scholarly journals Management, operational, animal health, and economic characteristics of large dairy herds in 4 states in the Upper Midwest of the United States

2017 ◽  
Vol 100 (11) ◽  
pp. 9466-9475 ◽  
Author(s):  
T.L. Evink ◽  
M.I. Endres
Keyword(s):  
United States ◽  
Animal Health ◽  
The United States ◽  
Dairy Herds ◽  
Upper Midwest

scholarly journals Co-Occurrence of 35 Mycotoxins: A Seven-Year Survey of Corn Grain and Corn Silage in the United States

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 516
Author(s):  
Alexandra C. Weaver ◽  
Daniel M. Weaver ◽  
Nicholas Adams ◽  
Alexandros Yiannikouris
Keyword(s):  
United States ◽  
Animal Health ◽  
Fusaric Acid ◽  
Fumonisin B1 ◽  
The United States ◽  
Ultra Performance Liquid Chromatography ◽  
Liquid Chromatography Tandem Mass ◽  
And Performance ◽  
The Impact ◽  
Corn Grain

Mycotoxins contaminate crops worldwide and play a role in animal health and performance. Multiple mycotoxins may co-occur which may increase the impact on the animal. To assess the multiple mycotoxin profile of corn (Zea mays), we conducted a 7-year survey of new crop corn grain and silage in the United States. A total of 711 grain and 1117 silage samples were collected between 2013 and 2019 and analyzed for the simultaneous presence of 35 mycotoxins using ultra-performance liquid chromatography–tandem mass spectrometry. The measured mean number of mycotoxins per sample were 4.8 (grain) and 5.2 (silage), ranging from 0 to 13. Fusaric acid (FA) was most frequently detected in 78.1 and 93.8% of grains and silages, respectively, followed by deoxynivalenol (DON) in 75.7 and 88.2% of samples. Fumonisin B1 (FB1), fumonisin B2 and 15-acetyl-deoxynivalenol (15ADON) followed. The greatest (p < 0.05) co-occurrence was between FA and DON in 59.1% of grains and 82.7% of silages, followed by FA with FB1, DON with 15ADON, and FA with 15ADON. Although many samples had lower mycotoxin concentrations, 1.6% (grain) and 7.9% (silage) of tested samples had DON ≥ 5000 µg/kg. Fumonisins were detected ≥ 10,000 µg/kg in 9.6 and 3.9% of grain and silage samples, respectively. Concentrations in grain varied by year for eight mycotoxin groups (p < 0.05), while all 10 groups showed yearly variations in silage. Our survey suggest that multiple mycotoxins frequently co-occur in corn grain and silage in the Unites States, and some of the more prevalent mycotoxins are those that may not be routinely analyzed (i.e., FA and 15ADON). Assessment of multiple mycotoxins should be considered when developing management programs.

scholarly journals Positivity Rates of in Vitro Inhalant/ Respiratory and Food Allergy Tests in the Northern Midwestern United States

2018 ◽  
Vol 97 (9) ◽  
pp. 296-322 ◽  
Author(s):  
Michael S. Benninger ◽  
Thomas Daly ◽  
Kevin Graffmiller
Keyword(s):  
United States ◽  
Cleveland Clinic ◽  
The United States ◽  
Test Results ◽  
Dust Mites ◽  
Upper Midwest ◽  
Allergy Test ◽  
Wide Variability ◽  
Very High

Rates of allergy-test positivity vary by country and by regions within countries. Several studies have looked at allergy test results to determine the most common allergens. Many of these studies have been based on surveys or on studies of small numbers of tests. Positivity rates for allergy tests are poorly defined in the northern midwestern region of the United States. We conducted a study to identify the rates of positive allergy tests for both inhalant/respiratory allergens and food allergens in the upper Midwest. We extracted from our laboratory database the results of all test samples sent for one of eight allergen panels that had been analyzed between Sept. 1, 2014, and Sept. 1, 2015. All testing was performed at The Cleveland Clinic with the Phadia ImmunoCAP system. The percentage of positive tests, the distribution of the most frequently positive tests, and the class of in vitro responses were identified. A total of 148,628 test results for 63 different allergens were identified. Of the 125,190 tests for inhalant/respiratory allergens, the most frequently positive were dog dander (24% of tests), cat dander (23%), dust mites (23% for both Dermatophagoides pteronyssinus and Dermatophagoides farinae), and June grass (21%). Of the 23,438 food tests, the most frequently positive test results were for milk (18%), peanut (17%), wheat (16%), and egg white (15%). Most of the results fell into classes 1 through 3, although there was still a notable number of very high responses (class 5 and 6). These findings suggest that there is wide variability in the positivity of in vitro allergy tests and that the likelihood of a positive result in screening panels can be estimated. Evaluating such rates will help identify the most and least common allergens and will help to cost-effectively refine allergy screening panels.

