Prophylaxis of the laminectomy membrane

1978 ◽  
Vol 49 (3) ◽  
pp. 419-424 ◽  
Author(s):  
José Barberá ◽  
José Gonzalez ◽  
José Esquerdo ◽  
Jaime Broseta ◽  
Juan Luis Barcia-Salorio
Keyword(s):  
Side Effects ◽  
Bone Graft ◽  
Periodic Acid ◽  
Scar Tissue ◽  
Control Group ◽  
Periodic Acid Schiff ◽  
Content Type ◽  
Laminectomy Membrane ◽  
Hematoxylin And Eosin ◽  
Fine Print

✓ Sixty laminectomies were performed in dogs to investigate the prevention of the laminectomy membrane and its side effects. These operations were distributed in six groups of 10, one was a control group, and in the others the bone defect was protected with different materials (Oxicel, Silastic, Dacron, methyl methacrylate, and Kiel bone graft). After a survival of 2 months, suboccipital myelography was performed, immediately after which the animals were sacrificed and the operated spinal slice obtained. The different radiographic densities of the tissues of each slice were calculated and the diverse histological nature identified by hematoxylin and eosin, periodic acid Schiff, Masson's trichrome, and Gomori's reticulin stains. Only the acrylic plastic and the Kiel bone graft prevented expansion of the scar tissue inside the spinal canal and adhesions between the dura and the cicatricial overlying muscles. Therefore, the authors suggest that a solid barrier is necessary to effectively prevent the so-called “laminectomy membrane.”

The fate of autogenous grafts to the spinal dura

1978 ◽  
Vol 49 (3) ◽  
pp. 412-418 ◽  
Author(s):  
Jeffrey T. Keller ◽  
Stewart B. Dunsker ◽  
Joe M. McWhorter ◽  
Carlos M. Ongkiko ◽  
Mary C. Saunders ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Picric Acid ◽  
Periodic Acid ◽  
Scar Tissue ◽  
Erector Spinae ◽  
Colloidal Iron ◽  
Periodic Acid Schiff ◽  
Content Type ◽  
Tissue Attachment ◽  
Fine Print

✓ An experimental study was designed to compare the efficacy of autogenous fat and Gelfoam in minimizing scar tissue attachment to the dura and nerve roots. A multiple level lumbar laminectomy was performed in 20 dogs, and Gelfoam and autogenous fat were placed in the epidural space at two laminectomy sites, over the dura, interposed between the overlying paraspinal muscles and the dura. A third lumbar laminectomy site in each dog served as a control. The animals were sacrificed after different time periods (3, 6, 12, 18, and 24 weeks), and the specimens were examined histologically and histochemically. The colloidal iron-periodic acid Schiff-Bismarck brown-picric acid (CI-PAS-BB-PA) and high iron diamine-Alcian blue 8Gx (HID-AB) techniques were used to demonstrate connective tissues and glycosaminoglycans. The scar at the laminectomy sites where Gelfoam was placed and at the control sites was composed of dense irregular connective tissue. Examination of the laminectomy sites where autogenous fat was used revealed less scar tissue. In addition, there was a layer of fat with a slight increase in intercellular connective tissue fibers interposed between the overlying erector spinae musculature and the dura. Our results indicate that autogenous fat interposed between the dura and the overlying paraspinal musculature serves as a barrier limiting the growth of connective tissue into the spinal canal. We could find no evidence that Gelfoam prevented the ingrowth of scar tissue into the operated area.

Enterogenous cyst in the cervical spinal canal

1988 ◽  
Vol 68 (2) ◽  
pp. 292-296 ◽  
Author(s):  
Kouichi Miyagi ◽  
Jiro Mukawa ◽  
Susumu Mekaru ◽  
Yasunari Ishikawa ◽  
Toshihiko Kinjo ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Immunohistochemical Staining ◽  
Periodic Acid ◽  
Enterogenous Cyst ◽  
Resonance Imaging ◽  
Periodic Acid Schiff ◽  
Content Type ◽  
Low Intensity ◽  
Old Japanese ◽  
Fine Print

✓ The case of an 11-year-old Japanese girl with an intradural and extramedullary enterogenous cyst is presented. A mass giving a low-intensity signal in comparison with the spinal cord was demonstrated on magnetic resonance imaging. Histologically, the diagnosis was confirmed on specimens stained with periodic acid-Schiff, alcian blue, mucicarmine, and immunohistochemical staining of carcinoembryonic antigen, and by electron microscopy.

