scholarly journals Histopathological Study of Endoscopic Biopsies

1970 ◽  
Vol 52 (190) ◽  
Author(s):  
Rupendra Thapa ◽  
Mamta Lakhey ◽  
Pradeep Kumar Yadav ◽  
Prakash Kandel ◽  
Choodamani Aryal ◽  
...  
Keyword(s):  
Chronic Gastritis ◽  
Histopathological Study ◽  
Chronic Active Gastritis ◽  
Endoscopic Biopsies ◽  
Neoplastic Lesion ◽  
Neoplastic Lesions ◽  
Gastric Biopsies ◽  
H Pylori ◽  
One Year ◽  
Upper Gastrointestinal

Introduction: Diseases of upper gastrointestinal tract are responsible for a great deal of morbidity and mortality. The histopathological study of endoscopic biopsies permits exact diagnosis for further management.The aim of this study is to find out the histopathological pattern of endoscopic biopsy.Methods: A descriptive study was conducted in consecutive endoscopic gastric biopsies in a Hospital over a period of one year. The patient of all ages and both sexes who underwent gastric biopsy during the study period was included.Results: Endoscopic biopsies were studied on patients of age ranging from 20 years female to 84 years male. The histopathology revealed non-neoplastic lesions 54 (67.5%) and neoplastic lesions 26 (32.5%). Most common non-neoplastic lesion noted was mild chronic gastritis 22(27.5%) followed by chronic active gastritis 15 (18.75%) while H. pylori was present in 13 (16.25%) and absent in 2 (2.5%).Conclusions: Mild chronic gastritis was the commonest lesion noted in non-neoplastic lesions and adenocarcinoma was the commonest neoplastic lesion in the endoscopic gastric biopsies.______________________________________________________________________________________Keywords: biopsy; endoscopy; gastritis; histopathological.

scholarly journals Histopathological Study of Upper Gastrointestinal Tract Endoscopic Biopsies

2019 ◽  
Vol 6 (1) ◽  
pp. 42-46
Author(s):  
Mosammat Suchana Nazrin ◽  
Nur E Jannatul Ferdous ◽  
Madhusudan Saha ◽  
Fahmi Iqbal Rabbi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Gastrointestinal Tract ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Upper Gastrointestinal Tract ◽  
North East ◽  
Endoscopic Biopsies ◽  
Neoplastic Lesion ◽  
Neoplastic Lesions ◽  
Upper Gastrointestinal

Background: Diseases of gastrointestinal tract are a major cause of morbidity and mortality. Objective: This study was carried out to determine the spectrum of histopathological lesions of upper gastrointestinal tract. Methodology: This retrospective study was conducted in the Department of Pathology at North East Medical College, Sylhet, Bangladesh during a 36 months period from January 2013 to December 2015. Endoscopies were performed using Olympus 150, forward viewing upper GI endoscope. The biopsy specimens received were fixed in 10.0% formalin and routinely processed in Haematoxyline & Eosin stain. Results: The present study included 135 endoscopic biopsies. The mean age with SD of the study population was 53.20±16.09 years. Among 53 cases of esophageal biopsies 8(15.08%) cases showed non-neoplastic lesions and 45(84.92%) cases were neoplastic of which 39(73.6%) cases and 6(11.32%) cases were squamous cell carcinoma and adenocarcinoma respectively. Among 6 cases of gastro esophageal junction biopsies 3(50%) cases showed non-neoplastic lesions and 3(50%) were neoplastic, of which 2(33.33%) were adenocarcinoma and 1(16.67%) were squamous cell carcinoma. Among 61 cases of stomach biopsies 34(55.74%) showed non-neoplastic lesions and 27(44.26%) were neoplastic, of which 1(1.64%) case was adenoma and 25(40.98%) were adenocarcinoma. Among 15 cases of duodenal biopsies 13(86.67%) cases showed non-neoplastic lesions and 2(13.33%) were neoplastic one of which was adenocarcinoma (6.67%). Among 135 cases endoscopist reported 82(60.74%) cases as neoplastic and 53(39.26%) as non-neoplastic, whereas histopathology revealed 77(57.03%) cases neoplastic and 58(42.97%) cases non-neoplastic. Conclusion: Common site of upper GIT endoscopic biopsy is stomach which are mostly neoplastic lesion; however, most common malignancy is squamous cell carcinoma of the oesophagus. Journal of Current and Advance Medical Research 2019;6(1):42-46

