scholarly journals Histopathologic Evaluation of Radio-Protective Effect of Hesperidin on the Liver of Sprague Dawely Rats

2019 ◽  
Author(s):  
Zh Ghorbani ◽  
R Fardid ◽  
G H Haddadi ◽  
A Derakhshanfar ◽  
A Kouhpayeh ◽  
...  
Keyword(s):  
Gamma Radiation ◽  
Protective Effect ◽  
Gamma Irradiation ◽  
Mononuclear Cells ◽  
Normal Liver ◽  
Whole Body ◽  
Control Group ◽  
Sham Control Group ◽  
Histopathologic Evaluation ◽  
Group 2

Objective: In this article aims to investigate the radio-protective effect of hesperidin on the liver of Sprague Dawely rats.Material and Methods: 40 male Sprague Dawely rats were selected randomly and divided into 8 groups. Group 1 did not receive radiation and hesperidin (sham control). Group 2 received only 100 mg/kg body weight (b.w) of hesperidin for 7 consecutive days (HES group); group 3 exposed to dose of 2Gy whole-body gamma radiation (2Gy group), and group 4 and 5 received 50 and 100 mg/kg b.w of HES for 7 consecutive days before 2 Gy gamma radiation, respectively.Group 6 exposed to dose of 8Gy gamma radiation (8Gy group); group 7 and 8 received 50 and 100 mg/kg b.w of HES for 7 days before 8Gy gamma irradiation, respectively. Histopathological evaluation was perfomred 24 hours after radiation.Results: Administration of hesperidin (50 mg/kg b.w, 7 days) before 2Gy of gamma irradiation led to remove inflammatory mononuclear cells in the portal space, but there was still dilation of central veins while hesperidin at a dose of 100 mg/kg before 2Gy gamma radiation was observed with normal liver structure. Microscopic findings in the groups receiving two doses of hesperidin (50 and 100 mg/kg b.w, orally, 7 days), before 8Gy of gamma radiation, were similar in a way that extreme dilation of central veins to be seen, however, there was no capillarization.Conclusion: HES can be offered as a suitable radio-protector in radiotherapy patients and radiation workers.

scholarly journals Zingerone Alleviate Toxicity Induced by Cisplatin or Gamma Radiation in Rats by Modulating Pro-Inflammatory Mediators

2021 ◽  
Vol 5 (5) ◽  
pp. 01-07
Author(s):  
Gehan R. Abdel-Hamid ◽  
Lobna A. Abdel-Aziz ◽  
Mona G. Anany
Keyword(s):  
Oxidative Stress ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Inflammatory Mediators ◽  
Single Injection ◽  
Biological Activities ◽  
Whole Body ◽  
Control Group ◽  
Time Interval ◽  
Group 4

Background: Zingerone is one of the active components of ginger that possesses multiple biological activities and anti-inflammatory properties against either radiation effect or cisplatin toxicity. Purpose: to examine the protective effect of zingerone against gamma radiation (IR) or cisplatin-induced immunotoxicity. Material and Methods: 48 rats were divided into six groups as follows: (group-1); normal control group received distilled water; (group-2); rats received Zingerone orally at a dose of 25 mg/kg b.wt. Once / day for 14 consecutive days (Zing.). (group-3); Rats were given a single injection of Cisplatin at a dose of 7.5 mg/kg b.wt. intraperitoneally (Cispl.). (group-4); Rats exposed to a single dose of 6 Gy whole-body gamma irradiation using 137Cesium source in a Gamma cell 40 (Rad.). (group-5); rats received same dose of Zingerone then they were exposed to gamma radiation as in group 4 (Zing+Rad.). (group-6); rats received Zingerone followed by single injection of Cisplatin at the dose of 7.5 mg/kg b.wt. Intraperitoneally (Zing+Cisp.). Results: Exhibited a significant increase in expression of NF-κB, IL-10, caspase-3, and gene expression of TNF-α as well as oxidative stress biomarkers (MDA and NO) levels accompanied with a reduced level of SOD in either whole body-irradiated or cisplatin-received group. Conversely, pro-inflammatory cytokines levels were significantly decreased with an improvement of oxidative stress in groups that received zingerone. Conclusion: It could be concluded that zingerone exerts its antioxidative activity and immunomodulatory effects through inhibition of pro-inflammatory mediators induced by whole body-gamma irradiation or cisplatin administration at two time interval early and late stage of radiation exposure (after 2 h and one week).Therefore, further studies are required to elucidate the molecular signaling pathway concerning zingerone.

