Morphological Characteristics of the Process of Regeneration of Rabbit Bone Tissue Defect Using Paste-Like Composite in the Experiment

The use of porous three-dimensional (3D) composite scaffolds has attracted great attention in bone tissue engineering applications because they closely simulate the major features of the natural extracellular matrix (ECM) of bone. This study aimed to prepare biomimetic composite scaffolds via a simple 3D printing of gelatin/hyaluronic acid (HA)/hydroxyapatite (HAp) and subsequent biomineralization for improved bone tissue regeneration. The resulting scaffolds exhibited uniform structure and homogeneous pore distribution. In addition, the microstructures of the composite scaffolds showed an ECM-mimetic structure with a wrinkled internal surface and a porous hierarchical architecture. The results of bioactivity assays proved that the morphological characteristics and biomineralization of the composite scaffolds influenced cell proliferation and osteogenic differentiation. In particular, the biomineralized gelatin/HA/HAp composite scaffolds with double-layer staggered orthogonal (GEHA20-ZZS) and double-layer alternative structure (GEHA20-45S) showed higher bioactivity than other scaffolds. According to these results, biomineralization has a great influence on the biological activity of cells. Hence, the biomineralized composite scaffolds can be used as new bone scaffolds in bone regeneration.

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Antimicrobial activity of impregnated antibiotic matrix materials for bone tissue defect reconstruction

Archives of the Balkan Medical Union ◽

10.31688/abmu.2020.55.1.02 ◽

2020 ◽

Vol 55 (1) ◽

pp. 22-30

Author(s):

Andrii V. PANTUS ◽

◽

Mykola M ROZHKO ◽

Roman V. KUTSYK ◽

Ruslan V. KOZOVYI ◽

...

Keyword(s):

Antimicrobial Activity ◽

Bone Tissue ◽

Tissue Defect ◽

Defect Reconstruction

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Regeneration of experimental long bone defect after implantation in its cavity of osteoplastic material “Calc-i-oss®”

Journal of Morphological Sciences ◽

10.4322/jms.090215 ◽

2016 ◽

Vol 33 (02) ◽

pp. 099-102

Author(s):

O. Korenkov

Keyword(s):

Bone Tissue ◽

Morphological Characteristics ◽

Compact Bone ◽

Long Bone ◽

Electron Microscopic ◽

Time In Literature ◽

Microscopic Features ◽

Definition Of ◽

Osteoplastic Material ◽

Long Bone Defect

Abstract Introduction: There is a significant divergence of data on the rate of resorption and replacement by the bone tissue of osteoplastic materials based on β-tricalcium phosphate in cancellous bone. At the same time in literature missing morphometric and electron microscopic features of bone tissue of the regenerate of compact substance of bone in these conditions. This study was aimed at the assessment of the healing of compact bone tissue defect after implantation of osteoplastic material “Calc-i-oss®” with the definition of the dynamics of resorption and morphological characteristics of bone tissue of the regenerate. Material and Methods: In the middle third of the diaphysis of the femur of rats there was reproduced the perforated defect to the bone-brain channel that was filled with osteoplastic material “Calc-i-oss®”. After surgery the fragments of injured bones were studied at the 60th and 120th day by methods of light microscopy with morphometry and scanning electron microscopy. Results: The conducted research revealed no inflammatory reaction at the site of the defect, signs of necrobiosis and necrosis of osteocytes in adjacent to the site of implantation maternal bone. The site of defect was filled with lamellar bone tissue high in osteoblasts, osteocytes and with integrated into its structure remains of “Calc-i-oss®”. On the surface and inside the implant there were found osteogenic cells and bone foci. It was established that the osteoplastic material throughout the observation period is subjected to development and replacement by bone tissue of the regenerate, the ratio of which on the 60th day of the experiment was 25.72 ± 2.06% to 74.28 ± 2.06%, and on the 120th day - 18.31 ± 1.54% to 81.69 ± 1.54%. Conclusion: Osteoplastic material “Calc-i-oss®” exhibits biocompatibility, osteoconductive properties, ability to resorption and is replaced by bone tissue, with which it integrates well.