Histopathological Study of Cystoscopic Bladder Biopsies in a Tertiary Care Center

2021 ◽  
Vol 6 (1) ◽  
pp. 1310-1314
Author(s):  
Rachana Dhakal ◽  
Hem Nath Joshi ◽  
Ramesh Makaju ◽  
Shailendra Sigdel
Keyword(s):  
Urinary Bladder ◽  
Urothelial Carcinoma ◽  
Pathological Condition ◽  
Care Center ◽  
Tertiary Care ◽  
University Hospital ◽  
World Health ◽  
Histopathological Study ◽  
Neoplastic Lesion ◽  
Neoplastic Lesions

Introduction: Non-neoplastic urinary bladder lesions are not life threatening, but are an important source to cause clinical symptoms and signs. However, neoplasms of the bladder are a source of morbidity, mortality, and exhibit more clinical challenges. Cystoscopy provides overall information about an anatomical/pathological condition of the urinary bladder which will be helpful for patients' management. A cystoscopic biopsy is a primary diagnostic tool for diagnosing urinary bladder cancer. Objectives: The objective of the study was to find the frequency and histomorphological characteristics of urinary bladder lesions in Dhulikhel Hospital, to find the clinical presentation of urinary bladder lesions and to grade the urothelial tumors based on the World Health Organization (WHO)/ International Society of Urological Pathology (ISUP) classification 2004. Methodology: This was a retrospective, cross-sectional, observational study carried out in the Department of Pathology, Dhulikhel Hospital-Kathmandu University Hospital. Convenient sampling was done. All cystoscopy biopsies received from January 2014 to December 2018 were studied. All tissue blocks were retrieved, cut, and stained with Hematoxylin and Eosin. The stained slides were examined under a light microscope by the primary investigator. Result: A total of 70 cystoscopic biopsies were analyzed. The majority of patients 20 (28.6%) were in the age group between 60 and 69 years and males were predominant 43 (61.4%). The neoplastic lesions constituted 40 (57.1%) of all bladder lesions, among them urothelial carcinoma accounted for 38 (54.2%). Similarly, chronic cystitis 27(38.6%) was the most common non-neoplastic lesion. Conclusion: The study found that the neoplastic lesions were commonly encountered in urinary bladder lesions. Among them, lowgrade urothelial carcinoma was the most common bladder tumor. However, most of the non-neoplastic lesions were inflammatory in origin. Cystoscopy combined with histomorphological examination helps in the early detection of bladder lesions. 

scholarly journals Increased Resistance of Fluoroquinolones among H. pylori isolates from Patients with Gastroduodenal Diseases in Jordan

2020 ◽  
Vol 17 ◽  
Keyword(s):  
Antimicrobial Agents ◽  
Culture Media ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Clinical Strains ◽  
Chronic Active Gastritis ◽  
Gastric Biopsies ◽  
Stool Antigen ◽  
H Pylori ◽  
Emergence Of Resistance