Protective effects of erdosteine against nephrotoxicity caused by gamma radiation in male albino rats

2015 ◽  
Vol 35 (1) ◽  
pp. 21-28 ◽  
Author(s):  
AA Elkady ◽  
IM Ibrahim
Keyword(s):  
Body Weight ◽  
Gamma Radiation ◽  
Gamma Irradiation ◽  
Gastric Tube ◽  
Whole Body ◽  
Control Group ◽  
Albino Rats ◽  
Once Daily ◽  
Renal Lesions ◽  
Radiation Induced

The aim of this study was focused on investigating the possible protective effect of erdosteine against gamma radiation-induced renal lesions in male albino rats. Twenty-eight albino rats were divided into four equal groups as follows: control group, irradiated group (animals subjected to whole-body gamma irradiation at a dose of 5 Gy), treated group (each rat received 100 mg/kg body weight once daily, orally by gastric tube, erdosteine for 1 week), and treated irradiated group (each rat received 100 mg/kg body weight once daily, orally by gastric tube, erdosteine for 1 week, then exposed to whole-body gamma irradiation at a dose of 5 Gy). The results revealed that the administration of erdosteine to rats before irradiation significantly ameliorated the changes occurred in kidney function (creatinine and urea) compared with irradiated group. Also the changes in serum tumor necrosis factor α, interleukin 1β, and interleukin 6 activities were markedly improved compared with the corresponding values of irradiated group. Kidney catalase and glutathione peroxidase (GPx) activities and reduced glutathione concentration showed approximately normal level when compared with the irradiated group. The histopathological results showed distinctive pattern of renal lesions in irradiated group, while in treated irradiated group the renal tissues showed relatively well-preserved architecture. Erdosteine acts in the kidney as a potent scavenger of free radicals to prevent or ameliorate the toxic effects of gamma irradiation as shown in the biochemical and histopathological changes and might provide substantial protection against radiation-induced inflammatory damage.

scholarly journals Tirilazad mesylate (U-74006F) inhibits effects of endotoxin in dogs

1995 ◽  
Vol 268 (5) ◽  
pp. H1847-H1855 ◽  
Author(s):  
H. Zhang ◽  
H. Spapen ◽  
P. Manikis ◽  
P. Rogiers ◽  
G. Metz ◽  
...  
Keyword(s):  
Endotoxic Shock ◽  
Left Ventricular ◽  
Oxygen Extraction ◽  
Volume Index ◽  
Whole Body ◽  
Control Group ◽  
Stroke Work ◽  
Critical Oxygen ◽  
Group 3 ◽  
Group 2

The present study explored the effects of a potent antioxidant, the 21-aminosteroid U-74006F, on the systemic and regional hemodynamics and the oxygen extraction capabilities during endotoxic shock. Twenty-four anesthetized dogs were randomized into three groups. Group 1 (n = 8) served as control. Group 2 (n = 8) and group 3 (n = 8) received 2 mg/kg iv of Escherichia coli endotoxin, followed 30 min later by saline infusion. Group 3 was given U-74006F as an intravenous bolus of 80 micrograms/kg followed by an infusion of 10 micrograms.kg-1.min-1, and group 2 received an equivalent volume of vehicle. Tamponade was induced 30 min later to study the oxygen extraction capabilities of the animals. Compared with the endotoxin-alone group, the U-74006F-treated dogs maintained higher mean arterial pressure, cardiac index, stroke volume index, and left ventricular stroke work index and lower pulmonary vascular resistance. They also showed a higher fractional blood flow to mesenteric and renal beds. Endotoxin administration increased whole body critical oxygen delivery (DO2crit) from 7.7 +/- 2.4 to 12.0 +/- 1.9 ml.kg-1.min-1 (P < 0.05), but U-74006F decreased DO2crit to 7.8 +/- 2.0 ml.kg-1.min-1 (P < 0.05 vs. endotoxin alone). Endotoxin decreased critical oxygen extraction ratio (O2ERcrit) from 75.0 +/- 12.7 to 44.3 +/- 8.7% (P < 0.05), but U-74006F increased O2ERcrit to 64.1 +/- 11.2% (P < 0.05 vs. endotoxin alone). U-74006F also decreased endotoxin-induced elevation of mesenteric and renal DO2crit and markedly increased regional O2ERcrit. Systemic and regional blood lactate concentrations were lower in the U-74006F-treated animals.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Protective Effect of Morocco Carob Honey Against Lead-Induced Anemia and Hepato-Renal Toxicity