Impact of acute fonofos exposure on skeletal muscle of zebrafish: Histopathological and biometric analyses

2021 ◽  
Vol 57 ◽  
pp. 25
Author(s):  
Sezgi Arman ◽  
Sema İşisağ Üçüncü
Keyword(s):  
Skeletal Muscle ◽  
Periodic Acid ◽  
Ache Inhibitor ◽  
Control Group ◽  
Dependent Manner ◽  
Organophosphate Insecticide ◽  
Periodic Acid Schiff ◽  
Concentration Dependent Manner ◽  
Pectoral Region ◽  
Hematoxylin And Eosin

It is widely known that pesticides generally do not show target specificity, and off-target species are strikingly affected by these chemicals. In the current work, histological changes in skeletal muscles of zebrafish (Danio rerio) caused by fonofos, an acetylcholinesterase (AChE) inhibitor organophosphate insecticide, were examined. Zebrafish were treated with 1 mg/L, 2 mg/L and 4 mg/L of fonofos for 96 hours. Skeletal muscle samples were removed from the pectoral region and embedded in paraffin. Sections were stained with Mayer's Hematoxylin and Eosin, Gomori's Trichrome and Periodic Acid Schiff techniques. Histopathological alterations were investigated by light microscopy. Fibrosis, intramyofibrillar vacuoles, disintegrated myofibrils, splitting of myofibers, atrophic and disappeared fibers, histoarchitectural loss, necrosis and progressive decrement in glycogen content were noted. Muscle fiber diameter measurements were also performed. Statistical analysis showed that measured fiber diameters of all fonofos exposed groups were significantly different from the control group, and they decreased in a concentration-dependent manner. These results suggested that fonofos caused significant myoarchitectural impairments in non-target freshwater zebrafish.

Prophylaxis of postmyelogram headaches

1978 ◽  
Vol 49 (6) ◽  
pp. 869-871 ◽  
Author(s):  
Paul Gutterman ◽  
Honorio S. Bezier
Keyword(s):  
Side Effects ◽  
Control Group ◽  
Content Type ◽  
Fine Print

✓ One hundred patients undergoing myelography received prophylactic autologous epidural blood patches to prevent postmyelogram headache. The results indicate a significant reduction in the incidence and severity of this problem compared to a control group, without any significant side effects.

Combined effects of acetaminophen, isopropylantipyrine and caffeine on pregnant and nonpregnant liver

2001 ◽  
Vol 20 (11) ◽  
pp. 569-575 ◽  
Author(s):  
F Burdan ◽  
Z Siezieniewska ◽  
Z Urbanowicz
Keyword(s):  
Periodic Acid ◽  
Tween 80 ◽  
Control Group ◽  
Periodic Acid Schiff ◽  
Turbidity Test ◽  
Female Wistar Rats ◽  
Hematoxylin And Eosin ◽  
Thymol Turbidity Test ◽  
Determination Of Activity

The common effects of acetaminophen (APA), isopropylantipyrine (IPA) and caffeine on liver were examined in rats. The preparations were given in Tween-80 solution once daily, in a constant proportion of 5:3:1, during days 8 to14 of gestation (S1, S2, S3 groups) or between days 8 and 14 of the experiment in nonpregnant female Wistar rats (S1-NP, S2-NP, S3-NP groups). The administration was in three different doses: doses S1, S1-NP — 3.5 mg/kg APA, 2.14 mg/ kg IPA, 0.7 mg/kg caffeine; doses S2, S2-NP were 10 times higher; and doses S3, S3-NP 100 times higher than doses S1, S1-NP. There were two control groups (T, T-NP) with Tween-80. At day 21 of gestation/experiment blood was taken for determination of activity of alanine (ALA) and aspartate (AST) aminotransferase, lactate (LDH) and glutamate (GLDH) dehydrogenase, levels of bilirubin (BIL), urea (URE), total protein (TP) and thymol turbidity test (TTT). The liver sections were examined by light microscopy with four stains: hematoxylin and eosin (H+E), silver Gomori, van Gieson and periodic acid-Schiff (PAS). There was a statistically significant (P<0.05) increase in the GLDH (S3-NP, S2, S3 groups) and AST, LDH (S3 group) activity, a decrease in URE (S2, S3 groups) and decrease in TP (S3-NP, S2, S3 groups) compared to the corresponding control group. Significant differences were noted in activity of AST, GLDH, and levels of the URE and TP between pregnant and nonpregnant females. The treatment resulted in minimal reactive and degenerative changes in light microscopic pattern of liver.