Recently, Helicobacter pylori has been connected to more than 80% of chronic active gastritis and other gastroduodenal diseases worldwide. Treatment of H. pylori is routinely dependent on the use of multiple antimicrobial agents however, recent data showed the emergence of resistance among clinical strains especially against metronidazole and clarithromycin. This study was conducted to investigate the rate of resistance to different antibiotics that are routinely used in the first line and second line therapies including ciprofloxacin and levofloxacin, tetracycline, amoxicillin, clarithromycin and metronidazole among H. pylori strains isolated from patients with gastroduodenal diseases in Jordan. Both antral and corpus mucosal biopsies from the stomach of patients with positive results of H. pylori stool antigen and urease breath tests were used for the isolation of H. pylori on selective culture media. The standard agar diffusion method was performed to determine the sensitivity of H. pylori clinical isolates against ciprofloxacin and levofloxacin according to CLSI. Among 62 H. pylori clinical strains isolated from gastric biopsies, 21% and 11% were resistant to levofloxacin and ciprofloxacin respectively. Resistance to metronidazole and clarithromycin was found in 90% and 11% respectively. No resistance was observed against amoxicillin, tetracycline and gemifloxacin. The following MIC90 (mg/L) of resistant strains results were obtained at neutral pH 7.3, 64 for metronidazole, 2 for clarithromycin, 2 for ciprofloxacin and 1 for levofloxacin. The present study reported the emergence of increased resistance of fluoroquinolones among H. pylori clinical isolates in Jordan. Concern should be taken into consideration when triple and quadruple therapy regimens are applied for the management of H. pylori infections in our region

scholarly journals Association of Helicobacter pylori infection with nodular antritis and follicular gastritis

2006 ◽  
Vol 63 (3) ◽  
pp. 313-315 ◽  
Author(s):  
Ratko Tomasevic ◽  
Gradimir Golubovic ◽  
Miroslav Kiurski ◽  
Dragana Stankovic ◽  
Radoje Doder ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Case Report ◽  
Chronic Gastritis ◽  
Upper Gastrointestinal Endoscopy ◽  
Antral Mucosa ◽  
Urease Test ◽  
Sydney System ◽  
H Pylori ◽  
The Relationship ◽  
Upper Gastrointestinal

Introduction. Helicobacter pylori (H. pylori) infection is known to be the must common cause of chronic gastritis having some endoscopic and pathologic characteristies as determinated by the Sydney System for Gastritis Classification. The aim of our case report was to point out the relationship between an endoscopic finding of nodular antritis and the presence of H. pylori infection and active chronic gastritis. Case report. Our patient underwent upper gastrointestinal endoscopy for dyspeptic complaints and was diagnosed as having nodular antritis, but also underwent urease test and hystopathologic examination of antral mucosa, to determine the presence and density of H. pylori infection and the presence and severity of gastritis. After a course of anti H. pylori treatment, dyspepsia improved and new biopsy specimens obtained two months and six months afterwards revealed no pathological findings. Conclusion. The case report supported the association of H. pylori infection of lymphoid follicles with nodular gastric mucosis.

scholarly journals Spectrum of Lesions in Urinary Bladder- A Histopathological Study

2018 ◽  
Vol 6 (2) ◽  
pp. 24-27 ◽  
Author(s):  
Anita Shah ◽  
Manglesh Srivastava ◽  
Ashok Samdurkar ◽  
Ghanshyam Sigdel
Keyword(s):  
Urinary Bladder ◽  
Early Stage ◽  
Granulomatous Inflammation ◽  
Urinary Bladder Cancer ◽  
Histopathological Study ◽  
Common Source ◽  
Institutional Review ◽  
Neoplastic Lesion ◽  
Neoplastic Lesions ◽  
Urothelial Tumors

Introduction: The lesions of urinary bladder both non-neoplastic and neoplastic pose a common source of both morbidity and mortality. An accurate diagnosis of these lesions requires cystoscopy which allows a direct visualization of the bladder mucosa and biopsies of suspected lesions. Urinary bladder cancer is sixth most common cancer worldwide and represents a heterogeneous group of neoplasms. The current study aimed to study the different bladder lesions and its clinical features to detect it in early stage and as a mainstay option in the diagnosis and follow up.  Materials and methods: This was a retrospective analysis of biopsies of urinary bladder submitted to the department of pathology over a period of 12 months. The study was approved by the institutional review board of the Universal College of Medical Sciences (UCMS-TH). All the urinary bladder biopsies received in the department were included in the study whereas autolysis of specimen and inadequate biopsies were excluded.  Results: Among the 36 cases of urinary bladder lesions, the majority (35.36%) were in age group 61-70 years (22.33%). The patients had combination of lower urinary tract symptoms, the commonest being hematuria. 30.55%  had non-neoplastic lesions and 69.55% had neoplastic lesion. Among non- neoplastic cases, 5.55% had chronic granulomatous inflammation. Most common neoplastic lesions was infiltrating urothelial carcinoma (n=6) followed by non- invasive urothelial neoplasia (n=5).  Conclusion: A variety of lesions occur in urinary bladder and is commonly encountered by pathologist. Hematuria was commonest symptom and the clinicians investigated these patients further, which led to discovery of the urothelial tumors. Identification of these patients has an important impact on prognosis as well as on therapeutic approach.