scholarly journals West Nile Virus Recombinant DNA Vaccine Protects Mouse and Horse from Virus Challenge and Expresses In Vitro a Noninfectious Recombinant Antigen That Can Be Used in Enzyme-Linked Immunosorbent Assays

2001 ◽  
Vol 75 (9) ◽  
pp. 4040-4047 ◽  
Author(s):  
Brent S. Davis ◽  
Gwong-Jen J. Chang ◽  
Bruce Cropp ◽  
John T. Roehrig ◽  
Denise A. Martin ◽  
...  
Keyword(s):  
United States ◽  
Recombinant Plasmid ◽  
Animal Health ◽  
The United States ◽  
Virus Antigen ◽  
Recombinant Antigen ◽  
Immunoglobulin M ◽  
E Proteins ◽  
West Nile ◽  
Immunosorbent Assays

ABSTRACT Introduction of West Nile (WN) virus into the United States in 1999 created major human and animal health concerns. Currently, no human or veterinary vaccine is available to prevent WN viral infection, and mosquito control is the only practical strategy to combat the spread of disease. Starting with a previously designed eukaryotic expression vector, we constructed a recombinant plasmid (pCBWN) that expressed the WN virus prM and E proteins. A single intramuscular injection of pCBWN DNA induced protective immunity, preventing WN virus infection in mice and horses. Recombinant plasmid-transformed COS-1 cells expressed and secreted high levels of WN virus prM and E proteins into the culture medium. The medium was treated with polyethylene glycol to concentrate proteins. The resultant, containing high-titered recombinant WN virus antigen, proved to be an excellent alternative to the more traditional suckling-mouse brain WN virus antigen used in the immunoglobulin M (IgM) antibody-capture and indirect IgG enzyme-linked immunosorbent assays. This recombinant antigen has great potential to become the antigen of choice and will facilitate the standardization of reagents and implementation of WN virus surveillance in the United States and elsewhere.

scholarly journals The Online Sale of Antibiotics for Veterinary Use

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 503 ◽  
Author(s):  
Juan F. Garcia ◽  
M. Jose Diez ◽  
Ana M. Sahagun ◽  
Raquel Diez ◽  
Matilde Sierra ◽  
...  
Keyword(s):  
United States ◽  
United States Of America ◽  
Bacterial Resistance ◽  
Animal Health ◽  
The United States ◽  
Essential Medicines ◽  
The Internet ◽  
Veterinary Antibiotics ◽  
Inappropriate Use ◽  
Simple Search

Antibiotics are essential medicines against infectious diseases in both humans and animals. An inappropriate use of antibiotics can impair animal health and enhance the risk of bacterial resistance, as well as its transfer from animals to humans. The objective of this study was to assess the possibility of purchasing antibiotics for veterinary use on the internet, to evaluate if a prescription is required, and to determine the availability of drugs classified as the highest priority critically important antimicrobials (HP-CIA). The Google and Bing search engines and both simple and complex search strings in Spanish and in English were used. The simple search string was “buy veterinary antibiotics”. Complex searches used wildcards and specific syntax. The searches carried out in Spanish revealed that 50% of websites operated in South America, and 65% of websites did not require a valid prescription. Fluoroquinolones were offered in 84% of these websites (45% without prescription), macrolides were offered in 63% of these websites (43% without prescription), and 3rd– and 4th–generation cephalosporins in 54% of these websites (38% without prescription). For the searches in English, 57% of these websites operated in the United States of America (USA), and 55% of them did not require a prescription. Fluoroquinolones were offered in 79% of these websites (49% without prescription), macrolides were offered in 72% of these websites (45% without prescription), and 3rd– and 4th–generation cephalosporins were offered in 49% of these websites (27% without prescription). Therefore, it is easy to illegally access antibiotics via the internet.