Presence and significance of NK cells in glioblastomas

1989 ◽  
Vol 70 (5) ◽  
pp. 728-731 ◽  
Author(s):  
Jesús Vaquero ◽  
Santiago Coca ◽  
Santiago Oya ◽  
Roberto Martínez ◽  
Josefa Ramiro ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Nk Cells ◽  
Survival Time ◽  
Tumor Cells ◽  
Lymphocytic Infiltration ◽  
Surface Marker ◽  
Content Type ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Fine Print

✓ A monoclonal antibody against the surface marker IOT-10 of natural killer (NK) cells was used to investigate the presence of these cells in a series of 25 glioblastomas. In 40% of the tumors, IOT-10-positive NK cells were found in small numbers scattered among the tumor cells. The presence of IOT-10-positive NK cells was not related to the degree of lymphocytic infiltration in the tumor as demonstrated by hematoxylin and eosin staining, nor did it appear to influence the survival time of the patients studied.

scholarly journals The potential renal toxicity of silver nanoparticles after repeated oral exposure and its underlying mechanisms

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hamed Nosrati ◽  
Manijeh Hamzepoor ◽  
Maryam Sohrabi ◽  
Massoud Saidijam ◽  
Mohammad Javad Assari ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Molecular Mechanisms ◽  
Renal Toxicity ◽  
Periodic Acid ◽  
Critical Role ◽  
Oral Exposure ◽  
Control Group ◽  
Rt Pcr ◽  
Periodic Acid Schiff

Abstract Background Silver nanoparticles (AgNPs) can accumulate in various organs after oral exposure. The main objective of the current study is to evaluate the renal toxicity induced by AgNPs after repeated oral exposure and to determine the relevant molecular mechanisms. Methods In this study, 40 male Wistar rats were treated with solutions containing 30, 125, 300, and 700 mg/kg of AgNPs. After 28 days of exposure, histopathological changes were assessed using hematoxylin-eosin (H&E), Masson’s trichrome, and periodic acid-Schiff (PAS) staining. Apoptosis was quantified by TUNEL and immunohistochemistry of caspase-3, and the level of expression of the mRNAs of growth factors was determined using RT-PCR. Results Histopathologic examination revealed degenerative changes in the glomeruli, loss of tubular architecture, loss of brush border, and interrupted tubular basal laminae. These changes were more noticeable in groups treated with 30 and 125 mg/kg. The collagen intensity increased in the group treated with 30 mg/kg in both the cortex and the medulla. Apoptosis was much more evident in middle-dose groups (i.e., 125 and 300 mg/kg). The results of RT-PCR indicated that Bcl-2 and Bax mRNAs upregulated in the treated groups (p < 0.05). Moreover, the data related to EGF, TNF-α, and TGF-β1 revealed that AgNPs induced significant changes in gene expression in the groups treated with 30 and 700 mg/kg compared to the control group. Conclusion Our observations showed that AgNPs played a critical role in in vivo renal toxicity.

scholarly journals Deep learning-based transformation of H&E stained tissues into special stains

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kevin de Haan ◽  
Yijie Zhang ◽  
Jonathan E. Zuckerman ◽  
Tairan Liu ◽  
Anthony E. Sisk ◽  
...  
Keyword(s):  
Visual Inspection ◽  
Kidney Diseases ◽  
Periodic Acid ◽  
Needle Core Biopsy ◽  
Silver Stain ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Biopsy Tissue ◽  
Hematoxylin And Eosin

AbstractPathology is practiced by visual inspection of histochemically stained tissue slides. While the hematoxylin and eosin (H&E) stain is most commonly used, special stains can provide additional contrast to different tissue components. Here, we demonstrate the utility of supervised learning-based computational stain transformation from H&E to special stains (Masson’s Trichrome, periodic acid-Schiff and Jones silver stain) using kidney needle core biopsy tissue sections. Based on the evaluation by three renal pathologists, followed by adjudication by a fourth pathologist, we show that the generation of virtual special stains from existing H&E images improves the diagnosis of several non-neoplastic kidney diseases, sampled from 58 unique subjects (P = 0.0095). A second study found that the quality of the computationally generated special stains was statistically equivalent to those which were histochemically stained. This stain-to-stain transformation framework can improve preliminary diagnoses when additional special stains are needed, also providing significant savings in time and cost.