scholarly journals Histopathological spectrum of upper gastrointestinal endoscopic biopsies

2018 ◽  
Vol 1 (1) ◽  
pp. 67-74
Author(s):  
Suspana Hirachand ◽  
RR Sthapit ◽  
P Gurung ◽  
S Pradhanang ◽  
R Thapa ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Histopathological Examination ◽  
Upper Gastrointestinal Tract ◽  
Medical College ◽  
Chronic Active Gastritis ◽  
Endoscopic Biopsies ◽  
Upper Gastrointestinal Tract Endoscopy ◽  
A Prospective Study ◽  
Upper Gastrointestinal ◽  
Common Malignancy

Background: Upper gastrointestinal tract disorders are one of the most commonly encountered problems in the clinical practice. A variety of disorders can affect the upper gastrointestinal tract. Endoscopy, in combination with biopsy, plays an important role in the exact diagnosis for further management.Objectives: To determine the spectrum of histopathological lesions of upper gastrointestinal tract.Methods: A prospective study was conducted in the Department of Pathology, Kathmandu Medical College and Teaching Hospital, Nepal from January 2015 to December 2016 (2 years).Results: A total 243 endoscopic biopsies were evaluated. Out of which, 219 cases were from gastric, 15 were from esophagus and 9 were from duodenum. Among the gastric biopsies, 77 cases (35.16%) were chronic active gastritis and 27 cases (12.33%) were malignant. The most common malignancy was adenocarcinoma. Among the 15 oesophageal biopsies, 12 cases (80%) were of non-neoplastic and 3 cases (20%) were of neoplastic nature. The most common malignancy was squamous cell carcinoma. Among 9 cases of duodenum biopsies, all were non-neoplastic, of which chronic non-specific duodenitis (66.66%) was the commonest.Conclusion: Endoscopy is incomplete without histopathological examination of biopsy and so, the combinations of methods play an important role in diagnosis and management of upper gastrointestinal tract disorders.Journal of BP Koirala Institute of Health Sciences, Vol. 1, No. 1, 2018, Page: 67-74

CLINICOPATHOLOGICAL STUDY OF GASTRIC BIOPSIES

2021 ◽  
pp. 38-40
Author(s):  
Manu Jethalya ◽  
Saurabh Totla
Keyword(s):  
Clinical Presentation ◽  
Early Stage ◽  
Gastric Biopsy ◽  
Histopathological Study ◽  
Age Group ◽  
Malignant Tumours ◽  
Early Management ◽  
Medical College ◽  
Neoplastic Lesions ◽  
Gastric Biopsies

Background: Stomach is an important site for variety of lesions especially malignant tumours. Gastric biopsies along with clinical prole of cases play an important role in the diagnosis of gastric neoplasm and therefore aids in early management. Aim and Objectives: To study the histopathology of Gastric biopsies and correlate them with clinical presentation, age, sex and to differentiate them between neoplastic and non neoplastic lesions. Materials and Methods: The prospective study was done in the Department of Pathology Geetanjali Medical College and Hospital, Udaipur between 1stJanuary 2019 to 30thJune 2020. A total of 83 gastric biopsies were included in the study and were analyzed along with their clinical proles. Results: A total of 83 gastric biopsies were included in the study. Most commonly affected age group was 50-69 years with 41 patients (49.40%) followed by 40-49 years with 14(16.87%). Out of 83 cases, 58 (69.88%) were males & 25 (30.12%) were females, 26 (31.33%) cases were non neoplastic & 57 (68.67%) cases were neoplastic.Conclusion: The gastric biopsy not only permits exact diagnosis of specic entity but also helps to plan for early medical or surgical therapy. The histopathological study detects mucosal lesions at an early stage especially atrophy, metaplasia and dysplasia as to prevent progress of these lesions to invasive cancer.

