scholarly journals Fermentative activity in the leaves of garden roses in conditions of humid subtropics of Russia

Author(s):  
K. V. Klemeshova ◽  
A. A. Budarin ◽  
N. N. Karpun

The increased activity of enzymes that counteract the oxidative degradation of substances is one of the physiological and biochemical adaptations of plants to biotic and abiotic factors. The aim of the research was to study the peculiarities of the antioxidant system of garden roses to elaborate a methodology for a comprehensive evaluation of culture in the wet subtropics of the Russian Black Sea coast. The objects of study were garden rose varieties (Rosa × hybrida hort.), belonging to functional groups – bush and large-flowered, and differing in the degree of resistance to the main fungal diseases of the culture in the region (Diplocarpon rosae FA Wolf, Podosphaera pannosa (Wallr.) De Bary, Botrytis cinerea Pers., Phragmidium mucronatum (Pers.) Schltdl.) The activity of the antioxidant system was evaluated by the activity of the enzymes catalase and peroxidase (guaiacol). Catalase activity (CAT) in physiologically mature rose leaves was determined by the gasometric method according to I. I. Gunar, peroxidase (GPO) by the modified method according to I. P. Ermakov, according to the absorption spectrum (wavelength – 440 nm), recorded on the spectrophotometer PE-5400vi. The studies were carried out in dynamics during the growing season in 2016-2017. The article presents data on changes in the activity of enzymes of the primary antioxidant system – catalase and peroxidase, in the leaves of bush and large-flowered varieties of garden roses, which differ in the degree of resistance to the main phytopathogens of the culture in the region. Variety differences in the activity of enzymes, the dynamics of changes in the activity of antioxidant enzymes during the growing season, the dependence of the enzymatic activity on the hydrothermal conditions of the humid subtropical climate of the Sochi Black Sea Region have been determined. Diagnostic parameters have been established to develop the methodology for a comprehensive evaluation of landscape gardening roses in the region. When developing methods for a comprehensive evaluation of garden roses, the activity of guaiacol peroxidase can be used to analyze the adaptive potential of the culture in the region.

Author(s):  
A. V. Holodna ◽  
N. H. Buslaeva

Purpose. Determination of the influence of seed biodisinfectant, stimulator of growth and foliar feeding on the white lupine growth and development in the context of climate change. Methods. Field (to study the interaction of the object of study with biotic and abiotic factors); morphophysiological (for biological control over development of productivity elements at the stages of organogenesis); weight (to establish the indicators parameters of the crop structure elements and determine the grain yield); statistical (statistical processing of the research results). Results. The results of the analysis of the indicators of the average daily air temperature and the amount of precipitation from April to August and their comparison with long-term values are presented. Indicators of height and aboveground mass of white lupine plants in dynamics, depending on the pre-sowing seed treatment, plant growth stimulant and foliar feeding, correlation dependences of these indicators on the hydrothermal conditions of the growing season have been established. Conclusions. The growth and development of white lupine plants depends on the elements of the technology of its cultivation, as well as hydrothermal conditions during the growing season of the crop. The highest plants (83.0 cm) with their maximum weight (143,8 g/plant) were noted in the variant where the seeds of white lupine were treated with БТУ-р bioinoculant simultaneously with the MicoHelp biodisinfectant and foliar feeding of plants with micronutrient fertilizer Trazex at the ІХ stage of organogenesis was carried out. Mathematical models have been developed that confirm the close relationship between the level of height indicators and the aboveground mass of white lupine plants with weather conditions, the critical periods of their influence in the ontogenesis of plants were also determined.


2016 ◽  
Vol 24 ◽  
pp. 43-51
Author(s):  
O. V. Sherstoboeva ◽  
O. S. Demianiuk

We analyzed the functional and taxonomic structure of microbial community of dark grey soil, depending on the effects of the weather and fertilizer systems. It was shown that fallow soil is characterized by integrated and more stable microbial community compared with agroecosystems soil. However, under the effect of such abiotic factors as increased average monthly temperatures and moisture deficiency, some imbalance of trophic interactions in microbiocenosis is observed. Agroecosystems are characterized by less stable functional and taxonomic structure of the soil, which is defined by the hydrothermal conditions of the growing season and types of fertilizers applied. This is supported by a smaller number of correlations and a simplified structure of correlation pleiades of the soil of agroecosystems during unfavourable periods of vegetation periods, especially in the soil without fertilizers and using only mineral fertilizers. The use of organic and mineral fertilizer system brings the state of the soil microbiocenosis of agroecosystems soil to that of fallow soil.