Evaluation of endorphin content in the CSF of patients with trigeminal neuralgia before and after Gasserian ganglion thermocoagulation

1981 ◽  
Vol 55 (6) ◽  
pp. 935-937 ◽  
Author(s):  
Giuseppe Salar ◽  
Salvatore Mingrino ◽  
Marco Trabucchi ◽  
Angelo Bosio ◽  
Carlo Semenza
Keyword(s):  
Cerebrospinal Fluid ◽  
Trigeminal Neuralgia ◽  
Control Group ◽  
Gasserian Ganglion ◽  
Content Type ◽  
Group Of Seven ◽  
Idiopathic Trigeminal Neuralgia ◽  
Significant Difference ◽  
Before And After ◽  
Fine Print

✓ The β-endorphin content in cerebrospinal fluid (CSF) was evaluated in 10 patients with idiopathic trigeminal neuralgia during medical treatment (with or without carbamazepine) and after selective thermocoagulation of the Gasserian ganglion. These values were compared with those obtained in a control group of seven patients without pain problems. No statistically significant difference was found between patients suffering from trigeminal neuralgia and those without pain. Furthermore, neither pharmacological treatment nor surgery changed CSF endorphin values. It is concluded that there is no pathogenetic relationship between trigeminal neuralgia and endorphins.

The effects of dexamethasone on bone fusion in an experimental model of posterolateral lumbar spinal arthrodesis

2001 ◽  
Vol 94 (1) ◽  
pp. 76-81 ◽  
Author(s):  
Paul D. Sawin ◽  
Curtis A. Dickman ◽  
Neil R. Crawford ◽  
M. Stephen Melton ◽  
William D. Bichard ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Bone Graft ◽  
Spinal Fusion ◽  
Experimental Model ◽  
Content Type ◽  
Bone Fusion ◽  
Group 12 ◽  
Spinal Arthrodesis ◽  
Lumbar Spinal ◽  
Fine Print

Object. The use of corticosteroid agents during the healing phase after spinal arthrodesis remains controversial. Although anecdotal opinion suggests that corticosteroids may inhibit bone fusion, such an effect has not been substantiated in clinical trials or laboratory investigations. This study was undertaken to delineate the effect of exogenous corticosteroid administration on bone graft incorporation in an experimental model of posterolateral lumbar fusion. Methods. An established, well-validated model of lumbar intertransverse process spinal fusion in the rabbit was used. Twenty-four adult New Zealand white rabbits underwent L5–6 bilateral posterolateral spinal fusion in which autogenous iliac crest bone graft was used. After surgery, the animals were randomized into two treatment groups: a control group (12 rabbits) that received intramuscular injections of normal saline twice daily and a dexamethasone group (12 rabbits) that received intramuscular dexamethasone (0.05 mg/kg) twice daily. After 42 days, the animals were killed and the integrity of the spinal fusions was assessed by radiography, manual palpation, and biomechanical testing. In seven (58%) of the 12 control rabbits, solid posterolateral fusion was achieved. In no dexamethasone-treated rabbits was successful fusion achieved (p = 0.003). Tensile strength and stiffness of excised spinal segments were significantly lower in dexamethasone-treated animals than in control animals (tensile strength 91.4 ± 30.6 N and 145.3 ± 48.2, respectively, p = 0.004; stiffness 31.4 ± 11.6 and 45.0 ± 15.2 N/mm, respectively, p = 0.02). Conclusions. The corticosteroid agent dexamethasone inhibited bone graft incorporation in a rabbit model of single-level posterolateral lumbar spinal fusion, inducing a significantly higher rate of nonunion, compared with that in saline-treated control animals.

Sign in / Sign up

Export Citation Format

Share Document