scholarly journals Bovine leukemia virus seroprevalence among cattle presented for slaughter in the United States

2017 ◽  
Vol 29 (5) ◽  
pp. 704-706 ◽  
Author(s):  
Fernando V. Bauermann ◽  
Julia F. Ridpath ◽  
David A. Dargatz
Keyword(s):  
United States ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Economic Loss ◽  
The United States ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dairy Herds ◽  
Positive Rate ◽  
Dairy Animals ◽  
Mountain West

Infection with bovine leukemia virus (BLV) results in economic loss because of reduced productivity, especially reduced milk production, and early culling. In the United States, studies in 1996, 1999, and 2007 showed BLV infection to be widespread, especially in dairy herds. We updated information herein on BLV seroprevalence in the United States, using samples submitted for testing and found negative for antibodies for Brucella by the Kentucky Eastern Regional Federal Brucellosis Laboratory. From October 2014 through August 2015, 2,000 samples from all regions of the contiguous United States were selected and tested for BLV antibodies by enzyme-linked immunosorbent assay. The overall percentage of samples positive for BLV antibody was 38.6%. Based on the animal’s origin, the percent positive by region ranged from 32.5% (Mountain West region) to 54.3% (Northeast region; p < 0.05). The positive rate for slaughter plants that processed mainly dairy animals (dairy plants; 47.6%) was higher than the positive rate at slaughter plants that processed mainly beef animals (beef plants; 33.6%; p < 0.05). The results suggest that BLV infection remains widespread in all regions of the United States and that rates may differ between beef and dairy cattle.

Preparing for Agroterror: How is the Texas Animal Health Commission Implementing Federal Food Security Regulations?

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Jordan Hunter
Keyword(s):  
United States ◽  
Food Supply ◽  
Large Scale ◽  
Animal Health ◽  
Health And Safety ◽  
The United States ◽  
United States Government ◽  
Agricultural Industry ◽  
The Public ◽  
Department Of Health

AbstractAgroterrorism is a subform of bioterrorism with the potential to have a crippling impact on both the agricultural industry and the food supply of a nation. A calculated attack using a miniscule amount of pathogenic or disease causing substances on the livestock or crops in one rural community can spread to animals and metropolitan regions much farther away long before any response from state or federal veterinary or agricultural organizations. Although there have been no large-scale agroterrorism attacks in the United States, there have been historical precedents for agricultural biological warfare and recent examples of unintentional or accidental spread of pathogens in the food supply that have threatened the health and safety of the public. Along with an ongoing push for preparedness to prevent a biological attack on the U.S. agricultural industry, there is a great deal of uncertainty and conflict among landowners, farmers, analysts, and politicians about what methods should be implemented to safeguard the public. In response to this possible threat, the United States government has implemented legislation that it considers preemptive in its ability to safeguard the food supply and manage the public health and/or biological crime response in a widespread agroterrorist attack scenario. However, this requires cooperation on both the state and federal levels, and of several agencies including the department of Health, U.S. Department of Agriculture (USDA), Federal Bureau of Investigation, and the Department of Health and Human Services. This paper examines the steps being taken by the USDA in fulfilling the orders of the federal government in response to the looming threat of agroterrorism and how the TAHC works cooperatively with federal agencies and the private agricultural industry to carry out these regulations.

scholarly journals The enigma of the apparent disappearance of Eurasian highly pathogenic H5 clade 2.3.4.4 influenza A viruses in North American waterfowl

2016 ◽  
Vol 113 (32) ◽  
pp. 9033-9038 ◽  
Author(s):  
Scott Krauss ◽  
David E. Stallknecht ◽  
Richard D. Slemons ◽  
Andrew S. Bowman ◽  
Rebecca L. Poulson ◽  
...  
Keyword(s):  
United States ◽  
North America ◽  
North American ◽  
Influenza A ◽  
Wild Bird ◽  
Animal Health ◽  
The United States ◽  
Influenza Viruses ◽  
Influenza A Viruses ◽  
Highly Pathogenic