2016 ◽  
Vol 39 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Aicha Fassi Fihri ◽  
Noori S. Al-Waili ◽  
Redouan El-Haskoury ◽  
Meryem Bakour ◽  
Afaf Amarti ◽  
...  
Keyword(s):  
Protective Effect ◽  
Renal Toxicity ◽  
Biological Activities ◽  
Kidney Injury ◽  
Lead Toxicity ◽  
Control Group ◽  
Daily Group ◽  
Group 4 ◽  
Group 3 ◽  
Group 2

Background/Aims: Natural honey has many biological activities including protective effect against toxic materials. The aim of this study was to evaluate the protective effect of carob honey against lead-induced hepato-renal toxicity and lead-induced anemia in rabbits. Methods: Twenty four male rabbits were allocated into four groups six rabbits each; group 1: control group, received distilled water (0.1 ml / kg.b.wt /daily); group 2: received oral lead acetate (2 g/kg.b.wt/daily); group 3: treated with oral honey (1g /kg.b.wt/daily) and oral lead (2 g/kg.b.wt/daily), and group 4: received oral honey (1 g/kg.b.wt/daily). Honey and lead were given daily during 24 days of experimentation. Laboratory tests and histopathological evaluations of kidneys were done. Results: Oral administration of lead induced hepatic and kidney injury and caused anemia during three weeks of the exposure. Treatment with honey prevented hepato-renal lead toxicity and ameliorated lead-induced anemia when honey was given to animals during lead exposure. Conclusion: It might be concluded that honey has a protective effect against lead-induced blood, hepatic and renal toxic effects.

scholarly journals PROTECTIVE EFFECT OF CURCUMA LONGA ADMINISTRATION ON LUNG OF MICE EXPOSED TO CADMIUM

2018 ◽  
Vol 11 (10) ◽  
pp. 536
Author(s):  
Suman Sharma ◽  
Amrita Kumari
Keyword(s):  
Heavy Metal ◽  
Body Weight ◽  
Oral Dose ◽  
Oxidative Damage ◽  
Protective Effect ◽  
Curcuma Longa ◽  
Protective Action ◽  
Control Group ◽  
Toxic Heavy Metal ◽  
Group 2

Objective: Cadmium (Cd) is a toxic heavy metal which is introduced into the environment by various anthropogenic and natural activities. It can cause various health problems even at low concentration by inducing oxidative damage in tissues of organisms. Nowadays, the focus has been raised toward the use of herbal treatment against the heavy metal toxicity. Hence, the present study was aimed to investigate the protective effect of curcumin (Cur) against Cd-induced toxicity in the lung of albino mice.Materials and Methods: Albino mice were divided into 4 groups and 5 mice were kept in each group. The experiment was carried out for 15 and 45 days. Group 1 mice were kept as control. Group 2 mice were given an oral dose of 1mg/kg body weight of Cd on alternate days. Group 3 mice were administered an oral dose of 1mg/kg body weight of Cd on alternate days and 100 mg/kg body weight of Cur daily. Group 4 mice were received an oral dose of 100 mg/kg body weight of Cur daily. Autopsies were done on 15 and 45 days post-treatment.Result: Biochemical observations showed an increased level of lipid peroxidation and decreased the activity of antioxidant enzymes, i.e., superoxide dismutase, catalase, and glutathione peroxidase. However, Cur administration improved the level of malondialdehyde and oxidative stress in lung tissue by its antioxidant activity. Furthermore, cotreatment of Cd and Cur ameliorated the antioxidant level.Conclusion: The results of the present experiment showed the protective action of Cur on the Cd-induced oxidative damage in the lung of mice.