2017 ◽  
Vol 4 (3) ◽  
pp. 43-49
Author(s):  
M. Miroshnychenko ◽  
O. Siabruk

Aim. The comparison of the effect of hydrothermal conditions and various agricultural practices on the emission of CO 2 from chernozems in the Left-Bank Forest-Steppe of Ukraine. Methods. The dynamics of the intensity of carbon dioxide emissions from chernozem calcic (typical chernozem – in Ukrainian classifi cation) was studied during the growing season of 2011–2012. The observations were based on two fi eld experiments with various methods of soil till- age (6–7 years from the beginning of the experiment) and fertilization systems (21–22 years from the beginning of the experiment). Particularly, plowing at 20–22 cm, disking at 10–12 cm, cultivation at 6–8 cm and direct seeding using Great Plains drill were studied among the soil tillage methods. Mineral system (N 45 P 50 K 45 ), organic system (manure 8 t/ha) and combined organic-mineral system (manure 8 t/ha + N 45 P 50 K 45 ) were studied among fertilization systems. The intensity of CO 2 fl ux was determined using the non-stationary respiratory chambers by the alkaline absorption method, with averaging of the results during the day and the frequency of once a month. Results. During the warm period, the emission of carbon dioxide from the soil changes dynamically depending on temperature and humidity. The maximum of emission coincides with the periods of warm summer showers in June-July, the minimum values are characteristic for the late autumn period. The total emission losses of carbon in chernozems over the vegetation period ranged from 480 to 910 kg/ha and varied depending on the methods of tillage ± (4.0–6.0) % and fertilization systems ± (3.8–7.1) %. The changes in the intensity of CO 2 emission from the soil under different methods of soil tillage are associated with hydrothermal regime and the depth of crop residues location. The biggest difference is observed im- mediately after tillage, but in the spring period the differences are only 12–25 %, and after drying of the top layer of soil become even less. Direct seeding technology provides the greatest emission of CO 2 from chernozem, which is fa- cilitated by better water regime and more complete mineralization of plant residues on the soil surface. Annual losses of carbon are the least under disking of soil at 10–12 cm. The changes in the intensity of CO 2 emission from the soil under different fertilization systems are associated with the involvement of the additional organic matter from plant residues and manure to the microbiological decomposition. The greatest emission was observed under the organic- mineral fertilization system, which increased the loss of carbon by 7–8 % in comparison with the mineral system in the unfavorable hydrothermal year and by 11–15 % in the more favorable year. These differences are observed mainly during the fi rst half of the growing season when there is a clear tendency to increase the intensity of soil respiration. Conclusions. The hydrothermal conditions of the warm period of the year are decisive in the formation of the CO 2 emission fl ow from chernozems. Due to the improvement of agricultural practices, emissions might be reduced but not more that by 15 % of natural factor contribution.


2014 ◽  
Vol 25 (1-2) ◽  
pp. 69-82 ◽  
Author(s):  
L. P. Kharchenko ◽  
I. A. Lykova