One of the major unresolved questions in influenza A virus (IAV) ecology is exemplified by the apparent disappearance of highly pathogenic (HP) H5N1, H5N2, and H5N8 (H5Nx) viruses containing the Eurasian hemagglutinin 2.3.4.4 clade from wild bird populations in North America. The introduction of Eurasian lineage HP H5 clade 2.3.4.4 H5N8 IAV and subsequent reassortment with low-pathogenic H?N2 and H?N1 North American wild bird-origin IAVs in late 2014 resulted in widespread HP H5Nx IAV infections and outbreaks in poultry and wild birds across two-thirds of North America starting in November 2014 and continuing through June 2015. Although the stamping out strategies adopted by the poultry industry and animal health authorities in Canada and the United States—which included culling, quarantining, increased biosecurity, and abstention from vaccine use—were successful in eradicating the HP H5Nx viruses from poultry, these activities do not explain the apparent disappearance of these viruses from migratory waterfowl. Here we examine current and historical aquatic bird IAV surveillance and outbreaks of HP H5Nx in poultry in the United States and Canada, providing additional evidence of unresolved mechanisms that restrict the emergence and perpetuation of HP avian influenza viruses in these natural reservoirs.

scholarly journals Trichothecene Profiling and Population Genetic Analysis of Gibberella zeae from Barley in North Dakota and Minnesota

2011 ◽  
Vol 101 (6) ◽  
pp. 687-695 ◽  
Author(s):  
Rishi R. Burlakoti ◽  
Stephen M. Neate ◽  
Tika B. Adhikari ◽  
Sanjaya Gyawali ◽  
Bacilio Salas ◽  
...  
Keyword(s):  
United States ◽  
Spatial Structure ◽  
North Dakota ◽  
Population Genetic ◽  
The United States ◽  
Gibberella Zeae ◽  
Upper Midwest ◽  
High Gene ◽  
Pcr Assays ◽  
Made In

Gibberella zeae, the principal cause of Fusarium head blight (FHB) of barley, contaminates grains with several mycotoxins, which creates a serious problem for the malting barley industry in the United States, China, and Europe. However, limited studies have been conducted on the trichothecene profiles and population genetic structure of G. zeae isolates collected from barley in the United States. Trichothecene biosynthesis gene (TRI)-based polymerase chain reaction (PCR) assays and 10 variable number tandem repeat (VNTR) markers were used to determine the genetic diversity and compare the trichothecene profiles of an older population (n = 115 isolates) of G. zeae collected in 1997 to 2000 with a newer population (n = 147 isolates) collected in 2008. Samples were from across the major barley-growing regions in North Dakota and Minnesota. The results of TRI-based PCR assays were further validated using a subset of 32 and 28 isolates of G. zeae by sequence analysis and gas chromatography, respectively. TRI-based PCR assays revealed that all the G. zeae isolates in both populations had markers for deoxynivalenol (DON), and the frequencies of isolates with a 3-acetyldeoxynivalenol (3-ADON) marker in the newer population were ≈11-fold higher than those among isolates in the older population. G. zeae populations from barley in the Midwest of the United States showed no spatial structure, and all the isolates were solidly in clade 7 of G. zeae, which is quite different from other barley-growing areas of world, where multiple species of G. zeae are commonly found in close proximity and display spatial structure. VNTR analysis showed high gene diversity (H = 0.82 to 0.83) and genotypic diversity but low linkage disequilibrium (LD = 0.02 to 0.07) in both populations. Low genetic differentiation (FST = 0.013) and high gene flow (Nm = 36.84) was observed between the two populations and among subpopulations within the same population (Nm = 12.77 to 29.97), suggesting that temporal and spatial variations had little influence on population differentiation in the Upper Midwest. Similarly, low FST (0.02) was observed between 3-ADON and 15-acetyldeoxynivalenol populations, indicating minor influence of the chemotype of G. zeae isolates on population subdivision, although there was a rapid increase in the frequencies of isolates with the 3-ADON marker in the Upper Midwest between the older collection made in 1997 to 2000 and the newer collection made in 2008. This study provides information to barley-breeding programs for their selection of isolates of G. zeae for evaluating barley genotypes for resistance to FHB and DON accumulation.

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