Effect of N-butylscopolamine on intestinal uptake of fluorine-18-fluorodeoxyglucose in PET imaging of the abdomen

2000 ◽  
Vol 39 (08) ◽  
pp. 241-245 ◽  
Author(s):  
W. A. Weber ◽  
N. Avril ◽  
M. Schwaiger ◽  
A. Stahl
Keyword(s):  
Pet Imaging ◽  
Test Group ◽  
Normal Liver ◽  
Whole Body ◽  
Control Group ◽  
Intestinal Uptake ◽  
Visual Score ◽  
Frequent Finding ◽  
Tumor Involvement ◽  
Fluorine 18 Fluorodeoxyglucose

Summary Aim: Circumscribed or diffuse intestinal uptake of F-l 8-fluorodeoxyglucose (FDG) is a frequent finding in PET imaging of the abdomen often interfering with correct scan interpretation. The aim of the present study was to determine whether the antiperistaltic agent N-butylscopolamine reduces intestinal FDG-uptake. Methods: Whole body scans from 40 patients with malignant lymphoma and no evidence for intraabdominal tumor involvement were analyzed (6 bed positions; scan start 60 min post injection of approximately 350 MBq FDG; emission time 9 min per position; no attenuation correction). Twenty patients received 20 mg N-butylscopolamine in combination with an intravenous injection of FDG (test group) and 20 patients received only FDG (control group). For analysis, the intensity of bowel loops and diffuse abdominal background were compared to normal liver on a 4 point scale (0 = lowest intensity, 3 = highest) by two experienced nuclear medicine physicians. Furthermore, focal intestinal uptake was evaluated quantitatively by a R0I technique and bowel to liver ratios (b/l) were calculated. Results: Bowel loops had lower intensity and occupied less abdominal regions in the test group than in the control group (visual score 1 vs. 1.5, p = 0.01; abdominal regions 1 of 5 vs. 2.5 of 5, p = 0.04). The visual score for diffuse abdominal background was 0.5 in the test group and 1 in the control group (p = 0.04). Bowel uptake interfered with scan interpretation in 1 of 20 patients in the test group and 6 of 20 patients in the control group (p = 0.01 ). The b/l ratios were 1.5 ± 0.7 in the test group and 2.3 ± 1.4 in the control group (p = 0.08). Conclusion: Administration of N-butylscopolamine reduces intestinal uptake of FDG and may facilitate accurate interpretation of abdominal FDG-PET studies.

scholarly journals The Preventive Effect of Dexmedetomidine Against Postoperative Intra-abdominal Adhesions in Rats

2015 ◽  
Vol 100 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Serdar Kuru ◽  
Osman Bahadir Bozkirli ◽  
Aziz Mutlu Barlas ◽  
Mehmet Esat Duymus ◽  
Mehmet Senes ◽  
...  
Keyword(s):  
Male Rats ◽  
Control Group ◽  
Preventive Effect ◽  
Abdominal Adhesions ◽  
Histopathologic Evaluation ◽  
Group 3 ◽  
Anti Inflammatory ◽  
Group 2 ◽  
Plasma Malondialdehyde ◽  
Pathologic Parameters

Abstract This study aimed to determine the possible preventive effects of dexmedetomidine on postoperative intra-abdominal adhesions. Dexmedetomidine is a highly selective and potent α2 adrenergic agonist with sedative, analgesic, anxiolytic, sympatholytic, hemodynamic, and diuretic properties. In recent years, investigations have shown that dexmedetomidine possesses secondary antioxidant and also anti-inflammatory effects. Thirty Wistar albino male rats were randomized and divided into 3 groups of 10 animals each: group 1, sham-operated; group 2, cecal abrasion + peritoneal dissection; group 3, cecal abrasion + peritoneal dissection followed by daily intravenous injection of 10 μg/kg dexmedetomidine for 10 days. The animals were killed on postoperative day 21. Blood and cecal samples were taken for biochemical and histopathologic evaluation. In this study, biochemical and pathologic parameters were significantly better in the cecal abrasion + peritoneal dissection + dexmedetomidine group when compared with the cecal abrasion + peritoneal dissection group. Tissue malondialdehyde, myeloperoxidase, total sulfhydryl, and catalase were found to be significantly different between the cecal abrasion/peritoneal dissection + dexmedetomidine and the cecal abrasion/peritoneal dissection groups. Plasma malondialdehyde and total sulfhydryl values were also statistically different between these groups (P &lt; 0.05). Statistical analyses of mean pathologic scores showed that the histopathologic damage in the cecal abrasion/peritoneal dissection + dexmedetomidine group was significantly less than the damage in the control group (P &lt; 0.05 for all pathologic parameters). The results of this study show that dexmedetomidine had a significant preventive effect on postoperative intra-abdominal adhesions. We concluded that these effects might be due to antioxidant and anti-inflammatory activities.