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The expression of cytokines and β -defensin 2, - 3, -4 in rabbit bone tissue after hydroxyapatite (HAp), α- Tricalcium phosphate (α-TCP) and polymethylmethacrylate (PMMA) implantation

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/38/1/012025 ◽

2012 ◽

Vol 38 ◽

pp. 012025

Author(s):

J Vamze ◽

M Pilmane ◽

A Skagers

Keyword(s):

Bone Tissue ◽

Tricalcium Phosphate ◽

Rabbit Bone

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Change of Biomechanical Parameters in the Lower Jaws of Rabbits with Experimental Osteoporosis after Implantation of Calcium-Phosphate Bioceramic Material in the Greater Trochanter Region

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.721.224 ◽

2016 ◽

Vol 721 ◽

pp. 224-228

Author(s):

Girts Salms ◽

Vladislavs Ananjevs ◽

Vladimirs Kasyanovs ◽

Andrejs Skagers ◽

Ilze Salma ◽

...

Keyword(s):

Bone Tissue ◽

Tricalcium Phosphate ◽

Strontium Ranelate ◽

Biomechanical Properties ◽

Control Group ◽

Greater Trochanter ◽

Lower Jaw ◽

Experimental Osteoporosis ◽

Rabbit Bone ◽

Biomechanical Parameters

Investigation of biomechanical properties of the rabbit bone tissue from a corner of the lower jaw was done. Experimental osteoporosis was induced by ovariectomy and following injections of methylprednisolone. The defects in the greater trochanter region was created and filled with granules of a hydroxyapatite and tricalcium phosphate (HAP/TCP 30/70) or HAP/TCP 30/70 together with 5% strontium ranelate. After 3 month animals were euthanased, squared samples have been cut out from a corner of the lower jaw and tested on a bend. Results of research show, that the corner of a lower jaw in rabbit becomes more rigid after filling of defects in the greater trochanter region with granules of a hydroxyapatite and tricalcium phosphate (HAP/TCP 30/70) or granules together with strontium ranelate. The ultimate strain for the bone tissue in the 2nd and 3rd group is less, than for control group. Thus, local uses calcium – phosphatic bioceramic material around the greater trochanter region improves biomechanical parameters of a bone tissue in the lower jaw of animals.

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Influence of cryoconserved placenta preparations on the course of non-specific gonarthrosis in the experiment

TRAUMA ◽

10.22141/1608-1706.2.22.2021.231961 ◽

2021 ◽

Vol 22 (2) ◽

pp. 62-67

Author(s):

O.V. Pelypenko ◽

V.I. Shepitko

Keyword(s):

Knee Joint ◽

Bone Tissue ◽

Inflammatory Process ◽

Morphological Changes ◽

Morphological Characteristics ◽

Biologically Active ◽

Subcutaneous Implantation ◽

Aseptic Inflammation ◽

Intact Group ◽

White Rats

Background. Treatment of joint pathology, both degenerative and traumatic, is a long, multicomponent process that requires joint efforts of the doctor and the patient. The choice of the optimal therapy is complex and ambiguous. Biologically active compounds are one of the most powerful things for fighting with inflammatory process, which is a constant companion of dama-ge. Due to their antioxidant, immunomodulatory and regenerative properties, placental fragments were admitted as a perspective treatment for arthropathies of various etiologies. Purpose of the research: to determine the morphological characteristics of the constituent structures of the knee joints of rats in a comparative aspect under normal conditions, in experimental arthritis and after exposure to a cryopreserved placenta. Material and methods. An experimental study was carried out on 115 white rats. With the intact group (10 animals) we compared 3 control groups of 35 animals each, who underwent: 1) modeling of carrageenan-induced aseptic inflammation of the knee joint; 2) subcutaneous implantation of placental fragment; 3) subcutaneous implantation of placental fragment against the background of aseptic inflammation of the knee joint. Histological preparations were prepared according to the general methods with hematoxylin-eosin and Van Gieson staining. Results. During the creating an inflammatory focus, changes in the cartilaginous tissue were determined from day 3 in the form of lacunarity, karyopyknosis as a stage of necrobiosis of chondrocytes, especially pronounced on days 10–14. Morphological changes in the bone tissue of rats, which were injected λ-carrageenan, were noted on the 7th day of the research in the form of resorption and destruction of bone trabeculae. Later (day 10), the inflammatory process led to a picture of the fragmented architectonics of bone tissue, which was observed up to 21 days. From the side of the synovial membrane, villous hyperplasia, lymphoid infiltration, and the appearance of additional layers of synoviocytes were observed as a result of a proliferative reaction. The most pronounced changes were observed in the integumentary layer of the synovium. After a single injection of a cryopreserved placenta, the signs of inflammation significantly decreased: the area of destruction of cartilage and bone was limited, and signs of mucoid swel-ling in the synovium decreased. Processes of functional blood filling of the microvasculature prevailed over ischemic ones. Stimulation of regenerative processes provided structural recovery on day 21 of observation. Conclusions. Prepartions of cryopreserved placenta influence the course of the inflammatory process in experimental osteoarthritis. The most reliable therapeutic effect is determined from the side of the synovium.

