The effects of feeding mixed tocopherol oil on whole-blood respiratory burst and neutrophil immunometabolic-related gene expression in lactating dairy cows

Background: Alterations in gene expression in the peripheral blood of patients with acute stroke have been demonstrated using microarray technology. Whole blood and peripheral blood mononuclear cells (PBMCs) were used in prior studies in which panels of genes diagnostic for stroke were developed. We aimed to determine the cellular sources of alterations in gene expression by studying individual leukocyte subsets. Methods: The expression of four genes previously found to be upregulated in ischemic and hemorrhagic stroke (IL1R2, S100A9, ETS2 and F5) was measured in four leukocyte subsets: CD14+ monocytes, CD4+ T cell lymphocytes, CD20+ B cell lymphocytes and PBMCs. These four genes had been reported in at least two of the previously published stroke-related gene panels. Peripheral blood was obtained from six acute stroke patients (all <48 hours from symptom onset) and 6 age, race and sex matched control subjects. Leukocytes were separated from whole blood using density gradient centrifugation and column magnetic bead cell sorting. The purity of separated leukocyte subsets exceeded 90% and was verified with flow cytometry. Messenger RNA was isolated from each leukocyte subset and analyzed by two step RT PCR and qPCR. The expression of the four stroke-related genes was compared to the expression of a housekeeping gene (GAPDH). The relative expression of individual genes and of the 4 gene panel within cellular subsets was compared between stroke patients and control subjects. Results: Individually, IL1R2 and S100A9 were significantly over-expressed in stroke patients with a 10 fold increase for IL1R2 in PBMCs (p<0.05) and a 3 fold increase for S100A9 in the CD4+ T and CD20+ B lymphocyte subsets (p<0.05). When analyzed as a panel of four genes the expression of IL1R2, S100A9, ETS2 and F5 was significantly higher in both the CD4+ T lymphocytes (p<0.05) and CD20+ B lymphocytes (p<0.05) of stroke patients but not in the monocytes or the PBMCs. Conclusion: These results show the potential diagnostic value of selected genes from panels previously found in microarray studies in stroke patients. They also emphasize the value of panel analysis over that of single gene expression and the potential cellular specificity of alterations in gene expression. Analysis of whole blood and PBMCs alone may not reflect important dynamic changes in stroke-related gene expression.

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Effects of isonitrogenous and isocaloric total mixed ration composed of forages with different quality on milk fatty acid composition and gene expression of mammary lipogenic enzymes in mid‐lactating dairy cows

Animal Science Journal ◽

10.1111/asj.13154 ◽

2018 ◽

Vol 90 (2) ◽

pp. 214-221 ◽

Cited By ~ 3

Author(s):

Hang Zhang ◽

Changjin Ao ◽

Khas‐Erdene ◽

Ni Dan

Keyword(s):

Gene Expression ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Dairy Cows ◽

Acid Composition ◽

Milk Fatty Acid ◽

Lipogenic Enzymes ◽

Total Mixed Ration ◽

Lactating Dairy Cows

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445 Toxy-Nil and Unike Plus modulate differences in gene expression of milk somatic cells isolated from mammary gland of lactating dairy cows fed aflatoxin B1

Journal of Animal Science ◽

10.2527/asasann.2017.445 ◽

2017 ◽

Vol 95 (suppl_4) ◽

pp. 218-219

Author(s):

R. O. Rodrigues ◽

D. R. Ledoux ◽

G. E. Rottinghaus ◽

R. Borutova ◽

...

Keyword(s):

Gene Expression ◽

Mammary Gland ◽

Dairy Cows ◽

Aflatoxin B1 ◽

Somatic Cells ◽

Lactating Dairy Cows ◽

Milk Somatic Cells

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Lipopolysaccharide derived from the digestive tract activates inflammatory gene expression and inhibits casein synthesis in the mammary glands of lactating dairy cows

Oncotarget ◽

10.18632/oncotarget.7371 ◽

2016 ◽

Vol 7 (9) ◽

pp. 9652-9665 ◽

Cited By ~ 17

Author(s):

Kai Zhang ◽

Guangjun Chang ◽

Tianle Xu ◽

Lei Xu ◽

Junfei Guo ◽

...

Keyword(s):

Gene Expression ◽

Dairy Cows ◽

Digestive Tract ◽

Mammary Glands ◽

Inflammatory Gene Expression ◽

Inflammatory Gene ◽

Lactating Dairy Cows ◽

Casein Synthesis

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Respiratory burst activity of blood and milk neutrophils in dairy cows during different stages of lactation

Journal of Dairy Research ◽

10.1017/s0022029901005039 ◽

2001 ◽

Vol 68 (3) ◽

pp. 399-415 ◽

Cited By ~ 65

Author(s):

JALIL MEHRZAD ◽

HILDE DOSOGNE ◽

EVELYNE MEYER ◽

ROGER HEYNEMAN ◽

CHRISTIAN BURVENICH

Keyword(s):

Dairy Cows ◽

Respiratory Burst ◽

Polymorphonuclear Leukocytes ◽

Post Partum ◽

Burst Activity ◽

Early Lactation ◽

Respiratory Burst Activity ◽

Lactating Dairy Cows

The non-stimulated and phorbol 12-myristate 13-acetate (PMA)-stimulated luminol-augmented cellular chemiluminescence (CL) response and viability of milk and blood polymorphonuclear leukocytes (PMN) were determined in lactating dairy cows during different stages of lactation. In the first study, ten healthy cows each in early, mid and late lactation were compared. In a second study, the same measurements as in the first study were evaluated longitudinally in 12 cows during 1 month following parturition. The CL activity and myeloperoxidase (MPO) content of milk PMN and macrophages (M) were also compared. Milk M did not possess MPO activity and were devoid of any luminol-enhanced CL. The CL activity of milk and blood PMN was significantly lower in early lactation than in mid and late lactation (P < 0·001). Whereas little changes were observed in viability of blood PMN, the viability of milk PMN was lower in early lactation than in mid and late lactation (P < 0·001). The percentage of PMN in isolated milk cells was also lower during early lactation than during mid and late lactation (P < 0·001). The CL activity in response to PMA during early, mid and late lactation increased 13, 59 and 42-fold in blood PMN and 1·7, 2·6 and 2·4-fold in milk PMN, respectively, in comparison with non-stimulated PMN. The CL activity, both in milk and blood PMN, the milk PMN viability and the percentage of milk PMN were lowest between 3 d and 11 d post partum. These observed changes immediately after calving could contribute to a higher susceptibility to mastitis in that period.

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