scholarly journals EFFECT OF BASIC FITNESS FUNCTION ACCORDING TO WHOLE-BODY VIBRATION STIMULUS WITH SLOPE DURING DEADLIFT

2020 ◽  
Vol 20 (09) ◽  
pp. 2040010
Author(s):  
SEUNG-ROK KANG ◽  
MYOUNG-HWAN KO ◽  
KAP-SOO HAN ◽  
TAE-KYU KWON
Keyword(s):  
Muscle Function ◽  
Fitness Function ◽  
Whole Body Vibration ◽  
Peak Torque ◽  
Trunk Muscles ◽  
Whole Body ◽  
Control Group ◽  
Body Vibration ◽  
Group 2 ◽  
Group 1

We aimed to investigate the effect of basic fitness function according to whole-body vibration (WBV) stimulus with slope during deadlift in adults. A total of 15 subjects performed deadlift exercise with a sound-wave vibrator. The subjects consisted of three groups: no slope and WBV group (control), WBV only group (group 1), and slope and WBV group (group 2). Slope was set at [Formula: see text], and the frequency and amplitude of WBV were 10[Formula: see text]Hz and 5[Formula: see text]mm, respectively. The participants performed Romanian deadlift 2 days a week for 4 weeks, including 10 trials per set and five sets a day. We measured basic fitness function factor including the isokinetic muscle contraction test using biodex system3. All groups showed an increase in strength of approximately [Formula: see text]%. Group 2 showed the highest increase. Moreover, maximal peak torque of the lumbar joint showed an increase trend similar to that of back muscle strength. An increase of 15.72%, 24.86%, and 51.44% was noted in the control, group 1, and group 2, respectively. The findings indicate that WBV exercise with slope is the most efficient exercise protocol for improving muscle function of the trunk. WBV with slope could help stimulate trunk muscles more and efficiently, could result in a more positive effect on muscle function compared with WBV only, and could be included in an exercise program for efficient patient rehabilitation.

In vivo Time-Dependent Radio-Protective Effect of Lycopene Against Whole-Body Gamma Radiation in Mice

2016 ◽  
Vol 19 (2) ◽  
Author(s):  
Mohammadreza Oladi ◽  
Ameneh Sazgarnia ◽  
Mohammad Reza Ghavam Nasiri ◽  
Mostafa Amrollahi ◽  
Hamideh Parhiz ◽  
...  
Keyword(s):  
Gamma Radiation ◽  
Protective Effect ◽  
Time Dependent ◽  
Whole Body

scholarly journals Evaluation of circulating endothelial cells in the rat after acute and fractionated whole-body gamma irradiation

Nukleonika ◽  
2014 ◽  
Vol 59 (4) ◽  
pp. 145-151 ◽  
Author(s):  
Ghassan Al-Massarani ◽  
Khaled Almohamad
Keyword(s):  
Endothelial Cells ◽  
Gamma Irradiation ◽  
Circulating Endothelial Cells ◽  
Whole Body ◽  
Dependent Manner ◽  
Wide Range ◽  
Group 3 ◽  
Group 2 ◽  
Dose Dependent ◽  
Group 1

Abstract Purpose: Damage to vascular endothelial cells is a well recognised complication of the irradiation. Our objective was to determine the gamma-irradiation effect on the rat circulating endothelial cells (CEC). Material and methods: Eight-week old rats were divided into four groups: group 1 - rats were exposed to acute whole- -body gamma irradiation with a wide range of single doses (0.5, 1, 2, 4 and 8 Gy), group 2 - rats were exposed to fractionated low doses of irradiation (0.1, 0.5 and 1 Gy) every three days for two months, group 3 as group 2, but followed by two months of rest, group 4 were control animals. CEC (CD146 positive cells) in group 1 were counted following CD146-based immuno-magnetic separation after one day and one week, as well as at the end of experiment in the other groups. Results: Quantified CEC showed that there was a dose-dependent reduction in CEC count in group 1 (one week after irradiation) and group 2. A partial re-population of CEC was observed at the end of experiment in both group 1 and group 2 compared to control group. Group 3 showed a significant increase in CEC levels as compared with group 2 without reaching the control level. Conclusion: The number of CEC (CD146 positive cells) in rats exposed to whole-body gamma irradiation was reduced in a dose-dependent manner and it partly recovered during the two-month interval after irradiation. We suggest that CEC count may be an indicator of the radiation-induced vascular damage.

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