scholarly journals Comparative analysis of two-dimensional electrophoresis maps (2-DE) of Helicobacter pylori from Brazilian patients with chronic gastritis and duodenal ulcer: a preliminary report

2006 ◽  
Vol 48 (3) ◽  
pp. 175-177 ◽  
Author(s):  
Diego R.B. Pereira ◽  
Daniel Martins ◽  
Flavia V. Winck ◽  
Marcus B. Smolka ◽  
Nancy F. Nishimura ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Comparative Analysis ◽  
Chronic Gastritis ◽  
Protein Identification ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Duodenal Ulcers ◽  
Pathogenic Factors ◽  
Gastric Biopsies ◽  
H Pylori

Helicobacter pylori is a bacterium recognized as the major cause of peptic ulcer and chronic gastritis. Recently, a proteome-based approach was developed to investigate pathogenic factors related to H. pylori. In this preliminary study, H. pylori strains were isolated from gastric biopsies of patients with chronic gastritis and duodenal ulcers. A partial proteomic analysis of H. pylori strains was performed by bacterial lyses and proteins were separated by two-dimensional gel electrophoresis (2-DE). A comparative analysis was performed to verify a differential protein expression between these two 2-DE maps. These data should be useful to clarify the role of different proteins related to bacterial pathogenesis. This study will be completed using a larger number of samples and protein identification of H. pylori by MALDI-TOF mass spectrometry.

scholarly journals Efficacy of immunohistochemical staining in detecting Helicobacter pylori in Saudi patients with minimal and atypical infection

2021 ◽  
Vol 65 (3) ◽  
Author(s):  
Mohammed Akeel ◽  
Ahmed Elhafey ◽  
Atef Shehata ◽  
Erwa Elmakki ◽  
Thanaa Aboshouk ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Direct Detection ◽  
Giemsa Staining ◽  
Pcr Analysis ◽  
Qrt Pcr ◽  
Urease Test ◽  
Gastric Biopsies ◽  
Polymerase Chain ◽  
H Pylori

Gastric Helicobacter pylori infection is diagnosed based on histopathological evaluation of gastric mucosal biopsies, urease test, urea breath test, H. pylori culturing, or direct detection using polymerase chain reaction (PCR). This study aimed to evaluate the efficacy of immunohistochemical (IHC) staining in detecting H. pylori in gastric biopsies from patients with chronic gastritis and minimal or atypical infection. Gastric biopsies from 50 patients with chronic gastritis were subjected to routine haematoxylin and eosin (H&E), modified Giemsa, and IHC staining. The results of staining were compared with those of quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis identified 32 (64%) H. pylori-positive cases, whereas IHC, H&E, and modified Giemsa staining identified 29 (58%), 27 (54%), and 21 (42%) positive cases. The sensitivity of IHC staining (87.50%) was higher than that of H&E (59.38%) and modified Giemsa (43.75%) staining. The specificity of H&E, modified Giemsa, and IHC staining was 55.56%, 61.11%, and 94.44%, respectively. IHC staining exhibited the highest diagnostic accuracy (90%), followed by H&E (58%) and modified Giemsa (50%) staining. Active gastritis, intestinal metaplasia, and lymphoid follicles were detected in 32 (64%), 4 (8%), and 22 (44%) cases, respectively, and all of these cases were H. pylori positive. In contrast to routine H&E and modified Giemsa staining, IHC allows for the accurate H. pylori detection in cases with minimal or atypical infection. Moreover, IHC can be an alternative diagnostic method to qRT-PCR for detection of H. pylori in such cases.

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