Seasonal migration for birds – distant migrants are the most energy intensive. Fat reserves accumulated in the bird’s body before migration and during migratory stopovers determine success of the long-distance flight. Lipids play a vital role both as a source of energy and as structural components of cell membranes. For most migrants to the speed and quality processes fat accumulation affects the feed ration in the field of migration stops. Fodder saturation with essential polyunsaturated fatty acids (PUFAs) is of great importance. Being paramount for physiological processes, these acids cannot be synthesized in the bird’s body. The proposed article is dedicated to the study of waders’ trophic relationships with their prey items, and the use of PUFAs as biochemical markers. This approach is based on the specificity of the fatty acids contained in the lipids of invertebrate to be used as food bird species studied. Significant amount NPZHK waders obtained from forage that can be considered PUFAs as biochemical markers to determine the range and diversity of food producing birds PUFAs ways, and also to study the food chain in ecosystems. A fatty acid spectrum (FAS) of the lipids common for nine littoral invertebrate species (Gammarus aequicauda, Idotea balthica, Artemia salina, Nerеis sp., Nerеis zonata, Theodoxces astrachanicus, Hydrobia acuta, Chironomus salinarius, Chironomus plumosus), which constitute the main component of waders’ diet at the migratory stopover sites in the Azov and Black Sea region, has been studied. Found that the largest amount of total lipids contained in Nereis zonata (4,6 %) and Artemia salina (4,4 %), the lowest amount of total lipids was observed in Chironomus (1,5–1,8 %), which implies that polychaete worms and Artemia salina, as a source of fat, are the most productive for waders. Our research has found that mollusks, polychaete worms, and Artemia salina are the most effective waders’ fodder in the PUFAs content. Mollusks contain the largest amount of PUFAs, their spectrum is ω3 and ω6 PUFAs, especially arаchidonic acid C20:4. Polychate worms are also characterized by a high PUFAs level; they serve for birds as a source of linolenic and linoleic acid groups. Artemia salina contains a large amount of eicosapentaenoic С20:5ω3 and docosahexaenoic С22:6ω3 acids, which getting to an organism of birds, participating in the formation of cell membranes, act as thermal stabilizer lipid bilayers, enhance stamina during long-distance flight. A high abundance of Artemia salina in the feeding areas permit tundra waders to use them as a prey item, which can fulfill the bird’s body with a required amount of fatty acids in a short time. We have established an influence of some environmental factors, as water temperature and salinity, on the lipids FAS of littoral invertebrates. Spectrum analysis of polyene fatty acids in the lipids closely related species of invertebrates living in different salinity water showed that the content of PUFAs in the lipid depend on their food spectrum, and the environmental conditions. Therefore, anthropogenic pressure and changes of water hydrological regime may affect PUFAs content in the lipids of littoral invertebrates. In its turn, this factor may change alimentary behavior and migratory strategy of the birds, which use migratory stopover sites in the region in question. Shallow waters of the Azov-Black Sea region are characterized by different climatic characteristics and a large reserve of phytoplankton. This explains the mass character species studied of invertebrates to feeding areas and their use as basic prey items, many species of waders.


2019 ◽  
Vol 53 (4) ◽  
pp. 297-312
Author(s):  
Yu. O. Andryushchenko ◽  
V. S. Gavrilenko ◽  
V. A. Kostiushyn ◽  
V. N. Kucherenko ◽  
A. S. Mezinov ◽  
...  

Abstract In the article is analyzed own field data of the authors and scientific publications on the wintering of Anserinae in the Azov-Black Sea region of Ukraine in 1900–2017, but the main data was obtained in frame of international mid-winter counts (IWC) in 2005–2017. It was found that 9 species of Anserinae occur in this region during the different seasons of the year: Anser anser — nesting, wintering and migrating; Rufibrenta ruficollis, A. albifrons, A. erythropus, A. fabalis — migrating and wintering; Branta canadensis, Branta leucopsis, Branta bernicla, Chen caerulescens — vagrant or birds which flew away from captivity (zoo etc.). Eulabeia indica — is possible vagrant species. The most numerous wintering species is A. albifrons, common — Rufibrenta ruficollis, not numerous — Anser anser, the other species are not met annually and registered in a very small number. There was almost tenfold drop in number of wintering geese in the Azov-Black Sea region of Ukraine during the period of counts. The main reasons of such reducing of geese amount are the followwing: weather and climate conditions, changes in the forage acessibility, hunting and poaching pressure, poisoning as a result of deratization of agricultural lands, and from 2014 — the militarization of the Syvash area and stop of water supplying of Crimea through the North Crimean channell. It is likely that the factors mentioned above led to relocating of wintering areas of Anserinae, and resulted in decreasing of their amount in this region.


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