction mixture of linoleic acid hydroperoxide with ferrous ions

2000 ◽  
Vol 346 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Hideo IWAHASHI
Keyword(s):  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
High Performance ◽  
Octanoic Acid ◽  
Inhibitory Effects ◽  
Ferrous Ions ◽  
Acid Radical ◽  
Spin Resonance ◽  
Related Compounds

Effects of some polyphenols and their related compounds (chlorogenic acid, caffeic acid, quinic acid, ferulic acid, gallic acid, D-(+)-catechin, D-(-)-catechin, 4-hydroxy-3-methoxybenzoic acid, salicylic acid, L-dopa, dopamine, L-adrenaline, L-noradrenaline, o-dihydroxybenzene, m-dihydroxybenzene, and p-dihydroxybenzene) on the formation of 13-hydroperoxide octadecadienoic (13-HPODE) acid-derived radicals (pentyl radical and octanoic acid radical) were examined. The ESR spin trapping showed that chlorogenic acid, caffeic acid, gallic acid, D-(+)-catechin, D-(-)-catechin, L-dopa, dopamine, L-adrenaline, L-noradrenaline, and o-dihydroxybenzene inhibited the overall formation of 13-HPODE acid-derived radicals in the reaction mixture of 13-HPODE with ferrous ions. The ESR peak heights of α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (4-POBN)/13-HPODE-derived radical adducts decreased to 46±4% (chlorogenic acid), 54±2% (caffeic acid), 49±2% (gallic acid), 55±1% [D-(+)-catechin], 60±3% [D-(-)-catechin], 42±1% (L-dopa), 30±2% (dopamine), 49±2% (L-adrenaline), 24±2% (L-noradrenaline), and 54±5% (o-dihydroxybenzene) of the control, respectively. The high performance liquid chromatography-electron spin resonance (HPLC-ESR) and high performance liquid chromatography-electron spin resonance-mass spectrometries (HPLC-ESR-MS) showed that caffeic acid inhibited the formation of octanoic acid radical and pentyl radical to 42±2% and 52±7% of the control, respectively. On the other hand, the polyphenols and their related compounds had few inhibitory effects on the radical formation in the presence of EDTA. Visible absorbance measurement revealed that all the polyphenols exhibiting the inhibitory effect chelate ferrous ions. Above results indicated that the chelation of ferrous ion is essential to the inhibitory effects of the polyphenols.

scholarly journals The inhibition of deoxyribonucleic acid nucleotidyltransferase by stilboestrol derivatives

1968 ◽  
Vol 108 (5) ◽  
pp. 749-753 ◽  
Author(s):  
A R Fahmy ◽  
K Griffiths
Keyword(s):  
Comparative Study ◽  
Deoxyribonucleic Acid ◽  
Inhibitory Effect ◽  
Effective Inhibitor ◽  
Inhibitory Effects ◽  
Alkyl Substitution ◽  
Competitive Kinetics ◽  
Calf Thymus

The inhibition by diethylstilboestrol of DNA nucleotidyltransferase isolated from calf thymus was studied. The inhibition exercised by diethylstilboestrol appears to obey competitive kinetics with respect to DNA primer. The activities of both replicative and terminal enzymes were affected to the same extent. There was no evidence of binding between DNA and diethylstilboestrol. A comparative study of the inhibitory effects of some stilboestrol derivatives is presented. The alkyl substitution in the αα′-positions seem to alter the inhibitory effect of these compounds: dimethylstilboestrol was more inhibitory than stilbene, and diethylstilboestrol was more inhibitory than dimethylstilboestrol. Hexoestrol, in which the αα′-ethylenic linkage is saturated, was the most effective inhibitor.

scholarly journals Inhibitory Effects of Caffeic Acid Phenethyl Ester Analogues on Experimental Lung Metastasis of Murine Colon 26-L5 Carcinoma Cells

2003 ◽  
Vol 26 (5) ◽  
pp. 638-641 ◽  
Author(s):  
Takema Nagaoka ◽  
Arjun H. Banskota ◽  
Yasuhiro Tezuka ◽  
Yuko Harimaya ◽  
Keiichi Koizumi ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Lung Metastasis ◽  
Caffeic Acid Phenethyl Ester ◽  
Carcinoma Cells ◽  
Inhibitory Effects ◽  
Colon 26 ◽  
Murine Colon

A simulated mucus layer protects Lactobacillus reuteri from the inhibitory effects of linoleic acid

2013 ◽  
Vol 4 (4) ◽  
pp. 299-312 ◽  
Author(s):  
R. De Weirdt ◽  
E. Coenen ◽  
B. Vlaeminck ◽  
V. Fievez ◽  
P. Van den Abbeele ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Lactobacillus Reuteri ◽  
Close Contact ◽  
Mucus Layer ◽  
Inhibitory Effects ◽  
Intestinal Mucus ◽  
Gut Microbe ◽  
Protective Environment ◽  
Bacterial Cell Membrane ◽  
High Concentrations

Lactobacillus reuteri is a commensal, beneficial gut microbe that colonises the intestinal mucus layer, where it makes close contact with the human host and may significantly affect human health. Here, we investigated the capacity of linoleic acid (LA), the most common polyunsaturated fatty acid (PUFA) in a Western-style diet, to affect L. reuteri ATCC PTA 6475 prevalence and survival in a simulated mucus layer. Short-term (1 h) survival and mucin-agar adhesion assays of a log-phase L. reuteri suspension in intestinal water demonstrated that the simulated mucus layer protected L. reuteri against the inhibitory effects of LA by lowering its contact with the bacterial cell membrane. The protective effect of the simulated mucus layer was further evaluated using a more complex and dynamic model of the colon microbiota (SHIME®), in which L. reuteri survival was monitored during 6 days of daily exposure to LA in the absence (L-SHIME) and presence (M-SHIME) of a simulated mucus layer. After 6 days, luminal L- and M-SHIME L. reuteri plate counts had decreased by 3.1±0.5 and 2.6±0.9 log cfu/ml, respectively. Upon supplementation of 1.0 g/l LA, the decline in the luminal L. reuteri population started earlier than was observed for the control. In contrast, mucin-agar levels of L. reuteri (in the M-SHIME) remained unaffected throughout the experiment even in the presence of high concentrations of LA. Overall, the results of this study indicate the importance of the mucus layer as a protective environment for beneficial gut microbes to escape from stress by high loads of the antimicrobial PUFA LA to the colon, i.e. due to a Western-style diet.

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