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Bone Tissue Engineering in a Perfusion Bioreactor Using Dexamethasone-Loaded Peptide Hydrogel

Materials ◽

10.3390/ma12060919 ◽

2019 ◽

Vol 12 (6) ◽

pp. 919 ◽

Cited By ~ 1

Author(s):

Marina Panek ◽

Maja Antunović ◽

Lidija Pribolšan ◽

Alan Ivković ◽

Marijan Gotić ◽

...

Keyword(s):

Bone Tissue ◽

Morphological Characteristics ◽

Human Mesenchymal Stem Cells ◽

Collagen I ◽

Perfusion Bioreactor ◽

Engineered Tissue ◽

Polymerase Chain ◽

Main Components ◽

Hematoxylin Eosin

The main goal of this study was the formation of bone tissue using dexamethasone (DEX)-loaded [COCH3]-RADARADARADARADA-[CONH2] (RADA 16-I) scaffold that has the ability to release optimal DEX concentration under perfusion force. Bone-marrow samples were collected from three patients during a hip arthroplasty. Human mesenchymal stem cells (hMSCs) were isolated and propagated in vitro in order to be seeded on scaffolds made of DEX-loaded RADA 16-I hydrogel in a perfusion bioreactor. DEX concentrations were as follows: 4 × 10−3, 4 × 10−4 and 4 × 10−5 M. After 21 days in a perfusion bioreactor, tissue was analyzed by scanning electron microscopy (SEM) and histology. Markers of osteogenic differentiation were quantified by real-time polymerase chain reaction (RT-PCR) and immunocytochemistry. Minerals were quantified and detected by the von Kossa method. In addition, DEX release from the scaffold in a perfusion bioreactor was assessed. The osteoblast differentiation was confirmed by the expression analysis of osteoblast-related genes (alkaline phosphatase (ALP), collagen I (COL1A1) and osteocalcin (OC). The hematoxylin/eosin staining confirmed the presence of cells and connective tissue, while SEM revealed morphological characteristics of cells, extracellular matrix and minerals—three main components of mature bone tissue. Immunocytochemical detection of collagen I is in concordance with given results, supporting the conclusion that scaffold with DEX concentration of 4 × 10−4 M has the optimal engineered tissue morphology. The best-engineered bone tissue is produced on scaffold loaded with 4 × 10−4 M DEX with a perfusion rate of 0.1 mL/min for 21 days. Differentiation of hMSCs on DEX-loaded RADA 16-I scaffold under perfusion force has a high potential for application in regenerative orthopedics.

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The small molecular weight, γ-carboxyglutamic acid-containing protein of rabbit bone tissue

Archives of Oral Biology ◽

10.1016/0003-9969(84)90149-3 ◽

1984 ◽

Vol 29 (12) ◽

pp. 1015-1021 ◽

Cited By ~ 10

Author(s):

J.T. Triffitt ◽

J.M. Wilson

Keyword(s):

Molecular Weight ◽

Bone Tissue ◽

Small Molecular Weight ◽

Carboxyglutamic Acid ◽

Rabbit Bone

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Computational Study of Bone Tissue Mechanical Properties Dependence on Nanoscale Morphological Characteristics

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.752-753.103 ◽

2015 ◽

Vol 752-753 ◽

pp. 103-108

Author(s):

A.S. Semenov ◽

Alexey I. Grishchenko ◽

Boris E. Melnikov

Keyword(s):

Mechanical Properties ◽

Bone Tissue ◽

Computational Study ◽

Morphological Characteristics ◽

Local Stress ◽

Strength Properties ◽

Element Simulation ◽

Bone Substitute Materials ◽

Quasiperiodic Structure ◽

Hydroxyapatite Crystals

A bone tissue is modeled at the nanolevel as a composite with a quasiperiodic structure, consisting of the hydroxyapatite crystals, which are embedded into the collagen fibrils. The aim of the research is to analyze the influence of the bone nanostructure on its mechanical and strength properties. Such studies are important for the creating artificial bone-substitute materials. The influence analysis of the morphological characteristics (hydroxyapatite crystals disorientation, sizes and orientation of mineral bridges, shape of hydroxyapatite crystals, mineralization) on the local stress-strain state and mechanical properties of the representative volume element of bone is carried out by means of the direct finite element simulation and homogenization. The comparison of the obtained results with experimental data demonstrates a good agreement.

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